CHARAK CONFIDENTIAL STANDARD OPERATING PROCEDURE Name of the Instrument: Polarimeter (Make: Rajdhani Scientific Instru
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CHARAK
CONFIDENTIAL STANDARD OPERATING PROCEDURE
Name of the Instrument: Polarimeter (Make: Rajdhani Scientific Instruments) S.O.P. No.: ADI/SAM-001
Issue No.: 4
Page No.: 1 of 2
Date of Issue: March 2006
Date of Review: March 2009
Supercedes: Issue No.3, dated November 2003
1.0 Purpose: This SOP describes the procedure for operating Polarimeter. (Make: Rajdhani Scientific Instruments) 2.0 Responsibility: Analyst 3.0 Precautions: Optical elements must be kept clean and in exact alignment. The match point should be close to the normal zero mark. The field must be evenly illuminated and it should be supplemented by a filtering system capable of isolating the D line from sodium light. Specific attention should be paid to temperature control of the solution and of the polarimeter. Polarimeter tubes should be filled in such a way so as to avoid air bubbles. Particular care is necessary for semi-micro and micro tubes. For tubes with removable end plates fitted with gaskets and caps, tighten the end plates only enough to ensure leak proof seal between the end plate and body of the tube. Liquids and solutions of solids must be clear. Differences between the observed and corrected optical rotation calculated as either specific optical rotation or optical rotation should not be more than ¼th of the range specified in the monograph of the substance. 4.0 Procedure: Sample solution as per monograph is prepared and filled in Polarimeter tube. The tube is inserted in the slot provided ensuring that there is no air bubble in the path of the light and the analyzer is rotated until two halves of field shows equal intensity of light. Adjust the reading with fine adjustment knob such that the cross wires exactly coincide with one of the readings on scale. Reading (optical rotation) x 100 Specific optical rotation = ---------------------------------------------L x C (where, L = length of tube, C = Concentration of the substance in percentage w/v)
Prepared By
Approved By
Issued By
(Ms. Sabina Golatkar) (Senior Executive- Q. A.)
(Mr. Hemant Patil) (Sr. Manager - Corporate Q.A)
(Mr. P. Gopinath) (Gen. Manager-Tech. Services)
CHARAK
CONFIDENTIAL
STANDARD OPERATING PROCEDURE Name of the Instrument: Polarimeter S.O.P. No: ADI/SAM-001 (Make: Rajdhani Scientific Instruments)
Page No: 2 of 2
5.0 Calibration: The polarimeter can be calibrated by measuring the optical rotation in a 2 dm table at 25° using the concentrations of dry sucrose indicated in the table below
Concentration g/ 100 ml
Angle of rotation (+) at 25°
10.0
13.33
20.0
26.61
30.0
39.86
40.0
53.06
50.0
66.23
6.0 Frequency of calibration: Monthly
HISTORY OF CHANGES Issue No.
Date
Remark
1.
March 2000
Original Issue
2.
November 2001
Format revised
3.
November 2003
Reviewed & issued without any change
4.
March 2006
Reviewed & issued without any change
Prepared By
Approved By
Issued By
(Ms. Sabina Golatkar) (Senior Executive- Q. A.)
(Mr. Hemant Patil) (Sr. Manager - Corporate Q.A)
(Mr. P. Gopinath) (Gen. Manager-Tech. Services)