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P/N 679060 Revision 1.0

Allegra X-12/R, X-14/R, X-15R Service Manual

Beckman Coulter, Inc. 250 South Kraemer Blvd. Brea, CA 92821

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Allegra X-12/ R, Allegra X-14/R, Allegra X-15R, Service Manual Table of Contents REVISION HISTORY................................................................................................................................... 3 MANUAL USE............................................................................................................................................. 3 RELATED DOCUMENTS............................................................................................................................ 3 SAFETY INFORMATION............................................................................................................................. 4 1 SYSTEM DESCRIPTION.................................................................................................................. 10 1.1 Specifications Allegra X-12.................................................................................................... 11 1.1.1 Speed............................................................................................................................... 11 1.1.2 Time................................................................................................................................. 11 1.1.3 Temperature..................................................................................................................... 11 1.1.4 Electrical Requirements................................................................................................... 11 1.1.5 Dimensions...................................................................................................................... 11 1.1.6 Other Specifications......................................................................................................... 11 1.2 Specifications Allegra X-12R................................................................................................. 12 1.2.1 Speed.............................................................................................................................. 12 1.2.2 Time................................................................................................................................. 12 1.2.3 Temperature..................................................................................................................... 12 1.2.4 Electrical Requirements................................................................................................... 12 1.2.5 Dimensions...................................................................................................................... 12 1.2.6 Other Specifications......................................................................................................... 12 1.3 Allegra X-14............................................................................................................................. 13 1.3.1 Speed.............................................................................................................................. 13 1.3.2 Time................................................................................................................................. 13 1.3.3 Temperature..................................................................................................................... 13 1.3.4 Electrical Requirements................................................................................................... 13 1.3.5 Dimensions...................................................................................................................... 13 1.3.6 Other Specifications......................................................................................................... 13 1.4 Allegra X-14R.......................................................................................................................... 14 1.4.1 Speed.............................................................................................................................. 14 1.4.2 Time................................................................................................................................. 14 1.4.3 Temperature..................................................................................................................... 14 1.4.4 Electrical Requirements................................................................................................... 14 1.4.5 Dimensions...................................................................................................................... 14 1.4.6 Other Specifications......................................................................................................... 14 1.5 Allegra X-15R.......................................................................................................................... 15 1.5.1 Speed.............................................................................................................................. 15 1.5.2 Time................................................................................................................................. 15 1.5.3 Temperature..................................................................................................................... 15 1.5.4 Electrical Requirements................................................................................................... 15 1.5.5 Dimensions...................................................................................................................... 15 1.5.6 Other Specifications......................................................................................................... 15 1.6 General System Description.................................................................................................. 16 1.6.1 Centrifuge Function.......................................................................................................... 16 1.6.2 Available Rotors............................................................................................................... 17 1.7 Centrifuge Chassis................................................................................................................. 20 1.8 System Operating Specification............................................................................................21 1.9 Control and indicators........................................................................................................... 21 1.10 Electronics.............................................................................................................................. 22 1.10.1 Safety Features................................................................................................................ 23 2 INSTALLATION................................................................................................................................ 26 2.1 SAFETY NOTICE............................................................................................................................... 26 2.1.1 Electrical Safety............................................................................................................... 26 2.2 Allegra X-12/R, X-14/R, X-15R Installation Requirements...................................................28 2.2.1 Test Run........................................................................................................................... 28 2.3 Customer Training and Instrument Demo............................................................................30 2.3.1 Instrument Relocation...................................................................................................... 31 Rev 1.0 11/27/2019

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Allegra X-12/ R, Allegra X-14/R, Allegra X-15R, Service Manual Table of Contents 2.4 Customer Training Checklist.................................................................................................31 2.4.1 Rotor Handling and Maintenance Training.......................................................................31 2.4.2 Operator Training............................................................................................................. 31 3 SERVICE PROCEDURES................................................................................................................ 34 3.1 Mechanical Systems.............................................................................................................. 34 3.1.1 Front Panel Replacement................................................................................................34 3.1.6 Hinge or Door Replacement.............................................................................................38 3.1.7 Brake Resistor, EMI Filter or Receptacle Replacement...................................................39 3.1.8 Imbalance Adjustment and Calibration Allegra X-12 series..............................................39 3.1.2 Imbalance Calibration Allegra X-15R...............................................................................40 3.2 Temperature Control Systems...............................................................................................41 3.2.1 Refrigeration System Safety............................................................................................41 3.2.2 Refrigeration Functional Test...........................................................................................41 3.2.3 Checking the Refrigerant Charge.....................................................................................42 3.3 Troubleshooting................................................................................................................. 49 3.3.1 Component Resistance Checks........................................................................................49 3.2.4 Emergency Access.......................................................................................................... 53 3.3 Preventive Maintenance......................................................................................................... 54 3.3.1 Preliminary....................................................................................................................... 54 3.3.2 After Arrival...................................................................................................................... 54 3.3.3 Rotors.............................................................................................................................. 54 3.3.4 Drive................................................................................................................................ 54 3.3.5 Electrical and Functional Tests.........................................................................................55 4 ALLEGRA X-12/R, X-14, X-15R CIRCUIT DESCRIPTION..............................................................57 4.1 General.................................................................................................................................... 57 4.1.1 Control Driver Board........................................................................................................ 57 4.1.2 Motor Driver Board........................................................................................................... 59

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Allegra X-12/ R, Allegra X-14/R, Allegra x-15R Service Manual Revision History

Revision History Rev.

Date

Responsible

1.0

11/27/2019

Horst Krischer

Description

First release of new complete version of the manual.

Manual Use This Service Manual is copyrighted and all rights reserved by Beckman Coulter, Inc. This document may not in whole or part be copied, photocopied, reproduced, translated or reduced to any electronic medium or machine readable form without prior consent in writing from Beckman Coulter Inc. This Service Manual is designed solely for the use of Beckman Coulter Field Service Representatives who have training and experience in the servicing of Beckman Coulter products. To improve product performance, Beckman Coulter may have made changes in the product to which this Service Manual relates. Such changes may not be reflected in this manual. Accordingly, Beckman Coulter makes no representation or warranty, either express or implied, that the information contained in this Service Manual is complete or accurate with respect to the particular product to which this manual pertains, and the purchaser must assume all risks in the use of this manual for the purpose of performing service upon such product. Because of the possible hazards involved to an inexperienced person in servicing the product, Beckman Coulter recommends that servicing of the product be performed only by Beckman Coulter Field Service Representatives. Components of this instrument which are considered user serviceable are discussed in detail in the instrument's Instruction Manual.

Related Documents This Service Manual does not include the Instruction Manual for this instrument. The Instruction Manual contains instructions for proper operation of the instrument, information regarding the limitations, specifications, precautions and hazards associated with its use. To obtain a copy of the Instruction Manual, please order it through any Beckman Coulter Sales and Service Office. Part Number Description GX-IM-X Allegra X-12 Series Instruction Manual GX-IM-X-Allegra X-14 Series Instruction Manual GXR-IM-X Allegra X-15R Instruction Manual

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Safety Information Safety Reminders The following pages summarize cautionary information basic to the safe operation of this instrument. However, it is strongly recommended that the user read the entire manual carefully before attempting to service the instrument. In addition, be sure to heed all NOTES, CAUTIONS, and WARNINGS, which are specifically defined as follows:

NOTE: Used to make a procedure easier or more understandable. To disregard it may cause inconvenience, but not mechanical damage or personal injury.

CAUTION: Used to prevent equipment damage. To disregard the caution may cause mechanical damage however, personal injury is not likely.

WARNING: Used when an action or circumstance may potentially cause personal injury. Damage may also result.

WARNING: If the equipment is used in a manner not specified by Beckman Coulter, Inc., the protection provided by the equipment may be impaired. Electrical Safety To prevent electrically related injuries and property damage, properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies. Contact a service representative for any servicing of equipment requiring the removal of covers or panels. Avoid standing on damp floors. Disconnect the power cord before conducting any mechanical service on the instrument. Disconnect the power cord before working on high voltage circuitry .

High Voltage

This symbol indicates the potential of an electrical shock hazard existing from a high voltage source and that all safety instructions should be read and understood before proceeding with the installation, maintenance, and servicing of all modules. Turn the POWER off and disconnect the instrument from the main power source before performing any maintenance that requires the removal of an instrument panel.

Electrostatic Discharge Rev1.0 11/27/2019

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Check the grounding connections on the wrist strap and static mats. Ensure mat has proper ground. Touch conductive work surfaces before starting work. Do not allow anyone not grounded to touch ESD sensitive components. Do not touch the components by pins or leads. Boards should not be handled by anyone not properly grounded. Keep paper, non conductive plastic and plastic foams away from circuit boards and static free work areas. Keep hand creams and food away from conductive work surfaces. Take care when using soldering irons around conductive surfaces. Solder spills and heat from the gun may melt and damage the conductive mat. Mechanical Safety

Moving Parts To avoid injury due to moving parts, observe the following:  Under no circumstances attempt to slow or stop the rotor by hand.  Never attempt to exchange labware, rotors, or tools while the instrument is operating.  Never attempt to physically restrict any of the moving components of the Allegra X-12/R, Allegra X-14/R and Allegra X-15R.  Remember to keep clothing and fingers away from rotating or moving components. It is easy for tools and hands to slip or get cut while servicing the instrument if caution is not exercised.  Do not lean on the instrument during its operation.

Cleaning Observe any cleaning procedures outlined in the Instruction Manual for the Allegra X-12/R, X-14/R and Allegra X-15R. Prior to cleaning equipment that has been exposed to hazardous material:  Contact appropriate Chemical and Biological Safety personnel.  Review the Chemical and Biological Safety information contained in the Instruction Manual.

Maintenance Maintenance specified in this manual should be performed only by factory trained service representatives. After mechanical repairs are complete always ensure all mounting hardware is in place and that any safety shields or devices are installed.

Chemical and Biological Safety It is the user’s responsibility to have the components of the Allegra X-12/R, Allegra X-14/R and Allegra X-15R decontaminated before requesting service by a service representative or returning parts for repair. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. If any parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated.

Do not use or service the centrifuge in the vicinity of flammable liquids or vapors. Do not operate the instrument with flammable liquids in the rotor.

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Normal operation of the Allegra X-12/R, Allegra X-14/R and Allegra X-15R may involve the use of materials that are pathogenic, toxic, radioactive or otherwise biologically harmful. When such materials used, observe the following precautions:           

Contact the customer prior to any service to determine if any material used by the customer represents a toxic, pathological or radioactive hazard. Ensure the instrument is sterilized or decontaminated prior to service. Customer is responsible for any decontamination process. Ensure the proper personal protective equipment is used prior to servicing the instrument. Observe all cautionary information provided by the customer or laboratory safety officer. Dispose of all waste fluids (vacuum pump and diffusion pump oil) according to the facility’s waste disposal procedures. Operate the Allegra X-12/R, X-14/R, X-15R in accordance with the instructions outlined in the Instruction manual, and take all the necessary precautions when using pathological, toxic, or radioactive materials. Splashing of liquids may occur; therefore, take appropriate safety precautions, such as using safety glasses and wearing protective clothing, when working with potentially hazardous liquids. Use an appropriately contained environment when using hazardous materials. Observe the appropriate cautionary procedures as defined by a safety officer when using flammable solvents in or near a powered-up instrument. Observe the appropriate cautionary procedures as defined by a safety officer when using toxic, pathological, or radioactive materials.

NOTE: Observe all warnings and cautions listed for any external devices attached or used during operation of the X-12/R, Allegra X-14/R and X-15R.

CUSTOMER OPERATING INSTRUCTIONS The Allegra X-12/R, Allegra X-14/R and X-15R Instruction Manual is shipped as part of the instrument and is not included in this service manual. Additional copies of the "Allegra X-12/R, Allegra X-14/R and Allegra X-15R Centrifuge Instruction Manual" may be ordered from Technical Publications, 250 Kraemer Blvd., Brea, CA 92821 It is strongly recommended that the user read the entire instruction manual carefully before attempting to operate the instrument.

Waste Electrical and Electronic Equipment Directive Rev1.0 11/27/2019

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This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) directive of the European Union. The presence of this marking on the product indicates: The device is not to be disposed of via the municipal waste collection system of any member state of the European Union. It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.

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System Description Allegra X-12, Allegra X-12R, Allegra X-14, Allegra X-14R and Allegra X-15R are tabletop centrifuges designed for laboratory use. The Allegra X-12R, Allegra X-14R and Allegra X-15R are the refrigerated versions and the Allegra X-12 and Allegra X-14 are the constant Temperature versions. These laboratory centrifuges are microprocessor controlled. Complete operating instructions are found in the Users Manuals.

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1.1 Specifications Allegra X-12 1.1.1 Speed Set Speed Speed display Acceleration Deceleration

200 to 10200 rpm in 10-rpm increments Actual rotor speed in 10-rpm increments 10 acceleration profiles 11 deceleration rates

1.1.2 Time Set time Time Display Timed run Continuous (hold) or pulse run

To 99 hours 59 minutes or continuous (hold) Indicates run time remaining Indicates elapsed time

1.1.3 Temperature Set temperature Temperature control (after equilibration) Temperature display (after equilibration) Ambient temperature range Humidity restrictions

Factory set at 20°C + 2°C of the 20°C set temperature chamber temperature in 1°C increments 10 to 35°C