• Author / Uploaded
• 02joseluis02

Citation preview

&-A+11-6*%,(2+?+

!"#$%!"#$"%&!&'

Pipette into cuvettes

Enzymatic Colorimetric Test for Urea &'()'*+%,-.+/ 6 10505 !"#$% 10506 10507 10104 !&'(%

100 ml 1000 ml 1000 ml 9 x 3 ml

Sample/!0+(% Enzyme reagent 1a

Complete Test Kit Reagent 1, Enzyme, Standard Reagent 2 Standard

0+1234%1, 2, 3 Urea is hydrolysed in the presence of water and urease to produce ammonia and carbon dioxide. In a modified Berthelot reaction the ammonium ions react with hypochlorite and salicylate to form a green dye. The absorbance increase at 578 nm is proportional to the urea concentration in the sample. 5361+61/ !"#$%)) !"*+,% !"*+-% !#./% !0+(% !"*+,%

!"*+-%

!#./% !0+(%

7898: 1000 ml

7898;

1000 µl 1000 µl Mix, incubate for 10 min. at 20...25°C or for 5 min. at 37°C. Measure the absorbance of the sample (!A sample) and the !0+(% (!A !0+(%) against the reagent blank within 60 min. 5'C(EC'1-36%3F%!@+'%'64%G!H%536(+61@'1-36 !Asample C = """"" x factor !A!0+(% I'(13@

F3@%!@-6+M

"+'*+61%7 Phosphate buffer (pH 7.0) Sodium salicylate Sodium nitroprusside EDTA

9 x 3 ml 120 mmol/l 60 mmol/l 5 mmol/l 1 mmol/l

"+'*+61%= Phosphate buffer (pH < 13) 120 mmol/l Hypochlorite !"#$%"&'(")( Irritates eyes and skin. Keep out of reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor. #6.>?+ Urease > 500 KU/l ,1'64'@4 Urea equivalent to BUN Sodium azide

80 mg/dl or 13.3 mmol/l 37.28 mg/dl or 6.2 mmol/l 0.095 %

"+'*+61%&@+A'@'1-36 !"*+-%%and !0+(% are ready for use. The Enzyme reagent 1a is prepared by mixing the contents of bottle !#./% with bottle !"*+,%: !#./% + 100 ml !"*+,% or e.g. 1 ml 10 ml !#./% + 1000 ml !"*+,%

10 µl 1000 µl

!"*+-%

7878