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• StefenyAlegriia

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PhysioEx Lab Report Exercise 12: Serological Testing Activity 2: Comparing Samples with Ouchterlony Double Diffusion Name: Stefeny Zertuche Date: 30 October 2019 Session ID: session-59dea20b-8acf-e3b2-dc97-662be7283c63

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 When two antigens are identical their precipitin lines form You correctly answered: an arc. 2 Antigen and antibody move toward each other because of You correctly answered: diffusion. 3 If two antigens form a spur, they You correctly answered: have partial identity. 4 The Ouchterlony test relies on the formation of a(n) You correctly answered: precipitate.

Experiment Results Predict Question 1 Predict Question: How do you think human serum albumin and bovine serum albumin will compare? Your answer: They will have partial identity. Stop & Think Question 1 Which of the following best describes the two solutions you just added to well 1? You correctly answered: They are both antibodies produced by a goat. Experiment Data Wells

Identity

2 and 5

Partial

2 and 3

Identity

3

P ti l

d4

3 and 4

Partial

4 and 5

Identity

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 There is a spur between wells 2 and 5 and between wells 3 and 4 because the You correctly answered: antigens have partial identity. 2 What is the unknown antigen in well 5 (recall that well 2 contains bovine serum albumin and well 4 contains human serum albumin)? You correctly answered: human serum albumin. 3 Which of the following best describes this test? You correctly answered: Antibodies are used to detect the presence of antigens. 4 The antigen-antibody precipitate forms where the ratio of antigen and antibody is You correctly answered: optimum.

Review Sheet Results 1 Describe how you were able to determine what antigen is in the unknown well. Your answer: since human serum albumin was in well #4 and wells 4 and 5 were identical, this meant that the unknown well (#5) was human serum albumin as well.

2 Why does the precipitin line form? Your answer: Because antibodies and antigens diffuse toward each other, when the optimum ratio is present, a precipitate forms. 3 Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction? Your answer: Human serum albumin and bovine serum albumin did have partial identity because they had a single spur form. This indicated that they had epitopes in common.