Nikon Ti E service manual

R-4820.A Inverted Research Microscope EC LIPSE TI-E Repair Manual NIKON CORPORATION C 2004 NIKON CORPORATION All rig

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R-4820.A

Inverted Research Microscope

EC LIPSE TI-E

Repair Manual NIKON CORPORATION

C 2004 NIKON CORPORATION All rights reserved

R4820.A

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R4820.A

Contents Overview ...........................................................................................................................................P.1 Points on Repair ................................................................................................................................P.1 1.

Tools..................................................................................................................................................P.2 1-1.

Tools for General Use and Preparations ....................................................................................P.2

1-2.

Tools for Special Purposes .........................................................................................................P.3

2.

Specifications ....................................................................................................................................P.8 2-1.

3.

Specifications .............................................................................................................................P.8

Schematic Diagram of the Optical System ........................................................................................P.9 3-1.

Schematic Diagram of the Ti-E Optical System..........................................................................P.9

3-2.

Components in the Optical System ..........................................................................................P.10

4.

Connection Diagram........................................................................................................................ P.11 4-1.

5.

Inner Connection Diagram of Ti-E ............................................................................................ P.11

Removing/Installing the Optical Unit................................................................................................P.12 5-1.

Removing the Accessories .......................................................................................................P.12

5-2.

Removing the Optical Unit........................................................................................................P.13

5-3.

Installing the Optical Unit..........................................................................................................P.17

6.

Disassembling/Assembling the Up-Down Movement Unit...............................................................P.18 6-1.

Disassembly.............................................................................................................................P.18

6-2.

Assembly..................................................................................................................................P.26

7.

Disassembling/Assembling the Joint Gears ....................................................................................P.29 7-1.

Disassembly.............................................................................................................................P.30

7-2.

Assembly..................................................................................................................................P.35

8.

Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob ..................................................P.36 8-1.

Disassembling the Coaxial Coarse/Fine Focus Knob ..............................................................P.37

8-2.

Assembling the Coaxial Coarse/Fine Focus Knob ...................................................................P.43

9.

Disassembling/Assembling the Second Objective Lens ..................................................................P.44 9-1.

Disassembling the Second Objective Lens ..............................................................................P.45

9-2.

Assembling the Second Objective Lens ...................................................................................P.47

10.

Disassembling/Assembling the Optical Path Change-Over Block ...................................................P.49

10-1.

Disassembling the Optical Path Change-Over Block ...............................................................P.50

10-2.

Assembling the Optical Path Change-Over Block ....................................................................P.52

R4820.A

11.

Optical Adjustment ..........................................................................................................................P.53

11-1.

Preparation for Optical Adjustment...........................................................................................P.53

11-2.

Adjustment of Stop Position of Turret .......................................................................................P.57

11-3.

L100 (Prism in Left Observation Optical System) Optical Axis Adjustment ..............................P.59

11-4.

Focusing and Optical Axis Adjustment on the Objective Side ..................................................P.60

11-4-1. Preparation............................................................................................................................P.60 11-4-2. Adjustment of the Optical Axis and Parfocality of the 1x Second Objective Lens..................P.62 11-4-3. Adjustment of the Optical Axis and Parfocality of the 1.5x Second Objective Lens...............P.63 11-5.

Preparations Common to Overall Optical Adjustments.............................................................P.64

11-5-1. Setting...................................................................................................................................P.64 11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment...............................................P.65 11-5-3. Focusing ...............................................................................................................................P.66 12.

Overall Optical Adjustment ..............................................................................................................P.68

12-1.

Focusing and Optical Axis Adjustment on the Observation System .........................................P.68

12-1-1. Preparation ...........................................................................................................................P.68 12-1-2. Focusing and Optical Axis Adjustment ..................................................................................P.69 12-1-3. Beam Adjustment..................................................................................................................P.70 12-2.

Focusing and Optical Axis Adjustment on the Side Port...........................................................P.71

12-2-1. Preparation (for focusing and optical axis adjustment on the right- and left-side ports) ........P.71 12-2-2. Optical Axis Adjustment ........................................................................................................P.72 12-2-3. Focusing Adjustment.............................................................................................................P.73 13.

Electrical Adjustments .....................................................................................................................P.75

13-1.

Encoder Adjustment .................................................................................................................P.75

13-1-1. Preparation ...........................................................................................................................P.75 13-1-2. Adjustment of Tilt of Encoder Head.......................................................................................P.76 13-1-3. Amplitude (Gain) Adjustment of the Preamplifier Board ........................................................P.78 13-1-4. Center Level (Offset) Adjustment of the Preamplifier Board .................................................P.79 13-1-5. Confirmation of Lissajous Waveform ....................................................................................P.80 13-1-6. Signal Specifications .............................................................................................................P.81 13-2.

Upper- and Lower-Limit Sensor Adjustment.............................................................................P.82

13-2-1. Checking Limit Sensor Operation .........................................................................................P.82 13-2-2. Limit Sensor Mounting Position Adjustment..........................................................................P.82 13-3.

Adjustment of the Position Sensor in the Optical Path Change-Over Turret ............................P.83

13-3-1. Adjustment of the Stop Position Sensor ................................................................................P.83 13-3-2. Adjustment of the Zero Position Detection Sensor................................................................P.84

R4820.A

Appendix: Test Mode................................................................................................................................P.85 1.

Test Mode Hierarchy ................................................................................................................P.85

2.

Displaying Information About the Main Body System...............................................................P.87

3.

2-1.

Page 1 -- Checking switch inputs........................................................................................P.88

2-2.

Page 2 -- Reading limit sensors ..........................................................................................P.89

2-3.

Page 3 -- Checking set signals ...........................................................................................P.89

2-4.

Page 4 -- Checking the piezoelectric focus drive ................................................................P.90

2-5.

Page 5 -- PFS nosepiece information .................................................................................P.90

2-6.

Page 6 -- Z motor drive and optical-path change motor drive .............................................P.91

2-7.

Page 7 -- Bottom and offset lens motor drives ....................................................................P.91

Displaying Information About the Hub System .........................................................................P.92 3-1.

Page 1 -- Motorized accessories.........................................................................................P.95

3-2.

Page 2 -- Micrometer ..........................................................................................................P.95

3-3.

Page 3 -- Stage ...................................................................................................................P.96

3-4.

Page 4 -- Shutter.................................................................................................................P.96

3-5.

Page 5 -- DIA illumination ...................................................................................................P.97

3-6.

Page 6 -- Intensilight ...........................................................................................................P.97

3-7.

Page 7 -- DSC ....................................................................................................................P.98

3-8.

Page 8 -- Handle and knob data .........................................................................................P.98

3-9.

Page 9 -- PFS nosepiece PID.............................................................................................P.99

3-10. Page 10 -- Hub-side nosepiece PID....................................................................................P.99 3-11. Page 11 -- Ergonomic controller and joystick information .................................................P.100 4.

5.

Displaying/Editing PFS Parameters .......................................................................................P.101 4-1.

Page 1 -- Speed control parameters .................................................................................P.102

4-2.

Page 2 -- Control speed gain ............................................................................................P.102

4-3.

Page 3 -- Automatic adjustment division boundary line.....................................................P.103

4-4.

Page 4 -- Offset zero-position setting................................................................................P.104

Displaying/Editing Software DIP Switches .............................................................................P.105 5-1.

6.

Page 1 -- Speed control parameters .................................................................................P.106

Displaying/Editing PFS Parameters (for supporting sold products) ........................................P.107 6-1.

Page 1 ..............................................................................................................................P.108

Overview

R-4820.A

Overview 1. This repair manual covers Ti-E model. 2. This repair manual has been prepared as a textbook for the repair training session. It provides step-by-step instructions that are designed to enable service personnel to gain an understanding of the structure of the mechanical and optical systems, and to learn how to repair them. 3. When you conduct repair work, be very careful of any sharp edges, projecting object, and conductive parts to avoid injury or electric shock. (It is recommended that you unplug the power cable and wear safety gloves while you are conducting repair work.)

Points on Repair ○ ○ ○

Use a Phillips screwdriver that appropriately fits the slot in a screw head used. Clean plastic parts with mild detergent. Ether, thinner, alcohol and other organic solvents may cause discoloration or deterioration. To clean the glass parts, first blow the dust off them and then wipe them using lens tissue moistened with alcohol (or alcohol-ether mixture).

-1-

1. Tools

R-4820.A

1-1. Tools for General Use and Preparations

Tool No J21084 T91400 T91000 T91204 T91102 T91305

T91320

Name Hex-key set Phillips screwdriver set Slotted screwdriver set Phillips screwdriver w/wood handle Slotted screwdriver w/wood handle Phillips screwdriver bit (For JCIS screw M2, M2.6)

Illustration Set (x 6) Set (x 4) Set (x 6) 0.236 in. in diameter 0.217 in. width 0.098 in. φ2.5mm 4.528 in. φ115mm

Phillips screwdriver bit holder

T92041

Tweezers AA

T92162

Compass spanner A

T92163

Compass spanner B

E719

Handlap (Alcohol container)

90mm 3.543 in. φ8mm 0.315 in.

Alcohol (or alcohol-ether mixture), lens tissue, blower, gasoline (for cleaning), bamboo stick (for applying lubricant and adhesive), dial gauge, scraper Lubricant and Adhesive: Refer to Parts Lists (KR-7959).

-2-

1. Tools

R-4820.A

1-2. Tools for Special Purposes J25417 Tool legs (four in a set; common to TE2000) Note: To order, fill in the quantity as four, not as one set.

J25578 Ti Optical path adjustment mount 2 New

J25433 Reference revolver for round dovetail (common to TE2000)

J25425 Reference straight tube (for objective) (f200)

-3-

1. Tools

R-4820.A

1-2. Tools for Special Purposes J25575 Ti Reference straight tube (for body tube) (f210) New

J25438 Alignment clamp screw (common to TE2000) Set (x 2) Note: To order, fill in the quantity as two.

J25572B Ti Output port straight tube B (V-T straight tube) New

J25572A Ti Output port straight tube A New

-4-

1. Tools

R-4820.A

1-2. Tools for Special Purposes J25573A,B Ti Reference reticle

J15573A

J25574 Ti Tool body tube base

J25220 Crosshair eyepiece

J25221 Tool eyepiece

-5-

J15573B

1. Tools

R-4820.A

1-2. Special Purposes 1-2.Tools Toolsfor for Special Purposes

J29102 Dioptric telescope

J28005 1-minute reading collimator

J28171 Tool for adjusting infinite optical path difference

J28003 Parallel mirror

-6-

1. Tools

R-4820.A

1-2. Tools for Special Purposes

J28004 Grid sample

J25194 Dark crosshair sample

J25172 Centering scope

0.913 in.

-7-

2. Specifications

R-4820.A

2. Specifications 1.

Name Ti-E INVERTED MICROSCOPE

2.

Observation Optical System Three-time reflection, Erecting observation, Field number: 22

3.

Output Optical System Side port: CCD camera, BA filter wheel, Confocal head, Adapter straight tube for the photometric sensor (Attachable and removable) Observation port: Used with Ti-T-BPH EYEPIECE TUBE BASE UNIT WITH PH or Ti-T-BS EYEPIECE TUBE BASE UNIT WITH SIDE PORT

4.

Optical Path Selection Light Intensity Distribution EYE: Observation 100%, L100: Left side 100%, R100: Right side 100% L80: Observation 20% Left side 80% Note: R100 and L80 each are replaceable with an option prism. Motor: Two-phase pulse motor Change-over time: 1 sec or less (between adjacent positions)

5.

Up-Down Movement Unit Stroke: From the reference position, up: 6.57mm or higher, down: 2.03mm or higher 10±0.2mm overall Motor: Five-phase pulse motor Operation Speed: Three types (Ex Fine/Fine/Coarse) Maximum speed: 2.5mm/sec (Reference value) Minimum step: 0.025± 0.025μm Refocus system: Using Escape/Refocus switch Escape is located 2mm below the reference position.

6.

Others As requested by a person in charge, other specifications will be added with the approval of Nikon.

-8-

3. Schematic Diagram of the Optical System

R-4820.A

3. Schematic Diagram of the Optical System 3-1. Schematic Diagram of the Ti-E Optical System

Second objective lens

Optical path change-over prism Fig. 3-1

-9-

3. Schematic Diagram of the Optical System

R-4820.A

3. Schematic Diagram of the Optical System 3-2. Components in the Optical System

Specimen focus plane

Optical axis of observation system

Objective mounting position

Left-side port optical axis

Second objective lens Optical path change-over prism

Right-side port optical axis Primary image plane

Fig. 3-2

- 10 -

4. Connection Diagram

R-4820.A

4. Connection Diagram 4-1. Inner Connection Diagram of Ti-E

- 11 -

5. Removing/Installing the Optical Unit

R-4820.A

5-1. Removing the Accessories Remove the accessories that make up the microscope to prevent them from obstructing the disassembling, assembling and optical system adjustments of the internal components of the microscope.

5-1. Removing the Accessories 1. Stage Set the movable part of the stage to the center of the movable range in the X and Y directions each and remove the stage.

2. Revolver Remove the revolver with the objective lens attached.

Stage securing bolts

Revolver securing bolts 3. Light post The center of gravity is located in the upper portion of the light post. Remove it being careful it does not to fall over.

4. Body tube Loosen the body tube clamp screw and remove the body tube upward from the body tube adapter.

Post securing bolt Body tube clamp screw

5. Body tube adapter Remove the body tube adapter from the microscope base.

Adapter securing bolts

- 12 -

5. Removing/Installing the Optical Unit

R-4820.A

5-2. Removing the Optical Unit 5-2. Removing the Optical Unit 1.

Remove the button bolts (#0202x2, #0222x2) from the rear panel. (Fig. 5-1)

Fig. 5-1 2.

Lay the microscope base with its rear side down and remove the hexagonal button bolts (#0202x8) to detach the bottom plate. (Fig. 5-2)

Fig. 5-2 3.

Remove the flat cable (#0814). Step: Disconnect the stopper from J5 connector and the same from the cable retainer on the relay board (#0804). (Fig. 5-3)

Fig. 5-3

- 13 -

5. Removing/Installing the Optical Unit

R-4820.A

5-2. 5-2. Removing Removing the the Optical Optical Unit Unit 4.

Remove the hexagonal bolts with washers (#0108x4) and detach guide plate (#0105). (Fig. 5-4)

Hexagonal bolts

Fig. 5-4 5.

Disconnect four connectors (J3, J4, J6, and J8) from the CPU/memory board (#0801). Disconnect two connectors (J1 and J6) from the interface board (#0802). (Fig. 5-5)

J8 J3 J1

J6

J6

J4

Fig. 5-5 6.

Remove the four hexagonal bolts (#203x4) securing the interface board (#0802) and pull out the board. (Fig. 5-6)

Bolts securing the board

Fig. 5-6

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5. Removing/Installing the Optical Unit

R-4820.A

5-2. Removing the Optical Unit 7.

Disconnect the connector (Fig. 5-7) from the encoder cable (#0824) and disconnect the connector (Fig. 5-8) from the laser safety switch cable (#0684).

Fig. 5-7

8.

Fig. 5-8

Loosen the setscrews (#0653x2) securing the second objective lens selector joint. Move the loosened joint connection in the direction indicated by the arrow. (Fig. 5-9)

Setscrew #0653

Fig. 5-9

9.

Remove the optical unit from the microscope base. (#0203x6) (Fig. 5-10)

[Notes] • When removing the bolts, securely hold the optical unit with your hand to prevent it from falling. • When removing the optical unit, exercise extreme care not to let the unit components bang against the surroundings.

Fig. 5-10

- 15 -

5. Removing/Installing the Optical Unit

R-4820.A

5-2. Removing the Optical Unit 10. Attach legs (J25417) to the optical unit. (Fig. 5-11) Store the optical unit to protect it from dirt and dust.

Fig. 5-11 Now the removal of the optical unit is complete.

- 16 -

5. Removing/Installing the Optical Unit

R-4820.A

5-3. Installing the Optical Unit 5-3. Installing the Optical Unit 1. Lay the microscope base with its rear side down. 2. Set the secondary objective lens to the 1x position. 3. Detach the four legs from the optical unit. (Refer to Fig. 5-11.) 4. Put the optical unit into the microscope base while securely holding the unit with your hand. [Notes] • Do not get two harnesses (one connected to the precise encoder and the other connected to the interlock) inside the microscope front caught between the optical unit and the microscope base. • When installing the optical unit, exercise extreme care not to let the unit components bang against any other components. 5. Secure the optical unit while pushing it against the reference mounting plane (left and rear: Refer to Reference Fig. 5-12.) of the microscope base. (#0203x6) planes

Fig. 5-12

6.

7.

8. 9. 10. 11. 12. 13.

Hold the joint against and secure it with two setscrews (#0653x2). (Refer to Fig. 5-9.) Make sure that you can hear a "click" sound when you change the second objective lens position from 1x to 1.5x. Connect the two harness connectors to each of the precision encoder and interlock inside the microscope base. (Refer to Figs. 5-7 and 5-8.) Secure the interface board with the four hexagonal bolts (#023x4). (Refer to Fig. 5-6.) Connect the four connectors to the CPU and memory board and connect the two connectors to the interface board. (Refer to Fig. 5-5.) Secure the guide plate (#0105) with the hexagonal bolts with washers (#0108x4). (Refer to Fig. 5-4.) Attach the flat cable (#0814). (Refer to Fig. 5-3.) Secure the flat cable using the stopper of the cable retainer. Mount the bottom plate with the hexagonal button bolts (#0202x8). (Refer to Fig. 5-2.) Bring the microscope back to horizontal.

- 17 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit Note: Hereinafter, some illustrations are copied from MEA53100 repair parts list KR-7959.

6-1. Disassembly

- 18 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit • Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1. 2.

Remove the optical unit. (Refer to 5-2. Removing the Optical Unit.) Disconnect the connector and harness. (Fig. 6-1)

Connector 3. 4.

Bring the microscope base back to horizontal. Remove the button head screws (#0202x2) securing the protective cover (#0118) and detach it. Then, slide the sledge (#0130) and remove it. (Fig. 6-2)

Harness

Fig. 6-1

Button head screw

Fig. 6-2

Fig. 6-2A

- 19 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit 5.

Remove the up-down movement cover (#0418). (Fig. 6-3) (Hexagonal button bolts #0457x4)

Fig. 6-3 Fig. 6-3A

6.

Lift the up-down movement unit in the direction of the arrow to detach it from the microscope base. (Fig. 6-4) (Hexagonal bolts with washers #0204x4)

7.

Detach upper/lower limit sensor board (#0705). (Fig. 6-5) (Hexagonal bolts #0453x2)

Fig. 6-4 Limit sensor board #0705

Hexagonal bolts #0453

Fig. 6-5 Fig. 6-5A

- 20 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit 8. Loosen the setscrew (#0462x1) and pull out the linear encoder mirror mounting frame (#0409) to the right in the picture. (Fig. 6-6)

Setscrew #0462

Mirror mounting frame #0409

Fig. 6-6

Fig. 6-6A

9.

Detach the index of the linear encoder from the encoder support frame (#0416). (Fig. 6-8) (Hexagonal bolts #0451x2) Note: Handle the index with care to prevent disconnection or deformation of a leaf spring.

Bolt securing the index #0451

Fig. 6-7

Fig. 6-8

Fig. 6-7A

- 21 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit 10. Loosen the bolts through the openings of #0416 and pull the glass scale of the linear encoder out from #0417 to the left in the picture (Fig. 6-9). (Figs. 6-9, 6-9A and 6-10) (Hexagonal bolts #0452x2) When pulling the glass scale out, exercise care so as not to hit it against surrounding parts. Bolt securing the scale #0452

Fig. 6-9

Fig. 6-10

Fig. 6-9A

- 22 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit 11. Detach the encoder preamplifier board from #0416. (Fig. 6-11) (Hexagonal bolts #0455x3)

Fig. 6-11

Fig. 6-11A

12. Remove the up-down movement stroke limiting screw through #0416. (Fig. 6-12) (Hexagonal bolt with washer #0453x1)

Fig. 6-12

13. Loosen the roller race adjusting screws (#0463x3) on #0416. (Fig. 6-13)

Fig. 6-13

- 23 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit 14. Aim the protruding part of the encoder downward and remove the captive screw for the cylindrical roller bearing (#0454) on the opposite side (Fig. 6-15) to the rack. (One location only) (Fig. 6-14)

Fig. 6-14 15. Remove the hexagonal bolts with washers (#0456x3) securing the roller race (#0407). (Fig. 6-15)

Fig. 6-15 16. Slide the roller race (#0407) and remove it. (Fig. 6-16)

Fig. 6-16

- 24 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-1. Disassembling the Up-Down Movement Unit 17. Remove the race support (#0417) at the center from the up-down movement base. (Fig. 6-17) When you remove the race pad, cylindrical roller bearing (#0402) comes out and the rollers get scattered. Exercise care so as not to lose them.

Fig. 6-17

Fig. 6-17A

Notes • Remove the rack (#0406) if necessary. • Replace the cylindrical roller bearing (#0402) and retainer (#0411) if rust, dent or wear is found. • Remove rust, if any exists, from the roller race mounting surface with vinyl adhesive and fine sharpening stone. Now, the up-down movement block has been disassembled. Clean the components.

- 25 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-2. Assembling the Up-Down Movement Unit 6-2. Assembly 1. Place the retainer on the roller race secured to the up-down movement base and alternately put the fourteen cylindrical rollers with lubricant (L4010) applied. (Fig. 6-18)

Fig. 6-18 2.

Place the retainer on the roller race secured to the race support and alternately put the fourteen cylindrical rollers with lubricant (L4010) applied. (Fig. 6-19)

Fig. 6-19 3. Fit the race support into the up-down movement base. 4. Slide the roller race to fit to the unit and secure the race temporarily with the hexagonal bolts (#0456x3). 5. Attach the captive screw for the cylindrical roller bearing (#0454, #0464) you removed in Step 6-1-14 to the roller race and apply adhesive (#757) to the screw. 6. When you place the retainer on the roller race, adjust the position of the retainer so that the center of its movable part will align with that of the movable part of the up-down movement unit. 7. Move the race support of the movable parts to the center, tighten the adjusting screws (#0463x3) (Fig. 6-13) and push the roller race you temporarily secured in Step 6-2-4 against the movable race support. 8. When you move the race support, the cylindrical roller bearing and retainer should smoothly move. Make sure that the race support of the movable parts smoothly moves and no uneven or rough movements exist. Using a spring scale, push the movable part and ensure that the initial resistance when it starts moving is in the range from 100 to 150 g. If the initial resistance is outside the above range, follow Step 6-2-7 to adjust it using the adjusting screw. Then, tighten the bolts (#0456x3) you temporarily secured in Step 6-2-4. * Tightening the bolts (#0456x3) may adversely affect the movement of the race support of the movable parts. If that happens, repeat Step 6-2-7. 9. Apply adhesive (#757) to the threads of the adjusting screws (#0463x3) you tightened in Step 6-2-7.

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6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-2. Assembling the Up-Down Movement Unit 10. Fit the up-down movement stroke limiting screw (hexagonal bolt with washer #0453x1). 11. Attach the encoder preamplifier board you removed in Step 6-1-11. 12. 13. 14. 15. 16. 17.

Attach the scale you removed in Step 6-1-10 to the encoder. Attach the index you removed in Step 6-1-9 to the encoder. Attach the mirror mounting frame you removed in Step 6-1-8. Adjust the encoder following the procedure described in Step 13-1. Encoder Adjustment. Attach the upper/lower limit sensor board you removed in Step 6-1-7. Fit the up-down movement unit into the microscope base, push it against the reference planes (Fig. 6-20) in the back of the body and on the left side in the direction of the arrow (Fig. 6-21). (Hexagonal bolts with washers #0204x4)

Reference planes

Fig. 6-21

Fig. 6-20

Reference planes

Fig. 4-20A

- 27 -

6. Disassembling/Assembling the Up-Down Movement Unit

R-4820.A

6-2. Assembling the Up-Down Movement Unit 18. Adjust the position where the limit sensor board is installed following the procedure described in Step 13-2. Upper- and Lower-Limit Sensor Adjustment. 19. Attach the up-down movement cover (#0418). (Hexagonal button bolts #0457x4) Now, the up-down movement unit has been assembled.

- 28 -

7. Disassembling/Assembling the Joint Gears

7-1. Disassembling the Joint Gears

- 29 -

R-4820.A

7. Disassembling/Assembling the Joint Gears

R-4820.A

7-1. Disassembling the Joint Gears 7-1. Disassembly • Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1. 2. 3.

Remove the optical unit. (Refer to 5-2. Removing the Optical Unit.) Bring the microscope base back to horizontal. Remove the protective cover (#0118). Slide the dummy sledge (#0130) in the direction indicated by the arrow and remove it. (Hexagonal button bolts #0202x2) (Fig. 7-1)

Fig. 7-1

Fig. 7-1A 4.

Remove the cover (#0115) from the microscope base (#0101). (Hexagonal button bolts #0202x2) (Fig. 7-2)

Bolts securing the cover #0202

Fig. 7-2

5.

6. 7.

Remove the up-down movement unit from the microscope base. (Refer to 5-2. Removing the Optical Unit.) Lay the microscope base with its rear side down. Disconnect the pulse motor connector. (Fig. 7-3)

Connector of pulse motor

Fig. 7-3

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7. Disassembling/Assembling the Joint Gears

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7-1. Disassembling the Joint Gears 8.

Detach the joint while holding it from inside. (Hexagonal bolts with washers #0202x4) Inside the base

Joint

Fig. 7-5

Fig. 7-4

Fig. 7-5A

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7. Disassembling/Assembling the Joint Gears

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7-1. Disassembling the Joint Gears 9.

Loosen the two setscrews (#0377x2) and remove the large reduction gear. (Fig. 7-6)

Reduction gear #0355

Setscrews #0377

Fig. 7-6A

Fig. 7-6 Fixing screws #0373

10. Detach the pulse motor (#0828). (Screws #0373x4) (Fig. 7-7)

Fig. 7-7 11. Loosen the setscrew (#0377x1) and detach the pulley (#0356). (Figs. 7-8 and 7-9) Replace the pulse motor if necessary.

Setscrew #0377

Fig. 7-8

- 32 -

Fig. 7-9

7. Disassembling/Assembling the Joint Gears

R-4820.A

7-1. Disassembling the Joint Gears

Fig. 7-7, 8, 9A

Do the following if the reduction gear is required to be disassembled. 12. Remove the pan head screws (x6) and detach the bearing (#360). (Fig. 7-10)

Fixing screws

Bearing

Fig. 7-10 13. Remove five ball bearings (#0353x5) on the bearing (#0360). 14. Remove gears (#0351, #0352, #0354, #357, #0358) and ball bearings (#0353x5) from the shaft support. (Fig. 7-11)

Fig. 7-11

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7. Disassembling/Assembling the Joint Gears

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7-1. Disassembling the Joint Gears

Fig. 7-10, 11A

Now, the joint gears have been disassembled. Check the ten ball bearings for movement. If there is inconsistent movement or galling, replace them.

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7. Disassembling/Assembling the Joint Gears

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7-2. Assembling the Joint Gears 7-2. Assembly 1. Fit the ball bearings (#0353x5) into the shaft support. 2. Apply lubricant (G9110) to the teeth of each gear and fit the gears into the ball bearing in the shaft support. (Refer to Fig. 7-11.) 3. Reinstall the ball bearings (#0353x5) to each gear shaft. 4. Reinstall the bearing and fit the ball bearing into the hole in the bearing (#0360). 5. Secure the bearing with the screws with washer (#0375x6). (Position the bearing using the pin #0378.) 6. Apply adhesive (#757) to the specified screws. 7. Attach the pulley (#0356) to the pulse motor (#0828). • Secure the screws securing the pulley on the D-Cut surface of the shaft of the pulse motor. 8. Reinstall the pulse motor to the joint with the four screws. 9. Reinstall the reduction gear (#0355). 10. Put the joint into the microscope base. Secure the joint using the hexagonal bolts with washer (#0202x4) in the top of the microscope base while pressing the joint against the reference plane (toward upper left in the picture). (Fig. 7-12)

Fig. 7-12 Reference planes

Fig. 7-13

11. Secure the up-down movement unit with the hexagonal bolts while pressing it against the reference plane of the microscope base. (Refer to 6-1. Disassembling the Up-Down Movement Unit.) 12. Attach the optical unit while pressing it against the reference plane. (Refer to 5-3. Installing the Optical Unit.) 13. Bring the microscope base back to horizontal and secure the protective cover with the two hexagonal button bolts. 14. Attach the cover. 15. Attach the dummy sledge. Now, the joint has been assembled.

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8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-1. Disassembling the Coaxial Coarse/Fine Focus Knob

- 36 -

8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 1. 2.

Remove the optical unit. (Refer to 5-2. Removing the Optical Unit.) Using an appropriate tool such as chopsticks, remove rubber cap (#0313) from each of the left and right fine control handles. (Fig. 8-1)

3.

Remove fine control knob (#0302) on the right and left sides. • Remove the screws (two each for right and left side) that secure the fine control knobs on both sides. (Fig. 8-2)

Fig. 8-1

Fig. 8-1A

Setscrews

Fig. 8-2A

Fig. 8-2

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8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 4.

Remove left decorative ring (#0309) on the left side. (Fig. 8-3) 1) Loosen the setscrews (#0377x3) that lock retaining ring (#0305) to remove the ring. 2) Remove the decorative ring. Hexagonal bolts (#0371x2)

Hexagonal bolts

Locking setscrews

Fig. 8-3

Fig. 8-3A 5. 6.

Make the microscope upright with its rear side down. Remove right decorative ring (#0310) on the right side. (Figs. 8-4 and 8-5) 1) Remove the inner hexagonal bolts (#0372x2). Note: Be sure to hold the handle unit inside to prevent it from falling.

Hexagonal bolts

Fig. 8-4

Fig. 8-4A Handle unit

Fig. 8-5 - 38 -

8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 7.

Slide the handle in the direction of the arrow to remove it from the microscope base. (Fig. 8-6)

Fig. 8-5A Fig. 8-6 8. Loosen the screws (#0376x2) fixing thrust retaining ring (#0306) to remove it. (Fig. 8-7)

Thrust retaining ring #306

Setscrews #376 9.

Remove Delrin washers (#0301) and corrugated washer (#0303). (Fig. 8-8) Note: Be sure to remove the two Delrin washers.

Fig. 8-7

Fig. 8-8 Fig. 8-8A

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8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 10. Remove the hexagonal bolts (#0372x2) to detach the encoder cover (#0312). (Figs. 8-9 and 8-10) Note 1: Pay attention to Delrin washer (#0301) between the encoder cover and encoder scale (#0826). 2: The encoder scale tends to bend, so handle it with care. Cover #0312

Encoder scale #0826

Hexagonal bolts #0372

Fig. 8-10

Fig. 8-9 11. Remove the encoder head (#0824). 1) Remove the two screws (#0375) fixing the encoder head. (Fig. 8-11)

Screws fixing the encoder #0375

Fig. 8-11 12. Loosen the screws (#0376x2) fixing the thrust retaining ring (#0308) and remove it. (Fig. 8-12)

Fixing screws #0376

Thrust retaining ring #0308

Fig. 8-12

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8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 13. Loosen the screws (x2) fixing encoder scale (#0826) and detach the scale from the fine motion shaft. (Fig. 8-13)

Screws fixing the scale

Encoder scale #0826 Fig. 8-13

Fig. 8-9, 10, 11, 12, 13

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8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 14. Loosen the screws (#0376x2) fixing the thrust retaining ring (#0306) and remove it from the handle shaft (#0304). (Fig. 8-14)

Thrust retaining ring #0306

Fixing screws #0376

Fig. 8-14

Fig. 8-14A

15. Loosen the screws (#0376x2) fixing the thrust retaining ring (#0308) and remove it from the handle shaft (#0304). (Fig. 8-15)

Thrust retaining ring #0308

Fine motion shaft #0304

Fixing screws #0376

Fig. 8-15

Replace the encoder scale if it is bent or damaged, if necessary. Replace the fine motion shaft if it is bent or damaged, if necessary. Wipe the oil off of the oil holes after disassembling.

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8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob

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8-2. Assembling the Coaxial Coarse/Fine Focus Knob 8-2. Assembling the Coaxial Coarse/Fine Focus Knob 1. Fit the thrust retaining ring so as to allow the setscrew to seat in the V groove of the fine motion shaft. (Refer to Fig. 8-15.) 2.

After applying oil to the encoder holder (#0311), insert the fine motion shaft and fit the thrust retaining ring. (Refer to Fig. 8-14.)

3.

Secure the encoder scale to the fine motion shaft with two fixing screws. (Refer to Fig. 8-13.)

4.

Secure the thrust retaining ring to the fine motion shaft with two fixing screws. (Refer to Fig. 8-12.)

5.

Install the encoder head with two screws. (Refer to Fig. 8-11.) Check that the encoder head does not come into contact with the scale.

6.

Fit the Delrin washers and secure the encoder cover with the two hexagonal bolts. (Refer to Figs. 8-9 and 8-10.)

7.

Insert the Delrin washer, corrugated washer and another Delrin washer in that order and secure the thrust retaining ring with the two screws. (Refer to Fig. 8-8.)

8.

Fit the handle to the microscope and secure the right decorative ring with the two hexagonal bolts.

9.

Secure the left decorative ring with the four hexagonal bolts.

10. Attach the retaining ring and secure it with the three locking screws. 11. Secure the right and left fine motion handles with screws, two each side. 12. Attach the rubber cap to each of the fine control handle.

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9. Disassembling/Assembling the Second Objective Lens

9-1. Disassembling the Second Objective Lens

- 44 -

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9. Disassembling/Assembling the Second Objective Lens

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9-1. Disassembling the Second Objective Lens 9-1. Disassembling the Second Objective Lens • Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1.

2.

Remove the optical unit from the microscope base. (Refer to 5-2. Removing the Optical Unit.) Loosen the setscrews (#0654x2) in the fulcrum (center of rotation) of the magnification variable turret (#0502) and pull the unit out of #0518 upward. (Fig. 9-1)

Setscrews #0654x2

Fig. 9-1 3.

Detach 1x lens cell holder (#0503) and 1.5x lens cell holder (#0507) from the magnification variable turret. (Figs. 9-2 and 9-3) (Hexagonal bolts with washers #0669x2 each)

1.5x lens cell

1x lens cell

Securing bolts #0669

Fig. 9-2

Securing bolts #0669 Setscrew #0653

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Fig. 9-3

9. Disassembling/Assembling the Second Objective Lens

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9-1. Disassembling the Second Objective Lens 4. 5.

Loosen the setscrew (#0653x2) in the 1x lens cell holder. Rotate the lens cell (#0504) counterclockwise and remove it. Rotate the retaining ring (#0506) counterclockwise and detach it from the lens cell. This causes lens (#L001), separation ring (#0505) and lens (#L002) to detach. Particular care must be exercised so as not to damage the lens in the lens cell.

Optical diagram of 1x lens cell

Fig. 9-5

Fig. 9-4

6. Loosen the setscrew (#0653x2) in the 1.5x lens cell holder. Rotate the lens cell (#0508) counterclockwise and remove it. 7. Rotate the retaining ring (#0510) counterclockwise and detach it from the lens cell. This causes lens (#0016), separation ring (#0509) and lens (#L007) to detach. Particular care must be exercised so as not to damage the lens in the lens cell. 8. Rotate the retaining ring (#0511) counterclockwise and detach it from the lens cell. This causes lens (#L008) to detach. Particular care must be exercised so as not to damage the lens in the lens cell.

Optical diagram of 1.5x lens cell

Fig. 9-6

Fig. 9-7

Now, the second object turret unit has been disassembled. Note: Using lens paper, clean the removed lenses. Wash hardware components.

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9. Disassembling/Assembling the Second Objective Lens

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9-2. Assembling the Second Objective Lens 9-2. Assembling the Second Objective Lens 1.

Mount the lens (#L008) to the 1.5x lens cell (#0508) as shown in Fig. 9-7, and then screw the retaining ring (#0511) down tight. Apply adhesive (#757) to the threads of the retaining ring.

2.

Mount the lens (#L007) to the same lens cell as shown in Fig. 9-7, and fit the separation ring (#0509). Mount lens (#0016) on the separation ring as shown in Fig. 9-7 and screw retaining ring (#0510) down tight. Apply adhesive (#757) to the threads of the lens cell.

3.

Screw the lens cell (#0504) onto the 1x lens cell holder (#0503). Note: Screw the lens cell until you are not able to see its threads any more. Secure the lens cell with the setscrews (#0653x2) to a degree where you can rotate it slightly with your finger. (This is used to focus the lens by rotating the lens cell after fitting the lens cell to the microscope.)

4.

Mount the lens (#L002) to the lens cell as shown in Fig. 9-5, and fit the separation ring (#0505). Mount the lens (#L001) on the separation ring in the direction shown in the figure, and screw the retaining ring (#0506) down tight. Apply adhesive (#757) to the threads of the retaining ring.

5.

Screw the lens cell (#0508) onto the 1.5x lens cell holder (#0507). Note: Screw the lens cell until you are not able to see its threads any more. Secure the lens cell with the setscrews (#0653x2) to a degree where you can rotate it slightly with your finger.

Fig. 9-8

Fig. 9-9

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9. Disassembling/Assembling the Second Objective Lens

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9-2. Assembling the Second Objective Lens 6.

Follow the procedure below to temporarily secure the 1.5x lens cell holder to the magnification variable turret (#0502). (1) Put the lens cell holder on the magnification variable turret and secure it with two alignment clamp screws (J25438). (Fig. 9-10)

Fig. 9-10

(2) Remove either of the alignment clamp screws and temporarily secure the lens cell holder with the hexagonal bolt with washer (#0669). (This is to adjust the optical axis by moving the lens cell after fitting the lens cell to the microscope.) (3) Remove the other alignment clamp screw and temporarily secure the lens cell holder with the hexagonal bolt with washer (#0669). (This causes the 1.5x lens cell holder to be temporarily secured to the center of the hole.) 7.

Position the 1x lens cell holder to the magnification variable turret using alignment clamp screws (J25438) and then secure the holder with the hexagonal bolts with washers (#0669x2). Note: The 1x lens cell holder is not to be temporarily secured but must be permanently secured.

8.

Secure the magnification variable turret to the optical path change-over unit with the setscrews (#0654x2). Adjustments are to be carried out in the following sections. 11-4. Focusing and Optical Axis Adjustment on the Objective Side 11-4-2. Adjustment of the Optical Axis and Parfocality of the 1x Second Objective Lens 11-4-3. Adjustment of the Optical Axis and Parfocality of the 1.5x Second Objective Lens

Make sure that the magnification variable turret smoothly rotates. Now, the second objective lens has been assembled.

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10. Disassembling/Assembling the Optical Path Change-Over Block

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10-1. Disassembling the Optical Path Change-Over Block

- 49 -

10. Disassembling/Assembling the Optical Path Change-Over Block

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10-1. Disassembling the Optical Path Change-Over Block 10-1. Disassembling the Optical Path Change-Over Block * Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1.

Remove the optical unit from the microscope base. (#0664) a) Remove the optical unit. (Refer to 5-2.) b) Attach four legs (J25417) to the removed optical unit. (Fig. 5-11) c) Loosen the setscrews (#0654x2) on the second objective turret unit and remove the unit. (Refer to 9-1 Disassembling the Second Objective Lens.)

J25417

Fig. 10-1

2.

Remove the optical path change-over turret. (Fig. 10-1) (Hexagonal bolts on the center of the turret #0665x2) The removed optical path change-over turret must be handled and stored with care so as not to let it bang against the surroundings.

Fig. 10-3 Fig. 10-2

- 50 -

10. Disassembling/Assembling the Optical Path Change-Over Block

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10-1. Disassembling the Optical Path Change-Over Block When the optical path change-over turret is removed, you will be able to see the special washer (#0558). Underneath the special washer, needle bearings (#0557) are arranged in a circle. (Fig. 10-4) Replace the bearing if it does not move smoothly or if it is damaged or deformed.

Fig. 10-4 *

When the prism in the optical path change-over unit needs to be replaced, perform the following procedure.

8.

Remove the adhesive from the prism that is to be replaced. Remove the prism or prism holder fixing screws from the back of the optical path change-over turret and detach the prism. Scrape off any remaining adhesive and clean the prism using alcohol.

9.

Apply adhesive (#649) to the bonding surface shown in the illustration given in the part list (Fig. 7 for MEA53100) and press each prism against the reference plane for bonding. For prism #0006, press it against the optical path change-over turret holder to bond it to the intended surface. When joining the prism with adhesive, be sure to orientate the prism in the correct direction. Check the prism in the optical path change-over unit, which is pre-assembled and set up for other similar products.

Now, the optical path change-over turret has been disassembled. Wash and clean the components.

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10. Disassembling/Assembling the Optical Path Change-Over Block

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10-2. Assembling the Optical Path Change-Over Block 10-2. Assembling the Optical Path Change-Over Block 1.

Put the special washer, the needle bearing with lubricant (G9124) applied, and another special washer in that order into the location shown in Fig. 10-2.

2.

Attach the optical path change-over turret. (Hexagonal bolts #0665x2) Note: The slit in stop position detecting slit disc (#0562) must be at the center of the photointerrupter on stop position detection sensor board (#0818) when prism #0006 is oriented in the optical path. The slit in zero position detecting slit disc (#0565) must then be at the center of the photointerrupter on zero position detection sensor board (#0818). Refer to 13-3. Adjustment of the Position Sensor in the Optical Path Change-Over Turret.

3.

Checks and adjustments are to be carried out in the following sections. 11-1. Preparation for Optical Adjustment 11-2. Adjustment of Stop Position of Turret 11-3. L100 (Prism in Left Observation Optical System) Optical Axis Adjustment

4.

Attach the second objective turret unit. (Refer to 9-2. Assembling the Second Objective Lens.)

- 52 -

11. Optical Adjustment

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11-1. Preparation for Optical Adjustment Note: Pictures and illustrations in this document are provided for purposes of explaining operation procedures, so they may differ from what you actually see.

11-1. Preparation for Optical Adjustment Tools to be used • • • •

Ti Optical path adjustment mount 2 (J25628) 1-minute reading collimator (J28005) Parallel mirror (J28003) Legs (J25417)

Preparation 1.

2.

Remove the optical unit from the microscope base. (Refer to 5-2 Removing the Optical Unit.)

Fig. 11-1

Attach legs (J25417) to the optical unit. Lift the legs and remove the flat cable support (#0586x3) from the back of the optical unit. (Screw #0663x6) When using a model that has cable clamps mounted on the back of its base, remove the clamps and clean the surface on which they were mounted. (Remove adhesive residue.)

Fig. 11-2

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11. Optical Adjustment

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11-1. Preparation for Optical Adjustment 3. Remove the second objective turret unit from the optical unit. (Refer to 9-1. Disassembling the Second Objective Lens.)

Fig. 11-3

L80/E20 4. Check that R100 (#0007+#0583: prism in the right observation optical system) and L80/E20 (#003+#0585: prism in the left observation optical system) are pressed against the reference plane of the turret.

Reference plane

Reference plane R100

Fig. 11-4

Fig. 11-5

Fig. 11-4

Reference pin

- 54 -

11. Optical Adjustment

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11-1. Preparation for Optical Adjustment 5. Remove the legs from the optical unit and insert the unit into the Ti Optical path adjustment mount 2 (J25628). (Fig. 11-6) Reference plane

Reference plane

Fig. 11-6

6. Place the widest section of the optical unit onto the second post.

Fig. 11-7 Second post

- 55 -

11. Optical Adjustment

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11-1. Preparation for Optical Adjustment 7.

Hold a different position on the unit with one hand. Insert the unit again to pass over the rear reference plane. Pull the optical unit toward the rear reference plane, press it against the side reference plane and then push a lever down to clamp the unit. (Figs. 11-8 and 11-9) 1-minute reading collimator

Fig. 11-8

E100

8.

Rotate the optical path change-over turret to orient Eye100 (#0006) on the turret in the optical path. (Fig. 11-8) Put the 1-minute reading collimator on the optical path of the Ti Optical path adjustment mount 2 (over the open hole above Eye100).

9.

Place the parallel mirror on the top surface of Eye100 so that it can sit well balanced. (Fig. 11-9) Note: When placing the mirror, exercise care so as not to cause scratch on the contact surface of Eye100.

10. Measure the inclination of X and Y of the parallel mirror (Eye100). The reading must be within 6' If the reading is more than 6',please contact to Nikon.

Fig. 11-9

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11. Optical Adjustment

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11-2. Adjustment of Stop Position of Turret 11-2. Adjustment of Stop Position of Turret 1.

Rotate the optical path change-over turret to orient R100 in the optical path. (Refer to Fig. 11-10.)

2.

Put the 1-minute reading collimator on the hole in the top surface of the adjusting base plate. (Refer to Step 4 in 11-1. Preparation: refer to Fig. 11-8.)

Note: Do not move or rotate the position of the 1-minute reading collimator until the adjustment procedures provided in this chapter are completed. 3.

Measure the coordinates of R100 (prism in the right observation optical system). If the reading is more than 6', repeat Step 7 to adjust the mounting position of the click unit (#0547) so that the reading will be within 6'. Adjust the position to bring the reading as close to 0 as possible. Make a note of the value.

4.

Rotate the optical path change-over turret to orient L80/E20 (prism in the left observation optical system) in the optical path. (Fig. 11-11)

Fig. 11-10

Fig. 11-11

5.

Measure the coordinates of L80/E20. The reading must be within 6'. Also, difference in inclination from R100 must be within 3'. Be sure to satisfy these two requirements by adjusting the mounting position of the click unit (#0547).

6.

Orient R100 and measure the coordinates. The reading must be still within 6'.

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11. Optical Adjustment

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11-2. Adjustment of Stop Position of Turret 7.

To adjust the mounting position of the click unit (#0547), do the following: 1) Loosen the click unit fixing screws (#0670x2). 2) Adjust the position of the click unit (#0547) by moving the whole unit while looking into the collimator so that the readings will be within the specifications. (Fig. 11-12) 3) After the click position is adjusted, repeat Steps 1 through 6 to check that the specifications are satisfied.

Fig. 11-12

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11. Optical Adjustment

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11-3. L100 (Prism in Left Observation Optical System) Optical Axis Adjustment

11-3. L100 (Prism in Left Observation Optical System) Optical Axis Adjustment 1.

Rotate the optical path change-over turret to orient L100 (#0007+#0581+#0582) in the optical path. (Figs. 11-13 and 11-14)

2.

Measure the coordinates of L100. The reading must be within 6'. Also, the mutual differences from R100 and L80/E20 must be within 6'. Be sure to satisfy these two requirements by adjusting the angular and rotational direction of L100. To make the adjustments, do the following: 1) Loosen the fixing screws (#0688x3) on #0582 and adjust the angular. 2) Loosen the fixing screws (#0688x4) on #0581 and adjust the rotation direction.

3.

Fig. 11-13 #0582 fixing screw (A total of 3)

After L100 is adjusted, measure the coordinates of R100 and L80/E20. The readings each must be within 6'.

#0581 fixing screw (A total of 4) Each of the following differences in the readings must be within 6': Between R100 and L80/E20, between L80/E20 and L100, and between L100 and R100. If the requirement above is not satisfied, repeat Steps 11-2 and 11-3.

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Fig. 11-14

11. Optical Adjustment

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11-4. Focusing and Optical Axis Adjustment on the Objective Side 11-4. Focusing and Optical Axis Adjustment on the Objective Side Tools to be used • • • • • •

Reference straight tube (for f200 objective) (J25425) Ti Reference reticle (J25573A) Reference revolver for round dovetail (J25433) Crosshair eyepiece (J25220) Dioptric telescope (J29102) Alignment scope (J25172)

Fig. 11-15

11-4-1. Preparation 1.

Remove the diaphragm (#0536) from the optical unit. (Fig. 11-16)

Fig. 11-17

Fig. 11-16 Reference planes

2.

Attach Ti Reference reticle (J25573A) to the reference plane of the optical unit with the diaphragm removed. (Figs. 11-15, 11-16, 11-17 and 11-18) Note: The reference reticle must be correctly oriented.

Fig. 11-18

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11. Optical Adjustment

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11-4. Focusing and Optical Axis Adjustment on the Objective Side 3.

Attach the optical unit to the Ti Optical path adjustment mount 2. Note: This step is not required when you perform the adjustment in this chapter following Steps in 11-1 through 11-3.

4.

Attach the second objective turret to the optical path change-over unit. (Setscrews x2) Check that the click stop appropriately works.

Fig. 11-19

5. 6.

Orient Eye100 in the optical path. Attach the reference revolver for round dovetail (J25433) to the objective lens with the two fixing screws and attach the reference straight tube (for objective J25575) on it. (Fig. 11-18) Note: Since the focal position changes during focusing, remove the dustproof glass (#AL01) from the microscope and insert the glass into the Ti Optical path adjustment mount 2. (Figs. 11-20 and 11-21)

Fig. 11-20 Fig. 11-21

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11. Optical Adjustment

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11-4. Focusing and Optical Axis Adjustment on the Objective Side 11-4-2. Adjustment of the Optical Axis and Parfocality of the 1x Second Objective Lens 1.

Fit the crosshair eyepiece (J25220) to the reference straight tube and focus on the crossline. (Fig. 11-18)

2.

Set the magnification change-over lever to 1x.

3.

Temporarily focus on the reference reticle. Steps: Loosen the setscrews #0653. While looking through the eyepiece, rotate the 1x lens cell. (Fig. 11-22)

Fig. 11-22 4.

Make the crossline in the eyepiece coincide with the center of the reference reticle by adjusting the angular and rotational direction of the mirror unit (#0009+#0534+#0535). (Fig. 11-23) To make the adjustments, do the following: 1) Loosen the fixing screws (#0668x4) on #0535 and adjust the angular. 2) Loosen the fixing screws (#0688x4) on #0534 and adjust the rotation direction. • Specification: Within 0.004 in.

5.

Fit the dioptric telescope (J29102) to the eyepiece lens and bring each crossline into focus. (Fig. 11-24)

6.

Rotate the lens cell to bring the three lines in the dioptric telescope, crosshair eyepiece and reference reticle into focus.

7.

When they are in focus, tighten the screws of the lens cell. • Specification: Within 0.024 in.

8.

Remove the dioptric telescope and check that the crossline in the eyepiece coincides with the center of the reference reticle. If they do not, repeat Step 4 to adjust the angular and rotational direction of the mirror unit until they coincide.

9.

When the optical path is changed from "E100" to "R100" and "L80/E20", check that the amount of misalignment at the center of the reticle on the respective positions is within 0.006 in. (within 1.5 graduations on the graduated scale on the eyepiece).

Fig. 11-23

- 62 -

#0534 fixing screw (A total of 4)

#0535 fixing screw (A total of 4)

Fig. 11-24

11. Optical Adjustment

R-4820.A

11-4. Focusing and Optical Axis Adjustment on the Objective Side 11-4-3. Adjustment of the Optical Axis and Parfocality of the 1.5x Second Objective Lens 1.

Change the magnification change-over lever to 1.5x.

2.

Temporarily focus on the reference reticle. Steps: Loosen the setscrews (#0653x2). While looking through the eyepiece, rotate the 1.5x lens cell. (Fig. 11-25) Note: If the reference scale line is not visible in the view of eyepiece, loosen the bolts (#0669x2: Fig. 9-3) securing the 1.5x lens cell holder (#0507) and move the 1.5x lens cell until the line becomes visible.

3.

Loosen the securing bolts on the lens cell holder. (#0669x2) If you loosened them in Step 2, leave them as they are and proceed to the next step.

4.

Adjust the position of the lens cell to make the crossline in the eyepiece coincide with the center of the reference reticle. (Fig. 11-25) When they coincide, secure the lens cell with the hexagonal bolts (#0669x2). • Specification: Within 0.004 in.

5.

Fit the dioptric telescope to the eyepiece lens and bring each crossline into focus.

6.

Rotate the lens cell to bring the three lines in the crosshair eyepiece, dioptric telescope and reference reticle into focus.

7.

When they are in focus, tighten the setscrews. • Specification: Within 0.024 in.

8.

Remove the dioptric telescope and check that the crossline in the eyepiece coincides with the center of the reference scale. If they do not, rotate the lens cell until they coincide.

9.

When you change the magnification change-over lever from 1.0x to 1.5x or 1.5x to 1.0x, check that the amount of misalignment from the optical axis and focus satisfies the following specifications: • Optical axis: Within 0.06 in. • Focus: Within 0.028 in.

Now, the objective side has been adjusted. 10. Attach the optical unit to the microscope base. (Refer to 5-3. Installing the Optical Unit.)

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Fig. 11-25

11. Optical Adjustment

R-4820.A

11-5. Preparations Common to Overall Optical Adjustments 11-5-1. Setting Carry out the preparatory steps described in this section before starting the overall adjustment. The main purpose of this section is the same as that of the previous section (11-4. Focusing and Optical Axis Adjustment on the Objective Side). If you have carried out the previous section, you can skip this section. 1. 2.

Lay the microscope base with its rear side down. Attach the optical unit while pressing it against the reference positions (on the left and back sides) of the microscope base. (Hexagonal bolts #0203x6) (Refer to 5-3. Installing the Optical Unit.)

3.

Remove four out of the six vibration dampening rubber legs shown in the figure and replace them with four tool legs (J25417). After replacement, bring the microscope back to horizontal. (Fig. 11-26)

Tools to be used • • • • • •

Crosshair eyepiece (J25220) Dioptric telescope (J29102) Reference revolver for round dovetail (J25433) Reference straight tube (for objective) (J25425) Ti Reference reticle (J25573A) Ti Reference straight tube for body tube (J2575)

Fig. 11-26

Preparation (for focusing the reference reticle and adjustment of optical axis) 1.

Attach the optical unit to the inside of the microscope while pressing it against the reference plane of the microscope base. (Refer to 5-3. Installing the Optical Unit.)

2.

Remove the diaphragm (#0536) from the optical unit.

3.

Attach the Ti Reference reticle (J25573A) to the optical unit while pressing it against the reference plane. Note: The Ti Reference reticle must be correctly oriented.

4.

Bring the microscope back to horizontal.

5.

Remove the protective cover and dummy sledge. (Refer to 6-1. Disassembling the Up-Down Movement Unit.)

6.

Remove the fixing screw (#0122x4) so that you can easily remove the dustproof glass holder when making adjustments. Note: Take care not to lose the rubber sheet under the dustproof glass holder.

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11. Optical Adjustment

R-4820.A

11-5. Preparations Common to Overall Optical Adjustments 7.

Loosen the fixing screws (#0216x2) to detach the straight tube from the left-side port mount (if attached). (This is to allow you to insert your finger into the hole when focusing the second objective lens and make adjustment of its optical axis.)

Fig. 11-27

8. 9.

Attach the reference revolver for round dovetail (J25433) to the microscope. (Hexagonal bolts x2) Turn the optical path change-over dial to E100.

11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment 1.

1x objective lens 1) Turn the magnification change-over handle to "1x". 2) Fit the crosshair eyepiece (J25220) to the reference straight tube for objective (J25425) with the dustproof glass holder attached. Turn the diopter ring to bring the crossline into focus. 3) Adjust the focus of the reference reticle by rotating the second objective lens cell (moving the lens position up or down) on the 1x side. To make the adjustment, do one of the following: a) Insert your finger into the hole in the left-side port and rotate the second objective lens cell on the 1x side. b) Remove the dustproof glass holder and rotate the second objective lens cell on the 1x side. 4) Follow the procedures below to make the crosshair eyepiece coincide with the center of the reference reticle. a) Loosen the hexagonal bolts with washers (#0668x4) that secure the adjustment prism to the main plate to a degree that the prism can be moved by hand (horizontal adjustment). b) Loosen the hexagonal bolts with washers (#0668x4) that secure the adjustment prism to its support plate to a degree that the prism can be moved by hand (vertical adjustment). c) Manually adjust the position of the prism vertically and horizontally. 5) The amount of misalignment between the crosshair eyepiece and the center of the reference reticle must be within 0.004 in. (within 1 graduation on the graduated scale on the eyepiece).

After confirmation, tighten the hexagonal bolts with washers (#0668x8) that mount the adjustment prism.

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11. Optical Adjustment

R-4820.A

11-5. Preparations Common to Overall Optical Adjustments 6) When the optical path change-over dial is changed from "E100" to "R100" and "L80/E20", check that the degree of misalignment at the center of the reticle on the respective positions satisfies the relevant specifications. Specification: Within 0.006 in. (within 1.5 graduations on the graduated scale on the eyepiece) 2.

1.5x objective lens (The following is compliant with x1.) 7) Turn the magnification change-over handle to "1.5x". 8) Using the same procedure in Step 3), adjust the focus of the reference reticle by rotating the second objective lens cell (moving the lens position up or down) on the 1.5x side. 9) Adjust the focus by moving the position of the 1.5x second objective lens cell holder that is temporarily secured to the magnification variable turret to make the crosshair eyepiece and the center of the reference reticle coincide.

Adjustment procedure: Insert your finger into the hole in the left-side port and move the second objective cell on the 1.5x side. Alternatively, remove the dustproof glass holder and adjust the focus by moving the position of the second objective lens cell on the 1.5x side. After adjustment, tighten the hexagonal bolts with washers (#0669x2) that mount the second objective lens cell. Now, the temporary adjustment of the focus of the reference reticle and the optical axis adjustment have been completed. Then, carry out focusing.

11-5-3. Focusing 1.

Fit the dioptric telescope to the crosshair eyepiece. Be sure to attach the dustproof glass holder. (Notes) Set the indicator of the dioptric telescope to "0". Turn the diopter ring to bring the double crosshair reticle into focus. Turn the correction ring to bring the scale of the crosshair eyepiece into focus.

2.

Turn the optical path change-over dial to "E100".

3.

Turn the magnification change-over handle to "1x" and "1.5x" and check that the focus of the reference reticle that is observed with the graduated scale on the eyepiece diopter satisfies the following specification. If the specification is not satisfied, as with the temporary adjustment, make fine adjustment by rotating the second objective lens cell (moving the lens position up or down) on the 1x and 1.5x sides. Specification: Within ± 0.024 in. (within six graduations on the graduated scale on the eyepiece) When the magnification change-over handle is changed to a range between 1x and 1.5x: Within 0.028 in. (within seven graduations on the graduated scale on the eyepiece)

4.

Upon completion of adjustment, remove the dustproof glass holder from the microscope. Insert the machined hex key through the opening and secure the second objective lens cells on the 1x and 1.5x sides with the setscrews (#0669x2 for each).

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11. Optical Adjustment

R-4820.A

11-5. Preparations Common to Overall Optical Adjustments 5.

Tighten the setscrews and then reattach the dustproof glass holder to the microscope. (Put the holder back in place.) Then, reconfirm the focus of the reference reticle with the magnification change-over handle turned to "1x" and "1.5x" positions.

Now, the focusing has been completed. Then, reconfirm the optical axis. Checking Optical Axis 6.

Check that the optical axis satisfies the following specification: Specification: Within ±0.004 in. (within one graduation on the graduated scale on the eyepiece) If the specification is not satisfied, repeat Steps in "11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment" to make the adjustment again.

7.

Turn the magnification change-over handle to "1.5x" and check that the optical axis satisfies the following specification: Specification: Within ±0.004 in. (within one graduation on the graduated scale on the eyepiece) If the specification is not satisfied, repeat Steps in "11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment" to make the adjustment again.

8.

When the optical path change-over dial is changed from "E100" to "R100" and "L80/E20", check that the degree of misalignment at the center of the reticle on the respective positions satisfies the relevant specifications. Specification: Within 0.006 in. (within 1.5 graduations on the graduated scale on the eyepiece)

9.

Works to be Performed Upon Completion of Adjustment 1) Secure the dustproof glass holder. (Fixing screws x4) 2) Note: Be sure to insert the rubber sheet between the dustproof glass holder and the microscope base. 3) After reconfirmation, apply adhesive (#0757) to the threads of setscrews (#0669x2) that are secured to the second objective lens cells on the 1x and 1.5x sides.

Now, the focusing and optical axis adjustment on the objective side using the reference reticle has been completed. Then, move on to the focusing and optical axis adjustment on the observation system.

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12. Overall Optical Adjustment

R-4820.A

12-1. Focusing and Optical Axis Adjustment on the Observation System 12-1. Focusing and Optical Axis Adjustment on the Observation System Note: In this chapter, you use electrically-driven functions for some steps. Before starting this chapter, check that an electrical system is OK, or complete "13. Electrical Adjustments".

Tools to be used • • • • •

Crosshair eyepiece (J25220) Dioptric telescope (J29102) Alignment scope (J25172) Light post (product) Ti Reference reticle (J25573A)

• Revolver (product) • 10x objective lens (product) • Ti Reference straight tube for f210 body tube (J25575) • Ti Tool body tube base (J25574)

12-1-1. Preparation 1.

Attach the Ti Reference reticle (J25573A) to the optical unit (Refer to 11-4. Focusing and Optical Axis Adjustment on the Objective Side).

2.

Remove the four rubber legs and replace them with four tool legs (J25417). (Refer to 11-5-1 Setting in the Preparations Common to Overall Optical Adjustments section.)

3.

Bring the microscope back to horizontal.

4.

Install the light post (transmitted lighting). (Refer to 5-1 Removing the Accessories.)

5.

Attach the Ti Tool body tube base (J25574) to the microscope.

6.

Loosen the screw (#0217) securing the lens cell (#0112).

7.

Loosen the hexagonal bolts in the lens cell holder (#0111) to a degree it can be moved by hand. (Hexagonal bolts #0207x3)

8.

Attach the Ti Reference straight tube for f210 body tube (J25575) to the Ti Tool body tube base.

9.

Fit the crosshair eyepiece lens (J25220) to the Ti Reference straight tube (for body tube) and turn the diopter ring to bring the crossline into focus.

10. Turn the power on, turn the optical path change-over dial to "E100" and then turn the power off.

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Fig. 12-1

12. Overall Optical Adjustment

R-4820.A

12-1. Focusing and Optical Axis Adjustment on the Observation System 12-1-2. Focusing and Optical Axis Adjustment 1. Rotate the lens cell to bring the reference reticle into focus. Insert your finger into a space in the tool to rotate the lens cell and adjust the focus by moving the lens position up or down. (This should be carried out as a temporary adjustment.) 2. Make the crossline in the eyepiece coincide with the center of the reference reticle. Make this adjustment by rotating the lens cell by hand. After adjustment, secure the lens cell holder. (Hexagonal bolts #0207x3) 3. Fit the dioptric telescope to the crosshair eyepiece. 4. Bring the scale of each of the reference reticle, crosshair eyepiece and dioptric telescope into focus. The adjustment method is the same as in Step 1. • Specification: Within 0.012 in.

Checking Optical Axis Remove the dioptric telescope and check that the crossline in the eyepiece coincides with the center of the Fig. 12-3 reference scale. If they do not, repeat Steps from 2. Specification: Within 0.008 in. Eccentricity with respect to the reference reticle must be within 0.004 in. Now, the focusing and optical axis adjustment on the observation system has been completed. Then, move on to the beam adjustment.

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12. Overall Optical Adjustment

R-4820.A

12-1. Focusing and Optical Axis Adjustment on the Observation System 12-1-3. Beam Adjustment 1. 2. 3.

Attach the revolver and mount the 10x objective lens. Fit alignment scope (J25172) to the reference straight tube (tube). Measure the beam and check that it satisfies the following specification.

Specification: Within 5' If it fails to satisfy the specification, perform Step 4 described below to make the adjustment. 4.

Adjust the beam using the three alignment screws in lens cell holder (#0538). Note 1. These three alignment screws are not spaced 120 degrees apart with respect to the center of the lens, so when you turn one of them, the lens may not move in your desired direction. Therefore, you should not adjust the beam using one or two screws. Be sure to use the three screws to appropriately adjust the beam. (Fig. 12-4) 2. The beam adjustment may result in a slight misalignment of the optical axis. Be sure to reconfirm the optical axis whenever you have made beam adjustment. For details on rechecking and readjusting for the optical axis, refer to 12-1-2. Focusing and Optical Axis Adjustment.

Fig. 12-4

Now, the focusing and optical axis adjustment on the observation system has been completed. Then, move on to the focusing and optical axis adjustment on the side port.

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12. Overall Optical Adjustment

R-4820.A

12-2. Focusing and Optical Axis Adjustment on the Side Port 12-2. Focusing and Optical Axis Adjustment on the Side Port Tools to be used • • • • • • •

Light post (product) Stage (product) Crosshair eyepiece (J25220) Dioptric telescope (J29102) Revolver (product) Grip sample (J28004) Dark crosshair sample (J25194)

• 10x objective lens (product) • 1x objective lens (product) • Ti Reference straight tube for body tube (J25575) • Ti Tool body tube base (J25574) • Ti Output port straight tube A or B J25572 A/B • Tool for adjusting infinite optical axis difference (J28171)

12-2-1. Preparation (for focusing and optical axis adjustment on the right- and left-side ports) 1.

Install the light post (transmitted lighting).

2.

Attach the revolver and mount the 10x objective lens.

3.

Attach the stage.

4.

Fit the dark crosshair sample to the stage. Its deposited surface must face the objective side.

5.

Attach the Ti Tool body tube base (J25574).

6.

Attach the Ti Reference straight tube for f210 body tube (J25575) to the Ti Tool body tube base.

7.

Fit the crosshair eyepiece to the Ti Reference straight tube for body tube (J25575). (Fig. 12-5)

8.

Turn the diopter ring to bring the crossline into focus.

Fig. 12-5

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12. Overall Optical Adjustment

R-4820.A

12-2. Focusing and Optical Axis Adjustment on the Side Port 12-2-2. Optical Axis Adjustment Note: When you use tool for adjusting infinite optical axis difference (J28171) instead of the 10x objective lens, Steps 2 and 3 are not necessary. 1.

Turn the power on and turn the optical path change-over dial to "E100".

2.

Using the focusing handle, bring the sample into focus.

3.

By moving the stage, make the crosslines of eyepiece lens coincide with the center of the sample.

4.

Attach the Ti Output port straight tube A (J25572A) to the left-side mount. (Fixing screws x2) If the straight tube is attached to the left-side mount, attach the Ti Output port straight tube B (J255572B) and secure it. (Fixing screws x2)

Fig. 12-6

5.

Fit the crosshair eyepiece to the Ti Output port straight tube A or B. (Fig. 12-6)

6.

Turn the optical path change-over dial to "L100".

7.

Loosen the fixing screws (#0209x4) securing the side mount and adjust its position to make the center of the sample coincide with the center of the crosshair eyepiece. After adjustment, tighten the screws.

Now, adjust the optical axis of the right-side port. 8. 9. 10. 11.

Replace the Ti Output port straight tube A or B with the right-side mount. (Fixing screws x2) Turn the optical path change-over dial to "R100". Following Steps 5 through 7, adjust the optical axis of the right-side port. After adjustment, turn the optical path change-over dial to "E100".

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12. Overall Optical Adjustment

R-4820.A

12-2. Focusing and Optical Axis Adjustment on the Side Port 12-2-3. Focusing Adjustment 1. 2. 3. 4.

Replace the 10x objective lens with the 1x lens. Fit the dark crosshair sample to the stage. Its deposited surface must face the objective side. Fit the crosshair eyepiece and dioptric telescope to the observation system. Using the focusing handle, bring the dark crosshair sample into focus. (In this step, bring it into focus roughly.)

5. 6.

Set the diopter ring on the crosshair eyepiece and the indicator of the dioptric telescope to "0". Turn the diopter ring on the dioptric telescope to bring the double crosshair reticle into focus. From now on, keep your hand off the diopter rings on the crosshair eyepiece and on the dioptric telescope.

7. 8.

Using the focusing handle, bring the dark crosshair sample into focus. Press the "Z-RESET" switch on the Ti-E front panel to set the Z axis display to "0".

7.

Remove the crosshair eyepiece with the dioptric telescope attached to it from the reference straight tube (for body tube) of the observation system and attach it to Ti Output port straight tube A or B on the right-side port.

8.

Using the focusing handle, bring the dark crosshair sample into focus.

9.

Read the Z axis display. (Measure an amount of misalignment with respect to the focal position of the observation system.)

Fig. 12-7

• Specification: Within ±0.008 in. If the specification is satisfied, proceed to Step 12. If not, proceed to Step 10. 10. Detach the side mount (#0139). (#0209x4) 11. Do one of the following to adjust the focal position: (Fig. 12-7) • Remove the existing adjustment washer. • Insert an appropriate adjustment washer between the side mount and microscope base (#0101). Tips • If the amount of misalignment is a negative value, insert an adjustment washer between the side mount and the microscope base. • If the amount of misalignment is a positive value, remove the adjustment washer between the side mount and the microscope base. The following four types of adjustment washers are provided for focusing: 0.039 in. (#0151A), 0.2 in. (#0151B), 0.012 in. (#0151C), 0.039 in. (#0151D) Note: Be sure to insert a pair of adjustment washers of the same thickness.

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12. Overall Optical Adjustment

R-4820.A

12-2. Focusing and Optical Axis Adjustment on the Side Port Now, adjust the focus of the left-side port. 12. Remove the crosshair eyepiece with the dioptric telescope attached to it from the Ti Output port straight tube A or B of the right-side port and attach it to the reference straight tube (for body tube) of the observation system. 13. Following the same procedures in Steps 4 through 8, adjust the focus of the observation system and set the Z axis display to "0". 14. Turn the optical path change-over dial to "L100". 15. Remove the crosshair eyepiece with the dioptric telescope attached to it from the reference straight tube (for body tube) of the observation system and attach it to Ti Output port straight tube A or B on the left-side port. 16. Following the same procedures in Steps 7 through 11, adjust the focus of the left-side port. 17. Following the procedures given in 12-2-2, check optical axis on the right- and left-side ports and, if necessary, readjust it. 18. When it is found that the specification is not satisfied when rechecking, readjust the focus following the procedures in Steps 3 through 16. Now, the focusing and optical axis adjustment on the right- and left-side ports has been completed.

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13. Electrical Adjustments

R-4820.A

13-1. Encoder Adjustment Tools • Oscilloscope

13-1-1. Preparation 1. Remove the up-down movement unit. (Refer to 5-2. Removing the Optical Unit, and 6-1. Disassembling the Up-Down Movement Unit.) 2. Connect J2 connector on preamplifier board (#0702) and J4 connector on interface board (#0802) using relay cable (#0821). 3. Connect the oscilloscope probes to the preamplifier board as described below. (Refer to Fig. 13-1.) • CH1 ⇒ TP1 (AX) • CH2 ⇒ TP2 (BX) • GND ⇒ TP3 (A GND) 4. Set the oscilloscope as shown below. • Sweep time ⇒ X-Y (Lissajous waveform mode) • Input mode ⇒ AC • Voltage ⇒ 1 V/div 5. Set the variable resistors on the preamplifier board beforehand as described below. • Turn VR1 and VR3 to the center position. • Turn VR2 and VR4 in the clockwise direction as far as they will go (to minimize the gain of the amplifier). 6. Connect the AC adapter output cable to the microscope and plug in the microscope to turn it on.

TP 2

VR 3

VR 4

TP 1

VR 1

GND

VR 2

Fig. 13-1 - 75 -

13. Electrical Adjustments

R-4820.A

13-1. Encoder Adjustment 13-1-2. Adjustment of Tilt of Encoder Head • Tilt the head toward the glass scale rotating about the LED sleeve so that the waveform is maximized. Note 1: The waveform at this point does not need to be a perfect circle. 2: The index, LED, amplitude and center level must be adjusted while moving the up-down movement unit. 1) Slightly loosen the two hexagonal bolts securing the index. Rotate the index in the direction of rotation about the LED sleeve to a position where the Lissajous waveform is maximized and tighten the bolts to secure the index.

LED sleeve

Bolt securing index block

Index

Fig. 13-2 2) In doing this, the size of the Lissajous waveform alternately changes between small and large. Secure the index with a large waveform d being displayed. Note: Note that securing the index with a small waveform makes the counting direction reverse.





Fig. 13-3

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13. Electrical Adjustments

R-4820.A

13-1. Encoder Adjustment • If the shape of Lissajous waveform is oval: 1.

Loosen the setscrew securing the LED to the encoder head and rotate the LED until the shape of the waveform becomes perfectly circular.

Setscrews securing LED

Note: Regardless of size, make the shape of the waveform as close to a perfect circle as possible. 2.

Tighten the setscrew at a point where the shape of the Lissajous waveform becomes a circle.

Fig. 13-4

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13. Electrical Adjustments

R-4820.A

13-1. Encoder Adjustment 13-1-3. Amplitude (Gain) Adjustment of the Preamplifier Board 1. Change the oscilloscope settings as shown below. • Input mode: AC • Sweep time: 2 msec • Voltage: 1 V/div 2. Adjust the following variable resistors so that the PP value of the waveform is 3.2 V while moving the up-down movement unit. Refer to Fig. 13-1 for the locations of the variable resistors on the board. • Use VR2 for AX adjustment. • Use VR4 for BX adjustment.

Specification: PP value: 2.4 to 4 V (Reference value: 3.2 V ± 0.2 V for a velocity of 0.394 in./sec) If the signal is saturated as shown in Fig. 13-6, carry out the following adjustment of the variable resistors. (The waveform shown in Fig. 13-5 must be obtained.) Refer to Fig. 13-1 for the locations of the variable resistors on the board. • VR1 for AX adjustment • VR3 for BX adjustment

2.4 ~4(V)

Fig.① 13-5

Fig.② 13-6

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13. Electrical Adjustments

R-4820.A

13-1. Encoder Adjustment 13-1-4. Center Level (Offset) Adjustment of the Preamplifier Board 1. Change the oscilloscope settings as shown below. • Sweep time ⇒ 2 msec • Voltage ⇒ 2 V/div • DC/AC ⇒ DC 1. Use the vertical position knob on the oscilloscope to set the GND position to a point three divisions below the center of the monitor. The GND position setting must be identical to both CH1 and CH2. With this adjustment, the centerline of the oscilloscope display corresponds to 6-volt position. 2. View CH1 on the monitor. (Fig. 13-7) 3. Adjust "VR1" so that amplitude of the waveform is symmetrical above and below the horizontal centerline. (Fig. 13-8) Refer to Fig. 13-1 for the locations of the variable resistors on the board.

Fig.① 13-7

Fig.②13-8

4. View CH2 on the monitor. 5. Make the same adjustment in 3 above using "VR3".

Specification: ±0.2 V with respect to the center level

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13. Electrical Adjustments

R-4820.A

13-1. Encoder Adjustment 13-1-5. Confirmation of Lissajous Waveform Set the oscilloscope as shown below. • Input mode ⇒ DC • Sweep time ⇒ X-Y mode • Voltage ⇒ 2 V/div Observe the entire length of the Lissajous waveform and check that changes in its shape are within the specification. Specification: Phase difference: 90°±15°

Limit samples

9 0 ゜-15゜

90 ゜ ±0 ゜

Fig. 13-9

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9 0 ゜+15゜

13. Electrical Adjustments

R-4820.A

13-1. Encoder Adjustment 13-1-6. Signal Specifications Center levels (EApp, EBpp): Amplitude (VApp, VBpp): At a velocity of 10 mm/sec: Amplitude difference (VApp - VBpp): Phase difference:

(Vcc/2) V ±0.2 V, Vcc=12 V +2.4 V to 4.0 V +3.2 V ±0.2 V The voltage of phase (A or B) having small amplitude must be ±10% or below. 90°±15°

A相 Phase A

EApp

EBpp

VBpp

VApp

Phase B相 B

0V Fig. 13-10

Check that the above specifications are satisfied. Reattach the up-down movement unit to the microscope by reversing the procedures described in the preparation.

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13. Electrical Adjustments

R-4820.A

13-2. Upper- and Lower-Limit Sensor Adjustment 13-2-1. Checking Limit Sensor Operation 1. Set the microscope to horizontal. 2. Connect the tester probe to the following connectors. • Upper limit sensor ⇒ J1 connector, pin 3 • Lower limit sensor ⇒ J1 connector, pin 5 • GND ⇒ J1 connector, pin 2 3. After power-up, check that both the upper and lower limit sensors appropriately function. If they fail, replace them.

13-2-2. Limit Sensor Mounting Position Adjustment 1. Operate the up-down movement handle until the distance between the up-down movement unit mounting surface and the revolver mounting surface is 2.618 in.

Revolver mounting surface

Up-down movement unit mounting surface

Fig. 13-11 2. Adjust the position where the limit sensor board (#0705) or chopper is installed so that the lower limit sensor detects chopper (#0412) at this position. For the limit sensor board, adjust its mounting position by moving it up or down. For the chopper, adjust its mounting position by moving it in the direction of rotation. After adjustment, tighten the hexagonal bolts.

Upper limit sensor

Chopper

Lower limit sensor

Fig. 13-12

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13. Electrical Adjustments

R-4820.A

13-3. Adjustment of the Position Sensor in the Optical Path Change-Over Turret

13-3-1. Adjustment of the Stop Position Sensor 1. Have the bearing dropped into the groove (for click-stop) in the optical path change-over turret.

Click stop

Bearing

Fig. 13-13 2. Adjust the position in the rotational direction of the stop position detecting slit disc (#0562) so that the distance between the notch on the left side of the slit disc and the photointerrupter on stop position detection sensor board (#0818) is identical to that between the notch on the right side of the slit disc and the photointerrupter on stop position detection sensor board (#0818). (Figs. 13-14 and 13-15)

#0562 Stop position detecting slit disc

Photointerrupter

Notch

Fig. 13-14

#0562 Stop position detecting slit disc

Fig. 13-15

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13. Electrical Adjustments

R-4820.A

13-3. Adjustment of the Position Sensor in the Optical Path Change-Over Turret

13-3-2. Adjustment of the Zero Position Detection Sensor 1. Rotate the optical path change-over turret to have the bearing dropped into the groove (for click-stop). Orient "E100" in the optical path. 2. Adjust the position in the rotational direction of the zero position detecting slit disc (#0565) so that the distance between the notch on the left side of the disc and the photointerrupter on zero position detection sensor board (#0818) is identical to that between the notch on the right side of the disc and the photointerrupter on zero position detection sensor board (#0818). (Figs. 13-16 and 13-17) Photointerrupter

#0565 Zero position detecting slit disc

Notch

Fig. 13-16

#0565 Zero position detecting slit disc

Fig. 13-17

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Appendix: Test Mode

R-4820.A

Test Mode This section explains the test mode. 1.

Test Mode Hierarchy Turn on power

Product program

Special operation 1

Special operation 3

Special operation 2

To next page

Page 1 (Checking switch inputs)

Press [b].

Press [b]. Page 1 (Motorized accessories)

Press [c]. Page 2 (Reading limit sensors)

Press [b].

Page 1 (Speed control parameters)

Press [c].

Press [c]. Page 2 (Control speed gain)

Page 2 (Micrometer)

Page 3 Page 3 (Checking set signals)

Page 4

(Automatic adjustment division boundary line)

Page 3 (Stage)

Page 4 (Offset zero-position setting)

(Checking the piezoelectric focus drive)

Page 4 (Shutter)

Page 5 (PFS nosepiece information)

Page 5 (DIA illumination)

Page 6 (Z motor drive and opticalpath change motor drive)

Page 7 (Bottom and offset lens motor drives)

Main Body System Information

Displaying/Editing PFS Parameters [Operation and page change]

Page 6 (Intensilight)



Page 7 (DSC) Page 8 (Handle and knob data)

On any page, use the four optical path change switches. [a]: Changes the value. (+) [b]: Changes the page. (-) [c]: Changes the page. (+) [d]: Changes the value. (-)

Page 9 (PFS nosepiece PID) Page 10 (Hub-side nosepiece PID) Page 11

Hub System Information

(Ergonomic controller and joystick information)

a b c d

- 85 -

Appendix: Test Mode

R-4820.A

Test Mode From previous page

Page 1

Displaying/Editing Software DIP Switches

Special operation 4

Special operation 5

Page 1

Displaying/Editing PFS Parameters

[Operation and page change] •

On any page, use the four optical path change switches. [a]: Changes the value. (+) [b]: Changes the item. (-) [c]: Changes the item. (+) [d]: Changes the value. (-)

a b c d

- 86 -

Appendix: Test Mode

R-4820.A

Test Mode 2.

Displaying Information About the Main Body System On the front VFD, the operator can display connection and other information for the purpose of main-body maintenance. The operator can also command to revolve (in both CW and CCW directions) and stop motors used for electric devices.

[Functions] • • • • • • • • •

Checking/displaying switch input Flashing LEDs sequentially Checking/displaying limit sensor reading Checking/displaying motorized accessory reset signal connection Checking/displaying PFS nosepiece address Checking/displaying PFS nosepiece stop sensor Checking/displaying piezoelectric focus-drive input signal Operating piezoelectric focus-drive output signal Commanding to revolve (CW/CCW) and stop focus drive motor • Commanding to revolve (CW/CCW) and stop optical path a change motor b • Commanding to revolve (CW/CCW) and stop bottom drive motor • Commanding to revolve (CW/CCW) and stop offset lens motor

c d

[Start/shutdown (special operation 1)] • Use the four optical path change switches located on the front of the main body. [a][a][d][d][b][c][b][c][c][c]

Press the above key sequence within 10 seconds to display the "main-body system information page 1."

[Shutdown] • Press the [BRIGHTNESS] button four times within 5 seconds.

[Operation and page change] • On any page, use the four optical path change switches. [a]: Changes the value. (+) [b]: Changes the value. (-) [c]: Changes the page. (+) [d]: Changes the page. (-)

[Restrictions] • Each switch does not have an inherent function, but its behavior varies with each page. Confirm the different behaviors as stated in the specification. - 87 -

Appendix: Test Mode

R-4820.A

Test Mode 2-1

Page 1 -- Checking switch inputs This page allows the operator to switch inputs on the VFD. At the same time, LEDs flash sequentially. Check for any LEDs that are not lit.

0

a P b L

1

1 :

2

*

3

F *

4

: *

5

* *

6

* *

7

* *

8

* *

9

* *

10

11

12

13

14

15

16

17

18

19

* R

* :

* *

* *

* *

* *

*

*

*

*

a0-1 a5-15 b5-8 b12-18

: : : :

Page number Indication of input of front switches (=0: no input; Indication of input of switches on left side surface (=0: no input; Indication of input of switches on right side surface (=0: no input;

=1: with input) =1: with input) =1: with input)

Pressing a switch changes the display at the corresponding position on the VFD.

- 88 -

Appendix: Test Mode

R-4820.A

Test Mode 2-2

Page 2 -- Reading limit sensors This page allows the operator to read limit sensors. 0

a P b

1

2

2

3

[ Z

4

S :

5

e *

6

n *

7

s

8

o P

9

r :

10

11

12

13

14

15

16

h

e B

c :

k *

]

*

c *

17

18

19

O

F

F

17

18

19

a0-1 b5 b6 b10 b11 b15 b17-19

: : : : : : :

Page number Z upper-limit sensor Z lower-limit sensor Optical path change origin sensor Optical path stop sensor Bottom prism in/out sensor Sensor on/off state

(=[1]:shaded; =[0]:exposed) (=[1]:shaded; =[0]:exposed) (=[1]:shaded; =[0]:exposed) (=[1]:shaded; =[0]:exposed) (=[1]:shaded; =[0]:exposed)

Change optical path, [a] [d] : Toggles the sensor on and off.

2-3 Page 3 -- Checking set signals This page displays the set signals for motorized accessories. 0

a P b

1

3

2

3

[ -

4

S -

5

e -

6

t -

7

8

s -

9

i -

10

11

12

13

14

15

g -

n

a -

l

] -

-

16

a0-1 b3-7 b9-10 b12 b14-15

: : : : :

Page number Hub connection PFS nosepiece connection Offset lens drive knob connection Piezoelectric focus drive connection

([-----]: none; [HUB-A]: HUB-A; ([--] : none; [PR]: connected) ([-]: none; [K]: connected) ([--]: none; [PZ]: connected)

No function

- 89 -

[HUB-B]: HUB-B)

Appendix: Test Mode

R-4820.A

Test Mode 2-4 Page 4 -- Checking the piezoelectric focus drive This page displays the output voltage DA setting for piezoelectric focus drive and the input voltage AD value for position. 0

a P b

1

2

4

3

[ I

4

P N

5

e :

6

z -

7

o -

8

9

10

Z -

-

11

12

13

14

15

16

17

18

c O

h U

e T

c :

k -

] -

-

-

19

a0-1 : Page number b6-9 : Piezoelectric input voltage AD value b15-18 : Piezoelectric output voltage DA value (default: 0)

Change optical path, [a] [d] :

Changes piezoelectric output voltage DA value.

2-5 Page 5 -- PFS nosepiece information This page displays information about the PFS nosepiece. 0

a P b

1

2

5 R

3

[ :

4

P -

5

F -

6

7

8

O

R L

S

9

e :

10

11

v -

o -

12

13

14

15

16

17

18

19

c M

h :

e -

c

k K

] :

-

a0-1 b4 b5 b10 b11 b15 b19

: : : : : : :

Page number Stop sensor Address sensor Offset lens lower-limit sensor Offset lens upper-limit sensor Dichroic mirror in/out sensor Offset lens drive knob switch input

(=[1]:IN; =[0]:OUT; =[-]:not connected) (=[1]~[6]:address; =[0]:undefined; =[-]:not connected) (=[1]:IN; =[0]:OUT; =[-]:not connected) (=[1]:IN; =[0]:OUT; =[-]:not connected) (=[1]:OUT; =[0]:IN; =[-]:not connected) (=[1]:ON; =[0]:OFF; =[-]:not connected)

No function

- 90 -

Appendix: Test Mode

R-4820.A

Test Mode 2-6

Page 6 -- Z motor drive and optical-path change motor drive This page allows the operator to control the Z motor drive and optical-path change motor drive. 0

a P b

1

2

3

4

5

6 P

a

6

7

Z h

t

8

m m

9

o o

10

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13

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15

16

17

t t

o o

r r

: :

S S

T T

O O

P P

18

19

18

19

a0-1 : Page number a14-17 : Z motor drive status b14-17 : Optical-path change motor drive status

(CW, CCW, STOP (default)) (CW, CCW, STOP (default))

Optical path change, [a] : Drives the Z motor ([CW] → [STOP] → [CCW] → [STOP], upon each push) Optical path change, [d] : Drives the optical-path change motor ([CW] → [STOP] → [CCW] → [STOP], upon each push)

2-7

Page 7 -- Bottom and offset lens motor drives This page allows the operator to control the bottom motor drive and offset lens motor. 0

a P b

1

7

2

3

4

5

6

B

T O

M L

7

8

m m

9

o o

10

11

12

13

14

15

16

17

t t

o o

r r

: :

S S

T T

O O

P P

a0-1 : Page number a14-17 : Bottom drive status b14-17 : Offset lens motor drive status

(CW, CCW, STOP (default)) (CW, CCW, STOP (default))

Optical path change, [a] : Bottom drive ([CW]→[BREAK]→[CCW]→[BREAK] →[FREE]→[BREAK], upon each push) Optical path change, [d] : Offset lens motor drive ([CW] → [STOP] → [CCW] → [STOP], upon each push)

- 91 -

Appendix: Test Mode

R-4820.A

Test Mode 3.

Displaying Information About the Hub System The operator can display information about the motorized devices connected to the hub as the information is obtained via LVDS communication. It is not possible to control the motors of the motorized devices connected to the hub.

[Functions] • • • • • • • • • • • • • •

Motorized nosepiece connection status Motorized nosepiece address Condenser connection status Condenser address DM filter turret-1 connection status DM filter turret-1 address DM filter turret-2 connection status DM filter turret-2 address EX filter turret connection status EX filter turret address BA filter turret connection status BA filter turret address Mirror connection status Mirror address

• • • • •

Micrometer connection status Current micrometer address Setting of micrometer at power-on time Micrometer address at power-on time Micrometer limit status

• • • • • • • •

Motorized stage X-axis connection status Motorized stage X-axis position Motorized stage X-axis scale coordinate Motorized stage X-axis limit status Motorized stage Y-axis connection status Motorized stage Y-axis position Motorized stage Y-axis scale coordinate Motorized stage Y-axis limit status

• Shutter connection status • Shutter open/close status • Shutter maker • • • • •

DIA illumination operation selector DIA illumination status DIA illumination set voltage DIA illumination monitor voltage DIA illumination operation right - 92 -

Appendix: Test Mode

R-4820.A

Test Mode • • • • • •

Intensilight connection status Intensilight shutter open/close Intensilight ND Intensilight checksum Intensilight version Intensilight program name

• • • •

DSC #1usage status DSC #1use position DSC #2usage status DSC #2use position

• Ergonomic handle/knob Focus drive knob usage flag (=1: main-body knob; =2: ergonomic controller Z-axis knob) Focus drive knob coarse/fine speed (1to 7) Stage X-axis ergonomic controller handle usage flag (=0: not used; =1: X handle is used) Stage X-axis knob coarse/fine speed (1to 7) Stage Y-axis ergonomic controller handle usage flag (=0: not used; =1: Y handle is used) Stage Y-axis knob coarse/fine speed (1to 7) Micrometer ergonomic controller handle usage flag (=0: not used; =1: X handle is used; =2: Y handle is used) Offset lens external fine-motion handle usage flag (=0: not used; =1: external fine-motion handle is used) • • • • • • • • • • •

Motorized nosepiece PID information enable Motorized nosepiece PID information PfsR-P Motorized nosepiece PID information PfsR-I Motorized nosepiece PID information PfsR-D Motorized nosepiece PID information PfsR-T Motorized nosepiece PID information PfsR-V Motorized nosepiece PID information HubR-P Motorized nosepiece PID information HubR-I Motorized nosepiece PID information HubR-D Motorized nosepiece PID information HubR-T Motorized nosepiece PID information HubR-V

• • • • • • • •

Ergonomic controller connection information Ergonomic controller checksum Ergonomic controller version Ergonomic controller program name Joystick connection information Joystick checksum Joystick version Joystick program name - 93 -

Appendix: Test Mode

R-4820.A

Test Mode [Start/shutdown (special operation 2)] • Use the four optical path change switches located on the front of the main body. [a][a][d][d][b][c][b][c][b][b] Press the above key sequence within 10 seconds to display the "Hub system information page 1."

[Operation and page change] • On any page, use the four optical path change switches. [a]: Changes the value. (+) [b]: Changes the value. (-) [c]: Changes the page. (+) [d]: Changes the page. (-)

a b c

[Restrictions]

d • It is not possible to read any sensor connected to the hub. • Each switch does not have an inherent function, but its behavior varies with each page. Confirm the different behaviors as stated in the specification.

- 94 -

Appendix: Test Mode

R-4820.A

Test Mode 3-1

Page 1 -- Motorized accessories This page displays information about the motorized accessories of hub. 0

a P b

1

2

1

3

4

R E

E X

5

-

6

-

7

8

C B

O A

9

-

10

11

12

13

14

15

16

17

18

-

F M

1 I

-

-

F

2

-

-

19

a0-1 a5 a6 a9 a10 a13 a14 a17 a18 b5 b6 b9 b10 b13 b14

3-2

: : : : : : : : : : : : : : :

Page number Motorized nosepiece connection status Motorized nosepiece address Condenser connection status Condenser address DM filter turret-1 connection status DM filter turret-1 address DM filter turret-2 connection status DM filter turret-2 address EX filter turret connection status EX filter turret connection address BA filter turret connection status BA filter turret connection address Mirror connection status Mirror address

(=0: not connected; =1: connected) (=0:undefined; =1 to 6: address) (=0:not connected; =1: connected) (=0:undefined; =1 to 5: address) (=0:not connected; =1: connected) (=0:undefined; =1 to 6: address) (=0:not connected; =1: connected) (=0:undefined; =1 to 6: address) (=0:not connected; =1: connected) (=0:undefined; =1 to 8: address) (=0:not connected; =1: connected) (=0:undefined; =1 to 8: address) (=0:not connected; =1: connected) (=0:undefined; =1 to 2: address)

Page 2 -- Micrometer This page displays the status of the micrometer. 0

a P b

1

2

2

3

1

4

5

1

6

7

1

8

9

10

11

12

13

14

15

16

17

18

P A

o d

w d

: :

5 5

5 5

0 0

0 0

0 0

19

a0-1 a3 a5 a7 a14-18 b14-18

: : : : : :

Page number Micrometer connection status Setting status of micrometer at power-on time Micrometer limit status Micrometer address at power-on time Current micrometer address

- 95 -

(=0:not connected; =1: connected) (=0:not set, =1: already set) (=0:normal; =1:upper limit; =2:lower limit) (0 to 55,000) (0 to 55,000)

Appendix: Test Mode

R-4820.A

Test Mode 3-3 Page 3 -- Stage This page displays the status of the motorized stage. 0

a P b

1

2

3

3

X Y

4

1 1

5

1 1

6

-

7

5 3

8

5 7

9

0 0

10

11

12

13

14

15

16

17

18

19

0 0

0 0

0 0

-

5 3

5 7

0 0

0 0

0 0

0 0

a0-1 a4 a5 a6-12 a13-19 b4 b5 b6-12 b13-19

: Page number : Motorized stage X-axis connection status (=0: not connected; =1: connected; =2:connected (equipped with scale)) : Motorized stage X-axis limit status (=0:normal; =1:upper-limit; =2:lower-limit) : Motorized stage X-axis scale coordinate (-550,000 to 550,000) : Motorized stage X-axis scale coordinate (-550,000 to 550,000) : Motorized stage Y-axis connection status (=0:not connected、=1: connected、=2:connected (equipped with scale)) : Motorized stage Y-axis limit status (=0: normal; =1:upper-limit; =2:lower-limit) : Motorized stage Y-axis scale coordinate (-370,000 to 370,000) : Motorized stage Y-axis scale coordinate (-370,000 to70,000)

3-4 Page 4 -- Shutter This page displays the status of the shutter. 0

a p b

1

4

2

3

# #

4

1 2

5

-

6

1 1

7

8

0 0

9

: :

10

11

12

E E

P P

I I

13

14

15

16

17

18

1 1

: :

N N

I I

K K

19

a0-1 a6 a8 a14 b6 b8 b14

: : : : : : :

Page number Shutter #1 open/close status (=0: closed; =1: opened) Shutter #1 connection status (=0:not connected; =1:EPI、=2:DIA; =3:AUX) Shutter #1 maker (=0:not connected; =1:NIKON; =2:SHUTTER; =3:UNIBLITZ) Shutter #2 open/close status (=0: closed; =1: opened) Shutter #2 connection status (=0:not connected; =1:EPI; =2:DIA; =3:AUX) Shutter #2 maker (=0:not connected; =1:NIKON; =2:SHUTTER; =3:UNIBLITZ)

- 96 -

Appendix: Test Mode

R-4820.A

Test Mode 3-5

Page 5 -- DIA illumination This page displays the status of the DIA illumination. 0

a p b

1

2

5

3

4

E V

X :

5

T 2

6

: 5

7

8

1 5

9

10

11

12

13

14

O M

F :

F 1

: 0

0 2

4

15

16

17

18

O

:

1

19

a0-1 a7 a13 b5-7 b11-14 b18

3-6

: : : : : :

Page number DIA illumination operation selector (=0: EXTERNAL; =1: EXTERNAL (operable)) DIA illumination status (=0: unlit; =1: lit) DIA illumination set voltage (0 to 255) DIA illumination monitor voltage (0 to 1024) DIA illumination operation right (=0: not granted (TiE main-body side); =1 : granted (hub A/B side))

Page 6 -- Intensilight This page displays the status of the intensilight. 0

a p b

1

6

2

3

1 S

4

:

5

C 1

6

-

7

8

H N

G D

9

F :

10

11

I 1

E

12

13

14

15

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17

18

19

S

U

V M

1 :

. F

0 7

0 B

C

a0-1 a3 a5-12 a13-18 b5 b10 b16-19

: : : : : : :

Page number Intensilight connection status (=0:not connected; =1:connected) Intensilight program name (If not connected; [No Inten]) Intensilight version (If not connected; [siL--]) Intensilight shutter open/close (=0:closed; 01=:opened) Intensilight ND (=1:ND1; =2:ND2; =3:ND4; =4:ND8; =5:ND16; Intensilight checksum (If not connected; [----])

- 97 -

=6:ND32)

Appendix: Test Mode

R-4820.A

Test Mode 3-7

Page 7 -- DSC This page displays the status of the DSC. 0

a p b

1

2

7

3

# #

4

1 2

5

: :

6

1 1

7

: :

8

R R

9

o o

10

11

p p

12

13

14

15

16

3 3

: :

B B

o o

t t

17

18

19

18

19

a0-1 : a7 : a7 : a7 a7

3-8

: :

Page number DSC#1 usage status (=0: not used; =1: Roper; =2: NIKON) DSC#1 use position (=0: not used; =1:LEFT; =2: RIGHT; =3:BOTTOM; =4:FRONT; =5:BACK) DSC#2 usage status (=0: not used; =1: Roper; =2: NIKON) DSC#2 use position (=0: not used; =1:LEFT; =2:RIGHT; =3:BOTTOM; =4:FRONT; =5:BACK)

Page 8 -- Handle and knob data This page displays handle and knob data. 0

a p b

1

8

2

3

4

Z M

: M

5

1 :

6

0 0

7

8

X O

9

: L

10

11

1 :

0 0

12

13

14

15

16

Y

:

1

0

17

a0-1 : Page number a5 : Focus drive knob usage flag a6 a10

: :

a11 a15

: :

a16 b6

: :

b11

:

(=1: main-body knob; =2: ergonomic controller Z-axis knob) Focus drive knob coarse/fine speed (1to 7) Stage X-axis ergonomic controller handle usage flag (=0: not used; =1: X handle is used) Stage X-axis knob coarse/fine speed (1to 7) Stage X-axis ergonomic controller handle usage flag (=0: not used; =1: Y handle is used) Stage Y-axis knob coarse/fine speed (1to 7) Micrometer ergonomic controller handle usage flag (=0: not used; =1: X handle is used; =2: Y handle is used) Offset lens external fine-motion handle usage flag (=0: not used; =1: external fine-motion handle is used)

- 98 -

Appendix: Test Mode

R-4820.A

Test Mode 3-9

Page 9 -- PFS nosepiece PID This page displays PFS nosepiece PID parameters. 0

a p b P

1

9 :

2

F

3

P F

4

F F

5

6

1 F

7

T I

8

: :

9

F F

10

11

12

F F

F F

F F

13

14

15

16

17

18

19

V D

: :

F F

F F

F F

F F

a0-1 a5

: Page number : Motorized nosepiece PID information enable (=0: default values used; =1:custom values enabled) a9-12 : PFS motorized nosepiece PID information PFS nosepiece – time (0 to FFFF) a16-19 : PFS motorized nosepiece PID information PFS nosepiece – voltage (0 to FFFF) b2-5 : PFS motorized nosepiece PID information PFS nosepiece -P (0 to FFFF) b9-12 : PFS motorized nosepiece PID information PFS nosepiece -I (0 to FFFF) b16-19 : PFS motorized nosepiece PID information PFS nosepiece -D (0 to FFFF)

3-10 Page 10 -- Hub-side nosepiece PID This page displays hub-side nosepiece PID parameters. 0

1

a p b P

A :

2

F

3

4

H F

U F

5

1 F

6

7

T I

8

: :

9

F F

10

11

12

F F

F F

F F

13

14

15

16

17

18

19

V D

: :

F F

F F

F F

F F

a0-1 a5

: Page number : Motorized nosepiece PID information enable (=0: default values used; =1:custom values enabled) a9-12 : HUB motorized nosepiece PID information PFS nosepiece – time (0 to FFFF) a16-19 : HUB motorized nosepiece PID information PFS nosepiece – voltage (0 to FFFF) b2-5 : HUB motorized nosepiece PID information PFS nosepiece -P (0 to FFFF) b9-12 : HUB motorized nosepiece PID information PFS nosepiece -I (0 to FFFF) b16-19 : HUB motorized nosepiece PID information PFS nosepiece -D (0 to FFFF)

- 99 -

Appendix: Test Mode

R-4820.A

Test Mode 3-11

Page 11 -- Ergonomic controller and joystick information

This page displays information about the ergonomic controller and the joystick. 0

a E b J

1

1 1

2

3

* *

4

* *

5

n n

6

a a

7

m m

8

e e

9

* *

10

11

12

13

14

15

16

17

18

19

* *

V V

1 1

. .

0 0

0 0

F F

F F

F F

F F

a0-1 a13 a13 b18 a0-1 a13 a13 b18

: : : : : : : :

Ergonomic controller connection information Ergonomic controller program name Ergonomic controller version Ergonomic controller checksum Joystick connection information Joystick program name Joystick version Joystick checksum

- 100 -

(=0:not connected; =1: connected) (If not connected; [Ergo non]) (If not connected; [ conn]) (If not connected; [ect!]) (=0:not connected; =1: connected) (If not connected; [JoyS non]) (If not connected; [ conn]) (If not connected;、[ect!])

Appendix: Test Mode

R-4820.A

Test Mode 4.

Displaying/Editing PFS Parameters On the front VFD, the operator can display PFS parameters for the purpose of PFS debugging.

[Functions] • • • • • • • •

Displaying/editing PFS control speed UPPER Displaying/editing PFS control speed LOWER Displaying/editing PFS control speed OFFSET Displaying, and automatically starting to set, division border line Displaying/editing PFS control speed control gain Displaying/editing PFS offset zero position Displaying/operating IR LED DA value Displaying offset lens coordinate

a b c d

[Start (special operation 3)] • Use the four optical path change switches located on the front of the main body. [a][a][d][d][b][c][b][c][a][a] Press the above key sequence within 10 seconds to display the "Displaying/editing PFS parameters page 1."

[Shutdown] • Press the [BRIGHTNESS] button four times within 5 seconds.

[Operation and page change] • On any page, use the four optical path change switches. [a]: Changes the value. (+) [b]: Changes the value. (-) [c]: Changes the page. (+) [d]: Changes the page. (-)

[Restrictions] • Each switch does not have an inherent function, but its behavior varies with each page. Confirm the different behaviors as stated in the specification.

- 101 -

Appendix: Test Mode

R-4820.A

Test Mode 4-1 Page 1 -- Speed control parameters This page is used to adjust focusing speed such as when PFS is hunting. 0

a * b u

1

* :

2

* 0

3

*

4

* l

5

* :

6

* 0

7

*

8

* o

9

* :

10

11

12

13

14

15

16

17

18

19

*

P *

F *

S *

1 *

* 0

*

*

a0-10 a16-19 b2 b6 b10-11 b13-19

: : : : : :

Information about objective lens mounting Page number PFS control speed UPPER (0 to 9) PFS control speed LOWER (0 to 9) PFS control speed OFFSET (0 to 9) PFS on/off state

Each push of the [b] or [c] switch changes the controllable parameters. [a]SW [d]SW [a]SW [d]SW [a]SW [d]SW

4-2

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PFS control speed UPPER increment PFS control speed UPPER decrement PFS control speed LOWER increment PFS control speed LOWER decrement PFS control speed OFFSET increment PFS control speed OFFSET decrement

Page 2 -- Control speed gain This page is used to adjust the PFS objective lens parameters (for debugging by design personnel). 0

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Information about objective lens mounting Page number PFS control speed GAIN (0 to 9990) PFS on/off state

[a]SW : PFS control speed GAIN increment [d]SW : PFS control speed GAIN decrement - 102 -

Appendix: Test Mode

R-4820.A

Test Mode 4-3

Page 3 -- Automatic adjustment division boundary line This page is used for automatically adjusting the division boundary line of the optical system (CMOS) of PFS. 0

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a4-6 : Division boundary line (value) a16-19 : Page number

[a]SW : Starts/pauses automatic setting [d]SW : Moves the offset lens to the zero-offset position. Setting procedure: (1) Mount the objective lens "MRH00401 Plan Flour 40x" in the nosepiece and move it into the optical path. (2) As a specimen, place a glass bottom dish (filled with water) on the stage. (3) Press the [d] switch until the offset lens reaches the position "offset = 0". (The "offset = 0" adjustment must have been completed before starting this adjustment procedure.) (4) Using the manual handle of the microscope, move the tip of the objective lens at least 3 mm away from surface "A" of the glass bottom dish. Surface "B"

Glass bottom dish

Water ≤ 3mm Surface "A"

Animation is displayed on the LCD in the course of these steps.

MRH00401 Plan Flour 40x

(5) (6)

Press the [a] switch. (A short beep sounds.) Move the TE2000E's focus drive in fine mode. • Perform pre-adjustment, adjusting the LED light intensity and executing coarse search of focus drive position. • A short beep sounds continually during the pre-adjustment. (7) Back to the adjustment procedure, execute fine search of focus drive position. • A short beep followed by silence sounds continually during the adjustment procedure. (8) The fine search finishes after while, when the beeping stops. (9) After 10 seconds, a long beep sounds and the "POWER" LED lights. This completes the setting procedure. CAUTION: Leave the focusing knob intact during steps (6) and (8). - 103 -

Appendix: Test Mode

R-4820.A

Test Mode 4-4

Page 4 -- Offset zero-position setting This page is used for automatically adjusting the division boundary line of the optical system (CMOS) of PFS. 0

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Coordinate of offset lens (approx. 0 to 35000; variable with each lens) Coordinate of offset zero position (approx. 2000; varies with each individual unit) Page number Set value of IR LED DA (0 to 4096) Exposure time adjustment (1, 4, 16, 32) PFS on/off state

(1) If an asterisk "*" is shown to the left of "L": [a]SW : Increments IR LED DA by four. [d]SW : Decrements IR LED DA by four. (2) If an asterisk "*" is shown to the left of "O": [a]SW : The offset lens is ready to drive. [d]SW : Set the offset zero position and write it into EEPROM (the same effect as by the communication command [cYEE4]).

NOTE: (1) Exposure time (ND) adjustment Use the [Z-RESET] key to adjust exposure time (ND). Increasing the ND number reduces the CMOS exposure time and lowers the level of AF signal of PFS (the signal can be observed on an oscilloscope). → Use this control if the AF signal is saturated even when the LED output indication (b-3 to b-6) is "0." (2) Optical-path change (EYE100) When an asterisk "*" is shown to the left of "O", pressing [R100] (=[c]) sets the optical path to EYE100.

- 104 -

Appendix: Test Mode

R-4820.A

Test Mode 5.

Displaying/Editing Software DIP Switches The parameters listed below can be enabled/disabled or changed for the purpose of main-body debugging.

[Functions] • • • • • • • • • •

Disable AF_OFF upon error occurrence No AF time-out Disable offset lens software limit a Enable set cLEG value Keep offset lens movable b RS232C CCD data log output Lv1 RS232C CCD data log output Lv2 RS232C CCD data log output Lv3 Activate focus drive during AF LED auto gain control Turn off IR LED when in wait state

c d

[Start (special operation 4)] • Use the four optical path change switches located on the front of the main body. [a][a][d][d][b][c][b][c][d][d] Press the above key sequence within 10 seconds to display the "Displaying/editing software DIP switchs page 1."

[Shutdown] • Press the [BRIGHTNESS] button four times within 5 seconds.

[Operation and page change] • On any page, use the four optical path change switches. [a]: Changes the value. (+) [b]: Changes the value. (-) [c]: Changes the page. (+) [d]: Changes the page. (-)

[Restrictions] • Each switch does not have an inherent function, but its behavior varies with each page. Confirm the different behaviors as stated in the specification.

- 105 -

Appendix: Test Mode

R-4820.A

Test Mode 5-1 Page 1 This page is used to enable/disable or changed parameters. 0

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Information about objective lens mounting PFS on/off state Disable AF_OFF upon error occurrence No AF time-out Disable offset lens software limit Enable set cLEG value Keep offset lens movable RS232C CCD data log output Lv1 RS232C CCD data log output Lv2 RS232C CCD data log output Lv3 Activate focus drive during AF LED auto gain control Turn off IR LED when in wait state IR LED auto gain set one at a time Change focus LED lighting conditions Spare Spare Spare Spare

Each push of the [b] or [c] switch changes the controllable parameters. [a]SW [d]SW

: Increments the parameter. : Decrements the parameter.

- 106 -

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Appendix: Test Mode

R-4820.A

Test Mode 6.

Displaying/Editing PFS Parameters (for supporting sold products) On the front VFD, the operator can display PFS parameters for the purpose of supporting sold PFS units.

[Functions] • Display/edit PFS speed control OFFSET • Save (in EEPROM)

[Start (special operation 5)] • Use the four optical path change switches and [Brightness] switch located on the front of the main body. [a][a][d][d][b][c][b][c][Brightness][Brightness] Press the above key sequence within 10 seconds to display the "Displaying/editing PFS parameters page 1."

a b c d [Shutdown] • Press the [BRIGHTNESS] button four times within 5 seconds.

[Restrictions] • Each switch does not have an inherent function. Check with the specification before use.

- 107 -

Appendix: Test Mode

R-4820.A

Test Mode 6-1 Page 1 This page is used to adjust focusing speed such as when PFS is hunting. 0

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Information about objective lens mounting PFS on/off state PFS control speed (Pfs Gain : -9 to 9) Set value of IR LED DA (0 to 4096) Save (in EEPROM)

Each push of the [b] or [c] switch changes the controllable parameters, display an underbar at the corresponding position. (1)

Underbar at "PfG" [a]SW : Increments PFS control speed by one. [d]SW : Decrements PFS control speed by one.

(2)

Underbar at "L" [a]SW : Increments IRLED DA by four. [d]SW : Decrements IRLED DA by four.

(3)

Underbar at "SAVE" [d]SW : Executes save.

- 108 -