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HPLC HPL C Column Section HPLC Column Comparison ................................................................................. 350-351 Alltech HPLC Column Packing Characteristics .................................................... 352-353 HPLC Column Formats ............................................................................................. 354 Standard HPLC Column Formats ........................................................................... 355 Rocket™ ........................................................................................................ 356-357 LC/MS ................................................................................................................. 358 Stainless Steel Preparative ................................................................................... 359 Custom HPLC Columns ....................................................................... 355, 356, 359-361 Alltech HPLC Columns Adsorbosil™ .......................................................................................................... 362 Adsorbosphere .............................................................................................. 363-365 Allsphere™ ........................................................................................................... 366 Alltima™ ....................................................................................................... 367-375 Alltima™ Narrow-Bore ......................................................................................... 371 Apollo .......................................................................................................... 376-377 alphaBond™ ......................................................................................................... 378 Carbohydrate ....................................................................................................... 379 Econosil™ ............................................................................................................. 380 Econosphere™ ................................................................................................ 381-382 Macrosphere™ ............................................................................................... 383-384 Mixed-Mode™ ....................................................................................................... 385 Organic Acid ........................................................................................................ 386 Platinum™ ..................................................................................................... 387-392 Prevail™ ........................................................................................................ 393-402 Prosphere™ 300 ................................................................................................... 403 AKZ0 Nobel Kromasil™ .......................................................................................... 404 Astec ............................................................................................................ 405-407

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ZORBAX® ....................................................................................................... 445-451

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ZirChrom® ..................................................................................................... 443-444

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Whatman® ........................................................................................................... 442

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Waters® Spherisorb® ............................................................................................ 441

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Shodex® ............................................................................................................... 440

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Merck ........................................................................................................... 438-439

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Macherey-Nagel ................................................................................................... 437

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Jordi ............................................................................................................ 431-436

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Hypersil® ...................................................................................................... 420-430

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Hamilton ............................................................................................................. 419

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GL Science Inertsil® ....................................................................................... 412-418

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Brownlee™ .................................................................................................... 409-411

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Beckman ............................................................................................................. 408

HPLC Column Comparison HPLC Column Selection: A Comparison of Alltech C18 and C8 Columns Alltech has developed a method for choosing C18 and C8 columns based on peak capacities and column selectivity of polar and nonpolar compounds. Typically chromatographers must choose HPLC columns by comparing the surface area and carbon load data supplied by the column manufacturers. This data does not provide enough information about column selectivity or capacity for adequate column selection. The charts Alltech has assembled provide a reliable means of choosing HPLC columns, whether for back-up columns having similar selectivity or for columns with very different selectivity.

Chromatographic Conditions and Calculations The test mix used for this comparison contains uracil as a void volume marker, pyridine and N,N-dimethylaniline as basic probes, 4-butylbenzoic acid and phenol as acidic probes, and toluene as a neutral probe. Toluene tests the hydrophobic character of the columns, and the polar components test the activity of the base silica. The mobile phase contains 62% acetonitrile and 38% phosphate buffer at pH 3.2, which protonates the 4-butylbenzoic acid and insures consistent retention times for the amine compounds.

Directions for Using the Column Chart The chart lists the columns in descending order of toluene capacity. To find similar HPLC columns to test as back-up columns, follow these 4 steps. 1) Find the column you are currently using and note neighboring columns which have similar toluene capacity factors. 2) Compare the peak order and capacity factors for the polar compounds. 3) Check whether any of the compounds tail, especially pyridine, N,N-dimethylaniline, and 4-butylbenzoic acid. Any peak with a tailing factor greater than 2.0 is listed as a tailing peak. 4) Compare uracil void times to insure similar retention times. If there is more than one choice for a back-up column, then compare your actual sample to the test probes. If your sample is nonpolar like toluene, then place more emphasis on the toluene capacity. If your sample is a polar amine sample, then pay special attention to the pyridine and N,N-dimethylaniline probes. If your sample is an acid, note the activity of the 4-butylbenzoic acid and phenol probes.

Capacity factor (k’) was calculated using k’ = (tr - to)/to, where tr is the retention time of the peak of interest and to is the uracil void time. The tailing factor was calculated using the USP formula T = W0.05/2f.

Adsorbosphere UHS C18 Inertsil® ODS-3 Apollo C18 Alltima™ C18 Kromasil™ C18 Luna™ C18 Columbus™ C18 LiChrospher® RP-18 Brownlee Spheri™-5 RP-18 LiChrospher RP®-18 (endcapped) Inertsil® ODS-2 Adsorbosil® C18 Econosil™ C18 Brownlee Spheri-5™ ODS Prevail C18 Prodigy™ ODS (3) Symmetry® C18 Luna™ C8 Adsorbosphere® HS C18 Zorbax® ODS AquaSep™ AQS C8 Waters Spherisorb® ODS-2 Ultrasphere® C18 YMC ODS-A Microsorb™-MV C18 Nucleosil® C18 AB

P L L P L P P L L P P L L P PL PL L P L P L P L P L P PL L P L P L P P L LP PL PL P L L P P L P L 1 0

P — Pyridine L — Phenol N — N,N-Dimethylaniline B — 4-Butylbenzoic Acid T — Toluene

N=4.52 B=4.53 T=6.94 B T=4.55 N N B T=4.50 T=4.44 B N T=4.27 B N B N T=4.23 B N T=4.10 T N B T B N T N B T B N T B N T B N T B N N BT T B N T N B T B N T B N T B N T B N T NB T B N T N B T N B=18.71 T N B 2 3 4 Capacity Factor

URACIL VOID TIME (MIN) 1.04 1.39 1.18 1.19 1.17 1.28 1.35 1.30 1.22 1.30 1.28 1.27 1.26 1.25 1.24 1.31 1.20 1.29 1.38 1.20 1.46 1.16 1.22 1.41 1.23 1.25

TAILING PEAKS

P P, B

P P P, N, B

P P, B P P, B P, B P, B P, B

SEE PAGE 368 412 378 371 404 N/A N/A 439 410 439 412 367 381 410 393 N/A N/A N/A 368 N/A N/A 441 408 N/A N/A 437

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Key Chart

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Continued on next page

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© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

HPLC Column Comparison

B

P, B

B B

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B

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B

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P, B

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P, N, B P, N, B P, N, B

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B P

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P P

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B=14.04

P, B P, B

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B=7.31

B

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P=4.14

B B

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B=7.22

P, N B

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B

P, B P, N, B P, N, B B P, B

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B=4.47

P, B

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B=7.10

P, B B

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B

P, N P B

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N

P P, N, B P, N, B P, B B P

SEE PAGE N/A N/A 404 369 370 408 442 N/A 438 368 437 N/A N/A 410 438 371 N/A N/A 371 408 N/A N/A 424 442 N/A 377 382 441 420 412 442 N/A N/A N/A 370 N/A 441 369 370 N/A 387 367 381 N/A N/A 441 420 368 424 424 437 N/A 382 393 N/A 438 N/A N/A 437 387 441 387 437 369 N/A N/A N/A 442 369 403 N/A 384 442 430 N/A N/A 387 369 384 403 369

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T T T T T T T T BT T T B

TAILING PEAKS P, N

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P P

L N B L N B N LP B N LP B P L N B N L P LP NB P L N B P L B P L N B P L N B P L N B P L N P L N P L N B PL NB P L N BT P T L N B P T L N B P L T NB P T L NB P T L NB P T L N B P L T N B P L T N B N P T L B PL T N P T L N B P T L N B P T L N B P T L N P T L N B P L T N B N P T B L PL T N B NB T P L PL T NB PL T NB P T L N P L T N B T L P BN T L P BN P T L N B P T L N N P T L B T P L N B L P B N T L B TN NB T P L T LP NB PL N BT LP BN T N BT PL P L N B T NB T P L T P L N B P L N B T PL NB T NB T P L PL N BT L P N B T PL NB T PL NB T PL N BT PL N BT P L NB T P L NB T PL N T PL N BT T N PL B PL NB T L P NB T P L N BT P L NB T PL NB T NT L PB P L NB T PL N BT PLNB T PL N T

URACIL VOID TIME (MIN) 1.36 1.48 1.31 1.22 1.20 1.18 1.38 1.43 1.46 1.29 1.45 1.31 1.22 1.29 1.32 1.35 1.15 1.40 1.36 1.33 1.18 1.40 1.41 1.51 1.42 1.58 1.36 1.25 1.37 1.50 1.51 1.52 1.45 1.63 1.26 1.31 1.31 1.34 1.33 1.48 1.38 1.46 1.45 1.69 1.39 1.32 1.45 1.40 1.49 1.46 1.53 1.51 1.42 1.45 1.67 1.65 1.68 1.77 1.69 1.44 1.79 1.48 1.67 1.68 1.55 1.46 1.88 1.60 1.43 1.68 1.67 1.62 1.75 1.73 1.58 1.59 1.58 1.78 1.81 1.80 1.59

NT T T T T T

P B

L

hplc hplc

Resolve“ C18 YMC ODS-AQ Kromasil“ C8 Adsorbosphere¤ XL C18 Allsphere“ ODS-2 Ultrasphere¤ C18-IP Partisil¤ ODS-2 Versapack“ C18 LiChrosorb¤ RP-18 Adsorbosphere¤ C18 Nucleosil¤ C18 Symmetry¤ C8 Zorbax¤ SB-C18 Brownlee Spheri“-5 RP-8 LiChrosorb¤ RP Select B Alltima“ C18-LL Zorbax¤ Rx-C18 Supelcosil“ LC-18-DB Alltima“ C8 Ultrasphere¤ C8 Nova-Pak¤ C18 Pinnacle“ C18 Hypersil¤ ODS Partisil¤ ODS-3 SynChropak¤ SCD-100 alphaBond“ C18 Econosphere“ C18 Waters Spherisorb¤ ODS-B Hypersil¤ BDS C18 Inertsil¤ C8 Partisil¤ C8 Prism¤ RP SynChropak¤ RPP-100 (C18) Bondapak¤ C18 Allsphere“ ODS-1 Zorbax¤ SB C8 Waters Spherisorb¤ ODS-1 Adsorbosphere¤ XL C8 Allsphere“ Octyl Supelcosil“ ABZ+ Platinum“ C18 Adsorbosil¤ C8 Econosil“ C8 HyPURITY“ Elite C18 Suplex pKb-100 Waters Spherisorb¤ Octyl Hypersil¤ BDS C8 Adsorbosphere¤ C8 Hypersil¤ MOS-2 C8 Hypersil¤ MOS-1 C8 Nucleosil¤ C8 Vydac¤ 201HS, C18 Econosphere“ C8 Prevail C8 YMC Basic LiChrosorb¤ RP-8 Prism¤ RPN Jupiter“ C18 Nucleosil¤ C18 (300 ) Platinum“ C8 Waters Spherisorb¤ Wide Pore C18 Platinum“ EPS C18 Nucleosil¤ C8 (300 ) Adsorbosphere¤ XL C18 (300 ) Vydac¤ 201TP, C18 Vydac¤ 218TP, C18 Jupiter“ C5 Partisphere¤ C18 Adsorbosphere¤ XL C18-B ProSphere C18 (300 ) Deltabond¤ ODS Macrosphere“ C18 Partisil¤ ODS Hypersil¤ WP MOS C8 Vydac¤ 208TP, C8 SynChropak¤ RPP (C18, 300 ) Platinum“ EPS C8 Adsorbosphere¤ XL C8 (300 ) Macrosphere“ C8 ProSphere C4 (300 ) Adsorbosphere¤ XL C8-B

Contact your Alltech office or distributor for current or local prices.

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351

Alltech HPLC Column Packing Characteristics Packing Characteristics Reference Guide

[email protected] to help you choose an appropriate column for your application. In the US our technical specialists can be reached by phone at 1-800-33SOLVE Monday through Friday from 7:30am to 5:30pm (central time zone). The USP L-code listing lets you know that these columns meet the specifications set forth in USP Volume 24.

The table below lists the physical characteristics of the most common Alltech HPLC packings. This table is provided as a tool to help you make informed decisions regarding Alltech HPLC columns. Please note that Alltech HPLC technical specialists are available by email at PACKING

BASE MATERIAL

PARTICLE SHAPE

PARTICLE SIZE (µM)

CARBON LOAD (%)

PORE SIZE (Å)

SURFACE AREA (M2/G)

PHASE TYPE

CAPPED?

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Irregular Irregular Irregular Irregular Irregular Irregular

5, 10 5, 10 5, 10 5, 10 5, 10 5, 10

15% — 10% — — —

60 60 60 60 60 60

450 450 450 450 450 450

Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric

Yes — Yes Yes No —

L1 L16 L7 L10 L8 L3

Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical

3, 5, 10 3, 5, 10 3, 5, 10 5 3, 5, 10 5 5 5 3, 5, 10 3, 5, 7 3, 5, 7 10 5, 10 5, 10

12% 8% — — — — — — — 20% — 30% 5% —

80 80 80 120 80 80 80 80 80 60 60 60 60 80

200 200 200 170 200 200 200 200 200 350 350 500 200 200

Monomeric Monomeric Monomeric Polymeric Polymeric Monomeric Monomeric Monomeric — Monomeric — Monomeric Monomeric Monomeric

Yes Yes Yes No No Partial — Yes — Yes — Yes Yes —

L1 L7 L10 L10 L8 L18 — — L3 L1 L3 L1 L11 L13

Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical

3, 5,10 3, 5,10 3, 5,10 5,10 3, 5 3, 5,10 3, 5,10 3, 5,10 5,10 5,10

11% 12% 6% — — — — — — —

90 90 90 90 90 90 90 90 90 90

200 200 200 200 200 200 200 200 200 200

Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric — Monomeric Monomeric

Yes Yes Yes Yes Yes Yes No — Yes Yes

L1 L1 L7 L13 L11 L10 L8 L3 — —

Spherical Spherical

3, 5,10 3, 5,10

6% 3%

300 300

100 100

Monomeric Monomeric

Yes Yes

L1 L7

Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical

3, 5, 10 5, 10 3, 5, 10 5 5, 10 3, 5, 10 3, 5, 10 5, 10 5 5 3, 5, 10

12% 7% 6% 4% 3% 3.5% 3% 3% 4% 4% —

80 80 80 80 80 80 80 80 100 100 80

220 220 220 220 220 220 220 220 220 220 220

Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric —

Yes Partial Yes Yes Yes No No No No No —

L1 L1 L7 L15 L11 L10 L8 L13 — — L3

Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical

3,5 3, 5, 10 5 3, 5, 10 3, 5, 10 3, 5, 10 3, 5 3, 5

15% 16% 9% 9% 7.5% — — —

100 100 100 100 100 100 100 100

— — — — — — — —

Polymeric Polymeric Polymeric Polymeric Polymeric — Polymeric Polymeric

Yes Yes Yes Yes Yes — No Yes

L1 L1 L1 L7 L11 L3 L8 L10

Spherical Spherical

5 5

17% 6.5%

200 200

– –

Polymeric Polymeric

Yes Yes

L1 L26

Irregular Irregular Irregular

5, 10 10 10

10% — —

125 125 125

300 300 300

Monomeric Monomeric Monomeric

Yes Yes Yes

L1 L7 L11

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Alltech HPLC Column Packing Characteristics

352

Adsorbosil® C18 Silica C2 Silica C8 Silica CN Silica Silica NH2 Silica Silica Adsorbosphere® C18 Silica C8 Silica CN Silica CN-AQ Silica NH2 Silica PAC Silica SAX Silica SCX Silica Silica Silica HS C18 Silica HS Silica Silica UHS C18 Silica Phenyl Silica TMS Silica Adsorbosphere® XL C18 Silica C18-B Silica C8 Silica C1 Silica Phenyl Silica CN Silica Silica NH2 Silica Silica SAX Silica SCX Silica ® Adsorbosphere XL 300 C18 Silica C8 Silica Allsphere™ ODS-2 Silica ODS-1 Silica C8 Silica C6 Silica Phenyl Silica CN Silica NH2 Silica C1 Silica SAX Silica SCX Silica Silica Silica ™ Alltima AQ Silica C18 Silica C18 LL Silica C8 Silica Phenyl Silica Silica Silica NH2 Silica CN Silica ™ Alltima WP C18 Silica C4 Silica alphaBond™ C18 Silica C8 Silica Phenyl Silica

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech HPLC Column Packing Characteristics PACKING

BASE MATERIAL

PARTICLE SHAPE

PARTICLE SIZE (µM)

CARBON LOAD (%)

PORE SIZE (Å)

SURFACE AREA (M2/G)

PHASE TYPE

CAPPED?

END-

USP L-CODE

Alltech HPLC Column Packing Characteristics 10 10 5, 10

— — —

125 125 125

300 300 300

Monomeric Polymeric —

Yes No —

L10 L8 L3

Silica Silica Silica

Spherical Spherical Spherical

5 5 5

15% 8% —

100 100 100

340 340 340

— — —

Yes Yes —

L1 L11 L3

Silica Silica

Spherical Irregular

7 10

— —

100 80

350 550

Polymeric Polymeric

No No

— —

Silica Silica Silica Silica Silica

Irregular Irregular Irregular Irregular Irregular

5,10 5, 10 5, 10 5, 10 5, 10

15% 10% — — —

60 60 60 60 60

450 450 450 450 450

Polymeric Polymeric Polymeric Polymeric —

Yes Yes Yes No —

L1 L7 L10 L8 L3

Silica Silica Silica Silica Silica

Spherical Spherical Spherical Spherical Spherical

3, 5, 10 3, 5, 10 5, 10 5, 10 3, 5, 10

10% 5% — — —

80 80 80 80 80

200 200 200 200 200

Monomeric Monomeric Monomeric Polymeric —

Yes Yes Yes No —

L1 L7 L10 L8 L3

Silica Silica Silica Silica Silica Silica Silica

Spherical Spherical Spherical Spherical Spherical Spherical Spherical

7 7 7 7 7 7 7

— — — — — — —

60 100 150 300 500 1000 4000

450 350 200 100 35 25 10

Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric Polymeric

No No No No No No No

L25 — — — — — —

Silica Silica Silica Silica Silica Silica Silica

Spherical Spherical Spherical Spherical Spherical Spherical Spherical

5, 7 5, 7 5, 7 7 7 7 7

10% 2.2% — — — — —

300 300 300 300 300 300 300

100 100 100 100 100 100 100

Monomeric Monomeric Monomeric Monomeric Polymeric Monomeric Polymeric

Yes Yes Yes No No No No

L1 L7 L26 — — — —

Silica Silica Silica

Spherical Spherical Spherical

7 5 5, 7

— — —

100 100 100

350 350 350

Polymeric Polymeric Polymeric

No No No

— — —

Silica Silica Silica Silica Silica Silica Silica Silica Silica Silica

Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical

1.5, 3, 5, 10 5, 10 3, 5, 10 5, 10 5, 10 3, 5, 10 3, 5, 10 3, 5, 10 3, 5 3, 5, 10

6% — 4% — — — — — — —

100 300 100 300 300 100 100 100 100 100

200 100 200 100 100 200 200 200 200 200

Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric —

Yes Yes Yes Yes Yes Yes No No No —

L1 L1 L7 L7 L26 L11 L10 L8 — L3

Silica Silica

Spherical Spherical

1.5, 3, 5, 10 3, 5, 10

5% 2.5%

100 100

200 200

Monomeric Monomeric

No No

L1 L7

Silica Silica Silica Silica Silica Silica Silica Silica

Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical

3, 5 3, 5 3, 5 3, 5 3, 5 3, 5 3, 5 3, 5

15% 8% 7% — — — — —

110 110 110 110 110 110 110 110

350 350 350 350 350 350 350 350

Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric Monomeric

Yes Yes Yes Yes No — No Yes

L1 L7 L11 L10 L8 L3 — —

Silica Silica Silica

Spherical Spherical Spherical

5, 10 5 5, 10

9% 15% 3%

300 100 300

100 350 100

Polymeric Monomeric Polymeric

Yes Yes Yes

L1 L1 L26

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Irregular Irregular Irregular

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© Copyright 2003 Alltech Associates, Inc.

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Silica Silica Silica

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alphaBond™ (cont.) CN NH2 Silica Apollo C18 Phenyl Silica Carbohydrate Anomer Analysis Amino Phase Econosil™ C18 C8 CN NH2 Silica Econosphere™ C18 C8 CN NH2 Silica Macrosphere™ GPC GPC 60 GPC 100 GPC 150 GPC 300 GPC 500 GPC 1000 GPC 4000 Macrosphere™ 300 C18 C8 C4 WAX WCX SAX SCX Mixed Mode™ C8/Anion C8/Cation C18/Cation Platinum™ C18 C18-300 C8 C8-300 C4-300 Phenyl CN NH2 SAX Silica Platinum™ EPS EPS C18 EPS C8 Prevail™ C18 C8 Phenyl CN NH2 Silica Amide Organic Acid Prosphere™ C18-300 C18-100 C4

353

HPLC Column Formats – Introduction Standard Analytical Columns – Metal-Free

Choosing the Right HPLC Column Format Packing material, column dimensions and mobile phase are the most important tools you have to control your HPLC separation. But don't forget that column format can further enhance your chromatography, or offer necessary convenience. Alltech offers seven column formats, each with many advantages. Stainless steel formats are appropriate for all separations except those with metal-sensitive samples or mobile phases that corrode stainless steel. Use a metal-free format for these applications.

Alltech Stainless Steel Columns Are The Best! No matter what HPLC packing you use, our exclusive column hardware improves performance and makes your work easier. And when you choose any Alltech Stainless Steel Column, you get these exclusive features: Increased efficiency and reproducibility Your methods depend on sharp, symmetrical peaks; day-to-day, lab-to-lab and column-to-column. Alltech stainless steel columns help you achieve these goals. Our ultra-smooth column tubing makes Alltech HPLC columns more efficient and consistent .

4730

Durable, Convenient Endfittings - Our stainless steel endfittings are virtually indestructible, and readily accept stainless steel or PEEK connecting tubing and fittings. Plus, changing frits has never been easier. Frit assemblies (standard analytical columns) or pistons (CAP™ columns and Rocket™ columns) never stick in the end fitting, and align automatically for a perfect fit every time. And reusable cartridge end fittings include a built-in prefilter that may be replaced in seconds without tools. Choose from four different stainless steel formats:

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Metal-free columns are found in the IC Section, see pages 538-554. Also available as custom columns, see page 355.

Expedite™ Columns for LC/MS Expedite your LC/MS analyses with Alltech’s high speed, low volume Expedite™ hardware. Especially designed for high throughput applications that employ mass spec detectors, they are the newest of Alltech’s wide selection of column formats for LC/MS. See page 358 for details.

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Expedite™ hardware available in 3 Alltech packing materials, see page 358.

4731

Built for high throughput and high speed, methods run 3 to 5 times faster on Rocket columns than on conventional columns, with equal or better resolution. Rocket columns are available as stock items with most popular packing materials. See page 356 for details.

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3806

Rocket™ hardware is available packed with most Alltech packing materials, see pages 356-357. Also available as custom columns, see page 356.

Preparative Columns

5319

Alltech analytical columns include seven durable, reusable, interchangeable, color-coded bands that quickly and easily snapfit onto the end fittings. Use color alone as an indicator, or add text by writing in the band's outer surface. Identify column inlet and outlet, storage solvent, method, owner, age, or just about any other information.

Our preparative column design features a threaded column tube format (Type 316 Stainless Steel) with stainless steel endfittings and PEEK pistons. Preparative columns 5544 are used when higher volume and mass loading capacities are needed for isolation and purification procedures. Alltech preparative columns are available in 7, 10, and 22mm ID formats. Larger ID columns are available upon request.

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Preparative columns are available in 8 different Alltech packing materials, see page 359. Also available as custom columns, see page 359.

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CAP (Piston) and cartridge formats are available as custom columns; request data sheet 75066D.

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For packing materials available in standard stainless steel analytical formats, see pages 362-451. Also available as custom columns, see page 355.

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5545

High-Speed Rocket™ Columns

Standard Analytical Columns – Stainless Steel Stainless steel analytical columns are the perfect match for most routine work. Columns in this format are available as stock items with most popular packing materials. Choose from two end fitting options: one configured for the industry standard compression screws and ferrules, and the second for compression screws and ferrules supplied by Waters. See page 355 for details.

PEEK-lined metal-free analytical columns combine the inertness of PEEK and the strength of stainless steel. Use these columns for biological or ion chromatography applications. The columns are listed in the IC section or can be ordered as custom.

354

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Standard HPLC Column Formats Stainless Steel Columns in a Wide Range of Internal Diameters and Lengths

Stainless Steel Analytical Column Specifications Material: Max. Temperature: Max. Pressure: Thread Type: Typical Use:

Colored Column Band

Highly Polished Threaded Column Tube

316 SS, PEEK 120° C 10,000psig 10-32 UNF (Industry Standard Ports) Analytical HPLC Applications

Replacement Endfittings and Colored Bands

Frit/Insert

Stainless Steel Endfitting

Figure 1

5319

Alltech’s stainless steel analytical columns use a highly polished 5/16" OD Type 316 stainless steel threaded tube, stainless steel female endfittings, and a frit/insert made from stainless steel and PEEK (Figure 1). Alltech columns include seven reusable, interchangeable, colorcoded bands that quickly and easily snap-fit onto the column endfittings. Use color alone as an indicator, or add text by writing on the band’s outer surface. Every column comes with seven bands, one each in six colors (green, red, orange, white, blue, and yellow), plus a second green band attached to the column inlet. Two frit/insert types are available: one to accept industry standard compression screws and ferrules, and another to accept connecting tubing configured with compression screws and ferrules from Waters Corporation. Almost all commercially available HPLC columns use industry standard endfittings. The two frit/insert types differ only in the distance the connecting tubes penetrate into the endfitting. The distance is known as the “stop depth”. The stop depth on the connecting tubing must match the stop depth in the insert. A mismatch will either create dead volume or cause a leak (Figure 2). This is only an issue with stainless steel ferrules that seat onto the tubing. Alltech recommends you make it a non-issue by using PEEK high pressure fittings and ferrules. The PEEK ferrules adjust to the exact stop depth with each connection. See pages 467-472 for Alltech’s complete PEEK fitting offering. For information on metal-free hardware, see page 455. Endfitting Ferrule

QTY

PART NO.

Endfittings and Colored Bands SS Endfitting for 2.1-4.6mm ID SS Endfitting for 1.0mm ID Assortment of 6 Colored Bands Green Band Orange Band Blue Band White Band Yellow Band Red Band

Ea Ea 6 10 10 10 10 10 10

75066 60013 93237 93231 93232 93233 93234 93235 93236

PRICE

Replacement Frit/Inserts COLUMN ID

POROSITY

QTY

Frit/Inserts 4.6mm 2µm 4.6mm 0.5µm 4.1mm 2µm 4.1mm 0.5µm 3.9mm 2µm 3.9mm 0.5µm 3.2mm 2µm 3.2mm 0.5µm 2.1mm 2µm 2.1mm 0.5µm

10 10 10 10 10 10 10 10 10 10

INDUSTRY STANDARD WATERS INSTRUMENT PART NO. PART NO.

75080 75081 75082 75083 75084 75085 75086 75087 75088 75089

PRICE

75050 75051 75052 75053 75054 75055 75056 75057 75058 75059

Custom Analytical Column Ordering Instructions Stainless steel columns can be custom manufactured with any of the packing materials on page 360-361. To determine the price for your column, follow this simple procedure: 1. Find your material of choice in the packing media listing on page 360-361. Identify the price code for the desired particle size. 2. Choose your column length and internal diameter. 3. Match up your media price code with the dimensions of your column using the chart below to determine the cost of your custom column.

hplc hplc

Stop Depth (0.100" for Industry Std., 0.125" for Waters Instr.)

DESCRIPTION

SS Analytical Custom Column Options ○ ○ ○ ○

Leak

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C-6000B for Industry Standard Endfittings C-6000C for Waters Instrument Endfittings C-6000M for PEEK-lined Hardware* Internal Diameter: 1.0-**, 2.1-, 3.2-, 3.9-, 4.1- or 4.6mm Length: 30-, 50-, 100-, 150-, 250- or 300mm Packing Material: Name and Particle Size

Frit

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MEDIA PRICE CODE

1

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COLUMN LENGTH

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30, 50mm 100, 150mm 250, 300mm

© Copyright 2003 Alltech Associates, Inc.

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Figure 2 - Problems that Arise from Mismatched Stop Depth Contact your Alltech office or distributor for current or local prices.

Stainless Steel Column Prices

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0630

Leaks are created when you plumb an industry standard column with tubing swaged for a Waters instrument port.

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0629

Dead Volume is created when you plumb a Waters Column with tubing swaged for an industry standard port.

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* PEEK-lined hardware is available with internal diameters of 2.1 and 4.6mm only. **Conventional columns with internal diameters of 1.0mm are available with industry standard CPI female fittings only. 1.0mm ID columns are not available in 300mm length.

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Dead Volume

Part Number:

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Compression Screw

5360

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Insert

355

Standard HPLC Column Formats – Rocket™ Rocket™ Columns • Speed, Resolution, and Symmetrical Peaks on Any HPLC System! • 33mm and 53mm Column Lengths Reduce Analysis Times 70-80% • 3µm and 1.5µm Porous Media Generate High Efficiency for Maximum Resolution • 7mm ID Maintains Optimum Peak Shape on Conventional HPLC Systems

1.5 or 3µm High Stainless Stainless AxialEfficiency Steel Compression Stainless Porous Packing Steel Column Steel Frit Tube Endfitting Piston Material

7mm

™

Rocket Columns deliver the speed and performance today’s applications demand, without the drawbacks that plague other high-speed HPLC techniques. Methods run three to five times faster on Rocket™ Columns than on conventional columns, with equal or better resolution. Packed with 3µm or 1.5µm materials, Rocket™ Columns generate high efficiency and excellent peak shape with acidic, basic or neutral compounds. Rocket™ columns work well on any HPLC system. Unlike conventional 4.6mm ID high-speed columns, Rocket™ Columns (Figure 1) don’t require special tubing, injectors or detectors for proper performance. Let Rocket™ Columns accelerate your methods today!

Rocket™ Columns’ Advantage A common approach to decrease HPLC analysis time is to use a shorter column, often packed with smaller particle packing material. The increased efficiency of the smaller particle packing tends to offset the shortened length. However, there are some potential drawbacks to this approach that you should consider. 1. Short, small particle columns impose tighter restrictions on the amount of extra-column dead volume (and delay volume) tolerated. They may not provide adequate separations and analysis times with the instrumentation you have available. 2. You may not be able to load as much material or as large an injection volume on the smaller particle sizes and smaller columns.

To minimize these drawbacks, Alltech designed a wider diameter short column for use with small particle packings. The Rocket™ diameter is 7mm instead of the traditional 4.6mm. This difference translates to a 2.3–fold increase in column volume for a given column length. Rocket™ Columns are used on conventional HPLC systems with little or no modification to the system. The 33mm and 53mm x 7mm columns packed with small particles generate sharp, symmetrical peaks on any system, even with significantly increased flow rates. Both mass and injection volume load are greater on the wider diameter columns. Higher resolution of early eluting peaks permits higher flow rates and more rapid analyses.

Rocket™ Columns’ Success Formula: Efficiency x Selectivity x Capacity = Resolution All successful separations require efficiency, selectivity and capacity. Rocket™ Columns optimize all three in a package that delivers unprecedented resolution and speed.

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3. Resolution of the early eluting peaks may be seriously reduced, since they will be the peaks most affected by extra-column band broadening.

356

Efficiency Rocket™ Columns are packed with 3µm or 1.5µm spherical particles that produce 120,000 and 200,000 plates/meter, respectively. These high efficiencies let 33mm and 53mm Rocket™ Columns deliver the resolution of much longer columns in a fraction of the run time. © Copyright 2003 Alltech Associates, Inc.

33mm or 53mm Bed Length

3860

Figure 1

Selectivity Rocket™ Columns are available packed with many popular, high-quality 3µm materials for quick, convenient acceleration of existing methods. Simply use a Rocket™ Column packed with the 3µm version of your method’s packing and watch run times shrink dramatically! If we don't offer your packing material of choice in a Rocket™ format, use the custom ordering instructions below.

Capacity Rocket™ Columns accept high sample loads for easy detection of impurities and minor components in the presence of major peaks. 1.5µm Rocket™ Columns produce 200,000 plates/meter efficiencies even at high sample loading levels. Rocket™ Columns use fully porous packing materials that maintain capacity and efficiency as sample mass increases, conditions where non-porous silica (NPS) columns fail. 33 x 7mm Rocket™ Columns packed with 3µm materials produce the fastest analysis times. When maximum resolution is required for complex samples, use 53 x 7mm or 33 x 7mm Rocket™ Columns packed with 1.5µm Platinum™ media (see p387 for Platinum™) or 53 x 7mm Rocket™ Columns packed with the 3µm packing of your choice. Find Rocket™ Columns listed by phase in pages 362-403. Rocket™ Analytical Column Specifications Material: Max. Temperature: Max. Pressure: Thread Type: Typical Use:

316 SS, PEEK 120°C 10,000psig 10-32 UNF (Industry Standard Ports) High Speed Analytical HPLC Applications

Custom Rocket™ Column Ordering Instructions Rocket™ Columns can be custom manufactured with most packing materials that have a particle size of 3µm or smaller.

Rocket™ Custom Column Options Part Number: C-6000R for Rocket™ Column Internal Diameter: 7mm Length: 33-, or 53mm Packing Material: Name and Particle Size (≤3µm)

Rocket™ Custom Column Prices COLUMN LENGTH

MEDIA PRICE CODE

3-7

8

33mm 53mm Contact your Alltech office or distributor for current or local prices.

Standard HPLC Column Formats – Rocket™ Triazine Herbicides

1 2 4 35

Universal Combinatorial High Speed Gradient Application

™ 1 Rocket Columns Shorten Run Time While Maintaining Resolution

6

6

2 4

7

0

3

5

8669

8957

5

10

15

4

3

1. Simazine 4. Atrazine 2. Simetryn 5. Ametryn 3. Prometon 6. Prometryn 7. Terbutryn 7

1. 2. 3. 4. 5.

5 2

1

Theophylline Acetylsalicylic Acid Propanolol Nortriptyline Trimipramine

20 Min.

Rocket™ Conventional HPLC Column

0

Conventional Column: Platinum™ EPS 100Å C18, 5µm, 150 x 4.6mm Rocket™ Column: Platinum™ EPS 100Å C18, 1.5µm, 33 x 7mm Mobile Phase: 0.025M Potassium Phosphate, Monobasic pH3:Acetonitrile (65:35) Flowrate: 1.0mL/min Detector: UV at 254nm

1

2

3

4

5

6 Min.

Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile Gradient: Time: 0 6 %B: 10 90 Flowrate: 2.5mL/min Detector: Alltech ELSD Drift Tube Temp: 45°C Nitrogen Flowrate: 1.75SLPM

Taxol and Related Taxanes in Yew (taxus brevifolia) 11

12

1. 10-Deactylbaccatin III 2. Baccatin III 3. 7-Xylosyl-10-deacetyltaxol B 4. Taxinine M 5. 7-Xylosyl-10-deacetyltaxol 6. 7-Xylosyl-10-deacetyltaxol C 7. 10-Deacetyltaxol

6

Column: Alltima™ CN, 3µm, 53 x 7mm Rocket™ Mobile Phase: A: 1-Methoxy-2-propanol B: Acetonitrile C: Water Gradient: Time: 0 8 %A: 3 3 %B: 20 65 %C: 77 32 Flowrate: 2.5mL/min Detector: UV at 227nm

10

5 1

13

78 9 2 4

6

9241

8. 7-Xylosyltaxol 9. Cephalomannine (Taxol B) 10. 7-Epi-10-deacetyltaxol 11. Taxol 12. Taxol C 13. 7-Epitaxol

3

hplc hplc

Xanthines

1

0

5

Rocket

10

15 Min.

Mobile Phase Consumption by 50%

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Reduce Run Time by 90%

0

1

2 Min.

Rocket™ Column Column: Mobile Phase: Flowrate: Detector:

Platinum™ EPS C18, 3µm, 33 x 7mm 0.010M Sodium Acetate, pH4.0:Methanol (70:30) 5.0mL/min UV at 254nm

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Platinum™ EPS C18, 5µm, 150 x 4.6mm 0.010M Sodium Acetate, pH4.0:Methanol (70:30) 1.0mL/min UV at 254nm

5

4

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3

Conventional Column: Mobile Phase: Flowrate: Detector:

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2

5

4

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3

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2

8679

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2 34 5

1

1. Xanthine 2. Theobromine 3. Theophylline 4. Caffeine 5. 8-Chlorotheophylline

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8728

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1

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7 Min.

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3

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1

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0

357

Standard HPLC Column Formats – LC/MS (Expedite) Expedite™ Columns

Alltima C18, 3µm, 10 x 2.1mm, 1.0mL/min

• Fast Gradients for High Throughput Screening (HTS) Applications • Column Design Preserves Packing Material Efficiencies and Peak Shapes • Available in 2 Diameters and 2 Lengths, with 10 Different Chemistries Expedite™ columns produce the high efficiency separations required by the fast gradient methods employed by high throughput screening labs. They are packed with 3µm spherical particles in 10mm and 20mm lengths in both 2.1mm and 4.6mm internal diameters. With small-volume columns, the design of the column hardware is critical to the performance of the column. The attributes of the high performance packing material can easily be lost when packed in poorly designed column hardware. The Expedite™ Columns produce narrow, symmetrical peaks under demanding gradient conditions (Figure 1).

5

4

1

9471

7

8

6

2

9

3

0

1

2

3 Min.

Alltima C18, 3µm, 20 x 2.1mm, 1.2mL/min 4 5

1

9462

7

3

8

6

9 Expedite™ hardware is available in 10 and 20mm lengths, and 2.1 and 4.6mm ID

2

Actual Size

0

1

3 Min.

2

Alltima C18, 3µm, 10 x 4.6mm, 2.0mL/min

20mm

1

9468

4 5 7 10mm

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Expedite your LC/MS analyses with Alltech’s high-speed, lowvolume Expedite™ hardware. These fast analysis columns packed with high resolution Alltech media increase laboratory efficency by doubling or even tripling sample throughput.

9

3

2 0

1

2

3 Min.

Alltima C18, 3µm, 20 x 4.6mm, 2.0mL/min 9467

1

4 5

Expedite™ Analytical Column Specifications Material: Max. Temperature: Max. Pressure: Thread Type: Typical Use:

316 SS, PEEK 120ºC 10,000 psig 10-32 UNF (Industry Standard Ports) High Speed Gradient HPLC Applications

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8

6

7 6 2

9

3

1

2

3 Min.

Alltech Expedite HPLC Columns PACKING MATERIAL

Alltima Platinum Prevail

PHASES

PARTICLE SIZE

C18, Phenyl, Silica EPS C18, Phenyl, Silica C18, Amide, Phenyl, Silica

3µm 3µm 3µm

PAGE NO.

370 Call* 393, 399

*Please contact technical support for packing materials not listed in this catalog.

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358

© Copyright 2003 Alltech Associates, Inc.

Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in CH3CN Gradient: Time: 0 2 %B: 5 100 Detector: UV at 254nm Figure 1 - shows 4 chromatograms of a test mixture on 2.1mm x 10mm and 20mm columns and on 4.6mm x 10mm and 20mm columns. While normally used with mass spec detectors in HTS applications, the Expedite™ columns can also be used with UV detectors with small volume flow cells. Contact your Alltech office or distributor for current or local prices.

Custom HPLC Column Formats – Stainless Steel Preparative Alltech manufactures preparative columns packed with popular Alltech packing materials as standard part numbers. These prep columns may be found in this catalog as follows:

Stainless Steel Preparative Columns • 7-, 10-, and 22-mm Internal Diameters • Type 316 Stainless Steel Construction • Preparative Guard Columns with Matching Chemistry

Alltech Preparative HPLC Columns

Replacement Piston/Frit Assembly

Endfitting 5544

Figure 1

Alltech’s preparative columns use stainless steel endfittings and PEEK pistons with built-in stainless steel frits attached to either 3/8" OD x 7mm ID, 1/2" OD x 10mm ID, or 1" OD x 22mm ID threaded tubing (Figure 1). All columns have industry standard endfittings. Preparative columns provide higher volume and mass loading capacities than analytical columns. The loading capacity for a particular column is dependent on the sample and the separation conditions. You can estimate the loading capacity for a preparative column if you know the loading capacity for an analytical column filled with the same packing and operated under similar conditions. Estimate the loading capacity for the preparative column using Equation 1. Table 1 lists the approximate increase in loading capacity (compared to 4.6mm ID analytical columns of the same length) for 7-, 10-, and 22-mm ID prep columns.

Equation (1) LC Prep. = LC Anal. x (ID Prep./ID Anal.)2 x (L Prep/L Anal.) = Loading Capacity = Column Internal Diameter = Column Length

372 376 380 381 Call* Call* 395 568

Choose a Prep-Guard guard column to protect your expensive preparative column and extend its life. The 33 x 7mm columns are packed with a number of popular chemistries on 10µm particles. See page 464.

Replacement Endfittings DESCRIPTION

QTY

PART NO.

Endfittings for 7mm ID Column for 10mm ID Column for 22mm ID Column

Ea Ea Ea

96505 96515 96525

DESCRIPTION

QTY

PART NO.

Pistons for 7mm ID Column for 10mm ID Column for 22mm ID Column

Ea Ea Ea

96507 96517 96527

PRICE

Replacement Pistons PRICE

Custom Preparative Ordering Instructions

4

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MEDIA PRICE CODE

1 100-199mm 200-500mm

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*Assumes prep column and analytical column are the same length, filled with the same packing material and operated with the same mobile phase and linear solvent velocity.

Preparative Custom Column Prices COLUMN LENGTH

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2.3X 4.7X 23X

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LOADING CAPACITY

Part Number: C-6000F for Preparative Column Internal Diameter: 7-, 10-, and 22mm (Larger ID Columns Available, please call for quote) Length: 100-, 150-, 250-, 300-, 500mm Packing Material: Name and Particle Size (5µm or larger particle size only)

hplc hplc

Table 1 - Approximate Loading Capacity Increase Compared to 4.6mm ID Analytical Column* 7mm 10mm 22mm

PAGE NO.

5, 10µm 5µm 5, 10µm 5, 10µm 5µm 5µm 5µm 5, 10µm

Preparative Custom Column Options

Equation 1

INCREASE IN PREP COLUMN ID

PARTICLE SIZE

C18, Si C18, Si C18, Si C18, Si C18, Si C18, Si C18, Si C18, C4, Si

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*Please contact technical support for packing materials not listed in this catalog.

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LC ID L

PHASES

Alltima Apollo Econosil Econosphere Platinum Platinum EPS Prevail Prosphere

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Column Tube

PACKING MATERIAL

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Preparative Column Specifications

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© Copyright 2003 Alltech Associates, Inc.

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Material: 316 SS, PEEK Max. Temperature: 120°C Max. Pressure: 8,000psig Thread Type: 10-32 UNF (Industry Standard ports) Typical Use: Preparative HPLC Applications

359

Price Codes for Custom Packed HPLC Columns The Widest Assortment of Custom Packed Columns Available Anywhere!

Alltech Media Price Codes (Continued) PACKING

1. Find your desired material in the packing media listing and identify the price code. 2. Select column hardware format from pages 355-359. For Stainless Steel Analytical Columns, see page 355. For Rocket™ Columns, see page 356. For Preparative Columns, see page 359. 3. Provide all the information requested for the selected hardware configuration. 4. Determine price (based on column dimensions and packing material price code) using the tables listed for each hardware format.

Price Codes for Custom Packed HPLC Columns Alltech Media Price Codes PRICE CODES

Adsorbosil™ C18, C8, C2, NH2, CN, Si Adsorbosphere® C8, C18, CN, NH2, Si, TMS, Phenyl SAX, SCX HS C18 HS Si UHS C18 OPA HS, OPA HR Catecholamine Nucleotide/Nucleoside CN-AQ Adsorbosphere® XL C18, C8 Silica C18-B SAX, SCX C18 300, C8 300 HAAX Allsphere™ ODS-2, C8, CN, NH2, Silica ODS-1, Phenyl, C1 C6, SAX, SCX Alltima™ C18, C8, Silica C18-LL, C18-WP, C4-WP, Phenyl, NH2, CN AQ AlphaBond™ C18, Silica, C8, Phenyl, CN, NH2 Apollo C18, Phenyl, Silica

3µM

5 6 5

5µM

7µM

10µM

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4 6 4 6

6 6 6 5 4 5

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Carbohydrate Anomer NH2 Econosil™ C18, C8, NH2, CN, Si Econosphere™ C18, C8, NH2, Si CN Ion Chromatography Allsep/Allsep A-2 Anion/R Anion Exclusion Anion/S Novosep A-1 Transition Metal Surfactant/R Surfactant C18, Surfactant C8 Universal Cation Macrosphere™ GPC 60, GPC 100, GPC 150, GPC 300, GPC 500, GPC 1000 GPC 4000, 300 WAX, 300 WCX, 300 SAX, 300 SCX RP 300 C4, RP 300 C8, RP 300 C18 Mixed-Mode™ C8/Cation C18/Cation C8/Anion Platinum™ C18, EPS C18 8 C8, EPS C8, Phenyl, CN, NH2, SAX, Silica, 300 C18, 300 EPS C18, 300 C8, 300 EPS C8, 300 C4, 300 EPS C4, 300 Silica Prevail™ C18, C8, Phenyl, CN, Amino Silica, Amide Organic Acid ProSphere 300™ C18, C4, PAH

PRICE CODES 5µM 7µM

10µM

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PACKING

1.5µM 3µM

360

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Price Codes for Custom Packed HPLC Columns Price Codes for Custom Packed HPLC Columns Merck KGaA Media Price Codes

AKZO Nobel Media Price Codes PRICE CODES 5µM 10µM

PACKING

AKZO Nobel Packings Kromasil™ C18, C8, C4, NH2, Silica

5

4

GL Sciences Media Price Codes PRICE CODES 5µM

PACKING

GL Sciences Packings Inertsil® ODS-2, C8, Silica Phenyl

6 7

Macherey-Nagel Packings Nucleosil® C18 (100Å - 4000Å), C8 (100Å - 500Å) C4 (300Å - 4000Å) Phenyl (100Å - 300Å), Diol (100Å-4000Å) CN (100Å - 500Å) NH2 (100Å - 120Å) Nitrophenyl (100Å) N (CH3)2 (100Å) SA (100Å), SB (100Å) Silica (50Å thru 4000Å)

3µM

Merck KGaA Packings LiChrosorb® Si-60 Silica, Si-100 Silica, RP-8, RP-18, NH2, Diol RP-Select B, CN LiChrospher® Si-60 Silica, NH2, CN, Diol 100 Si-100 Silica, RP-8, RP-8 Endcapped, RP-18, RP-18 Endcapped Si-300 Silica, Si-1000 Silica, Diol 1000 RP-Select B

PRICE CODES 5µM 10µM

5

5

4

6

6

5 5

5 5 5

5

Waters Media Price Codes

Macherey-Nagel Media Price Codes PACKING

PACKING

3µM

PRICE CODES 5µM 7µM

10µM

PACKING

3µM

PRICE CODES 5µM 10µM

Waters Packings Waters Spherisorb® 5

4

3

3

4

4 3

4

4

4

4

Silica (80Å), ODS-2, Octyl (C8), CN, NH2 ODS-1, Phenyl SAX, SCX, Hexyl (C6)

7

6 6 6

5 5

Whatman Media Price Codes 4

4 PACKING

5 4

3

4 3

Whatman Packings PartiSil® SAX, SCX ODS-3, C8, PAC, Silica ODS, ODS-2

PRICE CODES 5µM 10µM

7 6 6

6 5 5

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Contact your Alltech office or distributor for current or local prices.

361

Alltech Adsorbosil™ Improved Separations with Alltech Adsorbosil™

Cortisone Acetate, Prednisone, Hydrocortisone, USP Method

1 • Different Selectivity than Spherical Packing Materials • Irregularly Shaped 5µm or 10µm Particles • High Surface Area Silica • Fully Endcapped

2

Adsorbosil™ Characteristics 450m2/g 60Å 15% 10% 5, or 10µm

0

5

10

7227

PARTICLE SIZE

LENGTH X ID

INDUSTRY STANDARD PART NO.

5µm 5µm 5µm 5µm 10µm 10µm

150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

298122 298120 298072 298012 298102 298042

298128 298132 298078 298018 298108 298048

5µm 5µm 5µm 5µm 10µm 10µm

150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

298123 298121 298073 298013 298103 298043

298139 298143 298079 298019 298109 298049

C2

5µm 5µm 10µm 10µm

150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

298074 298014 298104 298044

298080 298020 298110 298050

CN

5µm 5µm 10µm 10µm

150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

298075 298015 298105 298045

298081 298021 298111 298051

NH2

5µm 5µm 10µm 10µm

150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

298076 298016 298106 298046

298082 298022 298112 298052

Silica

5µm 5µm 10µm 10µm

150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

298077 298017 298107 298047

298083 298023 298113 298053

hplc hplc

C8

20 Min.

Acetaminophen, Aspirin and Caffeine, USP Method

Adsorbosil™ Columns

C18

15

Column: Adsorbosil™ Silica, 5µm, 250 x 4.6mm Mobile Phase: Butyl Chloride:Water-Saturated Butyl Chloride: Tetrahydrofuran:Methanol:Glacial Acetic Acid (95:95:14:7:6) Flowrate: 1.0mL/min Detector: UV at 254nm

1 2

PACKING

1. Methyl Paraben 2. Cortisone Acetate 3. Prednisone 4. Hydrocortisone

4

Adsorbosil™ packings offer different selectivity than spherical packing materials, often resulting in improved separation. This packing is based on an irregularly shaped silica with high surface area and bonded-phase load. The silica is acid washed to remove trace metals and is then bonded with C18, C8, C2, CN, or NH2. All bonded phases (other than NH2) are endcapped to remove unreacted silanol functions.

Surface Area: Pore Size: Carbon Load, C18: Carbon Load, C8: Particle Size:

3

7228

WATERS INSTRUMENT PART NO.

PRICE

1. Acetaminophen 2. Caffeine 3. Benzoic Acid 4. Salicylic Acid

3

4

0

3

6

Column: Mobile Phase: Flowrate: Detector:

9 Min.

Adsorbosil™ C18, 5µm, 100 x 4.6mm Water:Methanol:Glacial Acetic Acid (69:28:3) 1.5mL/min UV at 275nm

DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

All-Guard™ Cartridges (Requires Guard Holder) C18 5µm 7.5 x 3.2mm 3 C18 5µm 7.5 x 4.6mm 3 C8 5µm 7.5 x 3.2mm 3 C8 5µm 7.5 x 4.6mm 3 C2 5µm 7.5 x 4.6mm 3 CN 5µm 7.5 x 4.6mm 3 NH2 5µm 7.5 x 4.6mm 3 Silica 5µm 7.5 x 4.6mm 3

PART NO.

PRICE

96353 96035 96354 96036 96037 96038 96039 96040

Guard Holders (Includes Direct-Connect Column Coupler) All-Guard™ Cartridge Holder Ea 80101

i

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

i

For complete details on Alltech All-Guard Columns, see pages 460-464.

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Adsorbosil™ Guard Columns

362

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech Adsorbosphere®, Adsorbosphere® HS & UHS Adsorbosphere® Columns

Adsorbosphere® • High Temperature Bonding for Exceptional Surface Coverage, Stability, and Reproducibility • Spherical, Fully Endcapped Select Adsorbosphere® Columns for applications requiring exceptional surface coverage and stability. Our high temperature bonding converts 25% to 40% more of the silanols to primary bonded phase than with standard, lower-temperature bonding. Endcapping and monomeric bonding reduce batch to batch variability. The ratio of primary to endcapped silanols is greater than with other materials for a more stable bonded phase. As endcapping is lost due to hydrolysis, there is less change in selectivity because endcapped sites are a smaller percentage of the total bonded phase. The Alltech Adsorbosphere® CN-AQ Column is a cyanopropyl phase designed for the separation of tricyclic antidepressants (TCA’s). Adsorbosphere® Specifications 200m2/gram 80Å 12% 3, 5, and 10µm

Surface Area: Pore Size: Carbon Load (C18): Particle Size:

Adsorbosphere® Ion Exchange Capacities PHASE

EXCHANGE CAPACITY (MEQ/GGRAM)

SAX, 5µm, 80Å SCX, 5µm, 80Å

0.21 0.72

Adsorbosphere® HS • High Carbon Load, Spherical C18 Packing Resists Aggressive Mobile Phases • High Surface Area for High Carbon Load Adsorbosphere® HS resists aggressive mobile phases. Highdensity bonding onto a high surface area spherical silica produces a C18 carbon load of 20%.

LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS FITTINGS PART NO.

3µm 5µm 5µm 3µm 3µm 5µm 5µm 10µm C8 (8% C) 5µm 5µm 10µm Phenyl 5µm 5µm CN 3µm 5µm 10µm CN-AQ 5µm 5µm NH2 3µm 5µm PAC (CN/NH2) 5µm Silica 5µm 5µm SAX 5µm SCX 5µm

100 x 4.6mm 250 x 1.0mm 250 x 2.1mm 33 x 7mm 53 x 7mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm 150 x 2.1mm 250 x 4.6mm 250 x 4.6mm 250 x 4.6mm

287092 97402 9945 50623 50625 287072 287062 287052 287122 287112 287102 287532 287542 287262 287202 287182 50005 500050 287282 287242 287302 9964 287012 287512 287472

287093 – – – – 287073 287063 287053 287123 287113 287103 287533 287543 287263 287203 287183 50035 500060 287283 287243 287303 – 287013 287513 287473

Adsorbosphere HS C18 (20% C) 3µm 3µm 5µm 5µm 7µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm

28780 28786 28817 28821 28844

28781 28787 28818 28822 28771

PACKING

PARTICLE SIZE

Adsorbosphere C18 (12% C)

PRICE

Adsorbosphere UHS C18 (30% C) 5µm 150 x 4.6mm 288118 288119 10µm 150 x 4.6mm 288128 288129 10µm 250 x 4.6mm 288133 288134

This hydrophobic bonded phase protects the silica from aggressive mobile phases like ion-pair reagents and buffers for long column life, and it is highly selective for organic compounds.

Adsorbosphere® All-Guard™ Guard Columns

Contact your Alltech office or distributor for current or local prices.

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82181

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96079

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96041 96042 96045 96047 96048 96044 96046 96049 96075

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PRICE

Guard Holders (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder 80101 © Copyright 2003 Alltech Associates, Inc.

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500m2/gram 60Å 30% 5 and 10µm

PART NO.

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UHS

350m2/gram 60Å 20% 3, 5, and 7µm

QTY

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HS

LENGTH X ID

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Adsorbosphere® HS & UHS Specifications

PARTICLE SIZE

All-Guard™ Cartridges (Requires Guard Holder) Adsorbosphere® C18 5µm 7.5 x 4.6mm 3 C8 5µm 7.5 x 4.6mm 3 Phenyl 5µm 7.5 x 4.6mm 3 CN 5µm 7.5 x 4.6mm 3 CN-AQ 5µm 7.5 x 4.6mm 3 NH2 5µm 7.5 x 4.6mm 3 Silica 5µm 7.5 x 4.6mm 3 SAX 5µm 7.5 x 4.6mm 3 SCX 5µm 7.5 x 4.6mm 3 Adsorbosphere® HS C18 5µm 7.5 x 4.6mm 3 Adsorbosphere® UHS C18 5µm 7.5 x 4.6mm 3

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Adsorbosphere® UHS C18 is Alltech’s ultra-high surface area (30% carbon load) support designed for high resolution chromatography of those species having similar structures. Examples include polynuclear aromatic hydrocarbons (EPA Method 610), benzidines (EPA Method 605), and closely related compounds including Vitamins D2 and D3, PTH amino acids, and various chemical isomers.

DESCRIPTION

○

• Ultra-High Surface Area Results in Exceptional Bonded Phase Coverage • Ideal for High Resolution Chromatography

Surface Area: Pore Size: Carbon Load (C18): Particle Size:

hplc hplc

Adsorbosphere® UHS

363

Alltech Adsorbosphere® XL Adsorbosphere® XL Specifications

Adsorbosphere® XL • A Variety of Surface Chemistries, Particle and Pore Sizes • C18-B Phase for Strongly Basic Analytes • SCX, SAX, HAAX Phases for Ion Exchange Separations

Surface Area: Pore Volume: Carbon Load: C18 C18-B C8 Particle Shape: Endcapped:

Adsorbosphere® XL phases are available in 90Å and 300Å pore sizes, for separations ranging from small molecules to large proteins. The Adsorbosphere® XL C18-B (base-deactivated) phase was developed specifically for the separation of strongly basic analytes. Mobile phase modifiers like TEA are typically not needed when using this phase in order to obtain symmetrical peaks.

90Å PACKINGS

300Å PACKINGS

200m2/g 0.51 cc/g

100m2/g 0.78cc/g

11% 12% 6% Spherical Yes

6% 3% Spherical Yes

Triton X 100

For ion exchange separations, Adsorbosphere® XL is available in strong cation exchange (SCX), strong anion exchange (SAX), and high affinity anion exchange (HAAX). The HAAX, 300Å phase combines negligible hydrophobic binding together with a high protein affinity. This allows proteins to bind from high ionic strength matrices that would normally require desalting.

8735

Adsorbosphere® XL Columns

hplc hplc

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

20237 20381 20390 20552 20675 20474

20286 20394 20462 20579 20546 20241

90Å 90Å

5µm 150 x 4.6mm 5µm 250 x 4.6mm

20548 20247

20344 20627

90Å 90Å 90Å 90Å 300Å 300Å

3µm 3µm 5µm 5µm 5µm 5µm

20598 20320 20676 20383 20356 20635

20602 20203 20607 20460 20391 20646

90Å 90Å 90Å 90Å 300Å 300Å

3µm 3µm 5µm 5µm 5µm 5µm

C18-B

C8

C18

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WATERS INSTRUMENT PART NO.

PARTICLE SIZE

PACKING

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

TMS (C1) 90Å 90Å

5µm 150 x 4.6mm 5µm 250 x 4.6mm

20386 20628

20452 20636

Silica

90Å 90Å

5µm 150 x 4.6mm 5µm 250 x 4.6mm

20365 20256

20398 20654

SCX

90Å 5µm 150 x 4.6mm 90Å 5µm 250 x 4.6mm 90Å 10µm 250 x 4.6mm

20525 20545 20719

20531 20564 20684

SAX

90Å 5µm 150 x 4.6mm 90Å 5µm 250 x 4.6mm 90Å 10µm 250 x 4.6mm

20538 20550 20314

20318 20561 20303

HAAX

300Å 3µm 150 x 4.6mm 300Å 7µm 150 x 4.6mm 300Å 7µm 250 x 4.6mm

20763 20774 20773

20765 20776 20775

PRICE

0

10

15 Min.

Column: Adsorbosphere® XL TMS (C1), 5µm, 250 x 4.6mm Mobile Phase: A: Water B: Methanol Gradient: Time: 0 15 %B: 60 70 Flowrate: 1.0mL/min Detector: UV at 254nm Ion Exchange Capacities PHASE

EXCHANGE CAPACITY (MEQ/GRAM)

SCX, 10µm, 90Å SAX, 10µm, 90Å HAAX, 7µm, 300Å

0.80 0.24 0.29

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

All-Guard™ Cartridges (Requires Guard Holder) C1 5µm 7.5 x 4.6mm 3 96093 C8 5µm 7.5 x 4.6mm 3 96090 C18 5µm 7.5 x 4.6mm 3 96088 C18-B 5µm 7.5 x 4.6mm 3 96089 Silica 5µm 7.5 x 4.6mm 3 96097 SCX 5µm 7.5 x 4.6mm 3 96099 SAX 5µm 7.5 x 4.6mm 3 96098 HAAX 7µm 7.5 x 4.6mm 3 96400

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DESCRIPTION

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5

Adsorbosphere® XL Guard Columns

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LENGTH X ID

INDUSTRY STANDARD PART NO.

PORE SIZE

PACKING

C18

PARTICLE SIZE

LENGTH X ID

PART NO.

3µm 3µm

33 x 7mm 53 x 7mm

50633 50635

PRICE

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Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

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Guard Holders (Includes Direct-Connect Column Coupler) Ea 80101 All-Guard™ Cartridge Holder

Adsorbosphere® XL Rocket™ Columns

364

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech Adsorbosphere® Specialty Columns Adsorbosphere® Catecholamine

Adsorbosphere® OPA

The Adsorbosphere® Catecholamine Column is a universal, reliable, and inexpensive means for routine analysis of indoles and catecholamines. Catecholamines from brain tissue, urine, etc., are easily resolved under isocratic conditions at room temperature. Plasma separations are possible using high quality ECD detectors and pumps designed specifically for minimal pressure pulsation. In all cases, a simple monochloroacetic acid mobile phase containing varying amounts of sodium octyl sulfate and disodium EDTA is used to control the elution profile.

Adsorbosphere® Catecholamine Columns

100 x 4.6mm 28916

28918

PRICE

Catecholamine 3µm

Adsorbosphere® Catecholamine Guard Columns DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

All-Guard™ Cartridges (Requires Guard Holder) Catecholamine C18 3µm 7.5 x 4.6mm 3 96076 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard™ Cartridge Holder Ea 80101

The OPA-HS column is 100 x 4.6mm and is best suited for the study of protein acid hydrolysates. The mobile phase system is very simple and the parameters can be changed to customize the elution profile. An extensive operating manual with a bibliography is included with the column; it describes the effects of pH, % organic modifier, and ionic strength on retention time. Graphs of these variables versus the retention time for the individual amino acids are provided to make optimization of the separation quick and uncomplicated. The OPA-HR column was developed for applications which require more resolution than simple hydrolysates. The 150 x 4.6mm column provides enhanced resolution of the amino acids through additional steps in the bonding protocol for the OPA-HR material. The OPA-HR column will give baseline separation of most amino acids found in physiological fluids. Each column is supplied with a batch chromatogram of an amino acid separation along with a quality control chromatogram for each individual column.

Adsorbosphere® Nucleotide-Nucleoside

Adsorbosphere® OPA Columns

®

The Adsorbosphere Nucleotide-Nucleoside column is a single reversed-phase column designed for the determination of both nucleotides and nucleosides. The column separates seven common nucleosides or twelve common mono-, di-, and triphosphate nucleotides using a buffer/methanol/tetrabutylammonium phosphate gradient.

Adsorbosphere® Nucleotide-Nucleoside Columns

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

28066

150 x 4.6mm

28072

28074

PRICE

Derivatization Reagents

WATERS

DESCRIPTION

PRICE

QTY

PART NO.

5g

35606

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

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For complete details on Alltech All-Guard Columns, see pages 460-464.

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PRICE

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All-Guard™ Cartridges (Requires Guard Holder) Nucleotide-Nucleoside C18 7µm 7.5 x 4.6mm 3 96077

OPA Crystals

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PRICE

Adsorbosphere® Nucleotide-Nucleoside Guard Columns DESCRIPTION

5µm

28064

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35624

LENGTH X ID

OPA-HR

LENGTH X ID

100 x 4.6mm

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Nucleotide-Nucleoside 7µm 250 x 4.6mm 35623

PACKING

5µm

WATERS INSTRUMENT PART NO.

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INSTRUMENT PART NO.

PARTICLE SIZE

OPA-HS

PACKING

INDUSTRY STANDARD PART NO.

hplc hplc

INDUSTRY STANDARD PART NO.

PARTICLE SIZE

○

INSTRUMENT PART NO.

LENGTH X ID

WATERS

Alltech developed two different reversed-phase columns specifically for the study of amino acids. The OPA-HS column is for high speed separations and the OPA-HR column is for the analysis of physiological samples where a higher degree of resolution is required.

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PARTICLE SIZE

PACKING

INDUSTRY STANDARD PART NO.

• Separates o-Phthalaldehyde Derivatives of Amino Acids • High Speed Column for Protein Hydrolysates • High Resolution Column for Physiological Samples • Complete Methodology Supplied with Each Column

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Guard Holders (Includes Direct-Connect Column Coupler) All-Guard™ Cartridge Holder Ea 80101

365

Alltech Allsphere™ Allsphere™ Analytical Columns

A Waters Spherisorb® Alternative

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

• Consider Allsphere™ for Your Waters Spherisorb® Applications • Priced Significantly Lower than Waters Spherisorb® Columns from Alltech

PACKING

ODS-1

We are confident that an Allsphere™ column will meet or exceed any specifications set for your Waters Spherisorb® applications and are prepared to back this up. We guarantee that if you purchase an Allsphere™ column and it fails to meet your Waters Spherisorb® application requirements, we will gladly take it back and credit your account for the full purchase price*.

80Å 5µm 150 x 4.6mm 778441 778443 80Å 5µm 250 x 4.6mm 778364 778357 80Å 10µm 250 x 4.6mm 778449 778455

ODS-2

80Å 80Å 80Å 80Å 80Å

3µm 3µm 5µm 5µm 10µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm

778487 778558 778545 778736 778553

778489 778560 778547 778738 778155

Octyl

80Å 80Å 80Å 80Å

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

778561 778609 778571 778744

778563 778611 778573 778746

Hexyl

80Å 5µm 150 x 4.6mm 778211 778217 80Å 5µm 250 x 4.6mm 778223 778224

Phenyl

80Å 5µm 150 x 4.6mm 778689 778691 80Å 5µm 250 x 4.6mm 778752 778754 80Å 10µm 250 x 4.6mm 778697 778699

Methyl

80Å 5µm 150 x 4.6mm 778238 778239 80Å 5µm 250 x 4.6mm 778244 778248

Cyano

80Å 5µm 150 x 4.6mm 778713 778715 80Å 5µm 250 x 4.6mm 778361 778362

Amino

80Å 5µm 150 x 4.6mm 778739 778741 80Å 5µm 250 x 4.6mm 778371 778372

Silica

80Å 80Å 80Å 80Å

SAX

80Å 5µm 250 x 4.6mm 778765 778767

SCX

80Å 5µm 250 x 4.6mm 778188 778189

Allsphere™ columns are available packed with 80Å spherical media in 3, 5 and 10µm particle size. A wide selection of surface chemistries are available to meet a variety of separation needs. *Offer limited to 90 days from date of purchase.

PORE PARTICLE SIZE SIZE

LENGTH X ID

PRICE

Alkyl Benzoic Acids

1

8321

2

1. 4-Butylbenzoic Acid 2. 3,5,-di-tert-Butylbenzoic Acid 3. 4-Hexylbenzoic Acid 4. 4-Octylbenzoic Acid 4

3

0

2

4

Column: Mobile Phase: Flowrate: Detector:

6

8 Min.

Allsphere C8, 5µm, 150 x 4.6mm Acetonitrile:Water:Acetic Acid (65:34:1) 1.0mL/min UV at 254nm

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

778382 778554 778389 778376

778383 778556 778391 778377

WATERS

Base Material: Particle Size: Pore Size: Surface Area:

PACKING ALLTECH

®

™

SPHERISORB

ALLSPHERE

Silica 3, 5, 10µm 80Å 220m2/g

Silica 3, 5, 10µm 80Å 220m2/g

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Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

i

For complete details on Alltech All-Guard Columns, see pages 460-464.

ODS-2

PORE SIZE PARTICLE SIZE

100Å 100Å

3µm 3µm

LENGTH X ID

PART NO.

33 x 7mm 53 x 7mm

50613 50615

PRICE

Allsphere™ Guard Columns DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

All-Guard™ Cartridges (Requires Guard Holder) Silica 5µm 7.5 x 4.6mm 3 96401 ODS-1 5µm 7.5 x 4.6mm 3 96402 ODS-2 5µm 7.5 x 4.6mm 3 96403 Octyl 5µm 7.5 x 4.6mm 3 96404 Hexyl 5µm 7.5 x 4.6mm 3 96405 Phenyl 5µm 7.5 x 4.6mm 3 96406 Methyl 5µm 7.5 x 4.6mm 3 96407 Cyano 5µm 7.5 x 4.6mm 3 96408 Amino 5µm 7.5 x 4.6mm 3 96409 SAX 5µm 7.5 x 4.6mm 3 96410 SCX 5µm 7.5 x 4.6mm 3 96411

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hplc hplc

Allsphere™ Rocket™ Columns* Physical Characteristics Comparison

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Guard Holders (Includes Direct-Connect Column Coupler) All-Guard™ Cartridge Holder Ea 80101

366

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech Alltima™ Analytical Columns Alltima™ HPLC Columns

Basic Drugs

2

• Analyze Acids, Bases, and Neutrals in One Run • Symmetrical Peaks without Mobile Phase Modifiers • Stable Bonding for Long Column Lifetimes • LC/MS and Microbore, Analytical, and Preparative Formats • 3µm, 5µm and 10µm Particle Sizes Alltima™ HPLC columns offer the ultimate in chromatography, whether you are analyzing acids, bases, or neutrals (Figure 1). Alltima™ phases are base-deactivated to eliminate activated silanols, the primary source of tailing on silica-based phases. Applications for strongly-basic compounds developed on Alltima™ phases generally do not require mobile phase modifiers like triethylamine to produce sharp, symmetrical peaks (Figure 2). Alltima™ phases are polymerically bonded and double endcapped to provide long column lifetimes, even when using harsh mobile phases. As demonstrated in Figure 3, column capacity and selectivity remained constant after washing the column for 500 hours with an aggressive acetonitrile/ trifluoroacetic acid mobile phase at elevated temperature. Alltima™ C18-WP (wide-pore) and C4-WP phases were developed for peptides and proteins using base-deactivated silica to ensure symmetrical peaks and high recoveries (chromatogram 9113 page 369).

8193

1 1. 2. 3. 4.

3 4

0

5

10

Column: Mobile Phase: Flowrate: Detector:

Procainamide Acetaminophen Theophylline Caffeine

15 Min. Alltima™ C8, 5µm, 150 x 4.6mm 20:80 Methanol: 50mM KH2PO4, pH3.0 1.0mL/min UV at 254nm

Figure 2 - Applications for strongly basic compounds developed on Alltima phases generally do not require mobile phase modifiers like triethylamine to produce sharp, symmetrical peaks.

Initial Separation

Alltima™ products are available in a variety of bonded phases, particle sizes, pore sizes and column formats, offering the versatility needed to overcome challenging separation problems.

2

1

7553

3 45

Alltima™ Packing Specifications

0

5 10 15 20 25 30 35 40 45 50 55 Min.

Chromatographic Conditions 1. Gly-Tyr 2. Val-Tyr-Val 3. Met-Enkephalin 4. Leu-Enkephalin 5. Angiotensin II

Alltima™ C18, 5µm, 150 x 4.6mm A: Water:Acetonitrile: TFA (95:5:0.1) B: Water:Acetonitrile: TFA (25:75:0.1) Time: 0 60 % B: 5 30 1mL/min UV at 220nm

Column: Mobile Phase:

Gradient: Flowrate: Detector:

Acid, Base, and Neutral Test Mix 5

3

After 500 Hours of Washing at 60° C 2 1

7554

3

45

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2

8589 1. Uracil 2. Pyridine 3. Phenol 4. N,N-Dimethylaniline 6 5. 4-Butylbenzoic Acid 6. Toluene

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4

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1

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Yes Yes Yes Yes Yes Yes Yes Yes No —

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ENDCAPPED

16% 15% 17% 9% 9% 6% 7.5% — — —

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CARBON LOAD

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PARTICLE SIZE

3, 5, 10µm 3, 5µm 5µm 5µm 3, 5, 10µm 5µm 3, 5µm 3, 5µm 3, 5µm 3, 5, 10µm

hplc hplc

C18 AQ C18-WP C18-LL C8 C4-WP Phenyl Cyano Amino Silica

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10 Min.

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8

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6

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4

0

5 10 15 20 25 30 35 40 45 50 55 Min.

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Contact your Alltech office or distributor for current or local prices.

Figure 3 - Column capacity and selectivity remained constant after washing an Alltima™ C18 column for 500 hours with 0.5% TFA:Acetonitrile(50:50) at 60° C. © Copyright 2003 Alltech Associates, Inc.

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Figure 1 - Separate acidic, basic, and neutral compounds in one run.

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Alltima™ C18, 5µm, 150 x 4.6mm 40:60 50mM KH2P04, pH3:Acetonitrile 1.0mL/min UV at 254nm

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2

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0

Column: Mobile Phase: Flowrate: Detector:

367

Alltech Alltima™ Analytical Columns Alltima™ Rocket™ Columns

Alltima™ Standard Analytical Columns LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

3µm 3µm 3µm 5µm 5µm 10µm 10µm

50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

81412 81382 81387 88052 88056 88302 88307

— 81383 81388 88053 88057 88303 88308

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

88935 88940 88925 88930

88955 88960 88945 88950

C18-WP

5µm 5µm

150 x 4.6mm 250 x 4.6mm

81194 81196

81195 81197

C18-LL

5µm 5µm

150 x 4.6mm 250 x 4.6mm

88069 88099

88082 88235

C8

3µm 3µm 3µm 5µm 5µm 10µm 10µm

50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

81413 81392 81397 88072 88076 88312 88317

— 81393 81398 88073 88077 88313 88318

C4-WP

5µm 5µm

150 x 4.6mm 250 x 4.6mm

81198 81202

81199 81204

Phenyl

3µm 3µm 3µm 5µm 5µm

50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

81178 81181 81188 88087 88092

— 81184 81191 88088 88093

Cyano

3µm 3µm 3µm 5µm 5µm

50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

81179 81182 81189 88180 88189

— 81186 81192 88182 88191

Amino

3µm 3µm 3µm 5µm 5µm

50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

81180 81183 81190 88205 88217

— 81187 81193 88207 88218

3µm 3µm 3µm 5µm 5µm 10µm 10µm

50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

81414 81404 81409 88123 88171 88322 88327

— 81405 81410 88124 88172 88323 88328

PACKING

C18

PRICE

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LENGTH X ID

PART NO.

C18

3µm 3µm

33 x 7mm 53 x 7mm

50603 50605

AQ

3µm

53 x 7mm

50765

C8

3µm 3µm

33 x 7mm 53 x 7mm

50607 50609

PRICE

Alltima™ Guard Columns DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

™

All-Guard Cartridges (Requires Guard Holder) C18 5µm 7.5 x 3.2mm 3 C18 5µm 7.5 x 4.6mm 3 AQ 5µm 7.5 x 4.6mm 3 C18-WP 5µm 7.5 x 4.6mm 3 C18-LL 5µm 7.5 x 4.6mm 3 C8 5µm 7.5 x 3.2mm 3 C8 5µm 7.5 x 4.6mm 3 C4-WP 5µm 7.5 x 4.6mm 3 Phenyl 5µm 7.5 x 4.6mm 3 Cyano 5µm 7.5 x 4.6mm 3 Amino 5µm 7.5 x 4.6mm 3 Silica 5µm 7.5 x 4.6mm 3

96361 96080 96344 96412 96285 96362 96081 96413 96082 96084 96085 96083

Guard Holders (Includes Direct-Connect Column Coupler) All-Guard™ Cartridge Holder Ea 80101

i

For complete details on Alltech All-Guard Columns, see pages 460-464.

Alltima™ C18 Validation Kit When validating a method, Alltima™ is the column of choice because of its excellent batch to batch, and column to column reproducibility. The Alltima™ C18 Method Validation Kit helps test method ruggedness prior to routine use. This Kit contains 3 columns made from 3 different batches of either 3µm or 5µm Alltima™ C18 packing.

Alltima™ C18 Validation Kit DESCRIPTION

PART NO.

PRICE

™

Alltima C18 Method Validation Kits 100 x 4.6mm, 3µm Kit (3 Columns from 3 Different Batches) 150 x 4.6mm, 5µm Kit (3 Columns from 3 Different Batches) 250 x 4.6mm, 5µm Kit (3 Columns from 3 Different Batches)

11350 11541 11543

i

Other dimensions and formats available. See page 370 for LC/MS formats, page 371 for Narrow Bore formats, and page 372 for Preparative formats. See pages 355-361 for custom HPLC columns.

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Silica

PARTICLE SIZE

PACKING

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hplc hplc

AQ

PARTICLE SIZE

368

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech Alltima™ Analytical Columns Antihistamines

Tricyclic Amines

4

1. 2. 3. 4. 5.

2 1

10

15

20

2

Tripelennamine Triprolidine Cyclizine Chlorcyclizine Meclizine

1. Fluphenazine HCL 2. Chlorpromazine HCL 3. Phenothiazine HCL

1

25 Min.

Column: Alltima™ C8, 5µm, 250 x 4.6mm Mobile Phase: A: 0.05M Potassium Phosphate, pH3.2 B: Acetonitrile Gradient: Time: 0 5 20 25 %B: 25 25 70 70 Flowrate: 1.3mL/min Detector: UV at 220nm

0

1

2

Column: Mobile Phase: Flowrate: Detector:

3

4

4

Flowrate: Detector:

8

7 8 Min.

Peptides

1

9113

1. Ethotoin 2. Phenobarbital 3. Methsuximide 4. Mephenytoin 5. Carbamazepine 6. Diazepam

123

Column: Mobile Phase: Gradient:

6

7233

6

0

5

Alltima™ CN, 3µm, 150 x 4.6mm Acetonitrile:10mM KH2PO4, pH2.6 (50:50) 1.0mL/min UV at 254nm

Anticonvulsants 5

4

12

16

1. Methionine 2. Physalaemin 3. Leucine Enkephalin 4. Substance P

4

2 3

0

20 Min.

1

2

3

5 Min.

4

Column: Alltima™ C18-WP, 5µm, 150 x 4.6mm Mobile Phase: A: 0.15% TFA in H2O B: 0.13% in 95:5 Acetonitrile:H2O Gradient: Time: 0 10 %B: 30 40 Flowrate: 1.0mL/min Detector: UV at 220nm

Alltima™ Phenyl, 5µm, 250 x 4.6mm A: Water B: Acetonitrile Time: 0 20 % B: 15 60 1.0mL/min UV at 254nm

hplc hplc

Dimethicone

Alkaloids in Goldenseal Root 2

1 8636

9124

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1. Hydrastine 2. Berberine

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5

9105

5

3

0

3

7232

Contact your Alltech office or distributor for current or local prices.

1

2 Min.

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Alltima™ C18, 3µm, 53 x 7mm Rocket™ Methanol:20mM KH2PO4, pH2.6 (48:52) 3.0mL/min UV at 235nm

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0 Column: Mobile Phase: Flowrate: Detector:

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Alltima C8, 5µm, 150 x 4.6mm A: Acetonitrile B: Chloroform Time: 0 20 % B: 25 75 1.0mL/min Alltech ELSD

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20 Min.

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Flowrate: Detector:

16

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Gradient:

12 ™

© Copyright 2003 Alltech Associates, Inc.

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Column: Mobile Phase:

8

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4

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0

369

Alltech Alltima™ LC/MS Columns Expedite™ hardware is available in 10 or 20mm lengths, and 2.1 or 4.6mm ID

Alltima™ Phases Are Perfectly Suited for LC/MS Applications • Stable in Popular LC/MS Mobile Phases • No Bonded Phase Bleed • Analyze Acids, Bases, and Neutrals with Simple, Volatile Mobile Phases • Available in New Expedite™ Hardware for Fast, Low-Volume Applications

Actual Size 20mm

Alltima™ LC/MS columns utilize advanced column technology to give you increased compatibility with your LC/MS detector. Alltima's highly stable bonded phases show no detectable bleed, giving you rock steady baselines and no retention time drift. The bonded phases are designed to be highly retentive allowing use of mobile phases with high organic content. This leads to better mobile phase compatibility and higher sensitivity on your MS detector. Alltima™ LC/MS columns are available in a wide variety of column dimensions and formats to cover every LC/MS need.

10mm

Expedite your LC/MS analyses with Alltech’s high-speed, lowvolume Expedite™ hardware. These fast analysis columns packed with high resolution Alltima™ media increase laboratory efficency by doubling or even tripling sample throughput.

Alltima™ LC/MS Columns PACKING

C18

™

Expedite Expedite™

™

Expedite Expedite™

Phenyl

Expedite™ Expedite™

LENGTH X ID

PART NO.

3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm

10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm 33 x 7mm

43836 43803 43849 43821 43852 43837 43851 43853 43864 50603

3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm

10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm

43809 43865 43835 43866 43813 43883 43822 43892 43854

3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm

10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm

43891 43801 43876 43823 43884 43889 43886 43824 43855

PRICE

1

4 5 7 6 2

0

Expedite™ Expedite™

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™

9

1

Column: Mobile Phase: Gradient: Flowrate: Detector:

2

9467 1. Uracil 2. Theophylline 3. Acetophenone 4. Propiophenone 5. Butyrophenone 6. Benzophenone 7. Valerophenone 8. Heptanophenone 9. Octanophenone

3 Min.

Alltima™ C18, 3um, 20 x 4.6 A: 0.1% TFA in H2O B: 0.08% TFA in H2O 5-100%B over 2 minutes hold 1 min 2.0mL/min UV at 254nm

Barbiturates 9433 5

1. Barbital 2. Phenobarbital 3. Talbital 4. Amobarbital 5. Mephobarbital

1 3 4

0

1

2 Min.

i

See page 358 for more information on Expedite™ Hardware.

Column: Mobile Phase: Flowrate: Detector:

Alltima™ C18, 3µm, 50 x 2.1mm 40:60 CH3CN:50mM Ammonium Acetate, pH5.4 0.5mL/min UV at 230nm

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Expedite Expedite™

8

3

2

Silica

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hplc hplc

Expedite™ Expedite™

Phenone Homologs

PARTICLE SIZE

370

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech Alltima™ Narrow-Bore Columns Alltima™ Narrow-Bore HPLC Columns

Narrow Bore Columns Increase Mass Sensitivity

• Increase Mass Sensitivity • Reduce Solvent Consumption • Available in 3.2mm, 2.1mm, and 1.0mm ID formats When compared with standard HPLC analytical (4.6mm ID) columns, narrow-bore columns offer increased mass sensitivity and reduced consumption of expensive solvents. Narrow-bore columns produce a larger peak response than standard 4.6mm ID columns due to the decreased diffusion the sample experiences as it passes through the narrower column. Narrow-bore columns are recommended for applications requiring detection of minor components such as degradants, as well as when sample size or concentration is low.

1

2

3

Standard Analytical Column 4.6mm ID 1.0mL/min

4

Solvent Minimizer 50 Column 3.2mm ID 0.5mL/min

Mass Sensitivity

Solvent Miser Column 2.1mm ID 0.2mL/min

Sample dilution within a column decreases as column inner diameter decreases. The same sample mass injected into two columns with differing inner diameters will elute from the narrower column at a higher concentration. This can result in a significant increase in detector response, as demonstrated in chromatogram 8137. When compared with 4.6mm ID columns, 2mm ID columns are five times more mass sensitive, while 1mm ID columns are twenty times more mass sensitive.

9 Min.

Alltima™ C18, 5µm, 150mm Acetonitrile:Water (58:42) 1µL

Table 1 - Solvent Consumption Data 4.6MM

3.2MM

2.1MM

1.0MM

1.0

0.50

0.2

0.05

100% 12.0 120.0 —

50% 5.76 57.6 52%

20% 2.4 24.0 80%

5% 0.6 6.0 95%

† To produce equivalent mobile phase linear velocity. * Based on 12 minutes per injection, 8 hours per day, 50 weeks per year. Results indicate the microbore column consumes the same amount of mobile phase in one year as does a 4.6mm ID column in 2.5 weeks.

LENGTH

PART

SOLVENT MISER 2.1MM ID NO. PRICE

PART

MICROBORE** 1.0MM ID NO. PRICE

88370 88371

88384 88385

Alltima™ C18-LL, 5µm 150 88386 250 88387

88388 88389

88390 88391

Alltima™ C8, 5µm 150 88392 250 88393

88372 88373

88394 88395

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Alltima™ C18, 5µm 150 88383 250 16921

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SOLVENT MINIMIZER 50 3.2MM ID PART NO. PRICE

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Alltima™ Narrow-Bore Columns

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Flowrate (mL/min.)† Relative Mobile Phase Consumption Solvent Usage/Inj. (mL) Annual Solvent Usage (L)* Percent Solvent Savings

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COLUMN INNER DIAMETER

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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**All Columns 1/8" OD with Industry Standard Fittings.

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Microbore 1.0mm ID Columns require more stringent controls on instrument dead volume. Connecting tubing should be kept extremely short, with an inner diameter between 0.004" and 0.007". Injection volumes are typically 0.1 to 1µL. Flowrates range from 10 to 200µL/min and require a pump capable of stable, pulse-free mobile phase delivery in this range. The detector flow cell volume should be no greater than 2 to 3µL.

Column: Mobile Phase: Injection Volume:

6

hplc hplc

Solvent Miser™ 2.1mm ID Columns work well on most HPLC equipment without major modifications. Injection volumes are typically 1 to 5µL at flowrates of 0.1 to 1mL/min. Equipment designed for use with standard analytical columns will provide good results as long as unnecessary dead volume (heat exchanger, long connecting tubes, large sample loops, etc.) is eliminated.

3

○

Solvent Minimizer 50™ 3.2mm ID Columns offer the advantage of a 50% reduction in mobile phase consumption and increased mass sensitivity without any equipment modification. HPLC equipment designed for use with standard 4.6mm ID analytical columns will produce excellent results.

0

○

Mobile phase consumption can be reduced considerably by switching from a standard 4.6mm ID column to a narrow-bore column. Mobile phase consumption is reduced because the flowrate required to maintain an equivalent mobile phase linear velocity decreases as the column inner diameter decreases (Table 1).

Microbore Column 1.0mm ID 0.05mL/min

○

Mobile Phase Consumption

8137

1. Uracil 2. Phenol 3. N,N-Diethyl-m-toluamide 4. Toluene

371

Alltech Alltima™ Preparative Columns Alltima Media Scales Up Easily Analytical Column 250 x 4.6mm

8585

Semi-Preparative Column 250 x 10mm 4 2

4 2

1

0

2

4

6

3

1. Uracil 2. Aniline 3. N,N-Diethyl-m-toluamide 4. N,N-Dimethylaniline

3

1

0

10 Min.

8

2

Alltima™ Prep Columns*

C18

hplc hplc

C8

INDUSTRY STANDARD PART NO.

5µm 5µm 5µm 5µm 5µm 5µm 10µm 10µm 10µm 10µm

150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm

81123 81129 81102 88063 81106 81105 81107 88334 81108 88335

5µm 5µm 5µm 5µm 5µm 5µm 10µm 10µm 10µm 10µm

150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm

81132 81133 81109 88081 81112 81110 81114 88336 81115 88337

5µm 5µm 5µm 5µm 5µm 5µm 10µm 10µm 10µm 10µm

150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm

81135 81136 81117 81116 81119 81118 81121 88338 81125 88339

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10 Min.

Alltech Media Scales Up Easily Catechins in Green Tea 10140

Preparative Column

1. Caffeine 2. Epicatechin 3. Epigallocatechin Gallate

3 1

0

2

4

2

6

Column: Mobile Phase: Flowrate: Detector:

8

10

12

14

16

18

Alltima™ C18, 5µm, 150 x 22mm (87:13) 0.05% TFA:Acetonitrile 23mL/min Alltech ELSD

Analytical Column 10139

1. Caffeine 2. Epicatechin 3. Epigallocatechin Gallate 3

1

*Larger prep columns available upon request.

2

Alltima™ Prep Guard Columns

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8

PRICE

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Silica

LENGTH X ID

6

Column: Alltima C18, 10µm, 250 x 10mm Mobile Phase:50mM KH2PO4, pH3.0:Acetonitrile (40:60) Flowrate: 4.73mL/min Detector: UV at 254nm

Column: Alltima C18, 10µm, 250 x 4.6mm Mobile Phase:50mM KH2PO4, pH3.0:Acetonitrile (40:60) Flowrate: 1.0mL/min Detector: UV at 254nm

PARTICLE SIZE

4 ™

™

PACKING

8586

372

DESCRIPTION

C18 Silica

i

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

10µm 10µm

33 x 7mm 33 x 7mm

1 1

970203 970204

See page 359 for more information on Preparative column hardware. © Copyright 2003 Alltech Associates, Inc.

PRICE

0

2

4

6

8 10 12 14 16 18 20 Min.

Column: Alltima™ C18, 5µm, 150 x 4.6mm Mobile Phase: (87:13) 0.05% TFA:Acetonitrile Flowrate: 1.0mL/min Detector: Alltech ELSD Contact your Alltech office or distributor for current or local prices.

Alltech Alltima™ Antibacterials

Natural Products 2

8764

3

8887

1 1. Oxytetracycline Dihydrate 2. Tetracycline HCL 3. Meclocycline Sulfosalicylate Salt

1 2

3

1. 2. 3. 4. 5. 6.

5 6 4

0

2

4

6

8

0

10 Min.

Flavonoids

Acids, Bases, and Neutrals 9271

1. 2. 3. 4.

2

15 Min.

Column: Alltima™ C18, 3µm, 100 x 4.6mm Mobile Phase: A: 50mM KH2PO4, pH 2.5 B: CH3CN Gradient: Time: 0 15 %B: 20 40 Flowrate: 1.0mL/min Detector: UV at 280nm

Column: Alltima™ C18, 3µm, 100 x 4.6mm Mobile Phase: A: 50mM KH2PO4, pH 3.0 B: CH3CN Gradient Time: 0 10 %B: 15 40 Flowrate: 1.0mL/min Detector: UV at 254nm

1

10

5

Naringin Neohesperidin Myricetin Quercetin Naringenin Kaempferol

8760

2

Myricetin Quercetin Apigenin Kaempferol

1. Uracil 2. Pyridine 3. Phenol 4. N,N-Dimethylaniline 5. 4-Butylbenzoic Acid 6. Toluene

4 5 1

4

6

3

3

0

Column: Mobile Phase: Flowrate: Detector:

1

3

4

5 Min.

Alltima™ C18, 3µm, 100 x 4.6mm 50mM KH2PO4, pH3.0:CH3CN (40:60) 1.0mL/min UV at 254nm

hplc hplc

Tween 80

2

Aromatic Acids

3

7809

7373

4

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 Min.

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5

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Alltima™ C18, 5µm, 150 x 4.6mm 50mM KH2PO4, pH 3.2:CH3OH (60:40) 1.0mL/min UV at 254nm © Copyright 2003 Alltech Associates, Inc.

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Column: Mobile Phase: Flowrate: Detector:

○

Column: Alltima™ C18, 5µm, 250 x 4.6mm Mobile Phase: H2O:CH3OH (40:60) Flowrate: 1.0mL/min Detector: Alltech ELSD Contact your Alltech office or distributor for current or local prices.

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0

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2

1. 4-Hydroxy-3methoxy-mandelic Acid 2. Homovanillic Acid 3. Vanillic Acid 4. Salicylic Acid 5. Benzoic Acid

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Alltima™ C18 Rocket™, 3µm, 53 x 7mm 0.1% TFA in H2O, pH 0.95:0.1% TFA in CH3CN (65:35) 2.5mL/min Alltech ELSD

1

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5 Min.

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4

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Column: Mobile Phase: Flowrate: Detector:

3

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2

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1

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0

373

Alltech Alltima™ Taxol® and Related Taxanes in Yew (taxus brevifolia)

Alprazolam and Triazolam by the USP XXII Method

7368

9221

1. 10-dDeacetylbaccatin III 2. Baccatin III 3. 7-Xylosyl-10-deacetyltaxol B 1 2 7 4,5 6 3

0

2

1

8 91011

3

4

13

12

5

6

7

™

Column: Mobile Phase: Flowrate: Detector:

4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

1. Triazolam 2. Alprazolam

Taxinine M 7-Xylosyl-10-deacetyltaxol 7-Xylosyl-10-deacetyltaxol C 10-Deacetyltaxol 7-Xylosyltaxol Cephalomannine (Taxol® B) 7-Epi-10-deacetyltaxol Taxol® Taxol® C 7-Epitaxol

1 2

0

8 Min. ™

Alltima Phenyl Rocket , 3µm, 53 x 7mm 0.05% TFA in H2O:CH3OH:CH3CN (50:19:31) 2.5mL/min UV at 230nm

2

4

Column: Mobile Phase: Flowrate: Detector:

Metal Chelators

1

6

8

10

12

14

16

0

2

Tricyclic Amines

1 2

7366

1. 2,2’-Dipyridyl 2. 8-Hydroxyquinoline

6 Min.

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4 3

7596

10

6

12 14 15

7

1. 2. 3. 4. 5. 6. 7.

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8

5

9

0

5

10

15

Asp Glu His Ser Arg Gly Thr

8. 9. 10. 11. 12. 13. 14. 15.

3

4

5

6

™

7 Min.

Alltima™ Cyano, 5µm, 250 x 4.6mm 50mM NaH2PO4, pH3.0: CH3CN (50:50) 1.5mL/min UV at 254nm

2

20 Min.

Column: Alltima C8, 5µm, 100 x 4.6mm Mobile Phase: A: 50mM Sodium Acetate, pH5.7:THF (95:5) B: CH3OH Gradient: Time: 0 15 %B: 10 65 Flowrate: 2.0mL/min Detector: Fluorescence Ex: 305-395nm Em: 430-470nm © Copyright 2003 Alltech Associates, Inc.

10124

3 6

Ala Tyr Met Val Phe Ileu Leu Lys

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2

Analgesics

11 13 2

374

1

Column: Mobile Phase: Flowrate: Detector:

OPA Derivatized Amino Acids 1

1. Fluphenazine HCl 2. Chlorpromazine HCl 3. Phenothiazine

3

0

Alltima™ C18, 5µm, 150 x 4.6mm 25mM K2HPO4, pH7.0:THF:CH3OH (50:25:25) 1.0mL/min UV at 254nm

hplc hplc

Column: Mobile Phase: Flowrate: Detector:

4

20 Min.

Alltima Silica, 5µm, 300 x 4.6mm CH3CN:CHCl3:i-BuOH:H2O:Glacial HOAc (85:8:5:2:0.05) 2.0mL/min UV at 254nm

8584

2

18

™

1

1. Aspirin 2. Acetaminophen 3. Naproxen 4. Fenprofen 5. Ibuprofen 6. Diclofenac

4 5

0

2

4

6

8 ™

10 Min.

Column: Alltima C8, 5µm, 150 x 4.6mm Mobile Phase: A: 50mM KH2PO4 pH3.3 B: Methanol C: Acetonitrile Gradient: Time: 0 2 5 %B: 10 10 30 %C: 10 10 25 Flowrate: 2.0mL/min Detector: UV at 280nm Contact your Alltech office or distributor for current or local prices.

Alltech Alltima™ MDA & MDMA Analogs 1

Triglycerides in Walnut Oil 9522

2

10014

2

1. 2,3-MDA 2. 3,4-MDA 3. 2,3-MDMA 4. 3,4-MDMA

3

1. Trilaurin (LaLaLa) 2. Trilinolein (LLL) 3. 1,2-Dilinoleyl-3-Oleyl-rac glycerol (LLO) 4. 1,2-Dilinoleyl-3-Palmitinrac-glycerol (LLP) 5. 1-Palmitin-2-Olein-3Linolein rac-glycerol (POL) 6. 1,2-Dipalmytyl-3-Linoleinroc glycerol (PPL)

3

4

4 56

1

0

1

2

Column: Mobile Phase: Flowrate: Detector:

3

0 2

4 Min.

Alltima™ Silica, 3µm, 100 x 4.6mm Acetonitrile:1.0% Ammonium Hydroxide (75:25) 2.0mL/min UV at 280nm

4

6

8 10 12 14 16 Min.

Column: Alltima™ C18, 3µm, 150 x 4.6mm Mobile Phase: A: Dichloromethane B: Acetonitrile Gradient: Time: 0 10 18 20 %B: 70 55 70 70 Flowrate: 1.5mL/min Detector: Alltech ELSD

Aspartame

Iridoid Compounds in Devil's Claw 9191

9032

1. Degradant 2. Aspartame

2

1. 8-para-Coumaroyl Harpagoside 2. Harpagoside 2

1 1

5

0

6 Min.

Alltima™ C18, 3µm, 150 x 4.6mm 50mM KH2PO4, pH 2.75:CH3OH (80:20) 1.0mL/min UV at 220nm

1

2

Column: Mobile Phase: Flowrate: Detector:

hplc hplc

1. p-Aminobenzoic Acid 2. Benzocaine 3. Tetracaine 4. Dibucaine 5. Butacaine

9210

1. Streptomycin 2. Amikacin 3. Tobramycin 4. Neomycin

3

1

4

2

4

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6

8

10

12 Min.

0

1

2

3

4

5

6

7

8

9 Min.

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© Copyright 2003 Alltech Associates, Inc.

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Column: Alltima™ C18, 5µm, 250 x 4.6mm Mobile Phase: 0.3% Pentafluropropionic Acid in CH3OH:0.3% Pentafluropropionic Acid in 3.7mM Ammonium Formate, pH2.7 Flowrate: 1.0mL/min Detector: Alltech ELSD

○

Column: Alltima™ C8, 5µm, 150 x 4.6mm Mobile Phase: A: Water B: Methanol C: Acetonitrile Gradient: Time: 0 7 %B: 15 45 %C: 15 35 Flowrate: 1.5mL/min Detector: UV at 230nm Contact your Alltech office or distributor for current or local prices.

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0

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1

6

2

10118

5

5

Aminoglycoside Antibiotics

4

2

4

Alltima™ C18, 3µm, 53 x 7mm Rocket Dioxane:CH3OH:0.05% TFA (2:23:75) 3.0mL/min UV at 285nm

Anesthetics

3

3

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3

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375

Alltech Apollo A Great Value - The Best Price/Performance Ratio Available Anywhere • High-Purity, Base-Deactivated Silica for Unsurpassed Inertness • Stable from pH 1.5 to 10.5* for Unmatched Versatility and Stability • Manufactured under ISO-9000 Conditions for Consistent High Performance • Easy Scale Up from Analytical to Preparative • Unconditional 90-Day Guarantee No matter what your separation challenge, an Alltech Apollo™ HPLC Column will solve the problem. Apollo™ packings are made with high-purity spherical silica and consistent bonding techniques, yielding a reproducible surface that's inert toward all analytes, including acidic and basic compounds (Figure 1). This same combination affords exceptional pH stability (from 1.5 to 10.5)* - well beyond that of standard reversed-phase columns. And Alltech's ISO-9000 certified manufacturing system yields columns with exceptional efficiency and consistent selectivity time after time! Apollo™ Columns’ separation power meets or exceeds other premium HPLC column brands, and at a price/performance ratio that can't be beaten. Apollo™ HPLC Columns are also available in preparative formats. Develop your separation on an Apollo analytical column to determine optimum mobile phase conditions and loading limits, then scale up to 10 or 22mm columns based on your needs.

Apollo™ HPLC Columns PHASE

PARTICLE SIZE

LENGTH X ID

INDUSTRY STANDARD PART NO.

C18

5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

150 x 4.6mm 250 x 4.6mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm

36505 36511 36530 36541 36531 36543 36526 36537

36515 36521 — — — — — —

Phenyl

5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

150 x 4.6mm 250 x 4.6mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm

36538 36544 36525 36539 36527 36532 36535 36528

36536 36545 — — — — — —

Silica

5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

150 x 4.6mm 250 x 4.6mm 150 x 7mm 250 x 7mm 150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm

36507 36513 36542 36529 36533 36540 36534 36524

36517 36523 — — — — — —

In addtion, Alltech has more than 20-years experience manufacturing HPLC columns. We'll help you get the most from your chromatography purchase by carrying extensive inventory, supplying prompt, free, expert technical support, and by backing your Apollo™ Column with an unconditional 90-day money-back guarantee. *Alltech Associates, Inc. does not recommend using any silica-based HPLC column outside the pH range of 2-7.5 for prolonged periods. When pH extremes are necessary, use non-phosphate modifiers such as TFA (acidic pH) or pyrrolidine hydrochloride (basic pH).

Apollo Characteristics C18 Phenyl Silica

PARTICLE SIZE

PARTICLE SHAPE

CARBON LOAD

ENDCAPPED?

5µm 5µm 5µm

Spherical Spherical Spherical

15 8 —

Yes Yes —

PRICE

Apollo™ Guard Columns DESCRIPTION

C18 C18-Prep* Phenyl Silica Silica-Prep*

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

5µm 5µm 5µm 5µm 5µm

7.5 x 4.6mm 33 x 7mm 7.5 x 4.6mm 7.5 x 4.6mm 33 x 7mm

3 1 3 3 1

96454 970205 96430 96419 970206

PRICE

Guard Holders (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder Ea 80101 *No guard holder required for prep guards.

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hplc hplc

PHASE

WATERS INSTRUMENT PART NO.

376

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech Apollo Acids

Bases 9267

9266

1. Maleic Acid 2. Phenylbutyric Acid 3. Phenylvaleric Acid

1

1. 2. 3. 4.

Ethylpyridine Aniline Benzaldehyde N,N-Diethyl-mtoluamide (DEET) 5. Dimethylaniline

3 5

2

0

1

2

Column: Mobile Phase: Flowrate: Detector:

3

3

2

1

4

0

5 Min.

Apollo™ C18, 5µm, 150 x 4.6mm CH3CN:50mM KH2PO4 pH 3.0 (60:40) 1.0mL/min UV at 254nm

1

4

2

3

5 Min.

4

Apollo™ C18, 5µm, 150 x 4.6mm CH3CN:50mM KH2PO4 pH 3.0 (60:40) 1.0mL/min UV at 254nm

Column: Mobile Phase: Flowrate: Detector:

Figure 1 - Alltech Apollo™ HPLC Columns exhibit excellent peak-shape and selectivity for both acids and bases.

Acids and Bases

Parabens 4

8740

8 Min.

Column: Apollo C18, 5µm, 150x 4.6mm Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 7 %B: 60 75 Flowrate: 1.0mL/min Detector: Alltech ELSD

Anticonvulsants 7233

1. Cortisone Degradant 2. Hydrocortisone-21acetate Degradant 3. Cortisone Degradant 4. Hydrocortisone-21acetate Degradant 5. Cortisone 6. Hydrocortisone-21acetate

16

20 Min.

123

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4

Ethotoin Phenobarbital Methsuximide Mephenytoin Carbamazepine Diazepam

10

15

20 Min.

0

4

8

12

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5

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Contact your Alltech office or distributor for current or local prices.

Column: Apollo C18, 5µm, 250 x 4.6mm Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 20 25 30 %B: 15 60 60 15 Flowrate: 1.5mL/min Detector: UV at 254nm © Copyright 2003 Alltech Associates, Inc.

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Apollo C18, 5µm, 150x 4.6mm 50mM KH2PO4, pH2.75:Acetonitrile (65:35) 1.0mL/min UV at 242nm

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Column: Mobile Phase: Flowrate: Detector:

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34

1. 2. 3. 4. 5. 6.

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2 1

6

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9026

5

hplc hplc

5

6

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Column: Apollo C18, 5µm, 150x 4.6mm Mobile Phase: Acetonitrile:0.05M Potassium Phosphate Buffer, pH3.0 (45:55) Flowrate: 1.0mL/min Detector: UV at 254nm

Steroids

7 Min.

1

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6

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4

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2

1

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3

1. Methylparaben 2. Ethylparaben 3. Propyl Paraben 4. Butyl Paraben

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1

0

2

4 1. N,N-Diethylaniline 2. Phenylacetic Acid 3. N,N-Dimethylaniline 4. Phenylvaleric Acid

2

9201

3

377

Alltech alphaBond™ Maintain µBondapak® Performance and Save Money with alphaBond™

USP XXII Acetaminophen and Aspirin Tablets Method µBondapak® C18

alphaBond™ C18

• Equivalent Selectivity, Peak Symmetry, Capacity, and Efficiency • Available in 5 and 10µm Particle Sizes • Excellent Column-to-Column Reproducibility • Reasonably Priced

1

1

7671

7670

Alltech alphaBond™ was developed to provide chromatographers with a superb alternative to Waters µBondapak®. Through exhaustive research Alltech has developed a packing which not only meets the physical characteristics of µBondapak®, but chromatographically performs as a true equivalent, even for USP applications.

3

™

We ran many difficult USP applications on alphaBond , all of which met the system suitability requirements. Additionally, we investigated many different probes specifically representing acidic, basic, and neutral compounds. The results indicate comparable performance regarding capacity, selectivity, efficiency, and peak symmetry when compared directly to µBondapak®. While µBondapak® is only available in 10µm particle size, alphaBond comes in both 5 and 10µm sizes. Columns packed with 5µm particles provide higher efficiency for difficult-to-separate compounds. Alltech has very rigorous manufacturing guidelines which minimize lot-to-lot variation. Additionally, Alltech maintains a strict QC program for column production, demonstrating excellent column-to-column reproducibility. Alltech alphaBond™ is available with Waters instrument type endfittings as well as the industry standard endfittings.

3 4

4

2 0

2

2

4

6

8

10 Min.

0

2

4

6

8

10 Min.

1. Acetaminophen 2. Salicylic Acid 3. Aspirin 4. Benzoic Acid Column: Mobile Phase:

Flowrate: Detector:

10µm, 300 x 3.9mm 0.225% Tetramethylammonium Hydroxide Pentahydrate in a mixture of Water: Methanol:Acetonitrile:Acetic Acid (75:12.5:12.5:0.1) 2mL/min UV 280

alphaBond™ C18 gives equivalent peak shape as µBondapak® C18 for all components, including the acids.

alphaBond™ Columns LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

5µm 10µm 10µm

150 x 3.9mm 150 x 3.9mm 300 x 3.9mm

77071 77022 77023

77072 77020 77021

C8

10µm 10µm

150 x 3.9mm 300 x 3.9mm

77043 77044

77051 77052

Phenyl

10µm 10µm

150 x 3.9mm 300 x 3.9mm

77045 77046

77053 77054

Cyano

10µm 10µm

150 x 3.9mm 300 x 3.9mm

77047 77048

77055 77056

PARTICLE SIZE

C18

Packing Characteristics Comparison PRICE

µBONDAPAK® C18

Packing Shape: Particle Size: Pore Size: Surface Area: Carbon Load: Endcapped:

ALPHABOND™

Irregular 10µm 125Å 300m2/g 10% Yes

C18

Irregular 5, 10µm 125Å 300m2/g 10% Yes

alphaBond™ Guard Columns DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

Amino

10µm 10µm

150 x 3.9mm 300 x 3.9mm

77049 77050

77057 77058

Silica

5µm 10µm 10µm

150 x 3.9mm 150 x 3.9mm 300 x 3.9mm

77077 77031 77032

77078 77029 77030

i

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

All-Guard™ Cartridges (Requires Guard Holder) C18 5µm 7.5 x 4.6mm 3 77082 C18 10µm 7.5 x 4.6mm 3 96086 C8 10µm 7.5 x 4.6mm 3 77059 Phenyl 10µm 7.5 x 4.6mm 3 77060 Cyano 10µm 7.5 x 4.6mm 3 77061 Amino 10µm 7.5 x 4.6mm 3 77062 Silica 5µm 7.5 x 4.6mm 3 77083 Silica 10µm 7.5 x 4.6mm 3 96087 Guard Holders (Includes Direct-Connect Column Coupler) All-Guard™ Cartridge Holder Ea 80101

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hplc hplc

PACKING

378

© Copyright 2003 Alltech Associates, Inc.

i

For complete details on Alltech All-Guard Columns, see pages 460-464. Contact your Alltech office or distributor for current or local prices.

Alltech Carbohydrate Carbohydrate Amino Phase

Corn Syrup 3

1

Alltech has developed an amino bonded phase with the same selectivity as regular aminopropyl phases but with a drastically improved lifetime. A highly crosslinked 10 micron amino bonded phase provides exceptional resistance against the hydrolysis exhibited by regular amino phases. The reactivity toward aldehydes and ketones is eliminated with the addition of 0.1% of Alltech’s column lifetime extension agent to the mobile phase (5g bottle included with each column).

5615

1. Water 2. Pentose 3. Fructose 4. Disaccharide 4

2

OH-100 Anomer Analysis Column Alltech’s Carbohydrate OH-100 column is designed specifically for the determination of anomeric forms of sugars. The OH-100 column consists of a silica-based polyol matrix which is inherently more stable than amino bonded phases and possesses the same versatility with respect to altering selectivity via mobile phase composition. The polyol phase interacts more strongly with the cis- anomers than with the trans-, thus providing a means for separation. Sugar alcohols and other carbohydrates which do not form anomers are eluted as single peaks.

0 Column: Mobile Phase: Flowrate: Temp: Detector:

6 12 Min.

Alltech Carbohydrate Amino, 10µm, 300 x 4.1mm Acetonitrile: Water (85:15) 3.0mL/min 25°C RI

Sugars and Sugar Alcohols

Carbohydrate Cation Exchange Column

5260

8

The Alltech Carbohydrate Cation Exchange Column consists of a highly efficient sulfonated polystyrene resin supplied in the calcium form. The cation column provides excellent separation using only water as the mobile phase. Analytical columns come with industry standard fittings and dimensions of 300 x 6.5mm.

2 5 6 1

1. 2. 3. 4. 5. 6. 7. 8.

7

34

Carbohydrate Columns WATERS INSTRUMENT PART NO.

Carbohydrate Amino 10µm

300 x 4.1mm

35641

35642

OH-100 Anomer 7µm

250 x 4.6mm

35541

35542

Carbohydrate Cation Exchange 10µm 300 x 6.5mm

70057

—

PRICE

0

2

4

Column: Mobile Phase: Flowrate: Temp: Detector:

Alltech Cation Exchange, 10µm, 300 x 6.5mm Water 0.5mL/min 90°C RI

PART NO.

PRICE

8965

3

Guard Holders (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder Ea 80101

2

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1

5

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35644

0

5

10

15 Min.

Contact your Alltech office or distributor for current or local prices.

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Also consider the high performance Prevail™ Carbohydrate ES Column on page 400-401.

Alltech Cation Exchange, 10µm, 300 x 6.5mm Water 0.5mL/min 90°C Alltech ELSD © Copyright 2003 Alltech Associates, Inc.

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Column: Mobile Phase: Flowrate: Temp: Detector:

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Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

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Accessories Column Lifetime Extension Agent, 5 grams Ea

Unknown Sucrose Glucose Fructose Sorbitol

○

1. 2. 3. 4. 5.

96107 96113 96109

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4

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QTY

All-Guard™ Cartridges (Requires Guard Holder) Amino Carbohydrate 10µm 7.5 x 4.6mm 3 OH-100 Anomer 7µm 7.5 x 4.6mm 3 Carbohydrate Cation 5µm 7.5 x 4.6mm 3

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LENGTH X ID

10 12 14 16 Min.

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PARTICLE SIZE

8

100% Pure Red Delicious Apple Juice (a 1:250 Dilution)

Guard Columns and Accessories DESCRIPTION

6

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PACKING

hplc hplc

LENGTH X ID

INDUSTRY STANDARD PART NO.

PARTICLE SIZE

Maltotriose Sucrose Glucose Galactose Fructose Mannitol Arabitol Sorbitol

379

Alltech Econosil™ Econosil™ HPLC Columns

Econosil™ Preparative Columns

• Low-cost, Quality Columns • Totally Porous, Irregularly-shaped Packing • Five or Ten Micron Particle Sizes • Preparative Columns Available for Easy Scale-up

Econosil™ preparative scale columns are available as stock items. Develop your separation on an Econosil™ Analytical Column to determine the optimum mobile phase conditions and loading limits. Then choose the preparative column dimensions based on the quantity of material you wish to analyze. The 7mm, 10mm, Econosil™ is an irregularly-shaped silica packing with a high surface 22mm, and 50mm ID columns will separate approximately 2X, 5X, area and carbon load. The silica is acid washed to remove trace metals 20X, and 120X more material, respectively, than 4.6mm ID and then polymerically bonded with either C8, C18, CN or NH2. The analytical columns. material is then endcapped (except NH2) to derivatize any unreacted silanols, minimizing the need for mobile phase modifiers. Columns Econosil™ Preparative Columns are available in standard dimensions as well as in preparative formats. PARTICLE INDUSTRY STANDARD As with all Alltech packings, if you need special column dimensions, PACKING SIZE LENGTH X ID PART NO. PRICE see the Custom Packed HPLC section of this catalog. C18 10µm 250 x 7mm 6215 ™ Although Econosil Columns are low-cost, the quality requirements 10µm 250 x 10mm 6231 for these columns are equal to other Alltech columns. We strongly 10µm 250 x 22mm 6251 recommend that every column be tested in your lab as soon as 10µm 250 x 50mm 6318 possible after you receive it. This is to insure that no damage occurred during shipment and that the column arrived in optimal C8 10µm 250 x 7mm 6223 condition. As with all our columns we offer a 90 day limited warranty. 10µm 250 x 10mm 6239 10µm 250 x 22mm 6253 Econosil™ Specifications 10µm 250 x 50mm 6321 Surface Area: 450m2/g Pore Size: 60Å CN 10µm 250 x 7mm 6219 Carbon Load, C18: 15% 10µm 250 x 10mm 6235 10µm 250 x 22mm 6255 Carbon Load, C8: 10% 10µm 250 x 50mm 6323 Particle Sizes: 5- or 10µm

Econosil™ Analytical Columns

hplc hplc ○

10µm 10µm 10µm 10µm

250 x 7mm 250 x 10mm 250 x 22mm 250 x 50mm

6221 6237 6257 6325

10µm 10µm 10µm 10µm

250 x 7mm 250 x 10mm 250 x 22mm 250 x 50mm

6217 6233 6259 6327

LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

5µm 5µm 5µm 10µm

150 x 3.2mm 250 x 3.2mm 250 x 4.6mm 250 x 4.6mm

60017 60035 60131 60147

60019 60027 60132 60148

Silica

5µm 5µm 5µm 10µm

150 x 3.2mm 250 x 3.2mm 250 x 4.6mm 250 x 4.6mm

60029 60037 60134 60150

60031 60033 60135 60151

Econosil™ Prep Guard Columns

CN

5µm 10µm

250 x 4.6mm 250 x 4.6mm

60137 60153

60138 60154

NH2

5µm 10µm

250 x 4.6mm 250 x 4.6mm

60141 60156

60142 60157

Silica

5µm 10µm

250 x 4.6mm 250 x 4.6mm

60144 60159

60145 60161

PARTICLE SIZE

C18

C8

PACKING

PRICE

DESCRIPTION

PARTICLE SIZE

LENGTH X ID

10µm 10µm

33 x 7mm 33 x 7mm

C18 Silica

1

QTY

PART NO.

PRICE

1 970207 1 970208

Aromatic Ketones

2

5457

3

1. Acetophenone 2. Benzophenone 3. Chalkone 4. β-Phenylchalkone

4

Econosil™ Guard Columns DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

0

™

All-Guard Cartridges (Requires Guard Holder) C18 10µm 7.5 x 4.6mm 3 C18 5µm 7.5 x 3.2mm 3 C8 10µm 7.5 x 4.6mm 3 C8 5µm 7.5 x 3.2mm 3 CN 10µm 7.5 x 4.6mm 3 NH2 10µm 7.5 x 4.6mm 3 Silica 10µm 7.5 x 4.6mm 3

96115 96363 96117 96364 96120 96119 96126

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NH2

380

Guard Holders (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder Ea 80101 © Copyright 2003 Alltech Associates, Inc.

4

Column: Mobile Phase: Flowrate: Detector:

i i

8

12 Min.

Econosil C18, 10µm, 250 x 4.6mm Methanol: Water (70:30) 1.0mL/min UV at 254nm

Other dimensions and formats available. See pages 355-361 for custom HPLC columns. See page 359 for Custom Preparative Columns.

Contact your Alltech office or distributor for current or local prices.

Alltech Econosphere™ Econosphere™ HPLC Columns

Econosphere™ Rocket™ Columns DESCRIPTION

• High-Performance, Low Cost Columns • Preparative Columns Available for Easy Scale-up

PARTICLE SIZE

LENGTH X ID

PART NO.

3µm 3µm

53 x 7mm 33 x 7mm

50675 50673

Econosphere™ C18

Econosphere™ is a spherical silica, that is acid washed to remove trace metals, monomerically bonded with either C8, C18, CN, or NH2, and endcapped to minimize mixed-mode retention and adsorption. Use an Econosphere™ column as a “work horse” for a wide variety of separations. Dedicate a low cost Econosphere™ Column to a single application to avoid mobile phase induced changes in column selectivity. Econosphere™ packings are available in 3µm, 5µm, and 10µm particles, packed in analytical, semi-preparative, and preparative formats. Although Econosphere™ Columns are low-cost, the quality requirements for these columns are equal to other Alltech columns. We strongly recommend that every column be tested in your lab when you receive it. This is to insure that no damage occurred during shipment and that the column arrived in optimal condition.

PRICE

Econosphere™ Guard Columns DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

All-Guard™ Cartridges (Requires Guard Holder) C18 5µm 7.5 x 3.2mm 3 96365 C18 5µm 7.5 x 4.6mm 3 96121 C8 5µm 7.5 x 3.2mm 3 96366 C8 5µm 7.5 x 4.6mm 3 96122 CN 5µm 7.5 x 4.6mm 3 96123 NH2 5µm 7.5 x 4.6mm 3 96124 Silica 5µm 7.5 x 4.6mm 3 96125 Guard Holders (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder Ea 80101

Econosphere™ Specifications

Econosphere™ Preparative Columns

200m2/gram 80 angstrom 0.8mL/gram 10% 5% 3, 5, and 10µm

Develop your separation on an Econosphere™ Analytical Column to determine the optimum mobile phase conditions and loading limits. Then choose the preparative column dimensions based on the quantity of material you wish to analyze. The 10mm and 22mm ID columns will separate approximately 5X and 20X more material, respectively, than 4.6mm ID analytical columns.

Econosphere™ Preparative Columns

70156 70157 70158 70112 70070 70102 70071

3µm 3µm 3µm 5µm 5µm 5µm

50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 3.2mm 150 x 4.6mm 250 x 3.2mm

70165 70166 70167 70107 70085 70114

70168 70169 70170 70108 70090 70104

CN

5µm 5µm

150 x 4.6mm 250 x 4.6mm

70025 70026

70030 70031

NH2

5µm 5µm

150 x 4.6mm 250 x 4.6mm

70045 70046

70050 70051

C8

10µm 10µm 10µm 10µm

100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm

28159 28136 28087 28003

NH2

10µm 10µm 10µm 10µm

100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm

28161 28137 28091 28005

CN

10µm 10µm 10µm 10µm

100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm

28163 28138 28093 28007

Silica

10µm 10µm 10µm 10µm

100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm

28167 28156 28095 28057

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C8

28157 28097 28085 28001

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70153 70154 70155 70109 70065 70113 70066

100 x 10mm 100 x 22mm 250 x 10mm 250 x 22mm

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50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 3.2mm 150 x 4.6mm 250 x 3.2mm 250 x 4.6mm

10µm 10µm 10µm 10µm

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3µm 3µm 3µm 5µm 5µm 5µm 5µm

C18

C18

PRICE

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70180 70181 70182 70010 70011

PRICE

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70177 70178 70179 70005 70006

INDUSTRY STANDARD PART NO.

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50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

Silica

LENGTH X ID

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3µm 3µm 3µm 5µm 5µm

PACKING

PARTICLE SIZE

PACKING

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hplc hplc

LENGTH X ID

INDUSTRY STANDARD PART NO.

PARTICLE SIZE

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Econosphere™ Columns

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Surface Area: Pore Diameter: Pore Volume: Carbon Load, C18: Carbon Load, C8: Particle Sizes:

10µm 10µm

33 x 7mm 33 x 7mm

PART NO.

PRICE

1 970209 1 970210

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C18 Silica

QTY

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Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

LENGTH X ID

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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PARTICLE SIZE

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DESCRIPTION

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Econosphere™ Prep Guard Columns

381

Alltech Econosphere™ (ARG8)-Vasopressin

Sugars 5464

5466

2

1

1

1. 2. 3. 4. 5. 6. 7. 8.

3 4 56 7

0

8

10 Min.

5

Column: Mobile Phase: Flowrate: Detector:

1. (ARG8)-Vasopressin

Xylose Fructose Glucose Sucrose Maltose Lactose Melezitose Raffinose

0 3 6 Min. Column: Econosphere™ C18, 5µm, 250 x 4.6mm Mobile Phase: Water (0.5% H3PO4 + 0.15% TEA):Acetonitrile (80:20) Flowrate: 2.0mL/min Detector: UV at 254nm

Econosphere™ NH2, 5µm, 250 x 4.6mm Acetonitrile:Water (75:25) 4.0mL/min RI

Phenols

Anticonvulsants 7 2

3

PEMA Primidone Phenobarbital Carbamazepine–10,11epoxide 5. Hexobarbital 6. Diphenylhydantoin 7. Carbamazepine

4

1

0049

1. 2. 3. 4. 5 6

5 1 2

4

2

6

8 10 12 Min.

hplc hplc

Column: Mobile Phase: Flowrate: Detector:

Econosphere™ C18, 5µm, 150 x 4.6mm 0.1M KH2PO4, pH 4.4:Methanol:TEA (61:39:0.01) 2.0mL/min UV at 210nm

6

7

8

4

8

12

16 Min.

Column: Econosphere™ C8, 5µm, 150 x 4.6mm Mobile Phase: A: Water + 1% Acetic Acid B: Methanol + 1% Acetic Acid Gradient: Time: 0 15 %B: 40 100 Flowrate: 1.5mL/min Detector: UV at 280nm

PTC Amino Acids

4

Polyethylene Glycol 400 7810

5164

10

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3 ○

7 8 6

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1

5

Aspartic Acid Glutamic Acid Serine Glycine Histidine Threonine Alanine Arginine

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2

1. 13 2. 15 3. 11 14 4. 12 5. 17 6. 7. 9 8. 16

0

10

20

30

40

50

9. 10. 11. 12. 13. 14. 15. 16. 17.

Proline Tyrosine Methionine Valine Cysteine Isoleucine Leucine Phenylalanine Lysine

60 Min.

0

5

10

15

20 Min.

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4

9

0 0

5471

1. Phenol 2. 4-Nitrophenol 3. 2,4-Dinitrophenol 4. o-Chlorophenol 5. 2-Nitrophenol 6. 2,4-Dimethylphenol 7. 4-Chloro-3-methylphenol 10 8. 2,4-Dichlorophenol 9. 2,4,6-Trichlorophenol 10. Pentachlorophenol

3

382

Column: Econosphere™ C8, 5µm, 250 x 4.6mm Mobile Phase: A: 50mM Ammonium Acetate, pH6.8 B: 100mM Ammonium Acetate, pH6.8:Methanol (20:80) Gradient: Time: 0 12 30 37 %B: 0 20 30 60 Flowrate: 2.0mL/min Detector: UV at 210nm © Copyright 2003 Alltech Associates, Inc.

Column: Mobile Phase: Flowrate: Detector:

Econosphere™ C8, 5µm, 250 x 4.6mm Methanol:Water (40:60) 1.0mL/min Alltech ELSD

Contact your Alltech office or distributor for current or local prices.

Alltech Macrosphere™ Size Exclusion Proteins

Macrosphere™ Columns

6

• Separate Proteins, Peptides and Polar Synthetic Compounds by Size Exclusion • Maintains Biological Activity • Seven Controlled Pore Sizes

4 5

1

0

Macrosphere GPC Exclusion Limits in Daltons (Data for Proteins) PORE SIZE

INCLUDED

EXCLUDED

60Å 100Å 150Å 300Å 500Å 1000Å 4000Å

250 2500 4000 7500 20,000 30,000 —

28,000 350,000 500,000 1,200,000 7,500,000 25,000,000 —

1. 2. 3. 4. 5. 6.

2 3

The Macrosphere™ series separates proteins, peptides, and other water soluble macromolecules by size exclusion chromatography. By bonding silica with a hydrophilic (diol) layer, the support is rendered inert to proteins when using a buffered mobile phase. Macrosphere™ GPC is available in seven pore sizes (60-, 100-, 150-, 300-, 500-, 1000-, 4000-angstrom) to accommodate any molecular weight range. For samples with a range of molecular weights, two or more columns with different pore sizes may be joined in series.

™

5192

5

10

15

20

Thyroglobulin Ferritin Transferrin β-Lactoglobulin Myoglobin Glycyl-L-Tyrosine

25 Min.

Macrosphere™ GPC 150/300/500Å, 7µm, 250 x 4.6mm (3) Mobile Phase: 0.05M K2HPO4 containing 0.15M Na2SO4, pH7.0 Flowrate: 0.3mL/min Detector: UV at 280nm Column:

Proteins 9015

1

1. Alpha-Amylase 2. Alpha-Lactalbumin

2

Macrosphere™ Columns

88130 — 88134 — 88138 — 88142 — 88146 — 88150 — 88154 —

PRICE

0

2

Column: Mobile Phase: Flowrate: Detector:

4

6

8 Min.

™

Macrosphere GPC 100Å, 250 x 4.6mm 25mM K2HPO4, pH 7.0 0.5mL/min UV at 220nm

Proteins 1 2

5191

Macrosphere™ GPC Guard Columns LENGTH X ID

QTY

PART NO.

1. 2. 3. 4.

3

PRICE

○

All-Guard™ Cartridges (Requires Guard Holder) Custom 7.5 x 4.6mm 4 C-9800 Specify Material and Particle Size

Enolase Cytochrome Myokinase Impurity

4

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DESCRIPTION

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88129 88175 88133 88177 88137 88179 88141 88181 88145 88183 88149 88185 88153 88187

○

LENGTH X ID

250 x 4.6mm 300 x 7.5mm 250 x 4.6mm 300 x 7.5mm 250 x 4.6mm 300 x 7.5mm 250 x 4.6mm 300 x 7.5mm 250 x 4.6mm 300 x 7.5mm 250 x 4.6mm 300 x 7.5mm 250 x 4.6mm 300 x 7.5mm

○

7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm

WATERS INSTRUMENT PART NO.

hplc hplc

60Å 60Å 100Å 100Å 150Å 150Å 300Å 300Å 500Å 500Å 1000Å 1000Å 4000Å 4000Å

INDUSTRY STANDARD PART NO.

○

GPC

PARTICLE SIZE

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PACKING

PORE SIZE

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Guard Holders (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder Ea 80101 Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

0

4

i

For complete details on Alltech All-Guard Columns, see pages 460-464.

Column: Mobile Phase: Flowrate: Detector:

Macrosphere™ GPC 300Å, 7µm, 250 x 4.6mm 0.1M K2HPO4 & 0.2M NaCl pH7.0 0.3mL/min UV at 280nm © Copyright 2003 Alltech Associates, Inc.

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12 Min.

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8

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Contact your Alltech office or distributor for current or local prices.

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i

383

Alltech Macrosphere™ Reversed Phase and Ion Exchange Macrosphere™ Ion-Exchange Columns

Macrosphere™ Reversed-Phase Columns

• 300Å Weak or Strong Anion and Cation Exchange Supports for Biomolecule Separations

• Reversed-Phase Separation of Proteins, Peptides and Oligonucleotides • Optimized Bonding Chemistry The Macrosphere™ Reversed Phase series includes C4, C8, and C18 supports for the rapid reversed-phase separation of proteins and peptides. These spherically-shaped, mechanically-stable materials are exhaustively bonded to minimize residual silanol activity for optimum protein retention and recovery. Typical protein recoveries are greater than 90%. The choice of three alkyl chain lengths provides a valuable method for altering selectivity between proteins and small hydrophobic molecules in the same matrix. The Macrosphere™ series of supports are available in 5 and 7 micron particle sizes to accommodate applications requiring mobile phase systems with varying degrees of viscosity. Macrosphere™ Reversed-Phase Solvent Miser (2.1mm ID) columns offer small sample requirements, increased mass sensitivity and reduced solvent consumption without the need for special HPLC instrumentation. ™

Macrosphere Reversed-Phase Columns LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

150 x 4.6mm 250 x 2.1mm 250 x 4.6mm 250 x 4.6mm

71035 72224 71039 71047

71036 — 71040 71048

5µm 5µm 5µm 7µm

150 x 4.6mm 250 x 2.1mm 250 x 4.6mm 250 x 4.6mm

71054 72228 71057 71063

71055 — 71058 71064

5µm 5µm 5µm 7µm

150 x 4.6mm 250 x 2.1mm 250 x 4.6mm 250 x 4.6mm

71069 72232 71072 71078

71070 — 71073 71079

PORE SIZE

PARTICLE SIZE

300Å 300Å 300Å 300Å

5µm 5µm 5µm 7µm

C8

300Å 300Å 300Å 300Å

C18

300Å 300Å 300Å 300Å

PACKING

C4

hplc hplc ○ ○

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

All-Guard™ Cartridges (Requires Guard Holder) C4 300Å 5µm 7.5 x 3.2mm 3 96379 C4 300Å 5µm 7.5 x 4.6mm 3 96131 C8 300Å 5µm 7.5 x 3.2mm 3 96378 C8 300Å 5µm 7.5 x 4.6mm 3 96129 C18 300Å 5µm 7.5 x 3.2mm 3 96377 C18 300Å 5µm 7.5 x 4.6mm 3 96127 Guard Holders (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder Ea 80101

i

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

i

For complete details on Alltech All-Guard Columns, see pages 460-464.

ANION EXCHANGE WAX, SAX

CATION EXCHANGE WCX, SCX

Bonded Phase Functionality: Diethylaminoethane (WAX) Quaternary Ammonium (SAX)

Carboxylic Acid (WCX) Sulfonic Acid (SCX)

Mobile Phase Exchange Species: Acetate Phosphate Sulfate

Sodium Potassium Ammonium

Ion Exchange Capacities PHASE

EXCHANGE CAPACITY (MEQ/GRAM)

WAX, 7µm, 300Å WCX, 7µm, 300Å SAX, 7µm, 300Å SCX, 7µm, 300Å

0.13 0.49 0.14 0.50

Macrosphere™ Ion Exchange Columns PACKING

PRICE

PORE SIZE

WAX 300Å 300Å WCX 300Å 300Å SAX 300Å 300Å SCX 300Å 300Å

PARTICLE SIZE

7µm 7µm 7µm 7µm 7µm 7µm 7µm 7µm

LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

71151 71154 71157 71160 72303 72603 72308 72608

71152 71155 71158 71161 72304 72604 72309 72609

PRICE

Macrosphere™ Ion Exchange Guard Columns DESCRIPTION

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

PRICE

All-Guard™ Cartridges (Requires Guard Holder) WAX 7µm 7.5 x 4.6mm 3 96135 WCX 7µm 7.5 x 4.6mm 3 96137 SAX 7µm 7.5 x 4.6mm 3 96139 SCX 7µm 7.5 x 4.6mm 3 96141 Guard Holders (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder Ea 80101

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PORE SIZE

Ion Exchange Mechanisms

PRICE

Macrosphere™ Reversed-Phase Guard Columns DESCRIPTION

The Macrosphere™ Ion Exchange supports are weak and strong anion and cation exchangers for the analysis of proteins, enzymes, nucleosides, nucleotides, and other biological molecules. The Macrosphere™ Ion-Exchange series includes weak anion (WAX), weak cation (WCX), strong anion (SAX), and strong cation (SCX) exchange supports. Due to the nature of the bonded phases, the Macrosphere™ Ion Exchangers have elution protocols which are similar to those of soft-gel ion-exchange supports (DEAE, CM, SA, and SB).

384

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Alltech Mixed-Mode™ Mixed-Mode™ Columns

Anesthetics and Neutral

• Solve Tough Separation Problems • Reversed-Phase and Ion-Exchange Capability In One Column! • Eliminates the Need for Ion-Pair Reagents and Amine Modifiers for Separations of Basic/Nitrogen Containing Compounds

2

2

The Alltech Mixed-Mode™ Columns use multifunctional supports for separating hydrophobic as well as ionic species. The support consists of a 100 angstrom, spherical silica substrate bonded with either anionic (carboxylate) or cationic (amine) functionalities and conventional reversed-phase (C8, C18) functionalities. Difficult applications which previously required special conditions (ion-pair reagents or base-deactivated supports) can be developed by controlling ionization via mobile phase pH adjustments, buffer strength and organic modifier composition. As demonstrated in Figure 1, changing the buffer concentration affects the capacity of the cationic components, lidocaine and procaine, but does not affect the capacity of biphenyl, a neutral component.

1

3

8780

8781

0

PACKING

C8/Anion

LENGTH X ID

WATERS INSTRUMENT PART NO.

71355 12370

C8/Cation 100Å 5µm 150 x 4.6mm 72568 100Å 5µm 250 x 4.6mm 71364

72569 71365

C18/Cation 100Å 100Å 100Å 100Å

72574 71369 71374 12459

72575 71370 71375 12460

LENGTH X ID

QTY

PART NO.

7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm

3 3 3 3

96147 71379 71380 96145

Ea

80101

5µm 5µm 7µm 7µm

150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

10

15

20 Min.

PRICE

Figure 1 - Changing the buffer concentration affects the capacity of the cationic components, lidocaine and procaine, but does not affect the capacity of biphenyl, a neutral component.

Acids 1. 4-Hydroxy-3-methoxy-mandelic Acid 2. Acetylsalicylic Acid 3. Phenol 2

9096

hplc hplc

100Å 7µm 150 x 4.6mm 71354 100Å 7µm 250 x 4.6mm 12369

5

Column: Mixed-Mode™ C8/Cation, 5µm, 150 x 4.6mm Mobile Phase 8780: 55mM K2HPO4, pH6.5: Acetonitrile (50:50) 8781: 45mM K2HPO4, pH6.5: Acetonitrile (50:50) Flowrate: 1.0mL/min Temp: 30°C Detector: UV at 220nm

Mixed-Mode™ Analytical Columns INDUSTRY STANDARD PART NO.

3 1

For the reversed-phase anion exchange phases, the amine functions are protonated at acidic pH’s, increasing the anion exchange character of the support. For the reversed-phase cation exchange phases, pH’s above 4 promote ionization of the carboxylic functions and increased cation exchange character. The 100Å pore size supports are suitable for the separation of tricyclic antidepressants, CNS stimulants, anesthetics, anticonvulsants, benzodiazepines and other similarly sized molecules.

PORE PARTICLE SIZE SIZE

1. Biphenyl 2. Lidocaine 3. Procaine

Mixed-Mode™ Guard Columns* 1

PRICE

○

3 ○

PARTICLE SIZE

All-Guard™ Cartridge Holder

○

○

All-Guard™ Cartridges C8/Anion 7µm C8/Cation 5µm C18/Cation 5µm C18/Cation 7µm

○

DESCRIPTION

2

4

6

8 Min.

○ ○ ○ ○ ○ ○

© Copyright 2003 Alltech Associates, Inc.

○

Contact your Alltech office or distributor for current or local prices.

○

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

Mixed-Mode™ C8/Anion, 7µm, 150 x 4.6mm 30:70 Methanol:100mM K2HPO4, pH 7 1.0mL/min 30°C UV at 254nm

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i

Column: Mobile Phase Flowrate: Temp: Detector:

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*Requires Guard Holder

385

Alltech Organic Acid Columns Organic Acid Columns • Rapid Analysis of Organic Acids & Alcohols • pH Resistant Resin Packing • Short Analysis Times • Isocratic Aqueous Mobile Phases Only, No Organic Solvents

8

Short-Chain Aromatic Carboxylic Acids

10

7

2 4

OA-1000 and OA-2000

11 12

9

6

3

The OA-1000 and OA-2000 columns are ion exclusion columns packed with sulfonated polystyrene divinylbenzene. They exhibit excellent selectivity for aliphatic and aromatic acids. As with most ion exclusion columns, a column heater is necessary for normal operating procedures. The OA-1000 is 300mm in length for separation of inorganic anions such as fluoride, arsenate, sulfite, alcohols, and most organic acids. The OA-2000 is 100mm in length for separating organic acids with low pKa values, low molecular weight straight chain acids, and aromatic acids.

IOA-1000 and IOA-2000

5 1

0

10

20

Column: Mobile Phase: Flowrate: Temp: Detector:

The IOA-1000 and IOA-2000 columns are ion exclusion columns for separation of short-chain organic acids from glucose and fructose. The IOA-1000 is 300mm long and is an excellent column for the separation of acids of the Tri-Carboxylic Acid cycle (TCA or Krebs cycle). The IOA-2000 is 150mm long for fast separation of acids and some alcohols.

30 Min.

10

Alcohols

4

5440

8 5 6 10 2 3 7 9

1

4 7

10

20

Column: Mobile Phase: Flowrate: Temp: Detector:

30

40

50 Min.

IOA-1000, 300 x 7.8mm 0.005N Sulfuric Acid 0.3mL/min 60°C RI

12

1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Citric Acid Tartaric Acid Glucose Malic Acid Fructose Acetic Acid Glycerol Lactic Acid Methanol Ethanol

○ ○

5414

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1

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1. 2. 3. 4. 5.

5

8

10

0

2

4

6

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386

Column: Mobile Phase: Flowrate: Temp: Detector:

4

8

Maleic Acid Lactic Acid Acetic Acid Propionic Acid Butyric Acid

12

Column: Mobile Phase: Flowrate: Temp: Detector:

16

20

24

28 Min.

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Glycerol Methanol Ethanol iso-Propanol tert-Butanol Propanol sec-Butanol iso-Butanol tert-Pentanol Butanol iso-Pentanol Pentanol

OA-1000, 300 x 6.5mm 0.01N Sulfuric Acid 0.8mL/min 65°C RI

Organic Acid Columns PARTICLE SIZE

LENGTH X ID

PART NO.

OA-1000

9µm

300 x 6.5mm

9046

OA-2000

6.5µm

100 x 6.5mm

9048

IOA-1000

9µm

300 x 7.8mm

9646

IOA-2000

8µm

150 x 6.5mm

9648

PRICE

Organic Acid Guard Columns DESCRIPTION

QTY

PART NO.

All-Guard™ Cartridge Kit 1 Holder, 2 Cartridges

Ea

28883

Replacement Cartridges OA, 24 x 4.0mm

2

28884

PRICE

12 14 Min.

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34

0

PACKING

Organic Acids in Sour Cream

2

○

hplc hplc

0

9

8

11

5411

3 5

6

7067 Oxalic Acid Citric Acid Shikimic Acid Fumaric Acid Butyric Acid Homoprotocatechuic Acid Gallic Acid Protocatechuic Acid Gentisic Acid p-Hydroxybenzoic Acid Benzoic Acid Salicylic Acid

OA-2000, 100 x 6.5mm 0.01N Sulfuric Acid 0.8mL/min 45°C UV at 228nm

Wine Analysis by High Resolution Ion Exclusion

2 1

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

OA-1000, 300 x 6.5mm 0.01N Sulfuric Acid 0.8ml/min 65°C UV at 210nm

© Copyright 2003 Alltech Associates, Inc.

i

Also see our Silica-Based Prevail™ Organic Acid Column on page 402.

Contact your Alltech office or distributor for current or local prices.

Alltech Platinum™ Alltech Platinum™ HPLC Columns • Excellent Complement and Alternative to other Base-Deactivated Columns • Unique Interaction with Polar Compounds • Excellent Stability and Reproducibility Platinum™ and Platinum™ EPS (Extended Polar Selectivity) Columns are base-deactivated silica columns with a difference. The approach taken by most column manufacturers in making basedeactivated reversed-phase columns is to thoroughly cover the silica with bonded phase to minimize any interaction between polar analytes and the packing medium’s silica backbone. With our Platinum™ media, we have taken a different approach. Instead of maximizing the coverage of bonded phase to hide the silica, we have controlled the exposure of the silica to provide a dual mode separation medium with both polar and non-polar sites exposed to your samples. This extends polar selectivity well beyond what other reversed-phase columns offer and gives separations other columns can’t.

Controlled silica exposure also enhances the peak shape of difficult polar analytes. With conventional base-deactivated columns, the manufacturers minimize exposure of the underlying silica. Relatively few of the molecules making up a peak actually interact with these small pools of silica. Those that do interact with the silica elute slightly later than the main peak and cause tailing. By unmasking the silica and encouraging silanol interaction, all molecules comprising a peak now see a consistent environment which encourages symmetrical peak shape. Platinum™ Columns come in two varieties offering different levels of silica exposure. Standard Platinum™ has a moderate silica exposure and is best used with neutral and moderately polar compounds. Platinum™ EPS (Extended Polar Selectivity) has a high level of silica exposure and is best used with compounds containing more than two polar functional groups. Platinum™ columns succeed when other columns fail.

Most Base-Deactivated Columns Have the Same Selectivity, but Platinum™ Gives You a Choice Basic Pharmaceuticals On Competitive Base-Deactivated Silica Columns 2,3 8117

8121

2

2,3 1

1

Basic Pharmaceuticals on Platinum™ Columns 8118

N O T E

4

0

1

4

0

2 Min.

2,3

1

Standard Platinum and Platinum EPS often give different sample elution order.

Standard Platinum C18

Manufacturer 2

Manufacturer 1

1

2 Min.

3 4

0

1

2

3

4 Min.

2,3

8122

2 8120

N O T E 1

Highly polar compounds are retained longer on Platinum EPS than Standard Platinum.

○ ○ ○

3 4

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4

1

2 Min.

0

1

2 Min.

0

1

2

3

4 Min.

○ ○ ○

1. Procaine 2. Lidocaine 3. Tetracaine 4. Diphenhydramine

○

C18, 5µm, 100 x 4.6mm Methanol:0.025M K2HPO4, pH 7.0 (85:15) 2.0mL/min UV at 230nm

○ ○ ○ ○ ○ ○

The four chromatograms on the left all have the same peak elution order and the same coelution problem. The same separation on Standard Platinum™ has peak 3 well resolved and increased retention of peak 4. With Platinum ™ EPS, the peak elution order of peaks 1 and 2 is reversed, peak 4 is strongly retained, and all 4 components are baseline resolved. Contact your Alltech office or distributor for current or local prices. © Copyright 2003 Alltech Associates, Inc.

○

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Column: Mobile Phase: Flowrate: Detector:

○

○

0

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4

Platinum EPS C18

Manufacturer 4

Manufacturer 3

1

○

1

hplc hplc

8119

387

Alltech Platinum™ Platinum™ HPLC Columns PACKING

C18

Platinum™/Platinum™ EPS Media Specifications

PORE PARTICLE SIZE SIZE

LENGTH X ID

INDUSTRY STANDARD PART NO.

100Å 3µm 3µm 5µm 5µm 300Å 5µm 5µm 10µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm

32007 32020 32043 32064 32092 32097 32138

WATERS INSTRUMENT PART NO.

32009 32029 32044 32068 32093 32098 32139

100Å PACKINGS PRICE

Base Material: Particle Size: Pore Volume: Surface Area:

100Å 3µm 3µm 5µm 5µm 5µm 5µm 10µm 300Å 3µm 3µm 5µm 5µm 10µm

100 x 4.6mm 150 x 4.6mm 150 x 3.2mm 150 x 4.6mm 250 x 3.2mm 250 x 4.6mm 250 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm

32158 32183 32784 32214 32779 32246 32253 32258 32271 32278 32286 32303

32161 32184 32783 32216 32778 32247 32254 32259 32272 32279 32287 32328

C8

100Å 5µm 150 x 4.6mm 5µm 250 x 4.6mm

32370 32375

32371 32376

EPS C8

100Å 3µm 5µm 5µm 300Å 5µm 5µm

150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 150 x 4.6mm 250 x 4.6mm

32415 32420 32425 32446 32451

32416 32421 32426 32447 32452

C4

300Å 5µm 150 x 4.6mm 5µm 250 x 4.6mm

32471 32476

32472 32477

EPS C4

300Å 5µm 150 x 4.6mm 5µm 250 x 4.6mm

32496 32505

32497 32506

Phenyl

100Å 3µm 150 x 4.6mm 5µm 150 x 4.6mm 5µm 250 x 4.6mm

32631 32636 32641

32632 32637 32642

Spherical Silica 1.5-, 3-, 5-, 10-µm 0.51cc/g 200m2/g

PORE SIZE

PARTICLE SIZE

LENGTH X ID

Platinum Prep Guard Columns C18 100Å 10µ 33 x 7mm EPS C18 100Å 10µ 33 x 7mm Silica 100Å 10µ 33 x 7mm

hplc hplc

32672 32681

32675 32682

Amino

100Å 3µm 5µm 5µm 10µm

150 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm

32706 32713 32722 32732

32707 32714 32723 32733

100Å 3µm 150 x 4.6mm 5µm 150 x 4.6mm 5µm 250 x 4.6mm

32952 32944 32943

32953 32946 32945

100Å 3µm 150 x 4.6mm 5µm 150 x 4.6mm 5µm 250 x 4.6mm

32535 32542 32549

32536 32543 32550

i

Ea

970215* 970216* 970217* 80101

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

DESCRIPTION

PARTICLE SIZE

LENGTH X ID

PART NO.

C18

3µm 3µm 1.5µm 1.5µm

53 x 7mm 33 x 7mm 33 x 7mm 53 x 7mm

50523 50525 50527 50529

EPS C18

3µm 1.5µm 3µm 1.5µm

53 x 7mm 53 x 7mm 33 x 7mm 33 x 7mm

50573 50579 50575 50577

C8

1.5µm 3µm

53 x 7mm 33 x 7mm

50529 50532

EPS C8

3µm 3µm

53 x 7mm 33 x 7mm

50585 50583

NH2

3µm

53 x 7mm

50545

Cyano

3µm 3µm

53 x 7mm 33 x 7mm

50595 50593

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1 1 1

PRICE

○

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Silica

PRICE

Platinum™ Rocket™ (High Speed) Columns

○

○

○

SAX

PART NO.

*Requires Guard Holder

○

100Å 5µm 150 x 4.6mm 5µm 250 x 4.6mm

QTY

All-Guard™ Cartridges (Requires Guard Holder) C18 100Å 5µm 7.5 x 4.6mm 3 32606 300Å 5µm 7.5 x 4.6mm 3 32610 EPS C18 100Å 5µm 7.5 x 4.6mm 3 32607 300Å 5µm 7.5 x 4.6mm 3 32611 C8 100Å 5µm 7.5 x 4.6mm 3 32612 EPS C8 100Å 5µm 7.5 x 4.6mm 3 32614 300Å 5µm 7.5 x 4.6mm 3 32616 C4 300Å 5µm 7.5 x 4.6mm 3 32617 EPS C4 300Å 5µm 7.5 x 4.6mm 3 32618 Phenyl 100Å 5µm 7.5 x 4.6mm 3 32619 Cyano 100Å 5µm 7.5 x 4.6mm 3 32620 Amino 100Å 5µm 7.5 x 4.6mm 3 32621 SAX – 5µm 7.5 x 4.6mm 3 32787 Silica 100Å 5µm 7.5 x 4.6mm 3 32622

All-Guard™ Cartridge Holder

Cyano

Spherical Silica 1.5-, 3-, 5-, 10-µm 0.78cc/g 100m2/g

Platinum™ Guard Columns* DESCRIPTION

EPS C18

300Å PACKINGS

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i

388

© Copyright 2003 Alltech Associates, Inc.

For more information about Rocket™ Columns, see pages 356-357.

Contact your Alltech office or distributor for current or local prices.

Alltech Platinum™ 1

Anti-Inflammatories

Anticonvulsants

3

8946

1

8156

2

3

4

1. 2. 3. 4.

2 1. Naproxen 2. Acemethacin 3. Indomethacin 4. p-Xylene

4

4

6

8

0

10 Min.

Column: Standard Platinum™ C18, 5µm, 150 x 4.6mm Mobile Phase: Methanol:Acetonitrile:0.02M KH2PO4 pH4.5. (50:10:40) Flowrate: 1.0mL/min Detector: UV at 254nm

4

1

3 Min.

2

Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ Mobile Phase: 0.025M Ammonium Acetate, pH 5.5:Acetonitrile (70:30) Flowrate: 3.0mL/min Detector: Alltech ELSD

Sedatives/Hypnotics

Antibacterials 8160

8711

5 1. Barbital 2. Phenobarbital 3. Butalbital 4. Oxazepam 5. Triazolam

2 1 3

5 2

4

Column: Mobile Phase: Flowrate: Detector:

6

10 Min.

Platinum™ EPS C18, 5µm, 150 x 4.6mm 0.025M KH2PO4 pH3:Acetonitrile (90:10) 1.0mL/min UV at 230nm

Barbiturates 8691

1. Chlordiazepoxide degradant 2. Nitrazepam 3. Norchlordiazepoxide

8684

4. Nordiazepam 5. Chlordiazepoxide 6. Chlordiazepoxide degradant 7. Diazepam

3

8

3

5 4 2

1

4 5 6

○

1

7

1. Barbital 2. Butalbarbital 3. Aprobarbital 4. Talbutal 5. Butabarbital 6. Phenobarbital 7. Amobarbital 8. Mephobarbital

hplc hplc

Benzodiazepines and Metabolites

2

8

○

Column: Standard Platinum™ C18, 5µm, 150 x 4.6mm Mobile Phase: Methanol:Acetonitrile:0.05M NH4H2PO4, pH 4.5, (50:10:40) Flowrate: 1.0mL/min Detector: UV at 230nm

2

○

0

6 Min.

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4

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2

Sulfanilimide Cefaclor Cefatrizine Thiamphenicol Cefotaxime

7

12 Min.

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8

○

4

Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ Mobile Phase: 0.010M Sodium Acetate, pH4.0:Acetonitrile (75:25) Flowrate: 3.0mL/min Detector: UV at 230nm

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0

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ Mobile Phase: 0.05M Ammonium Acetate, pH5.5:Acetonitrile (65:35) Flowrate: 3.0mL/min Detector: UV at 254nm

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6 Min.

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4

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2

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0

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6

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0

4

3

1

1. 2. 3. 4. 5.

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2

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0

Ethotoin Mephenytoin 5,5-Diphenylhydantoin Carbamazepine

389

Alltech Platinum™ Peptides (≤ 6 Amino Acid Residues) 1. 2. 3. 4. 5. 6.

Val-Gly-Ser-Glu L-Phenylalanylglycine L-Phenylalanylglycylglycine 6 Val-Gly-Val-Ala-Pro-Gly L-Phenylalanyl-L-leucine L-Tryptophyl-L-phenylalanine 2 3 4

1

Peptides (> 5 Amino Acid Residues) 8683

8723

1

5

7 8

3 2 4 6 0

5

8

4

9

12

16

10

20

24 Min.

Platinum™ EPS C18, 5µm, 150 x 4.6mm Mobile Phase: A: 0.15% v/v TFA in Water B: 0.13% v/v TFA in Acetonitrile:Water (95:5) Gradient: Time: 0 2 30 %B 15 15 55 Flowrate: 3.0mL/min Detector: UV at 216nm Column:

0 2 4 6 8 Column: Platinum™ EPS 100Å C18, 3µm, 53 x 7mm Rocket™ Mobile Phase: A: 0.15% TFA in Water B: 0.13% TFA in 95:5 Acetonitrile:Water Gradient: Time: 0 1 10 B%: 10 10 50 Flowrate: 3.0mL/min Detector: UV at 216nm

Nucleosides

Thyroid Chemicals 1. DL-Tyrosine 8282 2. 3-Iodo-Tyrosine 3. 3,5- Diiodo-D-Tyrosine 4. DL-Thyroxine 5. 3,3,5-Triiodo-L-Thyronine 5

8245

4

5

3

2 1

0

5

10

15

1. 2. 3. 4. 5.

2 1 3

Cytidine Uridine Xanthine Guanosine Adenosine

4

0

20 Min.

Column: Platinum™ EPS C18, 5µm, 250 x 4.6mm Mobile Phase: A: 0.03M KH2PO4 pH3.2 B: Acetonitrile Gradient: Time: 0 2 20 %B 5 5 30 Flowrate: 0.7mL/min Detector: UV at 260nm

hplc hplc

1. Peptide T 2. (Arg-8)Vasotocin 3. (Lys-8)Vasopressin 4. (Arg-8)Vasopressin 5. Methionine Enkephalin 6. Oxytocin 7. Leucine Enkephalin 8. Physalaemin 9. Bombesin 10. Angiotensin I

2

4

Column: Mobile Phase: Flowrate: Detector:

6

8

10

12 Min.

Platinum™ EPS C18, 5µm, 150 x 4.6mm 0.05M KH2PO4, pH 3.2:Acetonitrile (65:35) 1.0mL/min UV at 254nm

Monophosphate Nucleotides

Methylxanthines

3 8296

○

2

8384

4

5

1. 2. 3. 4. 5.

Theobromine Theophylline 8-Chlorotheophylline Caffeine 7-(2-chloroethyl)theophylline

○

○

○

○

3 1 2

1. Cytidine-5'-monophosphate 2. Uridine-5'-monophosphate 3. Guanosine-5'-monophosphate 4. Adenosine-5'-monophosphate

4

0

2

4

6

8

10 12 14 16 Min.

Column: Mobile Phase: Flowrate: Detector:

Platinum™ EPS C18, 5µm, 150 x 4.6mm Methanol:0.05M Ammonium Acetate pH5.5 (5:95) 0.7mL/min UV at 254nm

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1

390

© Copyright 2003 Alltech Associates, Inc.

0 2 Column:

4 6 8 10 Min. Platinum EPS C18, 100Å, 5µm, 150 x 4.6mm (Part No. 32214) Mobile Phase: Water:Acetonitrile:Methanol: Glacial Acetic Acid (60:10:30:1) Flowrate: 1.0mL/min Detector: UV at 254nm Contact your Alltech office or distributor for current or local prices.

Alltech Platinum™ Nitroaromatic and Nitroamine Explosives

Metal Chelators 2

1. 2. 6 3. 4. 5. 6.

4

3

1

0

2

5

2

4

6

Column: Column Temp: Mobile Phase: Flowrate: Detector:

8

10

7

12

8279 7. TNT HMX 8. 4-A-DNT RDX 9. 2,6DNT TNB 10. 2,4DNT DNB Tetryl 11. 2-NT 12. 3-NT NB 13. 4-NT 13 10 11 12 9 8

14

16

18

20

22 Min.

8125

1 1. Pyridine 2. Dipyridyl

0

2

1

Column: Mobile Phase: Flowrate: Detector:

Standard Platinum™ C18, 5µm, 250 x 4.6mm 30°C Methanol:Water (50:50) 0.7 mL/min. UV at 254nm

3 Min.

Platinum C18, 100 x 4.6mm Acetonitrile:0.05M KH2PO4 pH7.0 (50:50) 2.0mL/min UV at 254nm

Semipolar & Highly Hydrophilic Waste Water Contaminants 1. 4-Amino-5-hydroxy-2,7-naphthalene-disulfonic Acid 2. Hydroquinone 3. Benzylamine 4. 3'-Aminoacetophenone 5. Phenol 6. 4-Hydroxyphenylacetic Acid 7. 1-Naphthalene Sulfonic Acid 8. 2,4-Dihydroxybenzoic Acid 9. 1-Naphthalene Sulfonic Acid Degradant 10. 4-Hydroxy-3-methoxybenzoic Acid 11. Phenylacetic Acid 12. m-Cresol 13. 4-Hydroxycinnamic Acid 14. trans-Cinnamaldehyde 15. trans-Cinnamaldehyde Degradant 16. trans-Cinnamaldehyde Degradant 17. 2-Naphthalenethiol Degradant 18. 2-Naphthalenethiol 19. 2-Naphthalenethiol Degradant

11 12

9

10

14 13

20

16 15 17

30

18 19

40

50 Min.

Platinum™ EPS C18, 5µm, 250 x 4.6mm A: 0.03M NaH2PO4 pH2.5 B: Acetonitrile:Methanol:Water (40:50:10) Time: 0 3 13 20 25 30 40 45 55 %B: 5 5 10 10 20 40 75 80 80 1.0mL/min UV at 210nm

Column: Mobile Phase: Gradient: Flowrate: Detector:

Organochlorine Pesticides 1

8671

○

3

7

6

9

12 Mi

○

3

○

Standard Platinum™ C18, 5µm, 150 x 4.6mm Methanol:0.025M K2HPO4, pH7 (75:25) 1.0mL/min UV at 220nm

○

Column: Mobile Phase: Flowrate: Detector:

○

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0

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2 3 4 5 Min. Platinum™ EPS C18, 1.5µm, 33 x 7mm Rocket™ 0.025M KH2PO4 pH3:Acetonitrile (65:35) 2.0mL/min UV at 254nm

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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0 1 Column: Mobile Phase: Flowrate: Detector:

○

○

○

○

3

2

○

6 4 5

4

1. Methoxychlor 2. Dieldrin 3. Heptachlor 4. 4,4-DDT

○

2

8437

1. Simazine 2. Simetryn 3. Prometon 4. Atrazine 5. Ametryn 6. Prometryn 7. Terbutryn

1

hplc hplc

Triazine Herbicides

○

0

6

7

○

23

4

○

10

5

1

○

8

○

8447

391

Alltech Platinum™ Strong Bases

Positional Isomers of Nitroaniline

2

8152

2 3

8900

3

1. 2. 3. 4.

4

1. o-Nitroaniline 2. p-Nitroaniline 3. m-Nitroaniline

Procaine Mepivacaine Tetracaine Dibucaine

1 1

0

2

4

6

8

10 Min.

0

Platinum™ EPS C18, 5µm, 250 x 4.6mm 50:50 Acetonitrile:50mM KH2PO4, pH3 1.0mL/min UV at 220nm

Columns: Mobile Phase: Flowrate: Detector:

1

2

3

Column: Mobile Phase: Flowrate: Detector:

4

5 Min.

Standard Platinum™ C18, 5µm, 150 x 4.6mm Acetonitrile:0.05M KH2PO4 pH3.15, (50:50) 1.0mL/min UV at 254nm

Catechins in Green Tea

Water Soluble Vitamins 7

1 1. 2. 3. 4. 5. 6. 7. 8.

1 3 2

5 4 6 7

Gallic Acid Epigallocatechin Catechin Epicatechin Epigallocatechin Gallate Caffeine Gallocatechin Epicatechin Gallate

5

2

4

6

8

Organic Acids 8244

3

7

1 ○

○

1. Uracil 2. Benzamide 3. Benzyl Alcohol 4. Phenol 5. Methylbenzoate 6. Acetophenone 7. N,N-diethyl-m-toluamide 8. Toluene 9. Benzophenone 10. Biphenyl

○ ○ ○ ○ ○

○

6 5 8

2

4

1. Tartaric Acid 2. Malic Acid 3. Lactic Acid 4. Diglycolic Acid 5. Maleic Acid 6. Succinic Acid 7. Fumaric Acid

6

0

1

2

3

4 Min.

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1

10 12 14 16 Min.

5 8982

7 9 10

3 4

Ascorbic Acid Nicotinic Acid Pyridoxamine Niacinamide Folic Acid Riboflavin Vitamin B12

Column: Standard Platinum™ C18, 5µm, 150 x 4.6mm Mobile Phase: A: 0.1M NH4H2PO4 pH4.5 B: Acetonitrile Gradient: Time: 0 4 15 18 B%: 5 5 40 40 Flowrate: 1.0mL/min Detector: UV at 254nm

Monoaromatics 2

6

1. 2. 3. 4. 5. 6. 7.

8

2 3 4 5 Min. Platinum™ EPS C18, 1.5µm, 33 x 7mm Rocket™ 0.05% v/v TFA in 87:13 Water:Acetonitrile 2.5mL/min. UV at 210nm

hplc hplc

4

3

0 0 1 Column: Mobile Phase: Flowrate: Detector:

8154

2

8825

392

Column: Mobile Phase: Flowrate: Detector:

Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ 0.05M KH2PO4, pH 3:Methanol (50:50) 2.3mL/min UV at 254nm

© Copyright 2003 Alltech Associates, Inc.

0 2 4 6 8 10 Min. Column: Platinum™ EPS C8, 5µm, 150 x 4.6mm Mobile Phase: 0.025M KH2PO4, pH 2.5: Methanol (97:3) Flowrate: 0.7mL/min Detector: UV at 220nm Contact your Alltech office or distributor for current or local prices.

Alltech Prevail A polar-embedded amide phase on a pure silica particle produces very symmetrical peaks for basic analytes in neutral pH mobile phases. Refer to page 399 for information about Prevail™ Amide Columns.

Alltech Prevail™ HPLC Columns • A General Purpose Column for the Widest Range of Mobile Phases • Stable in 100% Aqueous Mobile Phases • Excellent Retention and Reproducibility of Highly-Polar Analytes • Strong Retention of Hydrophobic Analytes in 100% Organic Mobile Phases

Prevail™ OA Columns make fast work of organic acid determinations. The silica-based reversed-phase columns are quality controlled at ambient temperature with a mixture of 11 organic acids in a 6 minute separation. Prevail OA Columns are described on page 402. Separate carbohydrates and oligosaccharides on a Prevail™ Carbohydrate ES Column—get the performance of a silica-based amino column and the chemical stability of a polymer-based column. Replace your amino carbohydrate column with a Prevail™ Carbohydrate ES Column and see significant improvements in retention time reproducibility, column life, and detector baselines (and sensitivity), all without changing your mobile phase or method. Find Prevail™ Carbohydrate ES Columns on pages 400-401.

Prevail™ Columns were designed to conquer those difficult HPLC separations that have long challenged base6138 deactivated silica columns. The unique C18 bonding chemistry is stable in highly aqueous mobile phases, and highly-polar analytes are well retained with excellent reproducibility. The same C18 columns effectively retain and resolve highly-nonpolar, hydrophobic analytes with 100% organic mobile phases. The Prevail™ C18 Columns are so durable that a single column can routinely be used with and switched between highly aqueous and highly organic mobile phases.

Prevail™ Columns are available in a wide selection of column formats, from 22mm prep columns to small volume columns for high throughput and high sensitivity applications with MS detectors. Ordering information for the more popular columns is presented on pages 394 and 395.

Switch Between 100% Aqueous and 100% Organic Mobile Phases On the Same Column

6

8

10 12 14 Min.

Prevail™ C18, 5µm, 150 x 4.6mm Mobile Phase: 25mM KH2PO4 pH 3.0: CH3CN (97:3) Flowrate: 1.0mL/min Detector: UV at 212nm

2

4

6

8

10

12

14 Min.

Prevail™ C18, 5µm, 150 x 4.6mm Mobile Phase: CH3CN:CH3OH (75:25) Flowrate: 1.5mL/min Detector: UV at 220nm

Column:

6

1

0

2

4

6

8

10 12 14 Min.

Prevail™ C18, 5µm, 150 x 4.6mm Mobile Phase: 25mM KH2PO4 pH3.0: CH3CN (97:3) Flowrate: 1.0mL/min Detector: UV at 212nm Column:

Figure 2

Figure 3

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© Copyright 2003 Alltech Associates, Inc.

○

Contact your Alltech office or distributor for current or local prices.

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○

Prevail™ Columns have highly stable bonded phases that let you use one column for multiple mobile phase conditions. Even switching between extremes such as near 100% aqueous (Figure 1) to 100% organic conditions (Figure 2), and back to near 100% aqueous (Figure 3) on a routine basis is possible, provided that mobile phases are miscible.

○

○

○

Figure 1

○

○

Column:

0

5

○

4

7

2 3

4

○

2

2

hplc hplc

0

wash with intermediate

5 1

6

1

3

○

wash with intermediate

5

9466 1. Thiamine 2. Ascorbic Acid 3. Nicotinic Acid 4. Pyridoxine 5. Niacinamide 6. Pantothenic Acid

○

4

9390

1. Vitamin A 5. Vitamin D2 2. δ-Tocopherol 6. Vitamin D3 3. γ-Tocopherol 7. Vitamin K 4. α-Tocopherol (Vitamin E) 6 4

○

9465

1. Thiamine 2. Ascorbic Acid 3 3. Nicotinic Acid 4. Pyridoxine 5. Niacinamide 6. Pantothenic Acid 2

Water Soluble Vitamins (100% Aqueous)

Fat Soluble Vitamins and Tocopherols (100% Organic)

Water Soluble Vitamins (100% Aqueous)

393

Alltech Prevail™ Exceptional Stability - Long Column Life Even Under Extreme Conditions.

Prevail™ Analytical Columns Prevail™ Columns PACKING

Stability Study

9382

LENGTH X ID

C18

3µm 3µm 3µm 3µm 5µm 5µm 5µm

150 x 2.1mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm

99200 43829 99202 99204 99206 99208 99210

99201 43901 99203 99205 99207 99209 99211

C8

3µm 3µm 3µm 3µm 5µm 5µm 5µm

150 x 2.1mm 50 x 4.6mm 100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm

99212 43922 99214 99216 99218 99224 99229

99213 43923 99215 99217 99219 99228 99230

Phenyl

3µm 3µm 5µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm

99233 99235 99237 99239 99241

99234 99236 99238 99240 99242

Cyano

3µm 3µm 3µm 5µm 5µm 5µm

150 x 2.1mm 100 x 4.6mm 150 x 4.6mm 150 x 2.1mm 150 x 4.6mm 250 x 4.6mm

99243 99245 99247 99249 99251 99253

99244 99246 99248 99250 99252 99254

Amino

3µm 5µm 5µm

150 x 2.1mm 150 x 4.6mm 250 x 4.6mm

99255 99263 99265

99256 99264 99266

Silica

5µm 5µm

150 x 4.6mm 250 x 4.6mm

99275 99277

99276 99278

OA

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

88650 88655 88640 88645

88750 88755 88740 88745

Amide

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

88670 88675 88660 88665

88690 88694 88680 88685

Carbohydrate ES 5µm 5µm

150 x 4.6mm 250 x 4.6mm

— —

35102 35101

9383

Initial Separation

After 500 Hours at pH 1.0 and 60° C

1. Uracil 2. Phenol 3. N, N-Diethylm-toluamide 2 4. Toluene

2

4

4 3

3 1

1

0 1 2 3 4 5 6 7 Min.

0 1 2 3 4 5 6 7 Min.

Prevail™ C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: CH3CN:H2O (58:42) Flowrate: 1.0mL/min Detector: UV at 254nm Column:

A Prevail™ C18 Column was washed for 500 hours at 60° C with an acetonitrile:water mobile phase adjusted to pH 1.0 with sulfuric acid. The column showed no chromatographic change.

Tricyclic Antidepressants 9408 Run Time and Mobile Phase Consumption Reduced by 50%

1 2

1

hplc hplc

2

9409

1. Doxepin 2. Nortriptyline 3. Trimipramine

3 3 pH 4.2

PRICE

0 2

4

6 8 10 12 14 16 18 20 22 24 Min.

Prevail™ C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: 25mM KH2PO4:CH3CN (65:35) Flowrate: 1.0mL/min Detector: UV at 210nm Column:

Take advantage of Prevail™’s stability by analyzing amines at lower pH to reduce the analysis time. The analysis done at pH 1.0 is completed in half the time.

Prevail™ All-Guard™ Cartridges* PACKING

PART SIZE

LENGTH X ID

QTY

PART NO.

7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm

3 3 3 3 3 3 3 3

99286 99287 99288 99289 99290 99291 96429 96443

All-Guard™ Guard Cartridge Holder

1

80101

All-Guard™ Cartridges C18 5µm C8 5µm Phenyl 5µm Cyano 5µm Amino 5µm Silica 5µm OA 5µm Amide 5µm

PRICE

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pH 1.0 w/H2SO4

INDUSTRY STANDARD WATERS PART NO. PART NO.

PARTICLE SIZE

394

i

For Prevail™ Specialty Phases see pages 399-402.

© Copyright 2003 Alltech Associates, Inc.

*Requires Guard Holder

Contact your Alltech office or distributor for current or local prices.

Alltech Prevail™ Prevail™ LC/MS Columns

Fast Gradient 9460

45

1 6114

7

Prevail™ LC/MS Columns PACKING

3 LENGTH X ID

INDUSTRY STANDARD PART NO.

3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm

10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm

43861 43827 43818 43871 99200 43878 43804 43831 43843

3µm 3µm 3µm 3µm 3µm 3µm 3µm

10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm

43841 43826 43868 43805 99267 43858 43816

3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm

10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm

43916 43915 43914 43913 43912 43918 43917 43928 43919

3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm 3µm

10 x 2.1mm 20 x 2.1mm 50 x 2.1mm 100 x 2.1mm 150 x 2.1mm 10 x 4.6mm 20 x 4.6mm 100 x 1.0mm 150 x 1.0mm

43873 43885 43819 43872 99231 43887 43815 43893 43859

C18 Expedite™ Expedite™

Expedite™ Expedite™

Silica Expedite™ Expedite™

Expedite™ Expedite™ Amide Expedite™ Expedite™

Expedite™ Expedite™

Phenyl Expedite™ Expedite™

6 8 9 2

0

1

2

3 Min. ™

Prevail C18, 3µm, 20 x 2.1mm, Expedite™ (Part No. 43827) Mobile Phase: A: 0.1% TFA in H2O B: 0.08% TFA in CH3CN Gradient: Time: 0 2 3 %B: 0 100 100 Flowrate: 1.0mL/min Detector: UV at 254nm

Column:

Prevail™ High Speed Rocket™ Columns 3806

Prevail™ Rocket™ Columns PACKING

PART SIZE

LENGTH X ID

PART NO.

C18

3µm 3µm

53 x 7mm 33 x 7mm

99279 99280

Phenyl

3µm 3µm

53 x 7mm 33 x 7mm

99281 99282

Silica

3µm 3µm

53 x 7mm 33 x 7mm

99283 99284

OA

3µm 3µm

53 x 7mm 33 x 7mm

50755 99292

Amide

3µm 3µm

53 x 7mm 33 x 7mm

50775 99298

Carbohydrate ES 5µm 5µm

53 x 7mm 100 x 7mm

35104 35103

PRICE

○

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Expedite™ Expedite™

PRICE

Uracil Theophylline Acetophenone Propiophenone Butyrophenone Benzophenone Valerophenone Heptanophenone Octanophenone

hplc hplc

PARTICLE SIZE

1. 2. 3. 4. 5. 6. 7. 8. 9.

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Prevail™ Preparative Columns

LENGTH X ID

PART NO.

5µm 5µm 5µm 5µm

150 x 10mm 250 x 10mm 150 x 22mm 250 x 22mm

99293 99294 99296 99297

PRICE

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PART SIZE

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970211

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Prep-Guard Prevail™ C18 10µm 33 x 7mm

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C18

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PACKING

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Prevail™ Preparative Columns

395

Alltech Prevail™ Nucleosides

Nucleic Acid Bases

1 9410

9411

2 5

1

1. 2. 3. 4. 5.

4 3

0

2

4

6

Cytidine Uridine Xanthine Guanosine Adenosine

2

1. 2. 3. 4. 5.

5 4

8 Min.

0

Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Acetonitrile:25mM KH2PO4, pH 3.0, (4:96) 1mL/min UV at 254

Column: Mobile Phase: Flowrate: Detector:

3

5 Amino Acids, Underivatized

2

4

Column: Mobile Phase: Flowrate: Detector:

6

8 Min.

Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Acetonitrile:25mM KH2PO4, pH 3.0, (2:98) 1mL/min UV at 254

Water Soluble Vitamins from Multi-Vitamin Tablet 9449

9456

3

1. 2. 3. 4. 5. 6. 7.

7 1 23 4 6

Cytosine Uracil Guanine Adenine Thymine

Glycine Serine Aspartic Acid Glutamine Alanine Glutamic Acid Lysine

1

2

6

1. 2. 3. 4. 5. 6. 7.

5

Thiamine Ascorbic Acid Unknown Fumaric acid Pyridoxine Niacinamide Pantothenic Acid

4 7

hplc hplc

0 1 2 3 4 5 6 7 8 9 10 Min.

Column: Mobile Phase: Flowrate: Detector:

Prevail® C18, 5µm, 250 x 4.6mm (Part No. 99210) 5mM Heptafluorobutyric Acid, pH1.0 w/0.7% TFA 1mL/min Alltech ELSD

0 2 4 Column: Mobile Phase: Flowrate: Detector:

6 8 10 12 14 Min. Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) 25mM KH2PO4, pH3.0: Acetonitrile (97:3) 1mL/min UV at 212

Acids, Bases, & Neutrals Mix

Anticonvulsants 3

9406

9394

○

1

○

○

1. 2. 3. 4. 5. 6. 7.

4

○

○

○

○

2 3

○

5

7

4 1

2

1. 2. 3. 4.

Ethotoin Mephenytoin Carbamazepine Diazepam

0

2

4

6

8

10

12

14

16 Min.

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6

Uracil Phenol 4-Phenylbutyric acid N,N-Diethyl-m-toluamide Propylbenzene Quinizarin Butylbenzene

396

Column: Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: (65:35) Acetonitrile: 25 mM KH2PO4 pH 2.5 Flowrate: 1mL/min Detector: UV at 254 © Copyright 2003 Alltech Associates, Inc.

0 Column:

2

4 6 8 Min. Prevail™ Phenyl, 5µm, 150 x 4.6mm (Part No. 99239) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 15 %B: 25 60 Flowrate: 1.5mL/min Detector: UV atoffice 254 or distributor for current or local prices. Contact your Alltech

Alltech Prevail™ Hydrocortisone Acetate Cream

Steroids

1

3 9463

9392

2 3

1. Methyl Paraben 2. Propyl Paraben 3. Hydrocortisone Acetate

1

1. Hydrocortisone 2. Testosterone 3. Progesterone

2

0 0 1 2 3 4 5 Min. Column: Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: (90:10) Acetonitrile:Water Flowrate: 1mL/min Detector: UV at 254

1 Min.

Prevail® C18, 3µm, 53 x 7mm Rocket™ (Part No. 99279) Mobile Phase: (90:10) Methanol : Water Flowrate: 4.5mL/min Detector: UV at 254 Column:

Polyphenolic Flavonoids

Long Chain Fatty Acids 9397

4

3

1. 2. 3. 4. 5. 6.

1 2

4

9459

3

5

6

Myricetin Quercetin Naringenin Hesperetin Apigenin Kaempferol

5

1. 2. 3. 4. 5.

Capric Acid Lauric Acid Myristic Acid Palmitic Acid Stearic Acid

2 1

6 8 10 12 14 16 18 20 22 24 26 Min.

2 3 4 Min. Prevail® C18, 3µm, 53 x 7mm (Part No. 99279) (75:25) Acetonitrile:Methanol 3.5mL/min Alltech ELSD

Polyaromatic Hydrocarbons

14

16

13 8 9

○ ○ ○

15

2

○ ○

17

3 10

Contact your Alltech office or distributor for current or local prices.

Column: Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 4 15 %B: 72 72 100 Flowrate: 1.5mL/min Detector: UV at 254 © Copyright 2003 Alltech Associates, Inc.

○

16 Min.

○

14

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12

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10

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8

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6

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4

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2

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0

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○

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1

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12

9400

○

7

5

10. 1,2-Dibenzanthracene 11. Chrysene 12. Benzo(b)fluoranthene 13. Benzo(k)fluoranthene 14. Benzo(a)perylene 15. 1,2,5,6-Dibenzanthracene 16. 1,2-Dibenzoperylene 17. Indeno[1,2,3,c,d]pyrene

○

11

Benzene Naphthalene Acenaphthylene Fluorene Acenaphthene Phenanthrene Anthracene Fluoranthene Pyrene

○

4 6

1. 2. 3. 4. 5. 6. 7. 8. 9.

○

0 1 Column: Mobile Phase: Flowrate: Detector:

hplc hplc

Column: Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: 25mM KH2PO4, pH2.5 B: Acetonitrile Gradient: Time: 0 15 25 %B: 20 40 40 Flowrate: 1.0mL/min Detector: UV at 280

○

0 2 4

397

Alltech Prevail™ Vitamin E Soft Gel Tablet

Fat Soluble Vitamins and Tocopherols

3 9458

2

9390

4

1. ∆-Tocopherol 2. γ-Tocopherol 3. α-Tocopherol

1. 2. 3. 4. 5. 6. 7.

6

5 1 2 3

Vitamin A ∆-Tocopherol γ-Tocopherol α-Tocopherol (Vitamin E) Vitamin D2 Vitamin D3 Vitamin K

7

1

0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Min. Column: Mobile Phase: Flowrate: Detector:

Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Acetonitrile:Methanol (75:25) 1.5mL/min Alltech ELSD

2

4

6

12

14 Min.

Antibacterials

5

9446

1. 2. 3. 4. 5.

3

10

Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Acetonitrile:Methanol (75:25) 1.5mL/min UV at 220

Column: Mobile Phase: Flowrate: Detector:

Beverage Additives

2

8

9396

3

Ascorbic Acid Saccharin Benzoic Acid Aspartame Vanillin

6 2

5

11

9

4

10

7 8

1

1

4

hplc hplc

0

1

2

0 2 4 6 8 10 12 14 16 18 20 22 Min.

3 Min.

Column: Prevail™ C18, 3µm, 50 x 2.1mm (Part No.43818) Mobile Phase: Acetonitrile:0.05M Ammonium Acetate pH 5.4, (20:80) Flowrate: 0.3 ml/min Detector: UV at 230 nm

1. Neomycin 2. Amoxicillin 3. Vancomycin 4. Cefatrizine 5. Metampacillin 6. Cefotaxime 7. Unknown 8. Bacitracin 9. Pipericillin 10. Bacampacillin 11. Oxacillin

Column: Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: 25mM KH2PO4, pH3.0 B: Acetonitrile Gradient: Time: 0 15 22 %B: 10 40 40 Flowrate: 1.0mL/min Detector: UV at 210

Antihypertensives

Peptides

3 9395

9399

○

○

3 4

2

○

1

○

2

7 6

1 8

GLY-TYR VAL-TYR-VAL Methionine-Enkephalin Physalemin Leucine-Enkephalin Oxytocin Angiotensin II Substance P

0 Column: Mobile Phase: Flowrate: Detector:

1 2 Min. Prevail® C18, 3µm, 53 x 7mm Part No. (99279) 25mM KH2PO4, pH3.0:Acetonitrile, (70:30) 4.0mL/min UV at 220

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1. Metoprolol 2. Oxprenolol 3. Propranolol

5

1. 2. 3. 4. 5. 6. 7. 8.

398

© Copyright 2003 Alltech Associates, Inc.

0 2 4 6 8 10 12 14 Min. Column: Prevail® C18, 5µm, 150 x 4.6mm (Part No. 99208) Mobile Phase: A: 0.15%TFA in Water B: 0.13%TFA in Acetonitrile:Water (95:5) Gradient: Time: 0 15 %B: 20 55 Flowrate: 1.0mL/min Detector: UV at 220 Contact your Alltech office or distributor for current or local prices.

Alltech Prevail™ Amide Column Bases on Prevail™ Amide at pH 7

Improved Peak Shape with Prevail™ Amide

10181

• Polar Embedded Group Improves Peak Shape of Polar Samples • Packing Media Provides Unique Selectivity • Bonded Phase does not Collapse Under 100% Aqueous Mobile Phase A Polar Embedded Group inserted into the hydrophobic chain of the Prevail™ Amide phase minimizes interaction of polar samples with silanols, providing symmetrical peaks for a wide variety of applications. The spatial location of the polar amide group within the reversed phase chain allows it to strongly interact with silanols so that your basic samples do not. This is especially important at neutral pH where the peak shape of many polar organic compounds is compromised. The presence of the amide group alters the selectivity of the reversed-phase packing allowing separations often hard to achieve on conventional reversed-phase columns. Most C18 phases are not wettable with 100% aqueous mobile phases and collapse when challenged with an all-aqueous solvent. The amide group keeps the bonded phase from collapsing under 100% aqueous conditions, allowing the analyst to use these mobile phases to more fully resolve polar analytes.

1

2

1. Diphenhydramine 2. Amitriptyline

0

1

2

3

4

5

6

7 Min.

Prevail™ Amide, 5µm, 150 x 4.6mm (Part No. 88660) Mobile Phase: (30:70) 25mM Potassium Phosphate, pH 7.0:Methanol Flowrate: 1.0mL/min Detector: UV at 254 Column:

Analgesics

1

9385

Prevail™ Amide HPLC Columns WATERS INSTRUMENT PART NO.

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

88670 88675 88660 88665

88690 88694 88680 88685

PRICE

1. Aspirin 2. Acetaminophen 3. Naproxen 4. Fenprofen

2

Amide 3

Prevail™ Amide Rocket™ Columns PHASE

PARTICLE SIZE

0

LENGTH X ID

PART NO.

2

4

6

4

8 10 12 14 Min.

PRICE

Prevail™ Amide, 5µm, 150 x 4.6mm (Part No. 88660) 20mM K2HPO4, pH7.0:CH3CN (75:25) 1.0mL/min UV at 210nm

Column:

Amide 3µm 3µm

53 x 7mm 33 x 7mm

50775 99298

Mobile Phase: Flowrate: Detector:

PHASE

Amide

PARTICLE SIZE

LENGTH X ID

QTY

PART NO.

5µm

7.5 x 4.6mm

3

96429

Each

80101

All-Guard Cartridge Holder

Anilines

PRICE

9424

hplc hplc

Prevail™ Amide All-Guard Cartridges*

1 1. Aniline 2. Dimethylaniline 3. Diethylaniline

○

*Requires Guard Holder

○

3µm 3µm 5µm 5µm

LENGTH X ID

INDUSTRY STANDARD PART NO.

○

PHASE

PARTICLE SIZE

○

○

2

1

2

3

4

5

6

7

8

9

10 Min.

○ ○ ○ ○ ○ ○

Contact your Alltech office or distributor for current or local prices.

○

Mobile Phase: Flowrate: Detector:

Prevail™ Amide, 3µm, 150 x 4.6mm (Part No. 88675) 20mM K2HPO4 pH7.0:CH3CN (50:50) 1.0mL/min UV at 210nm © Copyright 2003 Alltech Associates, Inc.

○

Column:

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0

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3

399

Alltech Prevail™ Carbohydrate ES Column Prevail™ Carbohydrate ES Columns – Exceptional Performance

Exceptionally Quiet Gradient Baselines

• Unprecedented Ruggedness • Allows Gradient Separations with ELSD • Delivers Optimum Resolution and Peak Shapes • Outperforms Other Carbohydrate Columns used with the ELSD or RI and UV Detectors

The Prevail™ Carbohydrate ES Column delivers excellent isocratic separations, but the column’s real power is released when used with the ELSD, a detector blind to the mobile phase, and a solvent gradient. Gradients with Prevail™ Carbohydrate ES Columns show flat, quiet baselines, free from noise and drift. Not only are gradients possible, they’re recommended because they offer powerful selectivity, reduced run times, and enhanced detector sensitivity as peak efficiencies are maximized. Finally, solvents may be chosen for their chromatography advantages instead of their spectral properties (Figure 2).

6186

The Alltech Prevail™ Carbohydrate ES Column is free from the problems that plague other carbohydrate columns. It offers unprecedented ruggedness and baseline stability with all detectors, including the ELSD. The polymeric Prevail™ Column shows none of the chemical degradation that limits the lifetime of silica-based amino columns (Figure 1). During product development, a single 250 x 4.6mm Prevail™ Carbohydrate ES Column was used daily for all appropriate supporting chromatography for twelve months with no discernable degradation!

Exceptional Resolution and Peak Shape Prevail™ Carbohydrate ES Columns are versatile enough for monoand oligosaccharides and sugar alcohols. They quickly resolve fructose, glucose, lactose, maltose, and oligosaccharides with high peak efficiencies. Further, Prevail™ Carbohydrate ES Columns produce single peaks for reducing sugars like glucose at ambient temperatures, eliminating the need for column heating. If you cannot step up to the benefits of Alltech ELSD detection, you can still enjoy the advantages of the Prevail™ Carbohydrate ES Columns with your existing carbohydrate analysis system.

Enhanced Detector Sensitivity Through Flat Gradient Baselines 9354

12 9379

1. Dextrose 2. Maltose 3. Maltotriose 4. Maltotetraose 5. Maltopentaose 6. Maltohexaose 7. Maltoheptaose 8. Maltooctaose

9486

13

1

14 15

2 3

18 19 20 21 22 23 24 Min. 4 5 1

2 3

6 78

4 5 6

11 13 10 9 12

7

Prevail™ Carbohydrate ES Column (Part No. 35101)

Column: 250 x 4.6mm as identified Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 15 %B: 35 50 Flowrate: 1.0mL/min Detector: ELSD 2000

Silica Based Amino Column

hplc hplc

0

5

10

15

20

25 Min.

Figure 1 - When used with the ELSD 2000 Detector, the Prevail™ Carbohydrate ES Column permits the effective use of gradients in carbohydrate separations, reducing run times and enhancing detector sensitivity. By comparison, silica-based amino columns produce noisy, shifting baselines.

Reduced Run Times Using Alternative Solvents

9350

○

○

○

○

○

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○

○

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○

Unmodified Mobile Phase Prevail™ Carbohydrate ES, 250 x 4.6mm Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 50 %B: 20 35 Flowrate: 1.0mL/min 12 3 Column Temp: Ambient 4 56 Detector: ELSD 2000 7 Column:

1. Fructose 2. Glucose 3. Sucrose

4. Maltose 5. Maltotriose 6. Maltotetraose 7. Maltopentaose 1 2 3 4

5 67

9360

Modified Mobile Phase Prevail™ Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: Acetonitrile:Acetone (75:25) B: Water Gradient: Time: 0 18 %B: 24 50 Flowrate: 1.0mL/min Column Temp: Ambient Detector: ELSD 2000

Column:

0

10

20

30

40 Min.

0

6

12

18 Min.

○

○

○

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9. Maltononaose 10. Maltodecaose 11. Maltoundecaose 12. Maltododecaose 13. Maltotridecaose 14. Maltotetradecaose 15. Maltopentadecaose

400

Figure 2 - Prevail™ Carbohydrate ES Column and the ELSD 2000 permit the use of solvents chosen for their chromatography advantages instead of their spectral properties. Here, the use of acetone in the mobile phase results in a 50% reduction in runtime. © Copyright 2003 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.

Alltech Prevail™ Carbohydrate ES Column Glucooligosaccharides in Maltodextrin 9534

2 3 1. Glucose (DP1) 2. DP3 (1,6-alpha; 1,4-alpha) 3. DP6 (1,6-alpha; 1,4-alpha) 4. DP13 5. DP18 Prevail™ Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 50 60 %B: 35 60 60 Flowrate: 1.0mL/min Column Temp: Ambient Detector: ELSD 2000 Column:

Sugar Phosphates

Sugar Alcohols

1 2

9352

9544

3

3

1. Iso-erythritol 2. Xylitol 3. Mannitol 4. Maltitol

4

1. Glucose-1-phosphate 2. Fructose-6-phosphate 3. Glucose-6-phosphate X. Unknown

4

6

8

10

12 Min.

Prevail™ Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Acetonitrile:Water (70:30) 1.0mL/min Ambient ELSD 2000

Mobile Phase: Flowrate: Column Temp: Detector:

Vegetable Fermentation Broth Standards 10004

7 1 2 4

Prevail™ Columns INDUSTRY STANDARD WATERS PART NO. PART NO.

150 x 4.6mm 250 x 4.6mm

— —

6 PRICE

5

35102 35101

9

0 PART SIZE

Carbohydrate ES 5µm 5µm

11

5 10 15 20 25 30 35 40 45 50 Min.

○

Prevail™ Rocket™ Columns PACKING

10

8

Column: LENGTH X ID

PART NO.

53 x 7mm 100 x 7mm

35104 35103

PRICE

Contact your Alltech office or distributor for current or local prices.

Mobile Phase: Gradient: Flowrate: Column Temp: Detector:

Prevail™ Carbohydrate ES, 250 x 4.6mm (Part No. 35101) A: Acetonitrile B: 0.04% NH4OH Time: 0 60 %B: 17 35 1.0mL/min Ambient ELSD 2000 © Copyright 2003 Alltech Associates, Inc.

○

Carbohydrate ES 5µm 5µm

LENGTH X ID

○

PARTICLE SIZE

○

PACKING

1. iso-erythritol 2. Fructose 3. Sorbitol 4. Mannitol 5. Glucose 6. Inositol 7. Sucrose 8. Maltitol 9. Maltose 10. Raffinose 11. Maltotriose

hplc hplc

3

○

Mobile Phase: Flowrate: Column Temp: Detector:

2

○

0

Column:

○

14 Min.

○

12

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10

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8

Prevail™ Carbohydrate ES, 250 x 4.6mm (Part No. 35101) Acetonitrile:5% Ammonium Carbonate (60:40) 1.0mL/min Ambient ELSD 2000

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6

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4

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2

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0

Column:

○

2 1

○

36 Min.

○

30

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24

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x

18

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12

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6

5

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0

4

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1

401

Alltech Prevail™ Organic Acid Column • A New Performance Standard for Organic Analysis • Silica-Based Column for Maximum Efficiency and High Resolution • Short Run Times and High Sample Throughput at Ambient Temperature • Acid Stable Media for Long Column Lifetimes • Lower Cost than Polymeric Organic Acid Columns

Figure 1 - Organic Acids 9384

8

1

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

11

Prevail™ Organic Acid (OA) columns provide unsurpassed resolution of common organic acids. The silica-based media operates in a reversed-phase mode and offers much greater column efficiency and higher resolutions than ion-exclusion columns packed with sulfonated polystyrene-divinylbenzene. The Prevail™ OA column does not require a column heater and typically operates at a significantly lower backpressure than the competitive polymeric columns.

2

4

3

5

6

7 9

The ruggedness of silica allows higher flow rates for shortened run times. As shown in Figure 1, the Prevail™ OA column can separate 11 short-chain organic acids in less than 6 minutes. This is half the time the same analysis would take on a polymeric column, effectively doubling your sample throughput. As an added bonus, the peaks are sharper and better resolved, and the columns show greater sensitivity than do polymeric columns.

0

1

2

3

10

4

5

6

Oxalic Acid Tartaric Acid Formic Acid Malic Acid Lactic Acid Acetic Acid Diglycollic Acid Maleic Acid Citric Acid Succinic Acid Fumaric Acid

7

8 Min.

Prevail™ Organic Acid, 5µm, 150 x 4.6mm (Part No. 88640) Mobile Phase: 25mM KH2PO4, pH 2.5 Flowrate: 1.5mL/min Detector: UV at 210nm Column:

pH can be used to great advantage to adjust column selectivity. Lowering the pH progressively suppresses the ionization of the di-and tri-carboxylic acids, making them more hydrophobic and giving the analyst a means to move these peaks relative to other peaks in the chromatogram. With Prevail™ OA, selectivity is predictable and easily manipulated to simplify method development.

Organic Acids in Rye Bread 5

All Prevail™ Organic Acid columns are specially tested with a mixture of organic acids standards and are unconditionally guaranteed for this application.

9484

1

1. Tartaric Acid 2. Malic Acid 3. Lactic Acid 4. Citric Acid 5. Fumaric Acid

2 4 3

Prevail™ Organic Acid Analytical Columns PHASE

hplc hplc

OA

SIZE

PARTICLE LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

88650 88655 88640 88645

88750 88755 88740 88745

0

2

4

10 Min.

Prevail™ Organic Acid, 5µm, 150 x 4.6mm (Part No. 88640) Mobile Phase: 25mM KH2PO4, pH 2.5 Flowrate: 1.0mL/min Detector: UV at 210nm

Lactic Acid in Sour Cream

PARTICLE SIZE

LENGTH X ID

PART NO.

3µm

53 x 7mm

50755

PRICE

9482

1

1. Lactic Acid

Prevail™ OA All-Guard Cartridges* PHASE

PARTICLE SIZE

LENGTH X ID

All-Guard Cartridges* OA 5µm 7.5 x 4.6mm

QTY

PART NO.

3

96429

Each

80101

PRICE

○

* Requires Guard Cartridge Holder

0

1

2

3

4

5

6

7 Min.

○

All-Guard Cartridge Holder

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OA

8

Column:

Prevail™ Organic Acid Rocket™ Column PHASE

6

PRICE

○

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Column:

402

© Copyright 2003 Alltech Associates, Inc.

Prevail Organic Acid, 5µm, 150 x 4.6mm (Part No. 88640) Mobile Phase:25mM KH2PO4, pH2.5 Flowrate: 1mL/min Detector: UV at 210 Contact your Alltech office or distributor for current or local prices.

Alltech ProSphere™ — Silica Based ProSphere™ Silica Based HPLC Columns for Protein and Peptide Analysis

ProSphere™ Silica Based HPLC Columns

Prosphere™ is available in 300Å and 100Å pore sizes. 300Å pore size is recommended for routine protein and peptide analysis and offers good resolution for a wide range of molecular weights. Use 100Å pore size, when maximum resolution is needed for small peptide analysis, such as tryptic digests. Column hardware formats ranging from capillary to preparative sizes address a variety of application needs.

ProSphere™ Specifications DESCRIPTION

C4

Base Material:

Spherical Silica

Spherical Silica

Endcapped: Pore Size: Particle Size: Surface Area: Carbon Load: Phase Type:

Yes

Yes 300Å 5,10µm 100m2/g 3% Polymeric

100Å 5µm 350m2/g 15% Monomeric

300Å 5,10µm 100m2/g 9% Polymeric

C4

ProSphere™ All-Guard™ Cartridges* DESCRIPTION

PARTICLE SIZE

QTY

PART NO.

5µm 5µm 5µm

3 3 3

34003 34005 35095

Ea

80101

PRICE

™

5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

50mm x 75µm 150mm x 75µm 250mm x 75µm 50mm x 150µm 150mm x 150µm 250mm x 150µm 50mm x 300µm 150mm x 300µm 250mm x 300µm 150mm x 4.6mm 250mm x 4.6mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm

32900 32901 32902 32903 32904 32905 32906 32907 32908 32969 32971 35085 32972 35071 32973

300Å 300Å 300Å 300Å

10µm 10µm 10µm 10µm

100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm

35093 32743 35106 32744

300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å

5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

50mm x 75µm 150mm x 75µm 250mm x 75µm 50mm x 150µm 150mm x 150µm 250mm x 150µm 50mm x 300µm 150mmx 300µm 250mm x 300µm 150mm x 4.6mm 250mm x 4.6mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm

33900 33901 33902 33903 33904 33905 33906 33907 33908 33989 33991 35109 33992 35057 33993

300Å 300Å 300Å 300Å

10µm 10µm 10µm 10µm

100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm

35075 32749 35084 32752

○ ○ ○ ○

○

○

○

For additional information, including polymer based phases and applications, please refer to the Life Science section on pages 566-571.

○

© Copyright 2003 Alltech Associates, Inc.

○

Contact your Alltech office or distributor for current or local prices.

○

○

○

i

○

○

○

All-Guard™ Cartridge Holder * Requires Guard Cartridge Holder

○

○

All-Guard Cartridges C18, 7.5 x 4.6mm C4, 7.5 x 4.6mm C18, 7.5 x 4.6mm 100Å

300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å 300Å

PRICE

hplc hplc

C18

35500 35501 35502 35503 35504 35505 35506 35507 35508 35091 35087 35059 35097 35073 35079

○

ProSphere™ silica is base-deactivated and thoroughly endcapped following bonding. These processes minimize acidic silanols which often cause poor resolution and low recoveries. ProSphere™ recoveries are high—typically well above 90 percent.

50mm x 75µm 150mm x 75µm 250mm x 75µm 50mm x 150µm 150mm x 150µm 250mm x 150µm 50mm x 300µm 150mm x 300µm 250mm x 300µm 150mm x 4.6mm 250mm x 4.6mm 100mm x 10mm 250mm x 10mm 100mm x 22mm 250mm x 22mm

○

Polymerically bonded C18 phases are stable, even in the harsh mobile phases common to biomolecule assays. Continuous washing with 0.3% v/v trifluoroacetic acid for 530 hours at 30° C did not affect capacity factor or resolution. Please refer to page 566 for more information regarding this study.

5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

○

Stable bonded phases, excellent resolution, and high protein recoveries make Alltech’s ProSphere™ columns an ideal choice for your biomolecule assays. ProSphere™ silica is low in metals and base-deactivated, providing optimum peak shape and maximum protein recoveries. ProSphere™ columns can be used to identify, quantitate, or purify peptides and proteins. Typical applications include small bioactive peptides, insulin, tryptic digests, and large proteins.

LENGTH X ID

100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å

○

C18

INDUSTRY STANDARD PART NO.

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PACKING

PARTICLE SIZE

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• Stable Bonded Phases • Excellent Resolution and Recoveries • 100Å and 300Å Pore Sizes • Capillary to Preparative Formats

PORE SIZE

403

AKZO Nobel Kromasil™ Kromasil™ HPLC Columns

AKZO Nobel Kromasil™ Guard Columns*

• High Purity Silica with Low Metal Content • 5µm to 16µm Spherical Particles for Analytical to Preparative Scale Separations • High Carbon Load For Better Separation of Structurally Related Components Kromasil™ is a highly pure, spherical silica with metal impurity levels less than 10ppm. This low metal content reduces the presence of acidic silanols on the silica surface that cause peak tailing for basic compounds. Kromasil™ is available in 5-, 7-, 10-, 13-, and 16-µm particle sizes making it a good choice for scaling up to preparative HPLC. First start with an analytical column to develop the method and determine overload conditions. Then increase the column diameter to increase the column loading capacity.

DESCRIPTION

PARTICLE SIZE

LENGTH X ID

All-Guard™ Cartridges C18 5µm C18 5µm C8 5µm C8 5µm C4 5µm NH2 5µm Silica 100Å 5µm

7.5 x 3.2mm 7.5 x 4.6mm 7.5 x 3.2mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm

All-Guard™ Cartridge Holder

PART NO.

3 3 3 3 3 3 3

96371 96163 96372 96164 96165 96166 96167

Ea

80101

PRICE

* Requires Guard Holder

All Kromasil™ columns are packed by Alltech Associates in a variety of column formats and dimensions. If the column or particle size you need is not listed, please see the Custom Packed HPLC section in this catalog or call Alltech Technical Support.

Procainamide and N-Acetylprocainamide 1

7342

2 Kromasil™ Column Specifications Pore Size: Particle Shape: Carbon Load C18: Carbon Load C8:

QTY

1. Procainamide 2. N-Acetylprocainamide

100Å Spherical 19%, 3.2µm mole/m2 12%, 3.6µm mole/m2

AKZO Nobel Kromasil™ Columns WATERS INSTRUMENT PART NO.

5µm 5µm 5µm 5µm 10µm

150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm

62601 62625 62701 62000 62002

62603 62627 62709 62646 62648

5µm 5µm 5µm 5µm 10µm

150 x 3.2mm 250 x 3.2mm 150 x 4.6mm 250 x 4.6mm 250 x 4.6mm

62628 62630 62703 62005 62007

62631 62629 62711 62650 62652

C18

C8

hplc hplc

PACKING

C4

5µm 10µm

250 x 4.6mm 250 x 4.6mm

62632 62634

62654 62656

NH2

5µm 10µm

250 x 4.6mm 250 x 4.6mm

62640 62642

62662 62664

Si-100

5µm 10µm

250 x 4.6mm 250 x 4.6mm

62010 62012

62666 62668

PRICE

1

2

3

4

5

6

7 Min.

Column: Kromasil™ C8, 10µm, 250 x 4.6mm Mobile Phase: 0.025M KH2PO4, pH 3.5:Acetonitrile (93:7) Flowrate: 2mL/min Detector: UV at 254nm

Ethyl- and n-Propylbenzene 7341

1. Ethylbenzene 2. n-Propylbenzene 1

2

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Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

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INDUSTRY STANDARD PART NO.

PARTICLE SIZE

© Copyright 2003 Alltech Associates, Inc.

4

6

8

10

12 Min.

Kromasil™ C8, 10µm, 250 x 4.6mm Acetonitrile:Water (70:30) 2mL/min UV at 254nm

Contact your Alltech office or distributor for current or local prices.

Astec Vinyl Amino and Reversed-Phase Astec Amino and Reversed-Phase Columns

Mono-, Di-, and Trisaccharides

• Durable Vinyl Alcohol Copolymer Beads • Stable Column Performance • Long Column Life • Stability from pH 2-13

9103

2

Long life and stable column performance are the result of Astec's small (5µm), durable polyvinyl alcohol copolymer beads which provide mechanical strength, chemical stability, and high efficiencies. In addition, the durable base material and stable surface chemistry allow the columns to be used over a wide pH range (pH 2-13).

1

0

Astec Amino Columns provide longer column life and more stable retention times than silica-based amino columns. Silica-based amino columns lose retention over time, perhaps due to selfhydrolysis of the bonded phase by the basic amino group, whereas Astec's strong alkaline compatible polymer is resistant to this problem (see Figure 1).

34 5

Astec Amino Columns • Optimized for the Separation of Mono- and Oligosaccharides.

1. Rhamnose 2. Fructose 3. Glucose 4. Sucrose 5. Maltose 6. Raffinose

Column: Mobile Phase: Flowrate: Detector:

10

6

20

30 Min.

Astec Amino, 5µ, 250 x 4.6mm, Acetonitrile:Water (75:25) 1.0mL/min RI

Tropane Alkaloids at Basic pH 1 9104

Astec Reversed-Phase Columns • Ideal for Amines • High Recovery of Proteins and Peptides Astec Reversed-Phase Columns are ideal for amines and other basic analytes. Packed with polyvinyl alcohol copolymer beads, the columns are free of silanols, therefore elution order is always based on the hydrophobicity of the analyte rather than polar group interaction with the base material. In addition, the polymer's pH stability allows separation of bases in their unionized form by running at basic pH (see Chromatogram 9104). Astec RP columns are also good for biomolecules. Due to exceptionally low non-specific adsorption, mass recovery of proteins and peptides is typically higher than 90% even at low sample loads.

LENGTH X ID

PART NO.

Amino

300Å

5µm

250 x 4.6mm

16012

C18

300Å 300Å

5µm 5µm

150 x 4.6mm 250 x 4.6mm

16004 16005

C8

300Å 300Å

5µm 5µm

150 x 4.6mm 250 x 4.6mm

16006 16007

C4

300Å 300Å

5µm 5µm

150 x 4.6mm 250 x 4.6mm

16008 16009

PRICE

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Contact your Alltech office or distributor for current or local prices.

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LENGTH X ID

QTY

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10 x 4.6mm 10 x 4.6mm 10 x 4.6mm 10 x 4.6mm

Ea Ea Ea Ea

28187 28180 28181 28182

PRICE

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PARTICLE SIZE

© Copyright 2003 Alltech Associates, Inc.

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Astec Guard Columns Amino 5µm C18 5µm C8 5µm C4 5µm

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DESCRIPTION

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Figure 1 - A polymer-based Astec Amino Column provides reproducible performance while a silica-based amino column loses retention with use.

Astec Amino & Reversed-Phase Guard Columns

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Acetonitrile:Water (75:25) 1.0mL/min 30°C RI Fructose, Glucose, Sucrose, Maltose

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0645

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120 200 Hr.

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40

Cumulative Flow Time Mobile Phase: Flowrate: Temp: Detector: Sample:

PACKING

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120 200 Hr.

Column: Astec C18, 5µm, 150 x 4.6mm Mobile Phase: 50mM Sodium Phosphate, pH 10:Acetonitrile (70:30) Flowrate: 0.6mL/min Detector: UV at 210nm

Astec Amino & Reversed-Phase Columns

Silica-Based Amino Column 18 16 14 12 10 8

40

10 Min.

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Retention Time (Min.)

18 16 14 12 10 8

0

hplc hplc

Astec Amino (Polymer Based Column)

1. Scopolamine 2. Atropine

2

405

Astec Cyclobond™ Astec Cyclobond™ for Chiral Separations • Versatile Chiral Selectors • Broad Range of Selectivities • Guaranteed Batch to Batch Reproducibility Cyclobond™ is the name given to the technology of bonding cyclodextrins to a high purity 5µm silica gel. This patented process produces a stable ether linkage with the cyclodextrin arranged to retain its most valuable property of forming inclusion complexes. The uniqueness of Cyclobond™ lies in the high degree of selectivity of the inclusion mechanism and the hydrogen bonding effects of the hydrophilic surface. In addition, the columns can function in several different modes including normal-, reversed-, and polar organic phase modes. The range of appropriate separations is as broad as for a C18, though by a totally different mechanism. Three native cyclodextrin phases and eight derivatives are available as shown in Table 1. The Cyclobond™ I 2000 series, based on the beta cyclodextrin form, has shown the broadest applicability. It demonstrates the greatest suitability for small analytes of interest in the pharmaceutical, chemical, and environmental areas. Cyclobond™ I 2000 is a second generation product specially designed to meet the stringent requirements for analysis in the pharmaceutical industry. These phases offer guaranteed batch to batch reproducibility, excellent stability and improved selectivity and resolution. The naphthylethyl carbamate derivatives (Cyclobond™ I 2000 SN and RN) are the most versatile phases in the product line with the ability to separate a broad range of compounds. They can operate in the normal phase, reversed-phase, or polar organic phase modes, each mode offering a different selectivity. Cyclobond™ I 2000 RSP is a general purpose chiral stationary phase. It has the ability to separate non-aromatic structures such as t-boc amino acids for which it is a standard methodology. Cyclobond™ I 2000 Ac is the preacetylated beta-cyclodextrin. It is used primarily for aromatic alcohols or amines that are chiral on the alpha or beta carbon.

hplc hplc

Cyclobond™ I 2000 DMP has shown good selectivity when the chiral centers are part of a ring structure or on the alpha carbon. This phase has been very useful for derivatized amines such as amphetamine ACQ. Cyclobond™ II, is the gamma-cyclodextrin form. It is useful for isomeric compounds based on anthracene, chrysene, and pyrene type ring structures. Some applications include steroids, porphyrins, and FMOC amino acids. Cyclobond III, the alpha-cyclodextrin form, is an excellent selector for single ring structures. The smaller diameter cyclodextrin cavity makes it most useful for molecules smaller than benzene, many underivatized amino acids, prostaglandins, and inorganic ions. Table 1 - Cyclodextrin Phases DESIGNATION

SUBSTITUENT

I 2000: I 2000 Ac: I 2000 SP: I 2000 RSP: I 2000 SN: I 2000 RN: I 2000 DMP: II: II Ac: III: III Ac:

β-Cyclodextrin β-Cyclodextrin Acetylate β-Cyclodextrin S-Hydroxypropyl Ether β-Cyclodextrin R,S-Hydroxypropyl Ether β-Cyclodextrin S-Napthyl Ethyl Carbamate β-Cyclodextrin R-Napthyl Ethyl Carbamate β-Cyclodextrin 3,5-Dimethylphenyl Carbamate γ-Cyclodextrin γ-Cyclodextrin Acetylate α-Cyclodextrin α-Cyclodextrin Acetylate

PARTICLE SIZE

PACKING

LENGTH X ID

INDUSTRY STANDARDS PART NO.

I 2000

5µm 5µm 5µm

50 x 4.6mm 100 x 4.6mm 250 x 4.6mm

400102 400101 410101

I 2000 Ac

5µm 5µm

250 x 4.6mm 500 x 4.6mm

410121 410131

I 2000 SP

5µm 5µm 5µm

50 x 4.6mm 250 x 4.6mm 500 x 4.6mm

400103 411111 411211

I 2000 RSP

5µm 5µm

250 x 4.6mm 500 x 4.6mm

411121 411221

I 2000 SN

5µm 5µm 5µm

50 x 4.6mm 250 x 4.6mm 500 x 4.6mm

400104 411161 411261

I 2000 RN

5µm 5µm

250 x 4.6mm 500 x 4.6mm

411171 411271

I 2000 DMP

5µm 5µm

250 x 4.6mm 500 x 4.6mm

412111 412211

II

5µm 5µm 5µm 5µm

50 x 4.6mm 100 x 4.6mm 250 x 4.6mm 500 x 4.6mm

400105 400201 410201 400231

II Ac

5µm 5µm

250 x 4.6mm 500 x 4.6mm

410221 410231

III

5µm 5µm 5µm

100 x 4.6mm 250 x 4.6mm 500 x 4.6mm

400301 410301 400331

III Ac

5µm 5µm

250 x 4.6mm 500 x 4.6mm

410321 410331

PRICE

Astec Cyclobond™ Guard Cartridges* PACKING

PARTICLE SIZE

Guard Cartridges I 2000 5µm I 2000 Ac 5µm I 2000 SP 5µm I 2000 RSP 5µm I 2000 SN 5µm I 2000 RN 5µm I 2000 DMP 5µm II 5µm II Ac 5µm III 5µm III Ac 5µm

LENGTH X ID

20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm 20 x 4.0mm

Astec Guard Cartridge Holder

PART NO.

PRICE

430102 430103 430104 430105 430106 430107 430108 430109 430110 430112 430113 11014

*Requires Guard Holder

i

Semi-Preparative and Preparative Scale Columns are available, contact Alltech Customer Service.

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Astec Cyclobond™ Columns

406

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Astec Chirobiotic™ Naproxen

Chirobiotic™ HPLC Columns for Chiral Separations

8769

CH3

• Bonded Macrocyclic Glycopeptide Phases • 3 Complementary Selectivities • Efficient 5µm Particles • Effective in Multiple Modes

Chirobiotic R™

4 6 8 10 12 Min. Column: Chirobiotic T™, 250 x 4.6mm Mobile Phase: Ethanol:Water (80:20) Flowrate: 1.0mL/min Detector: UV at 210nm

Astec Chirobiotic™ Columns PACKING

PARTICLE SIZE

LENGTH X ID

INDUSTRY STANDARDS PART NO.

™

5µm

50 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 10.0mm

11346 11023 11024 11034

Chirobiotic T™

5µm

50 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 10.0mm

11349 12051 12053 12085

Chirobiotic R™

5µm

50 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 10.0mm

12514 12516 12518 12520

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Chirobiotic V

PRICE

Astec Chirobiotic™ Guard Cartridges* PACKING

LENGTH X ID

PART NO.

PRICE

Guard Cartridges (Requires Astec Guard Holder) Chirobiotic V™ 20 x 4.0mm 11019 Chirobiotic T™ 20 x 4.0mm 12445 Chirobiotic R™ 20 x 4.0mm 12522 11014

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Semi-Preparative and Preparative scale columns are available, contact Alltech Customer Service.

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* Requires Guard Holder

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Astec Guard Cartridge Holder

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Chirobiotic R™ is Astec's newest introduction in this line. It is based on covalently bonded Ristocetin A, the largest and most complex of the Chirobiotic™ ligands. Ristocetin A has 38 chiral centers surrounding 4 cavities. Six sugar moieties, a peptide chain, and additional ionizable groups give this structure the complexity and diversity to separate a wide variety of analytes.

○

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Ideal For: • Anionic Molecules, Di-and Tri-peptides, α-hydroxy Acids,Substituted Aliphatic Acids, Aromatic Esters, Chiral Alcohol, and Secondary and Tertiary Amines

OH NH2

○

Chirobiotic T™, Astec's second Chirobiotic™ phase, is based on Teicoplanin. It has 23 chiral centers surrounding four cavities. Hydrogen donor and acceptor sites are readily available close to seven aromatic rings. Chirobiotic T™ is an excellent alternative to crown ether and ligand exchange for amino acids and hydroxy acids.

O

hplc hplc

Ideal For: • Underivatized Amino Acids, N-derivatized Amino Acids, Carboxylic Acids, Phenols, Small Peptides, Neutral Aromatics, and Cyclic Aromatic and Aliphatic Amines

8770

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Chirobiotic T™

Phenylalanine

○

Chirobiotic V™ is the first of Astec's Chirobiotic™ phases. The Vancomycin bonded phase contains 18 chiral centers surrounding 3 cavities that are bridged by five aromatic ring structures. Hydrogen donor and acceptor sites are readily available close to the ring structures. Chirobiotic V™ has demonstrated selectivity similar to glycoprotein phases with the added advantage that it is stable from 0-100% organic modifier and exhibits higher sample capacity.

Column: Chirobiotic V™, 250 x 4.6mm Mobile Phase: THF:0.1% Triethylammonium Acetate (TEAA), pH 7 (10:90) Flowrate: 1.0mL/min Detector: UV at 254nm

○

Ideal for: • Neutral Molecules, Amides, Acids, Esters, and Cyclic Amines

H3CO

10 12 Min.

○

Chirobiotic V

™

8

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Chirobiotic™ Columns have demonstrated a broad selectivity in 3 different modes of operation: reversed-phase, normal phase, and polar organic phase modes. Enantio-selectivity differs in each of these modes, giving Chirobiotic™ Columns the ability to separate a greater variety of chiral analytes than columns that operate in only 1 mode. Chirobiotic™ Columns do not need to be dedicated to one mode of operation, as the stationary phase is unaffected when switching between different mobile phase systems.

6

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Three unique Chirobiotic™ phases are now available: Chirobiotic V™ (Vancomycin), Chirobiotic T™ (Teicoplanin), and Chirobiotic R™ (Ristocetin A). Each of these chiral phases has unique selectivity characteristics that are complementary to each other. This means an analyte unable to be sufficiently resolved on one Chirobiotic™ phase, can likely be baseline resolved on one of the others.

O

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Astec's Chirobiotic™ Columns offer a relatively new technology for chiral separations. The technology is based on covalently bonding macrocyclic glycopeptides to a high purity, 5µm-spherical silica gel. The chiral ligands are attached by multiple linkages in such a way as to establish stability while retaining essential components for chiral recognition.

CHCOH

407

Beckman Ultrasphere® Ultrasphere® Normal and Reversed Phase Columns

Tricyclic Antidepressants 7739

• Narrow Particle Size Range for Highest Efficiency • Maximum Surface Coverage for Greater Resolving Power and Long Column Life • Exhaustively Endcapped to Eliminate Unreacted Silanols • Low Metal Content for Symmetrical Peaks • Tightly Controlled Manufacturing for Batch to Batch Consistency Ultrasphere® Columns from Beckman are the basis of a number of established methods. The columns are available from Alltech in a variety of bonded phases and column formats. Ultrasphere® columns are produced on silica with a narrow particle size range. Maximum surface coverage of the bonded phase results in highly retentive columns with a high degree of resolving power. In addition, each Ultrasphere® bonded phase is exhaustively endcapped to eliminate the mixed mode interactions caused by unreacted silanols that lead to poor symmetry. Endcapping and high surface coverage protect the underlying silica for enhanced column lifetime.

1. 2. 3. 4. 5. 6. 7.

1 7 2 34 5

0

Doxepin Desipramine Nortriptyline Imipramine Amitriptyline Trimipramine Clomipramine

6

5

10

15 Min.

Column:

Ultrasphere C18, 5µm, 150 x 4.6mm (Part No. 235330) Mobile Phase: 10mM Triethylamine, pH2.5:Acetonitrile (71:29) Flowrate: 2.0mL/min Detector: UV at 216nm

Caffeine Metabolites 7736

Beckman Ultrasphere® Columns PACKING

C18

C18-IP

hplc hplc

C8

LENGTH X ID

3µm 5µm 5µm 5µm 5µm 5µm

75 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm 150 x 2.0mm 250 x 2.0mm

244254 235330 235329 235328 237390 244434

5µm 5µm

150 x 4.6mm 250 x 4.6mm

235334 235335

3µm 5µm 5µm 5µm 5µm 5µm

75 x 4.6mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm 150 x 2.0mm 250 x 2.0mm

244228 235333 235332 235331 237395 237396

3µm 5µm 5µm 5µm 5µm 5µm

75 x 4.6mm 150 x 2.0mm 250 x 2.0mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm

237573 237393 237394 244070 244071 244073

3µm 5µm 5µm 5µm 5µm 5µm

75 x 4.6mm 150 x 2.0mm 250 x 2.0mm 150 x 4.6mm 250 x 4.6mm 250 x 10mm

237574 237391 237392 235342 235341 235340

Silica

1. 7-Methylxanthine 2. 1-Methyl Uric Acid 3. 3-Methylxanthine 4. 1-Methylxanthine 5. 1,3-Dimethyl Uric Acid 6. 3,7-Dimethylxanthine 7. 1,7-Dimethyl Uric Acid 8. 1,7-Dimethylxanthine 9. 1,3-Dimethylxanthine

1 PRICE

8 7

3

56 4 9

2

0

3

6

Column: Mobile Phase: Flowrate: Detector:

9

12

15

18

21 Min.

Ultrasphere® C18, 5µm, 250 x 4.6mm 0.05% Acetic Acid: Methanol (92:8) 1.0mL/min UV at 273nm

Beckman Ultrasphere® Guard Columns* DESCRIPTION

PARTICLE SIZE

All-Guard™ Cartridges C18 5µm C18-IP 5µm C8 5µm CN 5µm Silica 5µm

LENGTH X ID

QTY

PART NO.

7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm

3 3 3 3 3

96279 96280 96281 96282 96283

Ea

80101

All-Guard Cartridge Holder

PRICE

*Requires Guard Holder

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CN

PARTICLE SIZE

INDUSTRY STANDARD PART NO.

408

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Brownlee™ Cartridge Hardware Brownlee™ MPLC® Cartridge Hardware

Brownlee™ MPLC® Cartridge Hardware PHOTO

• Easy to Use — Finger-Tight Modular Design • Lower Cost — Reusable End Assemblies and Interchangeable Holders • Versatile — Couple Analytical Cartridges or NewGuard® and Analytical Cartridges The patented Brownlee™ MPLC® Cartridge system ensures leak-free operation to 7000 psig, with only finger-tightening. Reusable end assemblies and interchangeable holders make the cartridge system less expensive than conventional columns. Changing MPLC® Cartridges is easy. No wrenches are required, and there is no need to disconnect tubing from your LC system. Simply loosen the end assembly with the tubing attached, slide out the cartridge column, and replace it with a new one. MPLC® Holder bodies come in lengths of 30-. 100-, 150-, 200-, and 250mm, and accommodate both 4.6 and 2.1mm ID cartridges. To begin using the Brownlee™ MPLC® Cartridge System, simply order a cartridge and MPLC® Cartridge holder (A or B) of the same length. The cartridge holder includes two end assemblies, a holder body, and two sets of nuts and ferrules.

DESCRIPTION

PART NO.

MPLC® Cartridge Holders A 30mm B 100mm B 150mm B 220mm B 250mm

140200 140230 140205 140400 140210

MPLC® Cartridge Accessories C MPLC® Cartridge Union D MPLC® Cartridge End Assembly

140300 1402501

MPLC® Cartridge Holder Bodies E 30mm E 100mm E 220mm

140203 140003 140403

PRICE

Universal MPLC® Cartridge Holder Kit Universal MPLC® Cartridge Holder Kit 140530 (Includes: 30-, 100-, and 220mm MPLC® Cartridge Holder Bodies (E), 1 MPLC® Cartridge Union (C), 2 End Assemblies (D), and 1 NewGuard® End Assembly (H)

5424

Brownlee™ NewGuard® Cartridges

A

B

NewGuard® Cartridges protect your analytical columns and can extend column lifetime without any loss in efficiency. NewGuard® cartridges are 1.5cm x 3.2mm and are available with 11 different packings. Attach NewGuard® Cartridges to conventional columns or cartridge column holders by using a NewGuard® Cartridge Holder (F) and connecting tubing. For direct attachment of a NewGuard® Cartridge to an analytical cartridge, a NewGuard® System Holder (G) can be used. NewGuard® Cartridges are available in packages of 3.

0647A

0647B

0647C

Brownlee™ MPLC® NewGuard Hardware

D

C

PHOTO

F

0647D

E G

F

PRICE

140601

NewGuard® System Holders 220mm NewGuard® System Holder for Direct Coupling of 220mm Analytical Cartridge with a NewGuard® Cartridge NewGuard® Accessories NewGuard® End Assembly

140410

140600 ○

H

PART NO.

NewGuard® Cartridge Holders NewGuard Holder for use with Conventional Columns

hplc hplc

0647E

DESCRIPTION

QTY

PART NO.

3 3 3 3 3 3 3 3 3 3 3

141001 141002 141003 141004 141006 141007 141008 141009 141010 141011 141012

PRICE

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DESCRIPTION

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Brownlee™ NewGuard® Cartridges

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G

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0647F

*Requires New Guard Cartridge Holder (Part No. 140601)

© Copyright 2003 Alltech Associates, Inc.

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PRICE

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PART NO.

MPLC® Cartridge Holder Seals and Seal Replacement Kits Analytical Holder Seal Replacement Kit 140260 (Includes Tool and 2 Seals) Analytical Seals, 2/pkg 140216 (Seal Replacement Tool Required) Contact your Alltech office or distributor for current or local prices.

○

DESCRIPTION

○

Brownlee™ MPLC® Cartridge Hardware Seals

○

0647G

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H

NewGuard Cartridges* RP-2 RP-4 RP-8 RP-18 Phenyl Amino Cyano Anion (AX-300) Cation (CX-300) Diol Silica

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®

409

Brownlee™ Spheri-5®, and -10® Brownlee™ Spheri-5® and -10®

Brownlee™ Spheri-10® Columns†

• Wide Variety of Column Chemistries • Cartridge Convenience • Microbore and Semi-Preparative Columns Brownlee™ Columns undergo extensive physical and chemical testing to insure the highest quality and reproducibility. Spheri-5® and Spheri-10® bonded phases are based on spherical silica with 80Å pores. The phases are available in MPLC® Cartridges or conventional columns. Brownlee™ reversed-phase media include monofunctional RP-8 (C8) and RP-18 (C18) as well as polyfunctional OSS (C8) and ODS (C18) versions. The RP-8 and RP-18 Columns provide the optimum in mass transfer kinetics and high efficiency due to the monomolecular layer of bonded phase. The polyfunctional OSS and ODS phases are bulkier, durable phases suitable for use with acidic mobile phases because the complete surface coverage minimizes degradation of the silica substrate. The Phenyl material is also a popular reversed-phase media. It is more polar than C8 and C18 phases and offers a different selectivity due to its aromatic character. Amino, Cyano, and Silica comprise Brownlee’s offering of polar phases. Although cyano media is traditionally used in normal phase, with proper mobile phase conditions, this column can also be used in reversed-phase analyses.

PACKING

PARTICLE SIZE

LENGTH X ID

RP-18

10µm

250 x 4.6mm

141049

RP-2

10µm

250 x 4.6mm

141019

3

No holder necessary.

Brownlee™ Spheri-5® Cartridges* PACKING

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6

LENGTH X ID

QTY

PART NO.

5µm 5µm 5µm 5µm 5µm 5µm

30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm

2 2 Ea Ea Ea Ea

141051 141052 141053 141054 1410551 141056

ODS (C18)

5µm 5µm 5µm 5µm 5µm 5µm

30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm

2 2 Ea Ea Ea Ea

141060 141061 141062 141063 141064 141065

RP-8

5µm 5µm 5µm 5µm 5µm 5µm

30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm

2 2 Ea Ea Ea Ea

141027 141028 141029 141030 141031 141032

9

12

15 Min.

1. 2. 3. 4. 5. 6. 7.

Procainamide Acetylprocainamide Nadolol Lidocaine Disopyramide Propranolol Nifedipine

OSS (C8)

5µm

100 x 4.6mm

Ea 141037

Phenyl

5µm 5µm 5µm

30 x 4.6mm 100 x 4.6mm 220 x 4.6mm

2 141068 Ea 141070 Ea 141072

Cyano (CN)

5µm 5µm 5µm

30 x 4.6mm 100 x 4.6mm 220 x 4.6mm

2 141083 Ea 141085 Ea 141087

Amino (NH2) 5µm 5µm 5µm

30 x 4.6mm 100 x 4.6mm 220 x 4.6mm

2 141177 Ea 141179 Ea 141181

Silica

30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm

2 2 Ea Ea Ea

Brownlee™ Spheri-10® Cartridges* PACKING

LENGTH X ID

QTY

PART NO.

10µm 10µm

30 x 4.6mm 100 x 4.6mm

2 Ea

141020 141022

RP-8

5µm 5µm 5µm 5µm 5µm

PRICE

141193 141194 141195 141196 141197

*Brownlee™ MPLC® Cartridge Holder required. See page 409.

PARTICLE SIZE

PRICE

Brownlee™ Spheri-5® Columns† PACKING

PARTICLE SIZE

5µm

250 x 4.6mm

141033

LENGTH X ID

PART NO.

PRICE

RP-18

10µm 10µm 10µm

30 x 4.6mm 100 x 4.6mm 220 x 4.6mm

2 Ea Ea

141043 1410451 141047

RP-8 RP-18

5µm

250 x 4.6mm

141057

10µm

30 x 4.6mm

2

141186

ODS (C18)

5µm

250 x 4.6mm

141066

Silica

5µm

250 x 4.6mm

141199

Silica ™

®

*Brownlee MPLC Cartridge Holder required.

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RP-18

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Column: Spheri-5® RP-8, 220 x 2.1mm Mobile Phase: A: 0.08% Diethylamine, 0.09% Phosphoric Acid, pH 2.3 B: 0.08% Diethylamine, 0.09% Phosphoric Acid:Acetonitrile (10:90) Gradient: Time: 0 2 17 %B: 5 5 100 Flowrate: 0.5mL/min Temp: 50° C Detector: UV at 200nm

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0

23

PRICE

†

Cardiac Drugs 1

PART NO.

410

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For information on Brownlee™ MPLC® Cartridge Hardware, refer to page 409. © Copyright 2003 Alltech Associates, Inc.

†

No holder necessary.

Contact your Alltech office or distributor for current or local prices.

Brownlee™ Aquapore® and Polypore® Aquapore® Reversed-Phase and Ion-Exchange

Brownlee™ Polypore® Columns

• 300 Angstrom Pore Size for Macromolecules • Silica-Based Packing for High Efficiency • Ion-Exchange Packing for Proteins and Peptides

• pH Stable PS-DVB Packings • Ion-Exclusion Columns for Organic Acids and Carbohydrates

®

The Aquapore line of stationary phases separates large biological compounds and macromolecules. These materials are based on a 300 angstrom pore size silica to minimize exclusion of large molecules from the internal structure of the sorbent. Aquapore® reversed-phases include butyl (BU-300), octyl (RP-300), and ODS (OD-300). Aquapore® AX-300 is a silica-based weak anion exchanger made for protein and peptide chromatography. It uses a diethylaminoethyl (DEAE) functionality as the exchange group. Weak ion-exchange packings are particularly versatile because the charge on the packing, and the column capacity, can be changed by varying the mobile phase pH.

Polypore® polymer-based columns are stable over an extended pH range, making them useful with aggressive mobile phases that dissolve silica-based materials. The Polypore® base material is a 10 micron polystyrene-divinylbenzene (PS-DVB) copolymer. Polypore® H and CA are packed with a microporous sulfonated polystyrene-divinylbenzene resin in the hydrogen (H+) or calcium (Ca2+) form. Polypore® H columns are designed for organic acid separations with dilute sulfuric acid mobile phases. Polypore® CA columns separate carbohydrates using only water as the mobile phase.

Organic Acids

1 6

2

7

1. Oxalic Acid 5570 2. Citric Acid 3. α-Ketoglutaric Acid 4. Malic Acid 5. Succinic Acid 6. Formic Acid 7. Acetic Acid 8. Fumaric Acid 9. Butyric Acid

8

34 5

Brownlee™ Aquapore® Cartridges†

7µm 7µm 7µm 7µm 7µm 7µm

30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm

2 2 Ea Ea Ea Ea

141102 141103 141104 141105 141106 141107

7µm 7µm 7µm

30 x 4.6mm 30 x 2.1mm 100 x 2.1mm

2 2 Ea

141112 141113 141115

7µm 7µm 7µm 7µm 7µm 7µm

30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm

2 2 Ea Ea Ea Ea

141212 141213 141214 141215 141216 141217

RP-300 (C8)

BU-300 (C4)

PART NO.

PRICE

9

0 5 Column: Mobile Phase: Flowrate: Temp: Detector:

10 20 Min. 15 Polypore® H, 220 x 4.6mm 0.01N H2SO4 0.5mL/min 25° C UV at 210nm

Sugars 4

5571

1

3 2 5

Brownlee™ Aquapore® Columns*

6 9 Polypore® CA, 220 x 4.6mm Water 0.3mL/min 85°C RI

LENGTH X ID

PART NO.

OD-300 (C18)

7µm

250 x 4.6mm

141098

Brownlee Polypore® Cartridges*

RP-300 (C8)

7µm 7µm 7µm

100 x 1.0mm 250 x 1.0mm 250 x 4.6mm

141111 141110 141108

PACKING

7µm

250 x 4.6mm

141218

PRICE

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LENGTH X ID

QTY

PART NO.

H

10µm 10µm 10µm

30 x 4.6mm 100 x 4.6mm 220 x 4.6mm

2 Ea Ea

141248 141249 141250

CA

10µm 10µm 10µm

30 x 4.6mm 100 x 4.6mm 220 x 4.6mm

2 Ea Ea

141253 141254 141255

PRICE

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PARTICLE SIZE

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For information on Brownlee™ MPLC® Cartridge Hardware, refer to page 409. © Copyright 2003 Alltech Associates, Inc.

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*Brownlee™ MPLC® Cartridge Holder required.

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For information on Brownlee™ MPLC® Cartridge Hardware, refer to page 409.

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i

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*No holder required.

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AX-300

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PARTICLE SIZE

PACKING

12 Min.

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0 3 Column: Mobile Phase: Flowrate: Temp: Detector:

Brownlee™ MPLC® Cartridge Holder is required.

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†

6

Maltotriose Maltose Glucose Fructose Mannitol Ribose

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AX-300

1. 2. 3. 4. 5. 6.

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2 141092 2 141093 Ea 141094 Ea 1410951 Ea 141096 Ea 141097

QTY

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30 x 4.6mm 30 x 2.1mm 100 x 4.6mm 100 x 2.1mm 220 x 4.6mm 220 x 2.1mm

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LENGTH X ID

7µm 7µm 7µm 7µm 7µm 7µm

OD-300 (C18)

hplc hplc

PARTICLE SIZE

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PACKING

411

GL Sciences Inertsil® Inertsil® HPLC Columns • • • • • •

Inertsil® 3-Series columns contain an HPLC media designed specifically for difficult separations. This media has a perfectly spherical shape, a smooth particle surface, and a narrow particle size distribution resulting in lower column back pressure. This means less stress on the packing material and longer column life.

Manufactured from 99.999% Pure Silica Excellent for Basic Drugs, Amines, and Other Polar Molecules Unrivaled for Chemical and Physical Stability Excellent Reproducibility Wide Pore Packings for Biomolecules Capillary Column Formats

Where appropriate, Inertsil® 3-Series packings are fully endcapped after bonding to improve peak shape. The more popular packings are available in a 3 µm particle size for applications requiring increased resolution. The 3 µm packings can also be used in smaller column volumes to reduce analysis time (compared to 5 µm) without sacrificing resolution.

Inertsil® HPLC products represent state-of-the-art manufacturing technology and are unrivaled for their physical and chemical stability. Inertsil®’s stability is directly related to its complete uniformity. The physical characteristics of Inertsil® were designed to optimize physical stability, column efficiency, loading capacity, compatibility with large molecules, and lot-to-lot reproducibility. The uniformity of the silica, with 99.999% purity, greatly contributes to the stability of Inertsil®. Many other silicas contain large amounts of metal ions and sulfate contaminants making them subject to dissolution in aggressive mobile phases. Inertsil®, which does not contain such contaminants, shows excellent stability and long life at high and low pH levels.

Inertsil® ODS-3V columns are validated HPLC columns that can be used with complete confidence to obtain superior and reproducible chromatographic performance column after column. A number of new Inertsil® products were introduced in 2002. These include the Inertsil® phases ODS-P, ODS-EP, Diol, WP300C18, WP300-C8, WP300-Diol, and a family of capillary columns packed with all the 3-Series packing materials. The capillary columns are available in 0.3, 0.5, and 0.7 mm internal diameters and 50 and 150 mm lengths.

Inertsil® reversed-phase packings do not exhibit silanol effects towards amines or organic acids. These properties make Inertsil® an excellent choice for the analysis of basic drugs and other polar samples – the most difficult compounds to analyze on conventional reversed-phase columns.

Inertsil Packing Specifications BONDED PHASE

Inertsil® 3 Series Inertsil® ODS-3 Octadecyl Inertsil® ODS-3V Octadecyl Inertsil® ODS-P Octadecyl Inertsil® ODS-EP C18 w/Embedded Polar Inertsil® C8-3 Octyl Inertsil® Ph-3 Phenyl Inertsil® CN-3 Cyanopropyl Inertsil® NH2 Aminopropyl Inertsil® Diol Dihydroxypropyl Inertsil® Peptides C18 Octadecyl Inertsil® WP300-C18 Methyloctadecyl Inertsil® WP300-C8 Methyloctyl Inertsil® WP300-Diol Dihydroxypropyl Inertsil® 2 Series Inertsil® ODS-2 Inertsil® C8 Inertsil® C4 Inertsil® Ph Inertsil® SIL-150A

Octadecyl Octyl Butyl Phenyl —

CARBON LOAD

END CAP

PARTICLE SIZE

SURFACE AREA

PORE VOLUME

PORE SIZE

SILICA PURITY

15.0% 15.0% 29.0% 9.0% (C18) 9.0% 9.5% 14.0% 8.0% 20.0% 15.0% 8.5% 4.5% 9.5%

Yes Yes No — Yes No No No No Yes Yes Yes No

3,5,8µm 5µm 5µm 5µm 3,5,8µm 3,5,8µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 450m2/g 150m2/g 150m2/g 150m2/g

1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.05mL/g 1.12mL/g 1.12mL/g 1.12mL/g

100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 300Å 300Å 300Å

99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999% 99.999%

18.5% 10.5% 7.5% 10.0% —

Yes Yes Yes Yes No

5µm 5µm 5µm 5µm 5µm

320m2/g 320m2/g 320m2/g 320m2/g 320m2/g

1.15mL/g 1.15mL/g 1.15mL/g 1.15mL/g 1.15mL/g

150 Å 150 Å 150 Å 150 Å 150 Å

99.999% 99.999% 99.999% 99.999% 99.999%

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hplc hplc

PACKING

412

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Inertsil® HPLC Columns Inertsil® 3-Series Packings

Inertsil® 3-Series Guard Columns

3-SERIES PACKING

PORE SIZE

PARTICLE SIZE

LENGTH X ID

INDUSTRY STANDARD PART NO.

ODS-3

100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å

3µm 3µm 3µm 3µm 3µm 3µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

50 x 2.1 mm 100 x 3.0 mm 150 x 3.0 mm 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm 50 x 2.1 mm 100 x 2.1 mm 150 x 2.1 mm 150 x 3.0 mm 50 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm

GL-04412 GL-04424 GL-04425 GL-01774 GL-01771 GL-01772 GL-04512 GL-04514 GL-01741 GL-01751 GL-01763 GL-01731 GL-01732

ODS-3V

100Å 100Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-01801 GL-01802

ODS-P

100Å 100Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-02001 GL-02002

PRICE

PORE SIZE

PARTICLE SIZE

100 Å 100 Å 100 Å 100 Å 100 Å 100 Å 100 Å 100 Å 100 Å 300 Å 300 Å 300 Å

5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm 5µm

3-SERIES PACKING

ODS-3 ODS-P ODS-EP C8-3 Ph-3 CN-3 NH2 Diol Peptide C18 WP300-C18 WP300-C8 WP300-Diol

LENGTH X ID

PART NO.

Inertsil® 2-Series Packing PARTICLE SIZE

LENGTH X ID

INDUSTRY STANDARD PART NO.

WATERS INSTRUMENT PART NO.

100Å 100Å 100Å 100Å

5µm 5µm 5µm 5µm

100 x 2.1 mm 150 x 2.1 mm 150 x 4.6 mm 250 x 4.6 mm

GL-02614 GL-02615 GL-02645 GL-02646

PACKING

PORE SIZE

Silica

150Å 150Å

5µm 150 x 4.6mm 89870 5µm 250 x 4.6mm 89871

89880 89881

C8-3

100Å 100Å 100Å

3µm 5µm 5µm

150 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm

GL-01911 GL-01900 GL-01901

ODS-2

150Å 150Å 150Å

5µm 150 x 2.1mm 89890 5µm 150 x 4.6mm 89872 5µm 250 x 4.6mm 89873

— 89882 89883

Ph-3

100Å 100Å 100Å

3µm 5µm 5µm

150 x 4.6 mm 150 x 4.6 mm 250 x 4.6 mm

GL-01931 GL-01920 GL-01921

C8

150Å 150Å 150Å

5µm 150 x 2.1mm 89891 5µm 150 x 4.6mm 89874 5µm 250 x 4.6mm 89875

— 89884 89885

CN-3

100Å 100Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-01940 GL-01941

Phenyl

150Å 150Å

5µm 150 x 4.6mm 89876 5µm 250 x 4.6mm 89877

89886 89887

NH2

100Å 100Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-05545 GL-05546

Diol

100Å 100Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-05645 GL-05646

Peptide C18 100Å 100Å 100Å

5µm 5µm 5µm

250 x 2.1 mm 150 x 4.6 mm 250 x 4.6 mm

GL-08026 GL-08055 GL-08056

WP300-C18

300Å 300Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-05845 GL-05846

WP300-C8

300Å 300Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-05745 GL-05746

WP300-Diol

300Å 300Å

5µm 5µm

150 x 4.6 mm 250 x 4.6 mm

GL-05945 GL-05946

ODS-EP

PRICE

50 x 4.6mm GL815ODS346 50 x 4.6mm GL815ODSP46 50 x 4.6mm GL815ODSEP46 50 x 4.6mm GL815C8346 50 x 4.6mm GL815PH346 50 x 4.6mm GL815CN346 50 x 4.6mm GL815NH246 50 x 4.6mm GL815DIOL46 50 x 4.6mm GL81PEPTID46 50 x 4.6mm GL815WPC1846 50 x 4.6mm GL815WPC846 50 x 4.6mm GL81WPDIOL46

PRICE

Inertsil® 2-Series Guard Columns* DESCRIPTION

PARTICLE SIZE

QTY

PART NO.

7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm 7.5 x 4.6mm

3 3 3 3

96207 96204 96205 96206

Ea

80101

PRICE

All-Guard™ Guard Cartridge Holder

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© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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LENGTH X ID

hplc hplc

All-Guard Cartridges Silica 5µm ODS-2 5µm C8 5µm Phenyl 5µm

413

GL Sciences Inertsil® Aflatoxins

Inertsil® ODS-3 HPLC Columns

10144

It is well known that residual silanol groups on the surface of the silica-gel will cause severe tailing and adsorption, especially with nitrogen-containing compounds. In the production of Inertsil® ODS-3, the silanols are eliminated through a proprietary bondingendcapping technology developed at GL Sciences.

2

To ensure maximum inertness, each batch of Inertsil® ODS-3 must pass a quality control program using 29Si NMR to demonstrate isolated silanols have been completely eliminated. A long column life is one of the most important factors contributing to cost efficiency of an analysis. In general, routine use in the pH range of 2 to 7.5 is recommended for silica-based packings. The durability of Inertsil® ODS-3 at pH 2 and pH 9 has been tested. There was no change in the chromatography after 500 hours at pH 2. At pH 9 (40/60 CH3OH/Na2HPO4) there was a slight decrease in retention of the analytes after 500 hours at 1 mL/min. However, it shows adequate stability and durability at this high pH.

2

Aflatoxin G2 Aflatoxin G1 Aflatoxin B2 Aflatoxin B1

3

1

0

1. 2. 3. 4.

4

4

6

10 Min.

8

Inertsil® ODS-3V, 5µm, 150 x 4.6mm (Part No. GL-01801) Mobile Phase: (50:50) Methanol:Water Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 365 Column:

Hormones

Organic Acids

10145

8810

9

1. 2. 3. 4. 5. 6. 7. 8. 9.

123 4

0

6 5

7 8

4

8

12

Inertsil® ODS-3V, 5µm, 250 x 4.6mm (Part No. GL-01802) Mobile Phase: 0.1M Ammonium Dihydrogenphosphate, pH 2.5 Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 210nm

hplc hplc

5

12

1. β-Trenbolone 2. α-Trenbolone 3. β-Zearalenol 4. Testosterone 5. Zeranol 6. α-Zearalenol 7. α-Estradiol 8. Zearalenone 9. Diethylstilbestrol

8

34 5

9 7

0

16 Min.

Column:

3

6

Oxalic Acid Tartaric Acid Glycolic Acid Malonic Acid Lactic Acid Acetic Acid Fumaric Acid Succinic Acid Acrylic Acid

10

20 Min.

Inertsil® ODS-3, 5µm, 150 x 4.6mm (Part No. GL-01731) Mobile Phase: Acetonitrile:Tetrahydrofuran:Water (35:8:57) Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 230

Column:

Pesticides

Flavonoids 10146

8093

1. 2. 3. 4. 5. 6. 7.

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1

12

6

3 45

8 7

7

2

0

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414

1. 2. 3. 4. 5. 6. 7. 8.

Troxerutin Rutin Fisetin Morin Luteolin Quercetin Kaempferol Chrysin

4

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6

Asulam MCPP Oxine-copper Thiuram Iprodione Bensulide Pencycuron

Inertsil® ODS-3, 5µm, 150 x 4.6mm (Part No. GL-01731) Mobile Phase: Acetonitrile:50mM KH2PO4, pH 4.0 (60:40) Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 230nm © Copyright 2003 Alltech Associates, Inc. Column:

2

4

6

8 ®

10

12 14 Min.

Inertsil ODS-3, 5µm, 150 x 4.6mm (Part No. GL-01731) Mobile Phase: Methanol:Tetrahydrofuran:0.2M NaH2PO4, pH 3.0 (30:20:50) Flowrate: 1mL/min Column Temp: 40°C Detector: UV at 254 Contact your Alltech office or distributor for current or local prices. Column:

GL Sciences Inertsil® Inertsil® ODS-P HPLC Columns

Inertsil® ODS-EP HPLC Columns

Inertsil® ODS-P columns have the generic characteristics of a C18 phase with unique separation capabilities. Unlike ODS-3, which is monomeric bonding with end-capping, ODS-P is polymeric bonding without endcapping. It gives the ODS-P phase additional retentivity for planar structural compounds.

The Inertsil® ODS-EP phase is a polar-embedded phase column. It was developed to provide a column with retention close to conventional C18 columns that would tolerate highly aqueous mobile phases. Because its selectivity is quite different from conventional C18 columns, it is also useful for samples which are not separated by those columns.

Compared to generic C18 phases, Inertsil® ODS-P offers significant improvements in the separation of PAHs, carotenoids, D vitamins, and aromatic hydrocarbon isomers.

Polynuclear Aromatic Hydrocarbons 1. Naphthalene 2. Acenaphthylene 3. Acenaphthene 4. Fluorene 6

10 9

12 3

1. Formic Acid 2. Acetic Acid

1

2

16 14 15 0

0 10 Column:

20 30 40 50 Min. Inertsil® ODS-P, 5µm, 250 x 4.6mm (Part No. GL-02002) Mobile Phase: A: Methanol:Water (70:30) B: Methanol Gradient: Time: 0 5 20 %B: 0 0 100 Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 254

1

2

3

4

5 Min.

Inertsil® ODS-EP, 5µm, 150 x 4.6mm (Part No. GL-02645) Mobile Phase: 0.1% Phosphoric Acid (pH 2.2) Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 210 Column:

Commercial Divinylbenzene

Tanaka Test Sample 10160

1. 2. 3. 4. 5. 6. 7.

1 2

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4 5

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7

6

10

20 Min.

0

10

20

30

40 Min.

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Inertsil® ODS-EP, 5µm, 150 x 4.6mm (Part No. GL-02645) Mobile Phase: Methanol:Water (80:20) Flowrate: 1.0mL/min Column Temp: 40° C Detector: UV at 254 © Copyright 2003 Alltech Associates, Inc. Column:

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Inertsil® ODS-P, 5µm, 250 x 4.6mm (Part No. GL-02002) Mobile Phase: Acetonitrile:Water (65:35) Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 254 Contact your Alltech office or distributor for current or local prices.

Column:

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0

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3

Uracil Caffeine Phenol n-Butylbenzene n-Amylbenzene o-Terphenyl Triphenylene

hplc hplc

10153

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78

10159

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4

11 12 13

5. Phenanthrene 6. Anthracene 10147 7. Fluoranthene 8. Pyrene 9. benz-[a]-anthracene 10. Chrysene 11. benzo-[b]-fluoranthene 12. benzo-[k]-fluoranthene 13. benzo-[a]-pyrene 14. dibenzo-[a]-pyrene 15. benzo-[g,h,i]-pyrene 16. indeno-[1,2,3-CD]-pyrene

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5

Acid Test Sample

415

GL Sciences Inertsil® Inertsil® C8-3 HPLC Columns

Inertsil® CN-3 HPLC Columns

The same base silica and bonding technology that is used for Inertsil® ODS-3 is also employed for Inertsil® C8-3. The difference between the two phases is just the length of the hydrocarbon chains. This enables faster analyses to be performed by converting from an ODS-3 column to a C8-3 column. For example, compare the Organic Acids chromatogram on this page to that on page 414.

Historically, cyano columns have had a reputation for low reproducibility, low inertness, and short column life. Now, Inertsil® CN-3 columns change that perception. Densely bonded cyanopropyl functional groups on a pure silica surface and a unique bonding chemistry produce a column with excellent reproducibility, long lifetime, and high inertness.

The reduced retentivity of C8-3 allows a wider variation of eluent composition. For example, on ODS columns, an entirely organic eluent has to be used for the analysis of retinoids or fatsoluble vitamins. Using an Inertsil® C8-3 Column, a mixture of organic solvent and water can be used as the eluent. This introduces the possibility of pH adjustment and the addition of additives.

On ODS phases, the difference in retention of hydrophilic and hydrophobic compounds is large, and samples containing both types of compounds require long analysis times. For such samples, Inertsil® CN-3 Columns will produce a shorter analysis time. This advantage is especially compatible with the objectives of Method Development and High Throughput Screening.

Organic Acids

Catecholamines 9186

1. 2. 3. 4. 5. 6. 7. 8. 9.

9

2 1 3 5 6 7 4 8

0

1

2

3

4

5

6

7

10148

Oxalic Acid Tartaric Acid Glycolic Acid Malonic Acid Lactic Acid Acetic Acid Fumaric Acid Succinic Acid Acrylic Acid

8

9

10 Min.

Inertsil® C8-3, 5µm, 150 x 4.6mm (Part No. GL-01900) Mobile Phase: 0.1M NH4H2PO4, pH2.8 Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 210nm Column:

1. VMA 2. 5-HIAA 3. HVA

1 2 3

0

10

Inertsil® CN-3, 5µm, 150 x 4.6mm (Part No. GL-01940) Mobile Phase: (1:99) Acetonitrile:0.05M NaH2PO4, pH 3.0 Flowrate: 1.0mL/min Column Temp: 30°C Detector: ECD 700 mV vs. Ag/AgCl Column:

Phthalic Acid Dialkyl Esters

hplc hplc

1. 2. 3. 4. 5. 6. 7. 8. 9.

1

○

○

○

○

○

3, 4 2

7

6

Endocrine Disruptors

Phthalic Acid diethylester Phthalic Acid di-isopropylester 10149 Phthalic Acid di-n-butylester Phthalic Acid di-isobutylester Phthalic Acid dicyclohexylester Phthalic Acid di-n-heptylester Phthalic Acid di-2-ethylhexylester Phthalic Acid di-n-decylester Phthalic acid di-n-dodecylester

10150

4

2

6

1. 2. 3. 4. 5. 6.

10

12

5 1 3

8 9

Phenol Diethylphthalate Bisphenol-A 2,4-Dichlorophenol 4-Octylphenol 4-Nonylphenol

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5

20 Min.

416

0 Column:

10

20 Min.

0

2

4

6

8

14 Min.

®

Inertsil C8-3, 5µm, 150 x 4.6mm (Part No. GL-01900) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 5 10 %B: 75 75 100 Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 254 © Copyright 2003 Alltech Associates, Inc.

Inertsil® CN-3, 5µm, 250 x 4.6mm (Part No. GL-01941) Mobile Phase: (45:55) Acetonitrile:0.2M KH2PO4, pH 3.0 Flowrate: 1.0mL/min Column Temp: 40°C Detector: UV at 280 Contact your Alltech office or distributor for current or local prices. Column:

GL Sciences Inertsil® Inertsil® Diol and WP300-Diol HPLC Columns

Free Amino Acids 10156

3

Inertsil® Diol is a new phase produced from GL Sciences 5µm, 100Å high purity silica and a dihydroxypropyl functional group. It can be used in both reversed-phase and normalphase modes. In normal-phase mode, the retentivity is higher than that of silica, and water-acetonitrile is an effective mobile phase.

6

2 4

In the reversed-phase mode, amino acids and water-soluble vitamins can be analyzed without an ion-pair reagent in the mobile phase. The dihydroxypropyl chemistry has also been applied to the 5µm, 300Å high purity silica, to make the same unique separation modes available for resolution of larger biomolecules.

1. 2. 3. 4. 5. 6. 7.

5

1

7

0

10

Phenylalanine Methionine Valine DOPA Alanine Serine Citrulline

20 Min.

Column:

Inertsil Diol, 5µm, 250 x 4.6mm (Part No. GL-05646) Mobile Phase: (80:20) Acetonitrile:Water Flowrate: 1.0mL/min Detector: RI

Anti-inflammatories

Sugar Alcohols

10158

10157

1

3 1. Fenoprofen 2. Flurbiprofen 3. Ketoprofen

1. 2. 3. 4. 5.

2

2

1

Erythritol Xylitol Fructose Sorbitol Mannitol

5

3 4

0

2

4

6

8

10

12 Min.

0

Column:

4

6

8

10 Min.

Column:

Inertsil Diol, 5µm, 250 x 4.6mm (Part No. GL-05646) Mobile Phase: (85:15) Acetonitrile:Water Flowrate: 1.0mL/min Detector: RI

hplc hplc

Inertsil Diol, 5µm, 250 x 4.6mm (Part No. GL-05646) Mobile Phase: (90:10) Hexane:Ethanol Flowrate: 1.0 mL/min Detector: UV at 254

2

○ ○ ○

Inertsil® Sil-100

○

1

1

○

Inertsil® Diol

10154

Progesterone Corticosterone Cortisone Prednisone Prednisolone

○

1. 2. 3. 4. 5.

○

10155

○

Unique Selectivity and Retentivity of Inertsil® Diol Phase

○ ○ ○ ○ ○

5

○

4

10

20

30 Min.

0

10

20 Min.

© Copyright 2003 Alltech Associates, Inc.

○

Contact your Alltech office or distributor for current or local prices.

○

○

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○

0

○

○

3

○

2

Inertsil® Diol and Sil-100, both 150 x 4.6mm Mobile Phase: Hexane/Ethanol (85/15, w/w) Flowrate: 1.0 mL/min Column Temp: 40˚C Detection: UV at 254nm

○

2, 3 5 4

Column:

417

GL Sciences Inertsil® Tryptic Digest of BSA

Inertsil® WP300-C8 and WP300-C18 HPLC Columns

10152

Inertsil WP300-C8 and WP300-C18 Columns are new to this catalog. Along with Inertsil® WP300-Diol, they were introduced to provide the same desirable column properties of the 100Å Inertsil® materials to those scientists with samples that chromatograph better on a larger pore packing material.

Polypeptides on Wide Pore C8 10151

0

10

20

30

40 Min.

Inertsil® WP300-C8, 5µm, 250 x 4.6mm (Part No. GL-05745) Mobile Phase: A: Acetonitrile: Water 2:98 (0.1% TFA) B: Acetonitrile: Water 90:10 (0.1% TFA) Gradient: Time 0 5 50 %B 10 10 60 Flowrate: 1.0mL/min Detector: UV at 214

Column: 1. 2. 3. 4. 5. 6.

Ribonuclease (0.125 mg/mL) Insulin (0.04 mg/mL) Cytochrome (0.1 mg/mL) Lysozyme (0.13 mg/mL) BSA (0.26 mg/mL) Trypsin Inhibitor from Soy Bean (0.25 mg/mL) 7. Ovalbumin (0.25 mg/mL)

4

3

1 2

0

10

5

7

6

20

30 Min.

Inertsil® WP300-C8, 5µm, 250 x 4.6mm (Part No. GL-05745) Mobile Phase: A: Water (0.1% TFA) / B: Acetonitrile:Water 90:10 (0.1% TFA) Gradient: Time 0 25 %B 20 60 Flowrate: 1.0mL/min Detector: UV at 280

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○

○

○

○

hplc hplc

Column:

418

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Hamilton PRP®-1, -3, -X300, and -X700 Polymer-Based Hamilton PRP® Columns

Hamilton PRP® Columns

• Rugged Polystyrene Divinylbenzene (PS-DVB) Resin • 3-Micron Columns for High-Speed, High Resolution Separations • pH Stability from pH 1-13 • Mechanical Stability to 5000psig • Reversed-Phase, Normal-Phase, and Ion-Exclusion Columns Hamilton PRP® columns are based on rugged PolystyreneDivinylbenzene (PS-DVB) spheres. There is virtually no restraint on mobile phase pH as these columns can withstand pH 1-13. And they may be used with up to 100% organic modifier. Hamilton PRP® columns exhibit better mechanical stability than other PS-DVB-based columns as they have an upper pressure limit of 5000psig. PRP® columns are available in reversed-phase (PRP®-1 and PRP®-3), ion exclusion (PRP®-X300) for organic acids, and normal-phase (PRP®-X700) for sugars. PRP®-1 and PRP®-3 are polymer reversed-phases with a 100Å and 300Å pore size respectively. The extremely hydrophobic character of the PS-DVB results in behavior similar to high load C18 columns. Suggested applications for PRP®-1 columns are small molecules such as proteins and peptides (less than 1000MW), DNA purification, vitamins, artificial sweeteners, and pharmaceuticals. PRP®-3 columns are designed for purification and isolation of proteins (MW greater than 1000), peptides, and DNA oligomers. The 3 and 5 micron PRP® resins are designed for separations requiring higher resolution, while the 7 and 10 micron resins are suitable for general purpose and scale-up. Also available for scaleup are 12-20 and 30-50 micron resins. PRP®-X300 is a sulfonated PS-DVB gel in the hydrogen (H+) form that is designed for the separation of simple aliphatic carboxylic acids and alcohols. The combination of partial sulfonation and inherent hydrophobicity results in a column with a high degree of reversed-phase as well as ion-exclusion character. Inorganic anions are not retained on this column. PRP®-X700 is PS-DVB gel with an aminopropyl functionality designed for normal phase separation of carbohydrates including fructose, glucose, sucrose, and maltose. Hamilton's proprietary PS-DVB support and unique chemically bonded aminopropyl bonded phase provides long column lifetime and reproducible retention. Isocratic acetonitrile/water mobile phases make the PRP®-X700 column compatible with Refractive Index and Evaporative Light Scattering (ELSD) detectors.

PARTICLE SIZE

PACKING LENGTH X ID

PART NO.

PRP®-1

100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å 100Å

3µm 3µm 5µm 5µm 7µm 7µm 7µm 10µm 10µm 10µm

100 x 4.1mm (SS) 150 x 4.1mm (SS) 150 x 4.1mm (SS) 150 x 4.6mm (PEEK) 150 x 4.1mm (SS) 250 x 4.1mm (SS) 250 x 4.6mm (PEEK) 150 x 4.1mm (SS) 250 x 4.1mm (SS) 250 x 4.6mm (PEEK)

79805 79806 79444 79409 79529 79404 79380 79425 79427 79381

PRP®-3

300Å 300Å 300Å

3µm 10µm 10µm

150 x 4.1mm (SS) 150 x 4.1mm (SS) 250 x 10.0mm (SS)

79809 79466 79526

— — — — — —

3µm 7µm 7µm 7µm 7µm 7µm

150 x 4.1mm (SS) 150 x 2.1mm (SS) 250 x 2.1mm (SS) 150 x 4.1mm (SS) 250 x 4.1mm (SS) 150 x 4.6mm (PEEK)

79819 79396 79397 79464 79465 79485

5µm 7µm

150 x 4.1mm (SS) 150 x 4.1mm (SS)

79834 79835

PRP®-X300

PRP®-X700 100Å 100Å

PRICE

Hamilton Guard Kits and Cartridges DESCRIPTION

QTY

PART NO.

Guard Column Starter Kits PRP®-1, 1 Holder, 2 Cartridges (SS) PRP®-1, 1 Holder, 2 Cartridges (PEEK) PRP®-3, 1 Holder, 2 Cartridges (SS) PRP®-X300, 1 Holder, 2 Cartridges (SS) PRP®-X700, 1 Holder, 2 Cartridges (SS) PRP®-X700, 1 Holder, 2 Cartridges (PEEK)

Ea Ea Ea Ea Ea Ea

79447 79317 79461 79453 79840 79841

Replacement Guard Cartridges† PRP®-1, 25 x 2.3mm (SS) PRP®-1, 25 x 2.3mm (PEEK) PRP®-3, 25 x 2.3mm (SS) PRP®-X300, 25 x 2.3mm (SS) PRP®-X700, 25 x 2.3mm (SS) PRP®-X700, 25 x 2.3mm (PEEK)

5 5 5 5 5 5

79445 79318 79454 79453 79842 79843

PRICE

†

Requires use of Hamilton Guard Cartridge Holder, included in Starter Kits.

4

hplc hplc

Sulfa Drugs at pH 9.1

2,3

PORE SIZE

9147

5 67 ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

10

Sulfanilic Acid Succinylsulfathiazole Sulfadiazine Sulfamerazine Sulfisoxazole Sulfaguanidine Sulfanilybenzamide Sulfathiazole Sulfanilamide Sulfamethazine

○

○

9

1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

○

8

○

1

10 Min.

○

8

○

○

Hamilton PRP-1, 10µm, 150 x 4.1mm 100mm Sodium Borate, pH 9.1:Acetonitrile (11:1) 1.0mL/min UV at 254nm © Copyright 2003 Alltech Associates, Inc.

○

Contact your Alltech office or distributor for current or local prices.

○

○

Column: Mobile Phase: Flowrate: Detector:

6

○

4

○

2

○

0

419

Hypersil® BDS Hypersil® BDS – Versatility for the Future

Base Neutral Mixture 1 2

• Base Deactivated for Analysis of Basic Compounds • Superior Peak Shape with High Efficiency for Analysis of Neutral and Acidic Compounds • Available with C8, C18, Phenyl or CN Bonded Phase • Specially Endcapped for Added Stability

5684

4

1. 2. 3. 4. 5.

5

Hypersil® has developed BDS C8, C18, Phenyl, and CN phases specifically to provide excellent, highly reproducible chromatography (Figure 1 and 2). By a combination of proprietary silica preparation and novel bonding chemistry, Hypersil® has produced four stable reversed-phase packing materials particularly well suited for pharmaceutical and other biologically important compounds. All four phases are 130Å spherical media which has been specially endcapped for added stability.

Selectivity of 5µm Hypersil® BDS C18

3

0

4

Column: Mobile Phase: Flowrate: Detector:

Uracil Pyridine Phenetole N,N-Diethylaniline Xylene

8 Min. Hypersil® BDS C18, 5µm, 150 x 4.6mm 40% Acetonitrile 60% 0.05M Phosphate buffer, pH 4.5 1.0mL/min UV at 254nm

Hypersil® BDS Columns

10.00 8.00 6.00 4.00 0.00 2.00 -2.00 -4.00 -6.00 -8.00 -10.00

LENGTH X ID

INDUSTRY STANDARD PART NO.

BDS C18

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

11779 11780 11141 11145

BDS C8

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

11781 11782 11446 11450

BDS Phenyl

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

11355 11360 11576 11580

BDS CN

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

11563 11568 11584 11588

PARTICLE SIZE

% Deviation

PACKING

1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76

PRICE

Number of Batches (1989-1995) 2653

Figure 1 - Line graph illustrating the minimal variation in selectivity ratio on Hypersil® BDS C18, 5µm packing.

®

%C

14.00 13.50 13.00 12.50 12.00 11.50 11.00 10.50 10.00 9.50 9.00

Hypersil® BDS All-Guard Cartridges PACKING

0

10 20 30 40 50 Number of Batches (1989-1995)

60

PART NO.

PRICE

All-Guard Holder (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder Ea 80101

70

○ ○

QTY

Figure 2 - Line graph illustrating the highly reproducible carbon load results on Hypersil® BDS C18, 5µm packing.

2654

i

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

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PARTICLE SIZE LENGTH X ID

All-Guard™ Cartridges (Requires Guard Holder) BDS C18 5µm 7.5 x 4.6mm 3 96002 BDS C8 5µm 7.5 x 4.6mm 3 96004 BDS Phenyl 5µm 7.5 x 4.6mm 3 96005 BDS CN 5µm 7.5 x 4.6mm 3 96006

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hplc hplc

5µm Hypersil BDS C18 Percent Carbon Load

420

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Hypersil® BDS Hypersil® BDS – Provides Superior Peak Shape and High Efficiency Chromatographers frequently encounter problems in the reversed-phase separation of basic compounds. Chromatograms often exhibit broad tailing peaks with low plate numbers, and varying retention times as columns age. Although it is possible to reduce the tailing effects with the addition of organic modifiers like triethylamine, it is preferable to use simple binary mobile phases.

The superior peak shape and efficiency of Hypersil® BDS is demonstrated by comparing the performance of a conventional reversed-phase material with BDS-C18 (Figure 3) for the analysis of several basic compounds. Acidic compounds can interact with residual surface silanols to give similar problems to those noted with bases. Notice the excellent peak shape for both a neutral-base mix (Figure 4) and an acid mix (Figure 5) when using Hypersil® BDS Columns.

Conventional C18 1

Hypersil® BDS C18

1 5673

5674

4

2

4

3

2

1. Uracil 2. Pyridine 3. N,N-Dimethylaniline 4. Benzophenone

1. Uracil 2. Pyridine 3. N,N-Dimethylaniline 4. Benzophenone

3

0

5

Column: Mobile Phase: Flowrate: Detector:

10

15 Min.

0

5

Column: Mobile Phase: Flowrate: Detector:

Conventional C18, 5µm, 150 x 4.6mm Methanol:Water (60:40) 1.0mL/min UV at 254nm

10 Min.

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:Water (60:40) 1.0mL/min UV at 254nm

Figure 3

Acid Mixture

5

6

3

1. Uracil 2. Aniline 3. 2-Nitroaniline 4. 2,4-Dinitroaniline 5. 3,5-Xylenol 6. Anisole 7. Toluene

7

1. Glutamic Acid 2. Glycolic Acid 3. Malic Acid 4. Citric Acid 5. Succinic Acid 6. Fumaric Acid

5

1

2

4

6

8

10

12

14 Min.

0

5

10 Min.

○ ○

Hypersil BDS C8, 5µm, 250 x 4.6mm 0.2M H3PO4:Methanol (90:10) 0.8mL/min UV at 254nm

○

Column: Mobile Phase: Flowrate: Detector:

○

Hypersil BDS-C18, 5µm, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (65:35) 1.0mL/min UV at 254nm

®

○

Figure 5 © Copyright 2003 Alltech Associates, Inc.

○

Contact your Alltech office or distributor for current or local prices.

○

○

Figure 4

○

○

Column: Mobile Phase: Flowrate: Detector:

®

○

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0

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○

○

○

○

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4

4

○

3 2

7632

6

hplc hplc

7639

○

2

○

Neutral-Base Mix

○

1

421

Hypersil® BDS Hypersil® BDS – Highly Reproducible Chromatography of Acidic and Basic Compounds Bases

3

Aspirin

1 5688

1 2

0

1

2

Column: Mobile Phase: Flowrate: Detector:

5687

1. Uracil 2. Pyridine 3. Methylaniline 4. Dimethylaniline 5. 2,6-Dichloronitroaniline 6. Toluene

0

3 Min.

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (60:40) 1.0mL/min UV at 254nm

3

6 Min. Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (50:50) 1.0mL/min UV at 254nm

Column: Mobile Phase: Flowrate: Detector:

Phenols

2

1. Aspirin

Base/Neutral Mix

1 5686

1

7708

5 23 4

1. Phenol 2. 2,6-Dimethylphenol 3. 3,5-Dimethylphenol 4. 4-Nitrophenol

3

1. Dihydroxyacetone 2. Propanolol 3. Toluene 4. Doxepin 5. Dibutyl Phthalate

4

0

8

0

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:Water:Acetic Acid (40:59.3:0.7) 1.0mL/min UV at 254nm

hplc hplc

Column: Mobile Phase: Flowrate: Detector:

16 Min.

5

10 15 Min. Hypersil® BDS-C18, 5µm, 150 x 4.6mm MeOH:0.05M Na2HPO4, pH7 (75:25) 1.0mL/min UV at 254nm

Column: Mobile Phase: Flowrate: Detector:

Base/Neutral Mix

Base/Acid Mixture

1 5675

5689

2 ○

2

○

○

1. 2. 3. 4.

3

1. Caffeine 2. Aspirin 3. Benzoic Acid

3

○

○

○

1

L-Ascorbic Acid Saccharin Caffeine Sodium salicylate

0

10

20

30

40 Min.

0

4

8 Min.

Column:

Hypersil BDS-C18, 5µ, 150 x 4.6mm (Part No. 11141) Mobile Phase: 6% Acetic acid 94% Water Flowrate: 1.0mL/min Detector: UV at 254nm

Column: Mobile Phase: Flowrate: Detector:

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (40:60) 1.0mL/min UV at 254nm

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4

422

Hypersil® BDS Hypersil® BDS – Well Suited for Pharmaceutical and Biologically Important Compounds Soft Drink Additives

1 3

5

Lidocaine and Amitriptyline

1 7633

5678

8 7

1. L-Ascorbic Acid 2. Saccharin 3. Caffeine 4. Sodium Salicylate 5. Vanillin 6. Aspartame 7. Sorbic Acid 8. Benzoic Acid

6 2

4

0

5

10

Column: Mobile Phase: Flowrate: Detector:

0

20 Min.

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:1% Acetic Acid (30:70) 1.0mL/min UV at 254nm

5

Polynuclear Aromatic Hydrocarbons

2 6

3

15

7

0

1

5679

Procainamides 5681

3

12 Min.

Column: Mobile Phase: Flowrate: Detector:

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (30:70) 1.0mL/min UV at 254nm

2

1. Naphthalene 2. Fluorene 3. Phenanthrene 4. Anthracene 5. Pyrene 6. Chrysene

6

14 Min.

Column: Mobile Phase: Flowrate: Detector:

1 4

1. Lidocaine 2. Amitriptyline

2

0

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Acetonitrile:Water (75:25) 1.0mL/min UV at 254nm

6

1. Procainamide 2. N-Acetylprocainamide 3. N-Propionylprocainamide

12 Min.

Column: Mobile Phase: Flowrate: Detector:

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Acetonitrile:0.05M KH2PO4, pH3.5 (12:88) 1.0mL/min UV at 254nm

hplc hplc

B Vitamins

1 2

Paracetamol 1

5682

5683

3 1. Thiamine (B1) 2. Niacinamide (B3) 3. Riboflavin (B2)

○

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○

○

○

○

○

○

○

○

○

○

1. Paracetamol

8 Min.

0

2

4 Min.

○

4

○ ○

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Acetonitrile:0.05M KH2PO4, pH4.5 (30:70) 1.0mL/min UV at 254nm

○

Column: Mobile Phase: Flowrate: Detector:

○

Hypersil® BDS-C18, 5µm, 150 x 4.6mm Methanol:0.05M KH2PO4, pH3.5 (30:70) 1.0mL/min UV at 254nm

© Copyright 2003 Alltech Associates, Inc.

○

Contact your Alltech office or distributor for current or local prices.

○

○

Column: Mobile Phase: Flowrate: Detector:

○

○

○

0

423

Hypersil® 120 Hypersil® 120Å Columns – High Performance and Reproducibility

Xanthines 9097

• Proven Reliability • Long Column Lifetime • Wide Range of Monomeric Bonded Phases

2

Hypersil® particles are uniformly spherical, thereby offering the chromatographer many advantages. Longer column life is ensured with a stable, uniformly packed bed while high permeability guaran ble up to 20,000psig) offering excellent retention characteristics. Chemically-bonded phases are used for more than 80% of all HPLC separations. All bonded chemistries prepared on Hypersil® are manufactured under stringent conditions to ensure that true chemical bonding takes place. Temperature, environment, pretreatment of silica, and reaction protocol are all strictly controlled. A wide range of Hypersil® bonded phases are available covering most applications of both normal, reversed-phase, and ion exchange chromatography.

1. 2. 3. 4.

Theobromine Theophylline Caffeine Chlorotheophylline

3

1

4

0

1

2

Column: Mobile Phase: Flowrate: Detector:

3

4

5

6

7

8 Min.

Hypersil® ODS, 120Å, 5µm, 150 x 4.6mm 50mM Sodium Acetate, pH 5.0:Methanol (75:25) 1.0mL/min UV at 254nm

Aromatics 9098

1

Table 1 - Hypersil® 120Å Specifications 3µm — Mean Particle Diameter: 5µm — Mean Particle Diameter: 10µm — Mean Particle Diameter: Mean Pore Size: Pore Size Distribution: Surface Area: Pore Volume:

2.9 - 3.2µm 4.5 - 5.0µm 8.5 - 10.0µm 120Å ± 15Å 85% Mean ± 30Å 170m2g-1 ± 10m2g-1 0.65cm3g-1 ± 0.1cm3g-1

PHASE

ODS (C18) MOS (C8) APS (NH2)

% CARBON

PHASE

% CARBON

9.1 - 10.0 6.2 - 6.8 1.6 - 1.9

CPS (CN) Phenyl SAX

3.6 - 4.4 4.6 - 5.4 2.4 - 3.0

1. 2. 3. 4. 5.

Propylbenzene Butylbenzene o-Terphenyl Amylbenzene Triphenylene

3 4

0 Column: Mobile Phase: Flowrate: Detector:

5

10

15 Min.

Hypersil® ODS, 120Å, 5µm, 150 x 4.6mm Methanol:Water (80:20) 1.0mL/min UV at 254nm

Hypersil® 120Å Bonded Phases ODS (C18) MOS-1 (C8) MOS-2 (C8) APS-1 (NH2) APS-2 (NH2) CPS-1 (CN) CPS-2 (CN) Phenyl-1 Phenyl-2 SAS (C1) Butyl (C4) SAX

Fully endcapped C18 material, monolayer coverage and an exceptionally low level of residual silanol groups. Reversed-phase material with monolayer coverage of dimethyloctylsilyl (C8) groups which is not end-capped. Endcapped to further reduce silanols; suited to the separation of moderately polar compounds. Versatile amino phase which can be used as a weak anion exchanger with aqueous buffers. Yields improved reproducibility over the original APS Hypersil-1 material. Original high coverage cyano-propyl phase which can be operated in both normal and reversed-phase modes. Endcapped version of CPS-1; shows improved stability and better peak symmetry with polar, basic solutes. High coverage, non endcapped material for reversed-phase separations of moderately polar molecules. Endcapped version of Phenyl-1; shows better peak symmetry with basic solutes than Phenyl-1. Trimethyl (C1) reversed-phase material with ideal selectivity for polar and multifunctional compounds. Short (C4) alkyl chain reversed-phase material which is more stable than other short chain modifications. Strong anion exchanger with a quaternary ammonium functional group.

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hplc hplc

Bonded Phase Specifications Range

5 2

424

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Hypersil® 120 A Wide Range of Bonded Phases Used in Many Existing Methods Hypersil® 120Å Analytical Columns

Hypersil® 120Å Analytical Columns PACKING

MOS-1 (C8)

MOS-2 (C8)

Butyl (C4)

SAS (C1)

Phenyl-1

LENGTH X ID

Phenyl-2

5µm 5µm

150 x 4.6mm 250 x 4.6mm

11208 11213

CPS-1 (CN)

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

96551 96554 96557 96560

CPS-2 (CN)

5µm 5µm

150 x 4.6mm 250 x 4.6mm

11200 11204

APS-1 (NH2)

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

9985 9989 9993 9997

11192 11196

APS-2 (NH2)

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

96575 96578 96581 96584

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

11169 11173 11177 11181

SAX

5µm 5µm

150 x 4.6mm 250 x 4.6mm

11334 11338

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

96563 96566 96569 96572

Silica

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

9851 9855 9859 9863

LENGTH X ID

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

9867 9871 9875 9879

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

9883 9887 9891 9895

3µm 3µm 5µm 5µm

100 x 4.6mm 150 x 4.6mm 150 x 4.6mm 250 x 4.6mm

11149 11153 11157 11161

5µm 5µm

150 x 4.6mm 250 x 4.6mm

3µm 3µm 5µm 5µm 3µm 3µm 5µm 5µm

SIZE

ODS (C18)

INDUSTRY STANDARD PART NO.

INDUSTRY STANDARD PART NO.

PRICE

PACKING

SIZE

PRICE

Hypersil® 120Å Analytical Guard Cartridges

Aromatic Hydrocarbons

PACKING

PARTICLE SIZE LENGTH X ID

QTY

PART NO.

PRICE

™

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i

Other dimensions and formats available. See pages 355-361 for custom HPLC columns.

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Hypersil CPS-1(CN), 5µm, 100 x 4.6mm 1% Ethyl Acetate in Hexane 2.0mL/min UV at 254nm

Guard Holders (includes Direct-Connect Column Coupler) All-Guard Catridge Holder 80101

© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Column: Mobile Phase: Flowrate: Detector:

3 Min.

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2

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1

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0

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2

96007 96008 96009 96011 96012 96013 96014 96015 96016 96017 96018 96019 96021

hplc hplc

1. Toluene 2. Nitrobenzene 3. 2,6-Dinitrotoluene

1

All-Guard Cartridges (Requires Guard Holder) SAS C1 5µm 7.5 x 4.6mm 3 Phenyl 5µm 7.5 x 4.6mm 3 CPS-1 (CN) 5µm 7.5 x 4.6mm 3 APS NH2 5µm 7.5 x 4.6mm 3 MOS-1 C8 5µm 7.5 x 4.6mm 3 ODS C18 5µm 7.5 x 4.6mm 3 Silica 5µm 7.5 x 4.6mm 3 MOS-2 C8 5µm 7.5 x 4.6mm 3 APS-2 NH2 5µm 7.5 x 4.6mm 3 CPS-2 CN 5µm 7.5 x 4.6mm 3 Phenyl-2 5µm 7.5 x 4.6mm 3 Butyl C4 5µm 7.5 x 4.6mm 3 SAX 5µm 7.5 x 4.6mm 3

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5668

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3

425

Hypersil® 120 .eps

Nucleotides

6

34

2

1 2

9

10

1. 2. 3. 4. 5. 6.

12

8

0

5

10

5 6

1

8095

11

7

5

Brain Extract Standards

15

20

25

CMP AMP UMP GMP CDP ADP

7. 8. 9. 10. 11. 12.

UDP CTP GDP ATP UTP GTP

3

30 Min.

0

Gradient: Flowrate: Detector:

1. 5-Hydroxytryptophan 2. 5-Hydroxyindole Acetic Acid 3. 5-Hydroxytryptamine 4. Tryptophan 5. Indole Acetic Acid 6. Tryptophol 7. Indole Propionic Acid

4

Hypersil® SAX, 120Å, 5µm, 250 x 4.6mm A: 5mM NH4H2PO4, pH2.8 B: 750mM NH4H2PO4,pH 3.7 Time: 0 30 %B: 0 100 1.0mL/min UV at 254nm

Column: Mobile Phase:

7182

7

5

10

15

20 Min.

Hypersil® MOS-1, 120Å, 5µm, 250 x 4.6mm A: 75mM Na Acetate B: Methanol Time: 0 4 8 20 %B: 13 13 61 61 1.1mL/min Fluorescence Ex: 280nm Em: 350nm

Column: Mobile Phase: Gradient: Flowrate: Detector:

Separation of Corticosteroids

Base/Neutral Mix

7183

7004

2

2

5

4 3

6

1

0

5

hplc hplc

Column: Mobile Phase: Flowrate: Detector:

10 Min.

1

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Hydrocortisone Acetate Prednisone Hydrocortisone Prednisolone

5 Min. Hypersil® APS-1, 120Å, 5µm, 100 x 4.6mm Dichloromethane:Methanol (95:5) 2.0mL/min UV at 254nm

Analysis of Sugars

5 7181

7184

3

7

6

2 1

5

8

4

1

9

1. Rhamnose 2. Ribose 3. Xylose 4. Arabinose 5. Fructose 6. Glucose 7. Sucrose 8. Maltose 9. Lactose

0

5

10 Min.

0

5

10 Min.

Column: Hypersil® ODS, 120Å, 5µm, 100 x 4.6mm Mobile Phase: 50mM Ammonium Acetate, 0.1mM TBAH, pH5.5: Methanol (90:10) Detector: Deuterium Lamp Fluorimeter Ex: 232nm Em: >387nm

Column: Mobile Phase: Flowrate: Temp: Detector:

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Hypersil® APS-2, 120Å, 5µm, 100 x 3.2mm Acetonitrile:Water (80:20) 0.5mL/min Ambient RI

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1. 2. 3. 4.

Column: Mobile Phase: Flowrate: Detector:

1. Adenine 2. Adenosine 3. Tubercidin 4. Unknown 5. Unknown

4

34

0

Hypersil® ODS C18, 120Å, 5µm, 250 x 4.6mm Acetonitrile:Water (65:35) 1.0mL/min UV at 254nm

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Uracil Phenol Benzaldehyde N,N-Diethyl-m-toluamide Toluene Ethylbenzene

Nucleotides and Related Compounds in Biomedicine

3

2

1. 2. 3. 4. 5. 6.

426

Hypersil® 120 Hypersil® Columns are Available for a Wide Variety of Applications Polyaromatic Hydrocarbons from Water 1. Acenaphthalene 2. Fluorene 3. Phenanthrene

2

8 10 9

56

12

11

1 4

7640

8

12

16

20

4. Anthracene 5. Fluoranthene 6. Pyrene 7. Benz[a]anthracene 8. Benzo[b]fluoranthrene 9. Benzo[k]fluoranthrene 10. Benzo[a]pyrene 11. Dibenz[a,h]anthracene 12. Indeno[1,2,3-c,d]pyrene

24 Min.

3

3

6

9

Column: Mobile Phase: Flowrate: Detector:

2

4 5

7

0

Hypersil® MOS-1 C8, 120Å, 5µm, 150 x 4.6mm Acetonitrile:Water (70:30) 1.5mL/min UV at 254nm, 2.0 AUFS

6

6

Column: Mobile Phase: Flowrate: Detector:

12 Min. Hypersil® Phenyl-1, 120Å, 5µm, 100 x 4.6mm Methanol:Water (60:40) 1.0mL/min UV at 254nm

Steroid from Hydrocortisone First-Aid Cream

1 7643

1. Chlorotetracycline

hplc hplc

Chlorotetracycline from Ointment 1

1. Benzamide 2. Benzyl Alcohol 3. Acetophenone 4. Methyl Benzoate 5. Phenetole 6. Naphthalene 7. Benzophenone 8. Biphenyl

8

5

0 2 4 6 8 10 12 Min. Column: Mobile Phase: Flowrate: Detector:

5105

4 1 3

1. Dimethyl Phthalate 2. Diethyl Phthalate 3. Diallyl Phthalate 4. Dibutyl Phthalate 5. Diamyl Phthalate

1

15 Min.

Aromatic Ketones 7642

3

12

Hypersil® APS-1, 120Å, 10µm, 250 x 4.6mm Acetonitrile:Water (75:25) 3.0mL/min RI

Phthalate Esters from Potable Water 2

1. Ethanol 2. Glucose 3. Sucrose

1

0

Hypersil® ODS, 120Å, 5µm, 250 x 4.6mm Acetonitrile:Water (87:13) 1.2mL/min UV at 254nm, 1.0 AUFS

Column: Mobile Phase: Flowrate: Detector:

7641

2

7

4 3

0

Carbohydrates from Wine

7644

3

6 Min.

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Hypersil® ODS, 120Å, 5µm, 150 x 4.6mm Methanol:Water (70:30) 1.0mL/min UV at 254nm, 1.0 AUFS

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Column: Mobile Phase: Flowrate: Detector:

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Hypersil® ODS, 120Å, 5µm, 150 x 4.6mm 0.05M H3PO4:Acetonitrile (75:25) 1.25mL/min UV at 254nm, 1.0 AUFS

© Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Column: Mobile Phase: Flowrate: Detector:

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1. Hydrocortisone

427

Hypersil® Hypercarb® Hypersil® Hypercarb® Columns – For Unrivalled Stereo-Selective Separations

Morphine and Metabolites 1

2 9150

• Separates Closely Related Compounds Including Diastereoisomers • Provides Resolution for Compounds of Wide Ranging Polarities • Now Available in 5µm High Efficiency Packing

4 3

Hypercarb® packing's porous graphitized carbon separates diastereoisomers and geometrical isomers. It has been used in the pharmaceutical industry to isolate and quantify the trace amounts of isomers and other closely related compounds found in drug samples. Hypercarb® Columns meet the increasing demand by pharmaceutical companies for a column which will isolate every isomer present—down to impurity levels of less than 0.5%. Until the introduction of Hypercarb® Columns, such selectivity and resolving power had not been routinely and reliably achieved using conventional liquid chromatography techniques. Hypercarb® Columns exhibit high mechanical strength and has been fully optimized and conditioned to provide maximum selectivity for closely related compounds. It operates over a wide pH range because it is unaffected by aggressive mobile phases and is resistant to attack by both strong acids and strong bases. This inherent stability insures that Hypercarb® Columns fully retain their selectivity across the entire pH range. Unlike polymer-based materials, Hypercarb® packings do not shrink or swell when changing from one solvent to another.

Metoprolol Isomers 2 8096

1 1. R-isomer 2. S-isomer

0

10

Column: Mobile Phase: Flowrate: Detector:

Column: Mobile Phase: Flowrate: Detector:

6

30 Min.

Hypercarb®, 7µm, 100 x 4.6mm Methanol:Ammonium Acetate, pH 9.0 (60:40) 1.0mL/min UV at 220nm

Silica Based: Polymer: Hypercarb®:

LOW pH

HIGH pH STABILITY

ABSENCE OF COLUMN SWELLING

STABILITY

– √ √

ORGANIC SOLVENT COMPATIBILITY

– √ √

√ – √

√ – √

Hypercarb® packing's exceptional stability and solvent resistance offer clear advantages over both silica and polymeric stationary phases.

Hypercarb® Analytical Columns

8 Min.

Hypercarb®, 7µm, 100 x 4.6mm L-ZGP, 2.5mM, TEA, 0.4mM in Dichloromethane 1.0mL/min UV at 278nm

PARTICLE SIZE

LENGTH X ID

PART NO.

5µm 5µm 5µm 5µm 7µm 7µm 7µm 7µm

50 x 4.6mm 100 x 4.6mm 100 x 3.0mm 100 x 2.0mm 50 x 4.6mm 100 x 4.6mm 200 x 4.6mm 100 x 2.0mm

59854550 59864550 59863550 59862550 59854751 59864750 59824750 59862750

PRICE

Hypercarb® Guard Cartridges

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hplc hplc

4

20

Hypercarb® Specifications

Hypercarb®

2

Normorphine Morphine-3-Glucuronide Morphine-6-Glucuronide Morphine Codeine 5

PACKING

0

1. 2. 3. 4. 5.

PACKING

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C

C C

C C

C C

LENGTH X ID

QTY

PART NO.

PRICE

96025

C

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C

PARTICLE SIZE

Guard Cartridges (Requires Guard Holder) Hypercarb, (SS) 7µm 10 x 4.0mm 2

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Graphitized Carbon Surface

C C

C C

C C

C C

Guard Holders (Includes Direct-Connect™ Column Coupler) Guard Cartridge Holder Ea 96029

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C C

C C

C C

C C

C C

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C

Hypercarb® has a flat, porous, graphitic carbon molecular structure consisting of layers of aromatic ribbons held together by strong covalent bonds.

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428

© Copyright 2003 Alltech Associates, Inc.

Contact your Alltech office or distributor for current or local prices.

Hypersil® Hypercarb® Anticonvulsant Drugs

Oxygen Heterocycles in Confectionary Products

2

5645

4 5

1. Phenytoin 2. Phenobarbitone 3. Carbamazepine

1

3

1

Reference: Dr. B. J. Clark, University of Bradford, W. Yorks, UK.

3

0

0

10

Hypercarb®, 7µm, 100 x 4.6mm Methanol:Acetonitrile:0.02M K2HPO4, pH 6.4 (10:50:40) 1.0mL/min UV at 215nm

Flowrate: Detector:

4

6

4

Gradient: Flowrate: Detector:

3

Reference: Dr. A. Lea and Dr. G. Ford, Reading Scientific Services Ltd., Reading, UK.

10

12 Min.

Steroid Analogues 9149

1. 2. 3. 4.

8

7031

Hypercarb®, 7µm, 100 x 4.6mm A: Aqueous Perchloric Acid, pH1.0 B: Acetonitrile Time: 0 10 15 % B: 1 35 60 1.0mL/min UV at 280nm

Isomers of Methylhippuric Acid

2

1

2

Column: Mobile Phase:

20 Min.

Column: Mobile Phase:

6

2

1. DHHM 2. Furaneol 3. Cyclotene 4. HMF 5. HMP 6. Maltol

5651

2 3 1

2-Methylhippuric Acid Hippuric Acid 3-Methylhippuric Acid 4-Methylhippuric Acid

1. Prednisone 2. Prednisolone 3. Dexamethasone 4. Betamethasone 5. Hydrocortisone 6. Prednisolone Acetate

6

4 5

Reference: Dr. B. Hoffmann, Chemisches Landesuntersuchungsamt, Nordrhein-Westfalen, Munster, Germany

Hypercarb , 7µm, 100 x 4.6mm Water:Acetonitrile (15:85) 1.0mL/min UV at 240nm

10

20

30

9

5 4

6

2

4

7

40 Min.

0

6

8

10

12 Min.

Column: Mobile Phase: Flowrate: Detector:

®

Hypercarb , 5µm, 100 x 4.6mm Acetonitrile:1% TFA in Water (85:15) 1.0mL/min UV at 254nm © Copyright 2003 Alltech Associates, Inc.

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Contact your Alltech office or distributor for current or local prices.

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Column: Hypercarb®, 7µm, 100 x 4.6mm Mobile Phase: A: 1% Acetic Acid in Water B: Acetonitrile Gradient: Time: 0 40 % B: 10 80 Flowrate: 1.0mL/min Detector: UV at 280nm

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8

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11

9151

4. 2-methyl-4-chlorophenoxy acetic Acid (MCPA) 5. 2,4-dichlorophenoxyacetic Acid (2,4-D) 6. 4-(2-methyl-4-chlorophenoxy) butyric acid 7. 4-(2,4-dichlorophenoxy)butanoic Acid (2,4-DB) 8. Benazolin 9. Flurooxypyr 10. 2,4,5,-trichlorophenoxyacetic Acid (2,4,5-T) 10

1. Bentazone 2. Dicamba 3. Mecoprop (MCPP)

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Phenol Guaiacol m-Cresol p-Cresol o-Cresol 4-Ethylphenol 2-Ethylphenol 3,5-Xylenol 4-Ethyl Guaiacol Eugenol Internal Standard

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4

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

hplc hplc

5642

2 3

Phenoxy Acids

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Column: Mobile Phase: Flowrate: Detector:

Phenols In Whiskey 9

15 Min. ®

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Hypercarb , 7µm, 100 x 4.6mm Methanol:Water, pH 2.2 (80:20) 1.5mL/min UV at 254nm

10

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Column: Mobile Phase: Flowrate: Detector:

5

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12 Min.

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8

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4

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429

Hypersil® Miscellaneous Hypersil® Hyperprep™ HS Columns

PAH (EPA 610)

• Base-deactivated Phases in Preparative Format • Excellent Chemical and Physical Stability Hyperprep™ HS 8µm packings extend analytical quality and selectivity to preparative separations. Hyperprep™ Column's selectivity and consistency are ideal for a wide range of highpurity, high recovery preparative applications. Particle size, pore size distribution, surface area, and surface chemistry are all carefully controlled for exceptional batch-to-batch reproducibility. Hyperprep™ packing's narrow particle size distribution provides low pressure drops and high flowrates. Hyperprep™ HS silica and bonded phases are base deactivated ensuring excellent peak shape for the separation of small, basic solutes. Hyperprep™ packing's high mechanical strength prevents generation of fines in the column bed, resulting in longer column lifetime.

100 HS Silica

100 HS BDS C8

0

Hypersil® Green PAH, 5µm, 100 x 4.6mm A: Water:Acetonitrile (99:1) B: Acetonitrile:Water (99:1) Time: 0 5 25 %B: 50 50 100 2.0mL/min UV at 254nm

Column: Mobile Phase: Gradient:

Hypersil® Hyperprep™ HS Columns PACKING

7707 3. Acenaphthene 4. Fluorene 5. Phenanthrene 2 6. Anthracene 5 7. Fluoranthene 10 4 8. Pyrene 9. Benzo[a]anthracene 1 7 9 10. Chrysene 13 16 11. Benz[b]fluoranthene 12 15 3 12. Benzo[k]fluroanthene 14 8 13. Benzo[a]pyrene 14. Dibenz[a,h]anthracene 15. Benzo[g,h,i]perylene 5 10 15 20 25 Min. 16. Indeno[1,2,3,c,d]pyrene

1. Naphthalene 2. Acenaphthylene 11

6

PARTICLE SIZE

LENGTH X ID

PART NO.

8µm 8µm 8µm

250 x 4.6mm 250 x 10mm 250 x 22mm

11399 11371 11372

8µm 8µm 8µm

250 x 4.6mm 250 x 10mm 250 x 22mm

11373 11374 11375

PRICE

Flowrate: Detector:

Phenols

23 6

1. Phenol 2. 4-Nitrophenol 3. 2,4-Dinitrophenol 8 4

100 HS BDS C18

i

8µm 8µm 8µm

250 x 4.6mm 250 x 10mm 250 x 22mm

hplc hplc ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

1 0

Contact Alltech Customer Service for phases and particle sizes not listed.

Hypersil® Environmental Columns – Tailor Made for Environmental Analyses

430

10

11376 11377 11378

• Hypersil® Green ENV – for Analysis of Phenols, Phthalates, Pesticides, and Herbicides • Hypersil® Green PAH – for Analysis of Polyaromatic Hydrocarbons • Hypersil® Green Carbamate – for Analysis of Carbamate Hypersil® Green ENV is an alkyl bonded phase tailored for the analysis of polar pollutants commonly found in industrial and agricultural residues. The unique selectivity of Hypersil® Green ENV separates chloro- and nitro-phenolic residues found in pesticides and chlorinated water supplies; it also separates phthalate plasticizers. The Hypersil® Green ENV column provides highly reproducible analyses even at low sample concentration. Hypersil® Green PAH is a specially tailored alkyl bonded silica with a high carbon load that completely resolves this class of priority pollutants. The major problem in the HPLC analysis of PAH’s arises from their structural similarity. With the Hypersil® Green PAH column, all sixteen EPA method 610 PAH's are fully resolved in less than 30 minutes. The Hypersil® Green PAH column completely separates PAH’s in either isocratic or gradient elution modes. The Hypersil® Green Carbamate column resolves carbamates using either isocratic or gradient elution. This column works well with post column derivatization and fluorescence detection as detailed in EPA method 531.1. © Copyright 2003 Alltech Associates, Inc.

5 7 9 10

20

Column: Mobile Phase: Gradient: Flowrate: Detector:

11

4. 5. 6. 7. 8. 9. 10. 11.

5690

2-Nitrophenol o-Chlorophenol 4,6-Dinitrophenol 2,4-Dimethylphenol 4-Chloro-3-methylphenol 2,4-Dichlorophenol 2,4,6-Trichlorophenol Pentachlorophenol

30 Min. Hypersil® Green ENV, 5µm, 150 x 4.6mm A: 1% Acetic Acid in Water B: 1% Acetic Acid in Methanol Time: 0 20 30 %B: 35 100 100 1.0mL/min UV at 280nm

Hypersil® Environmental Columns PACKING

PARTICLE SIZE

LENGTH X ID

PART NO.

Green ENV

5µm

150 x 4.6mm

59874512

Green PAH

3µm 5µm

100 x 4.6mm 100 x 4.6mm

59864311 59864511

Green Carbamate

5µm

250 x 4.6mm

75084562

PRICE

Hypersil® Environmental Guard Columns PACKING

PARTICLE SIZE

LENGTH X ID

QTY

All-Guard Cartridges (Requires Guard Holder) Green ENV 5µm 7.5 x 4.6mm 3 Green PAH 5µm 7.5 x 4.6mm 3 Carbamate 5µm 7.5 x 4.6mm 3

PART NO.

PRICE

96027 96026 96028

Guard Holders (Includes Direct-Connect Column Coupler) All-Guard™ Cartridge Holder Ea 80101 Contact your Alltech office or distributor for current or local prices.

Jordi GPC sample passes through fewer frits reducing shearing and adsorption. And with fewer columns, operating pressures are lower.

Jordi DVB Columns Offer Durability and Performance • Rugged - 100% Divinylbenzene Packing for Use at High Temperatures and with Any Solvent • Powerful - High Pore Volume for Greater Resolution on a Single Column • Efficient - High Plate Counts for Sharp, Symmetrical Peaks • Versatile - Multiple Surface Chemistries for Organic and WaterSoluble Polymers Jordi Associates is the only company that makes GPC packings from 100% polydivinylbenzene (DVB) for compatibility with high temperatures, pressures, and a wide range of solvents. Competitors make their packings from fragile polystyrenedivinylbenzene copolymers (PS-DVB) that shrink and swell with changes in solvent, temperature, and pressure. Jordi columns, made exclusively from DVB, offer unparalleled resistance to shrinking and swelling (Figure 1). Rugged Jordi GPC columns allow flexible operating conditions for solving your toughest polymer analysis problems. Use one Jordi GPC column with toluene, THF, methanol, hexane, HFIP, acetone, Freon, or 5% water in THF. Use the same column at temperatures up to 150°C, pH’s from 0 to 14, and with samples containing salts, acids, or bases. Jordi GPC packings have higher pore volume than PS-DVB packings for more separating power per column. One 500mm x 10mm Jordi Mixed-Bed column replaces three 300mm x 7.8mm PSDVB columns and separates the same sample in 40% less time. The

All Jordi packing materials are manufactured as 5µm particles to provide the high efficiencies that GPC chromatographers demand. The small particle size coupled with the ruggedness of the packing provide greater resolution and sharp, symmetrical peaks unique to the GPC industry. Jordi GPC columns are available in several pore sizes and several different surface chemistries to solve a variety of polymer analysis problems. The native DVB polymer columns separate organic-soluble polymers. Hydrophilic Glucose-DVB, sulfonatedDVB, and Polar Pac WAX columns separate water soluble polymers. Jordi GPC columns come with pore sizes ranging from 100Å for the separation of low MW compounds (50 - 5000 Daltons) to 105Å for high MW compounds (10,000 - 10,000,000 Daltons), and MixedBed packings for wide ranging MW (100 - 10,000,000 Daltons) samples. All chemistries feature a 100% DVB backbone for durability and performance. Jordi DVB Column Specifications PORE SIZE

PRESSURE LIMIT

MW RANGE (DALTONS)

100 Å 500 Å 103 Å 104 Å 105 Å Mixed Bed

8,000psig 8,000psig 8,000psig 2,000psig 2,000psig 2,000psig