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Molisch Test

General Test for Carbohydrates Results Theoretical Actual A deep Formed a dark violet violet coloration is layer. Upon shaking, produced the entire at the solution junction of turns two layers. dark (Amrita, violet. 2011)

Tests for Aldoses and Ketoses Results Theoretical Actual Ketoses produce a The unknown produces cherry red color. a clear colorless Aldoses produces solution. yellow to faint pink color under appropriate condition.

Seliwanoff’s Test

Indication (+) The unknown sample yield a positive result for carbohydrates.

Indication (-) The unknown sample is yields a negative result.

Pentoses produces a blue green colored solution. Muddy brown colored precipitate distinguish a hexose.

The unknown sample produces a blue green color solution.

(+) The unknown sample yields a positive result for pentose.

Pentoses produces a red color solution.

The unknown sample produces a yellow clear solution. Upon heating, it turns to a a red colored solution.

(+) The uknown sample yields a positive result for pentose.

Bial’s Test

Tollen’s Phloroglucinol Test

Benedict’s Test

Tests for Reducing Sugars Results Theoretical Actual The unknown sample A green solution with a produced a brick red precipitate prove that precipitate there is a in a green trace of solution reducing after heating sugar. in a water (HiMedia bath. Lab, 2015)

Indication (+) The unknown sample has a trace of reducing sugar.

Reducing sugar will formed a brick precipitate.

Fehling’s Test

Ferricyanide Test

Phenylhydrazine Test

Upon heating, aldoses produce a yellow colored solution. Ketoses produces orange solution

Reducing sugars produce a orange or yellow-orange condensation product, or precipitate upon heating.

Table 1. Result of the following test performed for the unknown sample.

Table 2. Summary of the results

The unknown sample produced a brick red precipitate in a orange solution after heat was applied for 3 mins.

(+) The unknown sample yields a positive result for Fehling’s test in determining reducing sugar.

The unknown sample produced a yellow soluton upon heating for 10 mins and cooled.

(+) The unknown sample yields a positive result for aldoses.

The unknown sample produces an orange precipitate upon heating for 3mins.

(+) The unknown sample yields a positive result for reducing sugar as determined by phenylhydrazine test.