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CBB 40104 Bioplant Design Project 2 Exercise 2 (using superPro software) Problem Statement: Understanding cell growth rates and the effects of environmental conditions of growth is a key aspect of biological engineering. The most common and versatile method of cell growth analysis used throughout industry is batch fermentation in a bioreactor. The fermentation bioreactor enables cell growth to take place under well-defined operating conditions. Below is a typical overall of fermentation reaction.

100 Media

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70 Oxygen

28 Biomass + 70 Carbon Dioxide + 10 Impurity + 2 MAB

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60 Water

All of the feed streams enter the reactor at STP. 7800 kg of water is fed to the reactor at a flowrate of 100 L/min with a setup time of 5 minutes. 380 kg media is also fed to the reactor at a flowrate of 10 kg/min with a setup time of 20 minutes. This fermentation cycle occurs at a temperature of 37oC with a constant heat rate of 0.5oC/min. The calculated reaction rate refers to biomass formation (dX/dt) since biomass was selected as the Rate Reference Component with a max of 0.2 h-1. Media is the only substrate with a Ks value of 35 mg/L. The reaction is first order in biomass and has an enthalpy of -3697 kcal/kg at an oxygen reference temperature of 37oC. The product will be cooled to 5oC using glycol, which has a cooling rate of 0.5oC/min and will be transferred out of the fermentor in a process time of 272 minutes. Determine the amount of time required to completely consume the reaction components and what is the limiting reactant for this system.